Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing
We used next-generation sequencing (NGS) techniques to develop 14 new microsatellite loci in the great bustard ( Otis tarda ), an endangered steppe bird. We genotyped 22 individuals of the species in order to quantify levels of polymorphism and found that the number of alleles per locus ranged from...
Gespeichert in:
| Veröffentlicht in: | Conservation genetics resources 2013-06, Vol.5 (2), p.549-551 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 551 |
|---|---|
| container_issue | 2 |
| container_start_page | 549 |
| container_title | Conservation genetics resources |
| container_volume | 5 |
| creator | Horreo, J. L. Alonso, J. C. Palacín, C. Milá, B. |
| description | We used next-generation sequencing (NGS) techniques to develop 14 new microsatellite loci in the great bustard (
Otis tarda
), an endangered steppe bird. We genotyped 22 individuals of the species in order to quantify levels of polymorphism and found that the number of alleles per locus ranged from 2 to 11 (mean = 4.21), with observed heterozygosity values for each locus ranging between 0.16 and 0.82 (mean = 0.52). We found no linkage disequilibrium between marker pairs nor any departures from Hardy–Weinberg equilibrium after Bonferroni correction. This new set of markers will be useful for the study of genetic variation and structure in the great bustard and other members of the family Otididae, many of them highly vulnerable. |
| doi_str_mv | 10.1007/s12686-012-9849-5 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2406973040</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2406973040</sourcerecordid><originalsourceid>FETCH-LOGICAL-c316t-87b95eedb61a85abec2156a70032417136101e821373f1af21c635a9bc3941233</originalsourceid><addsrcrecordid>eNp1kLtOwzAUhi0EEqXwAGyWWGAI-NiJnYyo4iZV6gKz5ThO4iqNg-0MHXh3EgXBxHT-4b_ofAhdA7kHQsRDAMpznhCgSZGnRZKdoBXkQiSCcnL6qzN6ji5C2BPCc0bpCn29VaaPtrZaRet67Go8uO54cH5orcYHq70LKpqus9HgzmmLa-dxbA02faX6xnhT4cYbFXE5hqh8hW930QY8S3WHyyNubdMmsfVubNphjDiYz9H02vbNJTqrVRfM1c9do4_np_fNa7LdvbxtHreJZsBjkouyyIypSg4qz1RpNIWMK0EIoykIYBwImJwCE6wGVVPQnGWqKDUrUqCMrdHN0jt4N22HKPdu9P00KWlKeCEYScnkgsU1_xy8qeXg7UH5owQiZ8pyoSwnynKmLLMpQ5dMmLwzjb_m_0PfeQ2A-g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2406973040</pqid></control><display><type>article</type><title>Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing</title><source>Springer Nature - Complete Springer Journals</source><creator>Horreo, J. L. ; Alonso, J. C. ; Palacín, C. ; Milá, B.</creator><creatorcontrib>Horreo, J. L. ; Alonso, J. C. ; Palacín, C. ; Milá, B.</creatorcontrib><description>We used next-generation sequencing (NGS) techniques to develop 14 new microsatellite loci in the great bustard (
Otis tarda
), an endangered steppe bird. We genotyped 22 individuals of the species in order to quantify levels of polymorphism and found that the number of alleles per locus ranged from 2 to 11 (mean = 4.21), with observed heterozygosity values for each locus ranging between 0.16 and 0.82 (mean = 0.52). We found no linkage disequilibrium between marker pairs nor any departures from Hardy–Weinberg equilibrium after Bonferroni correction. This new set of markers will be useful for the study of genetic variation and structure in the great bustard and other members of the family Otididae, many of them highly vulnerable.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-012-9849-5</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Conservation Biology/Ecology ; Ecology ; Evolutionary Biology ; Genetic diversity ; Heterozygosity ; Life Sciences ; Linkage disequilibrium ; Next-generation sequencing ; Otis tarda ; Plant Genetics and Genomics ; Technical Note</subject><ispartof>Conservation genetics resources, 2013-06, Vol.5 (2), p.549-551</ispartof><rights>Springer Science+Business Media Dordrecht 2012</rights><rights>Springer Science+Business Media Dordrecht 2012.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-87b95eedb61a85abec2156a70032417136101e821373f1af21c635a9bc3941233</citedby><cites>FETCH-LOGICAL-c316t-87b95eedb61a85abec2156a70032417136101e821373f1af21c635a9bc3941233</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-012-9849-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-012-9849-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Horreo, J. L.</creatorcontrib><creatorcontrib>Alonso, J. C.</creatorcontrib><creatorcontrib>Palacín, C.</creatorcontrib><creatorcontrib>Milá, B.</creatorcontrib><title>Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>We used next-generation sequencing (NGS) techniques to develop 14 new microsatellite loci in the great bustard (
Otis tarda
), an endangered steppe bird. We genotyped 22 individuals of the species in order to quantify levels of polymorphism and found that the number of alleles per locus ranged from 2 to 11 (mean = 4.21), with observed heterozygosity values for each locus ranging between 0.16 and 0.82 (mean = 0.52). We found no linkage disequilibrium between marker pairs nor any departures from Hardy–Weinberg equilibrium after Bonferroni correction. This new set of markers will be useful for the study of genetic variation and structure in the great bustard and other members of the family Otididae, many of them highly vulnerable.</description><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Evolutionary Biology</subject><subject>Genetic diversity</subject><subject>Heterozygosity</subject><subject>Life Sciences</subject><subject>Linkage disequilibrium</subject><subject>Next-generation sequencing</subject><subject>Otis tarda</subject><subject>Plant Genetics and Genomics</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp1kLtOwzAUhi0EEqXwAGyWWGAI-NiJnYyo4iZV6gKz5ThO4iqNg-0MHXh3EgXBxHT-4b_ofAhdA7kHQsRDAMpznhCgSZGnRZKdoBXkQiSCcnL6qzN6ji5C2BPCc0bpCn29VaaPtrZaRet67Go8uO54cH5orcYHq70LKpqus9HgzmmLa-dxbA02faX6xnhT4cYbFXE5hqh8hW930QY8S3WHyyNubdMmsfVubNphjDiYz9H02vbNJTqrVRfM1c9do4_np_fNa7LdvbxtHreJZsBjkouyyIypSg4qz1RpNIWMK0EIoykIYBwImJwCE6wGVVPQnGWqKDUrUqCMrdHN0jt4N22HKPdu9P00KWlKeCEYScnkgsU1_xy8qeXg7UH5owQiZ8pyoSwnynKmLLMpQ5dMmLwzjb_m_0PfeQ2A-g</recordid><startdate>20130601</startdate><enddate>20130601</enddate><creator>Horreo, J. L.</creator><creator>Alonso, J. C.</creator><creator>Palacín, C.</creator><creator>Milá, B.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20130601</creationdate><title>Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing</title><author>Horreo, J. L. ; Alonso, J. C. ; Palacín, C. ; Milá, B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-87b95eedb61a85abec2156a70032417136101e821373f1af21c635a9bc3941233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>Genetic diversity</topic><topic>Heterozygosity</topic><topic>Life Sciences</topic><topic>Linkage disequilibrium</topic><topic>Next-generation sequencing</topic><topic>Otis tarda</topic><topic>Plant Genetics and Genomics</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horreo, J. L.</creatorcontrib><creatorcontrib>Alonso, J. C.</creatorcontrib><creatorcontrib>Palacín, C.</creatorcontrib><creatorcontrib>Milá, B.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horreo, J. L.</au><au>Alonso, J. C.</au><au>Palacín, C.</au><au>Milá, B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2013-06-01</date><risdate>2013</risdate><volume>5</volume><issue>2</issue><spage>549</spage><epage>551</epage><pages>549-551</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>We used next-generation sequencing (NGS) techniques to develop 14 new microsatellite loci in the great bustard (
Otis tarda
), an endangered steppe bird. We genotyped 22 individuals of the species in order to quantify levels of polymorphism and found that the number of alleles per locus ranged from 2 to 11 (mean = 4.21), with observed heterozygosity values for each locus ranging between 0.16 and 0.82 (mean = 0.52). We found no linkage disequilibrium between marker pairs nor any departures from Hardy–Weinberg equilibrium after Bonferroni correction. This new set of markers will be useful for the study of genetic variation and structure in the great bustard and other members of the family Otididae, many of them highly vulnerable.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-012-9849-5</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1877-7252 |
| ispartof | Conservation genetics resources, 2013-06, Vol.5 (2), p.549-551 |
| issn | 1877-7252 1877-7260 |
| language | eng |
| recordid | cdi_proquest_journals_2406973040 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Evolutionary Biology Genetic diversity Heterozygosity Life Sciences Linkage disequilibrium Next-generation sequencing Otis tarda Plant Genetics and Genomics Technical Note |
| title | Identification of polymorphic microsatellite loci for the endangered great bustard (Otis tarda) by high-throughput sequencing |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-21T18%3A23%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20polymorphic%20microsatellite%20loci%20for%20the%20endangered%20great%20bustard%20(Otis%20tarda)%20by%20high-throughput%20sequencing&rft.jtitle=Conservation%20genetics%20resources&rft.au=Horreo,%20J.%20L.&rft.date=2013-06-01&rft.volume=5&rft.issue=2&rft.spage=549&rft.epage=551&rft.pages=549-551&rft.issn=1877-7252&rft.eissn=1877-7260&rft_id=info:doi/10.1007/s12686-012-9849-5&rft_dat=%3Cproquest_cross%3E2406973040%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2406973040&rft_id=info:pmid/&rfr_iscdi=true |