A marker suitable for sex-typing birds from degraded samples
A new primer set was developed for sex-typing birds, Z37B . This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, r...
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| Veröffentlicht in: | Conservation genetics resources 2015-06, Vol.7 (2), p.337-343 |
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| creator | Dawson, Deborah A. Brekke, Patricia Dos Remedios, Natalie Horsburgh, Gavin J. |
| description | A new primer set was developed for sex-typing birds,
Z37B
. This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, rails, seabirds, eagles and the brown kiwi
Apteryx australis
(allele size range =81–103 bp), and is therefore expected to be suitable for sex-typing a wide range of species.
Z37B
sex-typed non-degraded samples (blood), degraded tissue (dead unhatched embryos, dead nestlings and museum specimens) and samples of low quantity DNA (plucked feathers and buccal swabs). The small amplicon sizes in birds suggest that this marker will be of utility for sex-typing feathers, swabs and degraded samples from a wide range of avian species. |
| doi_str_mv | 10.1007/s12686-015-0429-3 |
| format | Article |
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Z37B
. This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, rails, seabirds, eagles and the brown kiwi
Apteryx australis
(allele size range =81–103 bp), and is therefore expected to be suitable for sex-typing a wide range of species.
Z37B
sex-typed non-degraded samples (blood), degraded tissue (dead unhatched embryos, dead nestlings and museum specimens) and samples of low quantity DNA (plucked feathers and buccal swabs). The small amplicon sizes in birds suggest that this marker will be of utility for sex-typing feathers, swabs and degraded samples from a wide range of avian species.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-015-0429-3</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alleles ; Animal Genetics and Genomics ; Apteryx australis ; Biodiversity ; Biomedical and Life Sciences ; Birds ; Conservation Biology/Ecology ; Ecology ; Embryos ; Evolutionary Biology ; Feathers ; Life Sciences ; Plant Genetics and Genomics ; Sex ; Species ; Technical Note ; Typing</subject><ispartof>Conservation genetics resources, 2015-06, Vol.7 (2), p.337-343</ispartof><rights>The Author(s) 2015</rights><rights>The Author(s) 2015. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-bb079d6d19b984d8391b5a1cca9307f79e692e1bc72e9e5449ac0e12c718cf013</citedby><cites>FETCH-LOGICAL-c359t-bb079d6d19b984d8391b5a1cca9307f79e692e1bc72e9e5449ac0e12c718cf013</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-015-0429-3$$EPDF$$P50$$Gspringer$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-015-0429-3$$EHTML$$P50$$Gspringer$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids></links><search><creatorcontrib>Dawson, Deborah A.</creatorcontrib><creatorcontrib>Brekke, Patricia</creatorcontrib><creatorcontrib>Dos Remedios, Natalie</creatorcontrib><creatorcontrib>Horsburgh, Gavin J.</creatorcontrib><title>A marker suitable for sex-typing birds from degraded samples</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>A new primer set was developed for sex-typing birds,
Z37B
. This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, rails, seabirds, eagles and the brown kiwi
Apteryx australis
(allele size range =81–103 bp), and is therefore expected to be suitable for sex-typing a wide range of species.
Z37B
sex-typed non-degraded samples (blood), degraded tissue (dead unhatched embryos, dead nestlings and museum specimens) and samples of low quantity DNA (plucked feathers and buccal swabs). The small amplicon sizes in birds suggest that this marker will be of utility for sex-typing feathers, swabs and degraded samples from a wide range of avian species.</description><subject>Alleles</subject><subject>Animal Genetics and Genomics</subject><subject>Apteryx australis</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Birds</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Embryos</subject><subject>Evolutionary Biology</subject><subject>Feathers</subject><subject>Life Sciences</subject><subject>Plant Genetics and Genomics</subject><subject>Sex</subject><subject>Species</subject><subject>Technical Note</subject><subject>Typing</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM1LAzEQxYMoWKt_gLeA5-gk2U024KUUv6DgRc8hH7PL1ra7Jluw_71bVvTkaWbgvTe8HyHXHG45gL7LXKhKMeAlg0IYJk_IjFdaMy0UnP7upTgnFzmvAVQlhZiR-wXduvSBieZ9Ozi_QVp344FfbDj07a6hvk0x0zp1WxqxSS5ipNlt-w3mS3JWu03Gq585J--PD2_LZ7Z6fXpZLlYsyNIMzHvQJqrIjTdVEStpuC8dD8EZCbrWBpURyH3QAg2WRWFcAOQiaF6FGrick5spt0_d5x7zYNfdPu3Gl1YUoIyG0shRxSdVSF3OCWvbp3Ysd7Ac7BGSnSDZEZI9QrJHj5g8edTuGkx_yf-bvgGbxmjZ</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Dawson, Deborah A.</creator><creator>Brekke, Patricia</creator><creator>Dos Remedios, Natalie</creator><creator>Horsburgh, Gavin J.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>C6C</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20150601</creationdate><title>A marker suitable for sex-typing birds from degraded samples</title><author>Dawson, Deborah A. ; Brekke, Patricia ; Dos Remedios, Natalie ; Horsburgh, Gavin J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-bb079d6d19b984d8391b5a1cca9307f79e692e1bc72e9e5449ac0e12c718cf013</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Apteryx australis</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Birds</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Embryos</topic><topic>Evolutionary Biology</topic><topic>Feathers</topic><topic>Life Sciences</topic><topic>Plant Genetics and Genomics</topic><topic>Sex</topic><topic>Species</topic><topic>Technical Note</topic><topic>Typing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dawson, Deborah A.</creatorcontrib><creatorcontrib>Brekke, Patricia</creatorcontrib><creatorcontrib>Dos Remedios, Natalie</creatorcontrib><creatorcontrib>Horsburgh, Gavin J.</creatorcontrib><collection>Springer Nature OA Free Journals</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dawson, Deborah A.</au><au>Brekke, Patricia</au><au>Dos Remedios, Natalie</au><au>Horsburgh, Gavin J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A marker suitable for sex-typing birds from degraded samples</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2015-06-01</date><risdate>2015</risdate><volume>7</volume><issue>2</issue><spage>337</spage><epage>343</epage><pages>337-343</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>A new primer set was developed for sex-typing birds,
Z37B
. This primer set was designed to amplify alleles of small size to render it suitable for sex-typing degraded samples, including shed feathers. This marker successfully sex-typed 50 % of the species tested, including passerines, shorebirds, rails, seabirds, eagles and the brown kiwi
Apteryx australis
(allele size range =81–103 bp), and is therefore expected to be suitable for sex-typing a wide range of species.
Z37B
sex-typed non-degraded samples (blood), degraded tissue (dead unhatched embryos, dead nestlings and museum specimens) and samples of low quantity DNA (plucked feathers and buccal swabs). The small amplicon sizes in birds suggest that this marker will be of utility for sex-typing feathers, swabs and degraded samples from a wide range of avian species.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-015-0429-3</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1877-7252 |
| ispartof | Conservation genetics resources, 2015-06, Vol.7 (2), p.337-343 |
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| subjects | Alleles Animal Genetics and Genomics Apteryx australis Biodiversity Biomedical and Life Sciences Birds Conservation Biology/Ecology Ecology Embryos Evolutionary Biology Feathers Life Sciences Plant Genetics and Genomics Sex Species Technical Note Typing |
| title | A marker suitable for sex-typing birds from degraded samples |
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