Development of microsatellites for Southern Darwin’s frog Rhinoderma darwinii (Duméril & Bibron, 1841)
The Southern Darwin’s frog Rhinoderma darwinii is a charismatic, mouth-brooding amphibian endemic to temperate forests of South America with a Vulnerable conservation status according to the IUCN Red List. We developed microsatellite markers from next generation sequence data that will aid genetic m...
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| Veröffentlicht in: | Conservation genetics resources 2014-12, Vol.6 (4), p.1035-1037 |
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| creator | Fuentes-Navarrete, Marina Gomez-Uchida, Daniel Gallardo-Escarate, Cristian Canales-Aguirre, Cristian B Galleguillos, Ricardo Ortiz, Juan Carlos |
| description | The Southern Darwin’s frog Rhinoderma darwinii is a charismatic, mouth-brooding amphibian endemic to temperate forests of South America with a Vulnerable conservation status according to the IUCN Red List. We developed microsatellite markers from next generation sequence data that will aid genetic monitoring during and after re-introduction efforts. Using bioinformatics we characterized 3,521 perfect microsatellite repeats and designed primers for 35 of them. From these, 23 were polymorphic and amplified reliably. Number of alleles varied between 2 and 15, allele sizes varied between 84 and 299 bp, and observed heterozygosities varied between 0.105 and 0.904. These microsatellites represent a valuable resource to aid recovery of threatened Southern Darwin’s frog populations. |
| doi_str_mv | 10.1007/s12686-014-0279-4 |
| format | Article |
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| subjects | Alleles Animal Genetics and Genomics Biodiversity Bioinformatics Biomedical and Life Sciences Conservation Biology/Ecology Conservation status Ecology Evolutionary Biology Frogs Genetic markers Life Sciences Microsatellite Letters microsatellite repeats Microsatellites monitoring Plant Genetics and Genomics Rhinoderma darwinii temperate forests |
| title | Development of microsatellites for Southern Darwin’s frog Rhinoderma darwinii (Duméril & Bibron, 1841) |
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