Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods
We developed ten polymorphic microsatellite markers for two species of freshwater anomura endemic to Chile, Aegla araucaniensis and Aegla pewenchae , using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variat...
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| Veröffentlicht in: | Conservation genetics resources 2012-03, Vol.4 (1), p.93-96 |
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| creator | Gouin, Nicolas Bórquez, Jéssica Samollow, Paul B. Douglas, Kory C. Jasti, Madhuri Bertin, Angéline |
| description | We developed ten polymorphic microsatellite markers for two species of freshwater anomura endemic to Chile,
Aegla araucaniensis
and
Aegla pewenchae
, using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variation. The number of alleles per locus ranged from 2 to 13 and the observed heterozygosity from 0.103 to 0.929. All the markers appeared to segregate independently. No departure from Hardy–Weinberg equilibrium was found in
A. araucaniensis
while three markers showed evidence for deficits of heterozygotes and presence of null alleles in
A. pewenchae
. These markers will be useful for population genetics analyses. |
| doi_str_mv | 10.1007/s12686-011-9482-8 |
| format | Article |
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Aegla araucaniensis
and
Aegla pewenchae
, using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variation. The number of alleles per locus ranged from 2 to 13 and the observed heterozygosity from 0.103 to 0.929. All the markers appeared to segregate independently. No departure from Hardy–Weinberg equilibrium was found in
A. araucaniensis
while three markers showed evidence for deficits of heterozygotes and presence of null alleles in
A. pewenchae
. These markers will be useful for population genetics analyses.</description><identifier>ISSN: 1877-7252</identifier><identifier>EISSN: 1877-7260</identifier><identifier>DOI: 10.1007/s12686-011-9482-8</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Alleles ; Animal Genetics and Genomics ; Biodiversity ; Biomedical and Life Sciences ; Conservation Biology/Ecology ; Ecology ; Endemic species ; Evolutionary Biology ; Genetic diversity ; Genetic markers ; Heterozygotes ; Life Sciences ; Microsatellites ; Plant Genetics and Genomics ; Population genetics ; Technical Note</subject><ispartof>Conservation genetics resources, 2012-03, Vol.4 (1), p.93-96</ispartof><rights>Springer Science+Business Media B.V. 2011</rights><rights>Springer Science+Business Media B.V. 2011.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c316t-2abf6b92d3c21208570518e4f8875637f3f65f3683d5ed6aa68f6879fcd77d403</citedby><cites>FETCH-LOGICAL-c316t-2abf6b92d3c21208570518e4f8875637f3f65f3683d5ed6aa68f6879fcd77d403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12686-011-9482-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12686-011-9482-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27903,27904,41467,42536,51297</link.rule.ids></links><search><creatorcontrib>Gouin, Nicolas</creatorcontrib><creatorcontrib>Bórquez, Jéssica</creatorcontrib><creatorcontrib>Samollow, Paul B.</creatorcontrib><creatorcontrib>Douglas, Kory C.</creatorcontrib><creatorcontrib>Jasti, Madhuri</creatorcontrib><creatorcontrib>Bertin, Angéline</creatorcontrib><title>Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods</title><title>Conservation genetics resources</title><addtitle>Conservation Genet Resour</addtitle><description>We developed ten polymorphic microsatellite markers for two species of freshwater anomura endemic to Chile,
Aegla araucaniensis
and
Aegla pewenchae
, using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variation. The number of alleles per locus ranged from 2 to 13 and the observed heterozygosity from 0.103 to 0.929. All the markers appeared to segregate independently. No departure from Hardy–Weinberg equilibrium was found in
A. araucaniensis
while three markers showed evidence for deficits of heterozygotes and presence of null alleles in
A. pewenchae
. These markers will be useful for population genetics analyses.</description><subject>Alleles</subject><subject>Animal Genetics and Genomics</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Conservation Biology/Ecology</subject><subject>Ecology</subject><subject>Endemic species</subject><subject>Evolutionary Biology</subject><subject>Genetic diversity</subject><subject>Genetic markers</subject><subject>Heterozygotes</subject><subject>Life Sciences</subject><subject>Microsatellites</subject><subject>Plant Genetics and Genomics</subject><subject>Population genetics</subject><subject>Technical Note</subject><issn>1877-7252</issn><issn>1877-7260</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM9KAzEQhxdRsFYfwFvAix5Wk2w3yR5L8U9BUETPId2dtKnbpGZ2KT6Gb2zKip48zTB8vxnmy7JzRq8ZpfIGGRdK5JSxvJoonquDbMSUlLnkgh7-9iU_zk4Q15QKVXA-yr7mGFrTueBJsGTj6hjQdNC2rgOyMfEdIhIbIul2gcxWrgXjiY2Aq13CIjE-bPqYZriF2gGSyyksW0NMNH1tvAOPDhPVkGG-hR34emXgivTo_JI8z17yhUFoyAa6VWjwNDuypkU4-6nj7O3u9nX2kD8-3c9n08e8Lpjocm4WViwq3hQ1Z5yqUtKSKZhYpWQpCmkLK0pbpDebEhphjFBWKFnZupGymdBinF0Me7cxfPSAnV6HPvp0UvMJFVVKVjJRbKD2YjCC1dvokpdPzajem9eDeZ3M6715rVKGDxlMrF9C_Nv8f-gbFkKIQg</recordid><startdate>20120301</startdate><enddate>20120301</enddate><creator>Gouin, Nicolas</creator><creator>Bórquez, Jéssica</creator><creator>Samollow, Paul B.</creator><creator>Douglas, Kory C.</creator><creator>Jasti, Madhuri</creator><creator>Bertin, Angéline</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20120301</creationdate><title>Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods</title><author>Gouin, Nicolas ; Bórquez, Jéssica ; Samollow, Paul B. ; Douglas, Kory C. ; Jasti, Madhuri ; Bertin, Angéline</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c316t-2abf6b92d3c21208570518e4f8875637f3f65f3683d5ed6aa68f6879fcd77d403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation Biology/Ecology</topic><topic>Ecology</topic><topic>Endemic species</topic><topic>Evolutionary Biology</topic><topic>Genetic diversity</topic><topic>Genetic markers</topic><topic>Heterozygotes</topic><topic>Life Sciences</topic><topic>Microsatellites</topic><topic>Plant Genetics and Genomics</topic><topic>Population genetics</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gouin, Nicolas</creatorcontrib><creatorcontrib>Bórquez, Jéssica</creatorcontrib><creatorcontrib>Samollow, Paul B.</creatorcontrib><creatorcontrib>Douglas, Kory C.</creatorcontrib><creatorcontrib>Jasti, Madhuri</creatorcontrib><creatorcontrib>Bertin, Angéline</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gouin, Nicolas</au><au>Bórquez, Jéssica</au><au>Samollow, Paul B.</au><au>Douglas, Kory C.</au><au>Jasti, Madhuri</au><au>Bertin, Angéline</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2012-03-01</date><risdate>2012</risdate><volume>4</volume><issue>1</issue><spage>93</spage><epage>96</epage><pages>93-96</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>We developed ten polymorphic microsatellite markers for two species of freshwater anomura endemic to Chile,
Aegla araucaniensis
and
Aegla pewenchae
, using two PCR-based methods, PIMA and ISSR-PCR. Nine markers per species gave useful PCR products, revealing moderate to high levels of genetic variation. The number of alleles per locus ranged from 2 to 13 and the observed heterozygosity from 0.103 to 0.929. All the markers appeared to segregate independently. No departure from Hardy–Weinberg equilibrium was found in
A. araucaniensis
while three markers showed evidence for deficits of heterozygotes and presence of null alleles in
A. pewenchae
. These markers will be useful for population genetics analyses.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12686-011-9482-8</doi><tpages>4</tpages></addata></record> |
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| ispartof | Conservation genetics resources, 2012-03, Vol.4 (1), p.93-96 |
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| source | SpringerLink Journals - AutoHoldings |
| subjects | Alleles Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Biology/Ecology Ecology Endemic species Evolutionary Biology Genetic diversity Genetic markers Heterozygotes Life Sciences Microsatellites Plant Genetics and Genomics Population genetics Technical Note |
| title | Isolation of microsatellite markers for two Chilean freshwater anomuran species (Aegla araucaniensis and Aegla pewenchae) using PCR-based methods |
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