Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing
We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per loc...
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Veröffentlicht in: | Conservation genetics resources 2014-12, Vol.6 (4), p.833-836 |
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description | We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per locus ranged from 7 to 24, observed heterozygosity ranged from 0.217 to 0.875, and the probability of identity values ranged from 0.006 to 0.133. These new loci will provide tools for examining population genetic structure, estimating effective population size and provide information to fisheries management and conservation. |
doi_str_mv | 10.1007/s12686-014-0281-x |
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Galleguillos, Ricardo ; Canales-Aguirre, Cristian B ; Love, Cara N ; Jones, Kenneth L ; Lance, Stacey L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c292t-f550422379325c1be3e7c4ac4077553dc082b33cdb217a330dbf13b0da71c5953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>alleles</topic><topic>Animal Genetics and Genomics</topic><topic>Biodiversity</topic><topic>Biomedical and Life Sciences</topic><topic>Conservation</topic><topic>Conservation Biology/Ecology</topic><topic>Conservation status</topic><topic>Ecology</topic><topic>Evolutionary Biology</topic><topic>fisheries management</topic><topic>Fishery management</topic><topic>Fjords</topic><topic>Genetic markers</topic><topic>Genetic structure</topic><topic>Heterozygosity</topic><topic>Life Sciences</topic><topic>loci</topic><topic>marine resources</topic><topic>microsatellite repeats</topic><topic>Microsatellites</topic><topic>Plant Genetics and Genomics</topic><topic>Population genetics</topic><topic>population size</topic><topic>probability</topic><topic>Sprattus</topic><topic>Sprattus sprattus</topic><topic>Technical Note</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ferrada-Fuentes, Sandra</creatorcontrib><creatorcontrib>Galleguillos, Ricardo</creatorcontrib><creatorcontrib>Canales-Aguirre, Cristian B</creatorcontrib><creatorcontrib>Love, Cara N</creatorcontrib><creatorcontrib>Jones, Kenneth L</creatorcontrib><creatorcontrib>Lance, Stacey L</creatorcontrib><collection>AGRIS</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Conservation genetics resources</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ferrada-Fuentes, Sandra</au><au>Galleguillos, Ricardo</au><au>Canales-Aguirre, Cristian B</au><au>Love, Cara N</au><au>Jones, Kenneth L</au><au>Lance, Stacey L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing</atitle><jtitle>Conservation genetics resources</jtitle><stitle>Conservation Genet Resour</stitle><date>2014-12-01</date><risdate>2014</risdate><volume>6</volume><issue>4</issue><spage>833</spage><epage>836</epage><pages>833-836</pages><issn>1877-7252</issn><eissn>1877-7260</eissn><abstract>We isolated and characterized a total of 33 microsatellite loci from the Patagonian sprat Sprattus fuegensis, a recent exploited marine resource with a conservation status unknowing. Loci were screened in 24 individuals from the inshore waters of the Aysén Fjord, Chile. The number of alleles per locus ranged from 7 to 24, observed heterozygosity ranged from 0.217 to 0.875, and the probability of identity values ranged from 0.006 to 0.133. These new loci will provide tools for examining population genetic structure, estimating effective population size and provide information to fisheries management and conservation.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><doi>10.1007/s12686-014-0281-x</doi><tpages>4</tpages></addata></record> |
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subjects | alleles Animal Genetics and Genomics Biodiversity Biomedical and Life Sciences Conservation Conservation Biology/Ecology Conservation status Ecology Evolutionary Biology fisheries management Fishery management Fjords Genetic markers Genetic structure Heterozygosity Life Sciences loci marine resources microsatellite repeats Microsatellites Plant Genetics and Genomics Population genetics population size probability Sprattus Sprattus sprattus Technical Note |
title | Development and characterization of thirty-three microsatellite markers for the Patagonian sprat, Sprattus fuegensis (Jenyns, 1842), using paired-end Illumina shotgun sequencing |
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