Zirconium–Metalloporphyrin Frameworks–Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity
We propose a novel competitive mechanism involving the dissolved oxygen (O2) between zirconium-based porphyrinic metal–organic framework nanoparticles (NMOFs) and luminol into a ratiometric electrochemiluminescence (ECL) biosensing interface. Zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) in NMOFs...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2020-05, Vol.92 (10), p.7354-7362 |
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| creator | Zhang, Guangyao Chai, Huining Tian, Mingwei Zhu, Shifeng Qu, Lijun Zhang, Xueji |
| description | We propose a novel competitive mechanism involving the dissolved oxygen (O2) between zirconium-based porphyrinic metal–organic framework nanoparticles (NMOFs) and luminol into a ratiometric electrochemiluminescence (ECL) biosensing interface. Zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) in NMOFs as electron media reduce O2 into reactive oxygen species (ROS) and produce singlet oxygen (1O2), resulting in cathodic ECL. Meanwhile, ROS also react with the luminol anion radical and amplify the anodic ECL emission. Based on the competitive-mechanism-driven ECL process, taking the detection of polynucleotide kinase (PNK) as example, with assembling DNA-functionalized NMOFs on the sensing interface, a lower detection limit of 6.5 × 10–5 U mL–1 and broader linear relationship range from 0.0002 to 10 U mL–1 were obtained compared with that of single-signal-driven ECL sensors. This proposed MOFs-luminol competitive ECL mechanism involving dissolved O2 may provide a new pathway for further research of a green and highly sensitive ECL biosensing system. |
| doi_str_mv | 10.1021/acs.analchem.0c01262 |
| format | Article |
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Zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) in NMOFs as electron media reduce O2 into reactive oxygen species (ROS) and produce singlet oxygen (1O2), resulting in cathodic ECL. Meanwhile, ROS also react with the luminol anion radical and amplify the anodic ECL emission. Based on the competitive-mechanism-driven ECL process, taking the detection of polynucleotide kinase (PNK) as example, with assembling DNA-functionalized NMOFs on the sensing interface, a lower detection limit of 6.5 × 10–5 U mL–1 and broader linear relationship range from 0.0002 to 10 U mL–1 were obtained compared with that of single-signal-driven ECL sensors. This proposed MOFs-luminol competitive ECL mechanism involving dissolved O2 may provide a new pathway for further research of a green and highly sensitive ECL biosensing system.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.0c01262</identifier><identifier>PMID: 32319281</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Biosensing Techniques ; Biosensors ; Chemistry ; Competition ; Deoxyribonucleic acid ; Dissolved oxygen ; DNA ; Electrochemical Techniques ; Electrochemiluminescence ; Enzyme Inhibitors - pharmacology ; HeLa Cells ; Humans ; Kinases ; Luminescent Measurements ; Luminol ; Metal-organic frameworks ; Metal-Organic Frameworks - chemical synthesis ; Metal-Organic Frameworks - chemistry ; Metalloporphyrins ; Molecular Structure ; Nanoparticles ; Nanoparticles - chemistry ; Oxygen ; Particle Size ; Polynucleotide 5'-Hydroxyl-Kinase - analysis ; Polynucleotide 5'-Hydroxyl-Kinase - antagonists & inhibitors ; Polynucleotide 5'-Hydroxyl-Kinase - metabolism ; Polynucleotide kinase ; Reactive oxygen species ; Singlet oxygen ; Surface Properties ; Zirconium</subject><ispartof>Analytical chemistry (Washington), 2020-05, Vol.92 (10), p.7354-7362</ispartof><rights>Copyright American Chemical Society May 19, 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a376t-4cfb4652423e348cf7287f45d9d512e592012f6f3006154f6bc91139659f9d9d3</citedby><cites>FETCH-LOGICAL-a376t-4cfb4652423e348cf7287f45d9d512e592012f6f3006154f6bc91139659f9d9d3</cites><orcidid>0000-0002-7159-5166 ; 0000-0002-0951-4222 ; 0000-0002-0041-126X ; 0000-0002-0035-3821 ; 0000-0002-7267-2952</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.0c01262$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.0c01262$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32319281$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Guangyao</creatorcontrib><creatorcontrib>Chai, Huining</creatorcontrib><creatorcontrib>Tian, Mingwei</creatorcontrib><creatorcontrib>Zhu, Shifeng</creatorcontrib><creatorcontrib>Qu, Lijun</creatorcontrib><creatorcontrib>Zhang, Xueji</creatorcontrib><title>Zirconium–Metalloporphyrin Frameworks–Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>We propose a novel competitive mechanism involving the dissolved oxygen (O2) between zirconium-based porphyrinic metal–organic framework nanoparticles (NMOFs) and luminol into a ratiometric electrochemiluminescence (ECL) biosensing interface. Zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) in NMOFs as electron media reduce O2 into reactive oxygen species (ROS) and produce singlet oxygen (1O2), resulting in cathodic ECL. Meanwhile, ROS also react with the luminol anion radical and amplify the anodic ECL emission. Based on the competitive-mechanism-driven ECL process, taking the detection of polynucleotide kinase (PNK) as example, with assembling DNA-functionalized NMOFs on the sensing interface, a lower detection limit of 6.5 × 10–5 U mL–1 and broader linear relationship range from 0.0002 to 10 U mL–1 were obtained compared with that of single-signal-driven ECL sensors. This proposed MOFs-luminol competitive ECL mechanism involving dissolved O2 may provide a new pathway for further research of a green and highly sensitive ECL biosensing system.</description><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Chemistry</subject><subject>Competition</subject><subject>Deoxyribonucleic acid</subject><subject>Dissolved oxygen</subject><subject>DNA</subject><subject>Electrochemical Techniques</subject><subject>Electrochemiluminescence</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Kinases</subject><subject>Luminescent Measurements</subject><subject>Luminol</subject><subject>Metal-organic frameworks</subject><subject>Metal-Organic Frameworks - chemical synthesis</subject><subject>Metal-Organic Frameworks - chemistry</subject><subject>Metalloporphyrins</subject><subject>Molecular Structure</subject><subject>Nanoparticles</subject><subject>Nanoparticles - chemistry</subject><subject>Oxygen</subject><subject>Particle Size</subject><subject>Polynucleotide 5'-Hydroxyl-Kinase - analysis</subject><subject>Polynucleotide 5'-Hydroxyl-Kinase - antagonists & inhibitors</subject><subject>Polynucleotide 5'-Hydroxyl-Kinase - metabolism</subject><subject>Polynucleotide kinase</subject><subject>Reactive oxygen species</subject><subject>Singlet oxygen</subject><subject>Surface Properties</subject><subject>Zirconium</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1u2zAUhIkiReP83KAoCHQt55GUaGkZuPlDHLQomk02Ak0_IkwpUSWpBN7lDOkNe5LSsJNlVlzMN_OIGUI-M5gy4OxE6ThVvXL6HrspaGBc8g9kwioOhaxrvkcmACAKPgPYJwcxPgAwBkx-IvuCC9bwmk3Iy50N2vd27P49_73BpJzzgw_D_TrYnp4H1eGTD79jVhdjZ3vv6Nx3Ayab7CPSM4c6Bb_5g3UbHaPGXiM1PtCfKlnfYQpW02-YMml9T72hP7xb96N26JNdIb22vYpIT7P-aNP6iHw0ykU83r2H5Pb87Nf8slh8v7iany4KJWYyFaU2y1JWvOQCRVlrM-P1zJTVqllVjGPV8FyJkUYASFaVRi51w5hoZNWYJkPikHzd5g7B_xkxpvbBjyE3GlteghTQyJpnqtxSOvgYA5p2CLZTYd0yaDdDtHmI9nWIdjdEtn3ZhY_LDldvptfmMwBbYGN_O_xu5n98qJ1N</recordid><startdate>20200519</startdate><enddate>20200519</enddate><creator>Zhang, Guangyao</creator><creator>Chai, Huining</creator><creator>Tian, Mingwei</creator><creator>Zhu, Shifeng</creator><creator>Qu, Lijun</creator><creator>Zhang, Xueji</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-7159-5166</orcidid><orcidid>https://orcid.org/0000-0002-0951-4222</orcidid><orcidid>https://orcid.org/0000-0002-0041-126X</orcidid><orcidid>https://orcid.org/0000-0002-0035-3821</orcidid><orcidid>https://orcid.org/0000-0002-7267-2952</orcidid></search><sort><creationdate>20200519</creationdate><title>Zirconium–Metalloporphyrin Frameworks–Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity</title><author>Zhang, Guangyao ; Chai, Huining ; Tian, Mingwei ; Zhu, Shifeng ; Qu, Lijun ; Zhang, Xueji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a376t-4cfb4652423e348cf7287f45d9d512e592012f6f3006154f6bc91139659f9d9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Chemistry</topic><topic>Competition</topic><topic>Deoxyribonucleic acid</topic><topic>Dissolved oxygen</topic><topic>DNA</topic><topic>Electrochemical Techniques</topic><topic>Electrochemiluminescence</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Kinases</topic><topic>Luminescent Measurements</topic><topic>Luminol</topic><topic>Metal-organic frameworks</topic><topic>Metal-Organic Frameworks - chemical synthesis</topic><topic>Metal-Organic Frameworks - chemistry</topic><topic>Metalloporphyrins</topic><topic>Molecular Structure</topic><topic>Nanoparticles</topic><topic>Nanoparticles - chemistry</topic><topic>Oxygen</topic><topic>Particle Size</topic><topic>Polynucleotide 5'-Hydroxyl-Kinase - analysis</topic><topic>Polynucleotide 5'-Hydroxyl-Kinase - antagonists & inhibitors</topic><topic>Polynucleotide 5'-Hydroxyl-Kinase - metabolism</topic><topic>Polynucleotide kinase</topic><topic>Reactive oxygen species</topic><topic>Singlet oxygen</topic><topic>Surface Properties</topic><topic>Zirconium</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Guangyao</creatorcontrib><creatorcontrib>Chai, Huining</creatorcontrib><creatorcontrib>Tian, Mingwei</creatorcontrib><creatorcontrib>Zhu, Shifeng</creatorcontrib><creatorcontrib>Qu, Lijun</creatorcontrib><creatorcontrib>Zhang, Xueji</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Guangyao</au><au>Chai, Huining</au><au>Tian, Mingwei</au><au>Zhu, Shifeng</au><au>Qu, Lijun</au><au>Zhang, Xueji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zirconium–Metalloporphyrin Frameworks–Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2020-05-19</date><risdate>2020</risdate><volume>92</volume><issue>10</issue><spage>7354</spage><epage>7362</epage><pages>7354-7362</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>We propose a novel competitive mechanism involving the dissolved oxygen (O2) between zirconium-based porphyrinic metal–organic framework nanoparticles (NMOFs) and luminol into a ratiometric electrochemiluminescence (ECL) biosensing interface. Zinc tetrakis(carboxyphenyl)-porphyrin (ZnTCPP) in NMOFs as electron media reduce O2 into reactive oxygen species (ROS) and produce singlet oxygen (1O2), resulting in cathodic ECL. Meanwhile, ROS also react with the luminol anion radical and amplify the anodic ECL emission. Based on the competitive-mechanism-driven ECL process, taking the detection of polynucleotide kinase (PNK) as example, with assembling DNA-functionalized NMOFs on the sensing interface, a lower detection limit of 6.5 × 10–5 U mL–1 and broader linear relationship range from 0.0002 to 10 U mL–1 were obtained compared with that of single-signal-driven ECL sensors. This proposed MOFs-luminol competitive ECL mechanism involving dissolved O2 may provide a new pathway for further research of a green and highly sensitive ECL biosensing system.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>32319281</pmid><doi>10.1021/acs.analchem.0c01262</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-7159-5166</orcidid><orcidid>https://orcid.org/0000-0002-0951-4222</orcidid><orcidid>https://orcid.org/0000-0002-0041-126X</orcidid><orcidid>https://orcid.org/0000-0002-0035-3821</orcidid><orcidid>https://orcid.org/0000-0002-7267-2952</orcidid></addata></record> |
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| subjects | Biosensing Techniques Biosensors Chemistry Competition Deoxyribonucleic acid Dissolved oxygen DNA Electrochemical Techniques Electrochemiluminescence Enzyme Inhibitors - pharmacology HeLa Cells Humans Kinases Luminescent Measurements Luminol Metal-organic frameworks Metal-Organic Frameworks - chemical synthesis Metal-Organic Frameworks - chemistry Metalloporphyrins Molecular Structure Nanoparticles Nanoparticles - chemistry Oxygen Particle Size Polynucleotide 5'-Hydroxyl-Kinase - analysis Polynucleotide 5'-Hydroxyl-Kinase - antagonists & inhibitors Polynucleotide 5'-Hydroxyl-Kinase - metabolism Polynucleotide kinase Reactive oxygen species Singlet oxygen Surface Properties Zirconium |
| title | Zirconium–Metalloporphyrin Frameworks–Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity |
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