Point mutagenesis in mouse reveals contrasting pathogenetic effects between MEN2B‐ and Hirschsprung disease‐associated missense mutations of the RET gene

Missense mutations of the RET gene have been identified in both multiple endocrine neoplasia (MEN) type 2A/B and Hirschsprung disease (HSCR: congenital absence of the enteric nervous system, ENS). Current consensus holds that MEN2A/B and HSCR are caused by activating and inactivating RET mutations,...

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Veröffentlicht in:Development, growth & differentiation growth & differentiation, 2020-05, Vol.62 (4), p.214-222
Hauptverfasser: Nakatani, Taichi, Iwasaki, Mitsuhiro, Yamamichi, Atsuhiro, Yoshioka, Yuta, Uesaka, Toshihiro, Bitoh, Yuko, Maeda, Kosaku, Fukumoto, Takumi, Takemoto, Tatsuya, Enomoto, Hideki
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container_title Development, growth & differentiation
container_volume 62
creator Nakatani, Taichi
Iwasaki, Mitsuhiro
Yamamichi, Atsuhiro
Yoshioka, Yuta
Uesaka, Toshihiro
Bitoh, Yuko
Maeda, Kosaku
Fukumoto, Takumi
Takemoto, Tatsuya
Enomoto, Hideki
description Missense mutations of the RET gene have been identified in both multiple endocrine neoplasia (MEN) type 2A/B and Hirschsprung disease (HSCR: congenital absence of the enteric nervous system, ENS). Current consensus holds that MEN2A/B and HSCR are caused by activating and inactivating RET mutations, respectively. However, the biological significance of RET missense mutations in vivo has not been fully elucidated. In the present study, we introduced one MEN2B‐associated (M918T) and two HSCR‐associated (N394K and Y791F) RET missense mutations into the corresponding regions of the mouse Ret gene by genome editing (RetM919T, RetN396K and RetY792F) and performed histological examinations of Ret‐expressing tissues to understand the pathogenetic impact of each mutant in vivo. RetM919T/+ mice displayed MEN2B‐related phenotypes, including C‐cell hyperplasia and abnormal enlargement of the primary sympathetic ganglia. Similar sympathetic phenotype was observed in RetM919T/‐ mice, demonstrating a strong pathogenetic effect of the Ret M918T by a single‐allele expression. In contrast, no abnormality was found in the ENS of mice harboring the Ret N394K or Y791F mutation. Most surprisingly, single‐allele expression of RET N394K or Y791F was sufficient for normal ENS development, indicating that these RET mutants exert largely physiological function in vivo. This study reveals contrasting pathogenetic effects between MEN2B‐ and HSCR‐associated RET missense mutations, and suggests that some of HSCR‐associated RET missense mutations are by themselves neither inactivating nor pathogenetic and require involvement of other gene mutations for disease expressivity. Biological significance of RET missense mutations has largely been unclear. In this study, MEN2B‐ and Hirschsprung disease‐associated RET missense mutations were introduced into the mouse Ret locus by CRISPR/Cas9‐mediated genome editing. Although strong pathogenetic effects were observed in mice carrying the MEN2B‐associated mutation, no pathogenicity was observed in mice harboring HSCR‐associated mutations.
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Current consensus holds that MEN2A/B and HSCR are caused by activating and inactivating RET mutations, respectively. However, the biological significance of RET missense mutations in vivo has not been fully elucidated. In the present study, we introduced one MEN2B‐associated (M918T) and two HSCR‐associated (N394K and Y791F) RET missense mutations into the corresponding regions of the mouse Ret gene by genome editing (RetM919T, RetN396K and RetY792F) and performed histological examinations of Ret‐expressing tissues to understand the pathogenetic impact of each mutant in vivo. RetM919T/+ mice displayed MEN2B‐related phenotypes, including C‐cell hyperplasia and abnormal enlargement of the primary sympathetic ganglia. Similar sympathetic phenotype was observed in RetM919T/‐ mice, demonstrating a strong pathogenetic effect of the Ret M918T by a single‐allele expression. In contrast, no abnormality was found in the ENS of mice harboring the Ret N394K or Y791F mutation. Most surprisingly, single‐allele expression of RET N394K or Y791F was sufficient for normal ENS development, indicating that these RET mutants exert largely physiological function in vivo. This study reveals contrasting pathogenetic effects between MEN2B‐ and HSCR‐associated RET missense mutations, and suggests that some of HSCR‐associated RET missense mutations are by themselves neither inactivating nor pathogenetic and require involvement of other gene mutations for disease expressivity. Biological significance of RET missense mutations has largely been unclear. In this study, MEN2B‐ and Hirschsprung disease‐associated RET missense mutations were introduced into the mouse Ret locus by CRISPR/Cas9‐mediated genome editing. 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Current consensus holds that MEN2A/B and HSCR are caused by activating and inactivating RET mutations, respectively. However, the biological significance of RET missense mutations in vivo has not been fully elucidated. In the present study, we introduced one MEN2B‐associated (M918T) and two HSCR‐associated (N394K and Y791F) RET missense mutations into the corresponding regions of the mouse Ret gene by genome editing (RetM919T, RetN396K and RetY792F) and performed histological examinations of Ret‐expressing tissues to understand the pathogenetic impact of each mutant in vivo. RetM919T/+ mice displayed MEN2B‐related phenotypes, including C‐cell hyperplasia and abnormal enlargement of the primary sympathetic ganglia. Similar sympathetic phenotype was observed in RetM919T/‐ mice, demonstrating a strong pathogenetic effect of the Ret M918T by a single‐allele expression. In contrast, no abnormality was found in the ENS of mice harboring the Ret N394K or Y791F mutation. Most surprisingly, single‐allele expression of RET N394K or Y791F was sufficient for normal ENS development, indicating that these RET mutants exert largely physiological function in vivo. This study reveals contrasting pathogenetic effects between MEN2B‐ and HSCR‐associated RET missense mutations, and suggests that some of HSCR‐associated RET missense mutations are by themselves neither inactivating nor pathogenetic and require involvement of other gene mutations for disease expressivity. Biological significance of RET missense mutations has largely been unclear. In this study, MEN2B‐ and Hirschsprung disease‐associated RET missense mutations were introduced into the mouse Ret locus by CRISPR/Cas9‐mediated genome editing. 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source Freely Accessible Japanese Titles (ERDB Project); Wiley-Blackwell Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Wiley Open Access
subjects Alleles
Animals
Enteric nervous system
Female
gene mutation
genome editing
Genomes
Hirschsprung disease
Hirschsprung Disease - genetics
Hirschsprung's disease
Hyperplasia
Mice
Mice, Inbred C57BL
Mice, Inbred ICR
Missense mutation
Multiple endocrine neoplasia
Multiple Endocrine Neoplasia Type 2b - genetics
Mutagenesis
Mutants
Mutation
Mutation, Missense
Phenotypes
Point Mutation
Proto-Oncogene Proteins c-ret - genetics
RET
Ret protein
Sympathetic ganglia
title Point mutagenesis in mouse reveals contrasting pathogenetic effects between MEN2B‐ and Hirschsprung disease‐associated missense mutations of the RET gene
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