The in vitro evaluation of anti-chlamydial and cytotoxic properties of dermaseptin S4 and derivatives: peptides from amphibian skin
Chlamydia trachomatis is an obligate intracellular bacterium responsible for a number of health problems, including sexually transmitted infection in humans. Efforts were made for the search of alternative therapies. Accordingly, the present study was undertaken to perform a systemic in vitro invest...
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| Veröffentlicht in: | Medicinal chemistry research 2013-12, Vol.22 (12), p.6096-6104 |
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| creator | Bergaoui, Ines Zaïri, Amira Gharsallah, Houda Aouni, Mahjoub Hammami, Adnene Hani, Khaled Selmi, Boulbaba |
| description | Chlamydia trachomatis
is an obligate intracellular bacterium responsible for a number of health problems, including sexually transmitted infection in humans. Efforts were made for the search of alternative therapies. Accordingly, the present study was undertaken to perform a systemic in vitro investigation on the anti-chlamydial potential of cationic peptides from frog’s skin, namely dermaseptin S
4
(S
4
) and its derivatives. Several strains of
Chlamydia trachomatis
serovar E were used to detect the antimicrobial activity of the new compounds. The infections tests and the toxic effects of the new compounds were determined using McCoy cells monolayers. Our data show that S
4
exhibited a potent anti-chlamydial activity and found that these peptides blocked infection of McCoy cells and reduced the numbers of inclusion-forming units (81 %) after 48 h at low concentration (5 μg/ml). Besides, the finding revealed that increasing the number of positive charges of the peptide resulted in a reduced cytotoxicity without affecting the antimicrobial effect. Among all peptides, the derivative K
4
K
20
S
4
was the more potent to inhibit
C. trachomatis
growth with 96 % reduction in the number of chlamydial inclusions compared with an untreated control infection and, therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases. |
| doi_str_mv | 10.1007/s00044-013-0601-9 |
| format | Article |
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is an obligate intracellular bacterium responsible for a number of health problems, including sexually transmitted infection in humans. Efforts were made for the search of alternative therapies. Accordingly, the present study was undertaken to perform a systemic in vitro investigation on the anti-chlamydial potential of cationic peptides from frog’s skin, namely dermaseptin S
4
(S
4
) and its derivatives. Several strains of
Chlamydia trachomatis
serovar E were used to detect the antimicrobial activity of the new compounds. The infections tests and the toxic effects of the new compounds were determined using McCoy cells monolayers. Our data show that S
4
exhibited a potent anti-chlamydial activity and found that these peptides blocked infection of McCoy cells and reduced the numbers of inclusion-forming units (81 %) after 48 h at low concentration (5 μg/ml). Besides, the finding revealed that increasing the number of positive charges of the peptide resulted in a reduced cytotoxicity without affecting the antimicrobial effect. Among all peptides, the derivative K
4
K
20
S
4
was the more potent to inhibit
C. trachomatis
growth with 96 % reduction in the number of chlamydial inclusions compared with an untreated control infection and, therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.</description><identifier>ISSN: 1054-2523</identifier><identifier>EISSN: 1554-8120</identifier><identifier>DOI: 10.1007/s00044-013-0601-9</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Antiinfectives and antibacterials ; Antimicrobial activity ; Antimicrobial agents ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Cationic peptides ; Cell Biology ; Chlamydia ; Chlamydia trachomatis ; Cytotoxicity ; Derivatives ; Inclusions ; Infections ; Infectious diseases ; McCoy cells ; Original Research ; Peptides ; Pharmacology/Toxicology ; Toxicity</subject><ispartof>Medicinal chemistry research, 2013-12, Vol.22 (12), p.6096-6104</ispartof><rights>Springer Science+Business Media New York 2013</rights><rights>Springer Science+Business Media New York 2013.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2749-d86b3067d1cbaae4876e7c3ef5e8688a5d2eef14e299000d34aa3e1ad6b42a053</citedby><cites>FETCH-LOGICAL-c2749-d86b3067d1cbaae4876e7c3ef5e8688a5d2eef14e299000d34aa3e1ad6b42a053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00044-013-0601-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00044-013-0601-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Bergaoui, Ines</creatorcontrib><creatorcontrib>Zaïri, Amira</creatorcontrib><creatorcontrib>Gharsallah, Houda</creatorcontrib><creatorcontrib>Aouni, Mahjoub</creatorcontrib><creatorcontrib>Hammami, Adnene</creatorcontrib><creatorcontrib>Hani, Khaled</creatorcontrib><creatorcontrib>Selmi, Boulbaba</creatorcontrib><title>The in vitro evaluation of anti-chlamydial and cytotoxic properties of dermaseptin S4 and derivatives: peptides from amphibian skin</title><title>Medicinal chemistry research</title><addtitle>Med Chem Res</addtitle><description>Chlamydia trachomatis
is an obligate intracellular bacterium responsible for a number of health problems, including sexually transmitted infection in humans. Efforts were made for the search of alternative therapies. Accordingly, the present study was undertaken to perform a systemic in vitro investigation on the anti-chlamydial potential of cationic peptides from frog’s skin, namely dermaseptin S
4
(S
4
) and its derivatives. Several strains of
Chlamydia trachomatis
serovar E were used to detect the antimicrobial activity of the new compounds. The infections tests and the toxic effects of the new compounds were determined using McCoy cells monolayers. Our data show that S
4
exhibited a potent anti-chlamydial activity and found that these peptides blocked infection of McCoy cells and reduced the numbers of inclusion-forming units (81 %) after 48 h at low concentration (5 μg/ml). Besides, the finding revealed that increasing the number of positive charges of the peptide resulted in a reduced cytotoxicity without affecting the antimicrobial effect. Among all peptides, the derivative K
4
K
20
S
4
was the more potent to inhibit
C. trachomatis
growth with 96 % reduction in the number of chlamydial inclusions compared with an untreated control infection and, therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.</description><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial activity</subject><subject>Antimicrobial agents</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cationic peptides</subject><subject>Cell Biology</subject><subject>Chlamydia</subject><subject>Chlamydia trachomatis</subject><subject>Cytotoxicity</subject><subject>Derivatives</subject><subject>Inclusions</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>McCoy cells</subject><subject>Original Research</subject><subject>Peptides</subject><subject>Pharmacology/Toxicology</subject><subject>Toxicity</subject><issn>1054-2523</issn><issn>1554-8120</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRSMEEqXwAewssQ6MH3mxQ4iXVIkFZW1N4gl1aeJgpxVd8-O4FIkVK4_tc6-tkyTnHC45QHEVAECpFLhMIQeeVgfJhGeZSksu4DDOEGeRCXmcnISwBJAFqGySfM0XxGzPNnb0jtEGV2screuZaxn2o02bxQq7rbG4invDmu3oRvdpGzZ4N5AfLYUda8h3GGgYY9eL-kHjkd3Esg2FazbsrkxkW-86ht2wsLXFnoV3258mRy2uAp39rtPk9f5ufvuYzp4fnm5vZmkjClWlpsxrCXlheFMjkiqLnIpGUptRmZclZkYQtVyRqKpow0iFKImjyWslEDI5TS72vfHrH2sKo166te_jk1ooULIquFSR4nuq8S4ET60evO3QbzUHvXOt9651dK13rnUVM2KfCZHt38j_Nf8f-gb7zIO_</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Bergaoui, Ines</creator><creator>Zaïri, Amira</creator><creator>Gharsallah, Houda</creator><creator>Aouni, Mahjoub</creator><creator>Hammami, Adnene</creator><creator>Hani, Khaled</creator><creator>Selmi, Boulbaba</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope></search><sort><creationdate>201312</creationdate><title>The in vitro evaluation of anti-chlamydial and cytotoxic properties of dermaseptin S4 and derivatives: peptides from amphibian skin</title><author>Bergaoui, Ines ; Zaïri, Amira ; Gharsallah, Houda ; Aouni, Mahjoub ; Hammami, Adnene ; Hani, Khaled ; Selmi, Boulbaba</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2749-d86b3067d1cbaae4876e7c3ef5e8688a5d2eef14e299000d34aa3e1ad6b42a053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial activity</topic><topic>Antimicrobial agents</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cationic peptides</topic><topic>Cell Biology</topic><topic>Chlamydia</topic><topic>Chlamydia trachomatis</topic><topic>Cytotoxicity</topic><topic>Derivatives</topic><topic>Inclusions</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>McCoy cells</topic><topic>Original Research</topic><topic>Peptides</topic><topic>Pharmacology/Toxicology</topic><topic>Toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bergaoui, Ines</creatorcontrib><creatorcontrib>Zaïri, Amira</creatorcontrib><creatorcontrib>Gharsallah, Houda</creatorcontrib><creatorcontrib>Aouni, Mahjoub</creatorcontrib><creatorcontrib>Hammami, Adnene</creatorcontrib><creatorcontrib>Hani, Khaled</creatorcontrib><creatorcontrib>Selmi, Boulbaba</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Medicinal chemistry research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bergaoui, Ines</au><au>Zaïri, Amira</au><au>Gharsallah, Houda</au><au>Aouni, Mahjoub</au><au>Hammami, Adnene</au><au>Hani, Khaled</au><au>Selmi, Boulbaba</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The in vitro evaluation of anti-chlamydial and cytotoxic properties of dermaseptin S4 and derivatives: peptides from amphibian skin</atitle><jtitle>Medicinal chemistry research</jtitle><stitle>Med Chem Res</stitle><date>2013-12</date><risdate>2013</risdate><volume>22</volume><issue>12</issue><spage>6096</spage><epage>6104</epage><pages>6096-6104</pages><issn>1054-2523</issn><eissn>1554-8120</eissn><abstract>Chlamydia trachomatis
is an obligate intracellular bacterium responsible for a number of health problems, including sexually transmitted infection in humans. Efforts were made for the search of alternative therapies. Accordingly, the present study was undertaken to perform a systemic in vitro investigation on the anti-chlamydial potential of cationic peptides from frog’s skin, namely dermaseptin S
4
(S
4
) and its derivatives. Several strains of
Chlamydia trachomatis
serovar E were used to detect the antimicrobial activity of the new compounds. The infections tests and the toxic effects of the new compounds were determined using McCoy cells monolayers. Our data show that S
4
exhibited a potent anti-chlamydial activity and found that these peptides blocked infection of McCoy cells and reduced the numbers of inclusion-forming units (81 %) after 48 h at low concentration (5 μg/ml). Besides, the finding revealed that increasing the number of positive charges of the peptide resulted in a reduced cytotoxicity without affecting the antimicrobial effect. Among all peptides, the derivative K
4
K
20
S
4
was the more potent to inhibit
C. trachomatis
growth with 96 % reduction in the number of chlamydial inclusions compared with an untreated control infection and, therefore, can be considered as potential agents for therapy of Chlamydia infectious diseases.</abstract><cop>New York</cop><pub>Springer US</pub><doi>10.1007/s00044-013-0601-9</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Medicinal chemistry research, 2013-12, Vol.22 (12), p.6096-6104 |
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| source | Springer Nature - Complete Springer Journals |
| subjects | Antiinfectives and antibacterials Antimicrobial activity Antimicrobial agents Biochemistry Biomedical and Life Sciences Biomedicine Cationic peptides Cell Biology Chlamydia Chlamydia trachomatis Cytotoxicity Derivatives Inclusions Infections Infectious diseases McCoy cells Original Research Peptides Pharmacology/Toxicology Toxicity |
| title | The in vitro evaluation of anti-chlamydial and cytotoxic properties of dermaseptin S4 and derivatives: peptides from amphibian skin |
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