Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples
Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The “micro-amount of virion enrichment technique” (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction w...
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| Veröffentlicht in: | Food and environmental virology 2020-06, Vol.12 (2), p.167-173 |
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| creator | Makino, Ryohei Yamazaki, Yasuko Nagao, Konomu Apego, Francis Victor Mekata, Hirohisa Yamazaki, Wataru |
| description | Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The “micro-amount of virion enrichment technique” (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction with sodium dodecyl benzenesulfonate (SDBS) for the specific and sensitive detection of avian influenza viruses (AIVs). AIV subtypes H3N2 and H4N2 were used to spike the surface of chicken breast meat samples. The modified MiVET protocol was tested by comparing it against three different homogenate preparation conditions, as well as in samples with added α-amylase and collagenase to digest inhibitors. The performance of the modified MiVET was evaluated by real-time RT-PCR assay targeting the matrix gene. Compared with conventional RNA extraction, the modified MiVET reproducibly concentrated AIVs in chicken meat samples with 100–1000-fold improvement by 60 s-hand homogenization. The 30 s- and 60 s-stomacher homogenizations resulted 100-fold and 10–100-fold improvement, respectively. The modified MiVET required |
| doi_str_mv | 10.1007/s12560-020-09425-1 |
| format | Article |
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The “micro-amount of virion enrichment technique” (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction with sodium dodecyl benzenesulfonate (SDBS) for the specific and sensitive detection of avian influenza viruses (AIVs). AIV subtypes H3N2 and H4N2 were used to spike the surface of chicken breast meat samples. The modified MiVET protocol was tested by comparing it against three different homogenate preparation conditions, as well as in samples with added α-amylase and collagenase to digest inhibitors. The performance of the modified MiVET was evaluated by real-time RT-PCR assay targeting the matrix gene. Compared with conventional RNA extraction, the modified MiVET reproducibly concentrated AIVs in chicken meat samples with 100–1000-fold improvement by 60 s-hand homogenization. The 30 s- and 60 s-stomacher homogenizations resulted 100-fold and 10–100-fold improvement, respectively. The modified MiVET required < 60 min from homogenate preparation to final RNA elution. Further, use of the modified MiVET also decreased the rate of false-negative results. The modified MiVET is effective for the rapid and highly sensitive detection of AIVs in chicken meat samples, and can be applied to quarantine and export inspection at airports and seaports.</description><identifier>ISSN: 1867-0334</identifier><identifier>EISSN: 1867-0342</identifier><identifier>DOI: 10.1007/s12560-020-09425-1</identifier><identifier>PMID: 32193766</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Airports ; Animals ; Avian flu ; Beads ; Biomedical and Life Sciences ; Biomedicine ; Chemistry/Food Science ; Chickens ; Collagen ; Collagenase ; Elution ; Enrichment ; Exports ; Food Microbiology - instrumentation ; Food Microbiology - methods ; Food Science ; Influenza ; Influenza A ; Influenza A virus - classification ; Influenza A virus - genetics ; Influenza A virus - isolation & purification ; Influenza A Virus, H3N2 Subtype - classification ; Influenza A Virus, H3N2 Subtype - genetics ; Influenza A Virus, H3N2 Subtype - isolation & purification ; Influenza in Birds - virology ; Inspection ; Meat ; Meat - virology ; Original Paper ; Polymerase chain reaction ; Poultry ; Poultry Diseases - virology ; Quarantine ; Ribonucleic acid ; RNA ; Virion - classification ; Virion - genetics ; Virion - isolation & purification ; Virions ; Virology ; Virology - instrumentation ; Virology - methods ; Viruses ; α-Amylase</subject><ispartof>Food and environmental virology, 2020-06, Vol.12 (2), p.167-173</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-696684f84dd4a98452adf062e88444b54746e4d1d6f786857860f7f298e9064d3</citedby><cites>FETCH-LOGICAL-c529t-696684f84dd4a98452adf062e88444b54746e4d1d6f786857860f7f298e9064d3</cites><orcidid>0000-0002-7874-8014</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12560-020-09425-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12560-020-09425-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32193766$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Makino, Ryohei</creatorcontrib><creatorcontrib>Yamazaki, Yasuko</creatorcontrib><creatorcontrib>Nagao, Konomu</creatorcontrib><creatorcontrib>Apego, Francis Victor</creatorcontrib><creatorcontrib>Mekata, Hirohisa</creatorcontrib><creatorcontrib>Yamazaki, Wataru</creatorcontrib><title>Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples</title><title>Food and environmental virology</title><addtitle>Food Environ Virol</addtitle><addtitle>Food Environ Virol</addtitle><description>Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The “micro-amount of virion enrichment technique” (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction with sodium dodecyl benzenesulfonate (SDBS) for the specific and sensitive detection of avian influenza viruses (AIVs). AIV subtypes H3N2 and H4N2 were used to spike the surface of chicken breast meat samples. The modified MiVET protocol was tested by comparing it against three different homogenate preparation conditions, as well as in samples with added α-amylase and collagenase to digest inhibitors. The performance of the modified MiVET was evaluated by real-time RT-PCR assay targeting the matrix gene. Compared with conventional RNA extraction, the modified MiVET reproducibly concentrated AIVs in chicken meat samples with 100–1000-fold improvement by 60 s-hand homogenization. The 30 s- and 60 s-stomacher homogenizations resulted 100-fold and 10–100-fold improvement, respectively. The modified MiVET required < 60 min from homogenate preparation to final RNA elution. Further, use of the modified MiVET also decreased the rate of false-negative results. The modified MiVET is effective for the rapid and highly sensitive detection of AIVs in chicken meat samples, and can be applied to quarantine and export inspection at airports and seaports.</description><subject>Airports</subject><subject>Animals</subject><subject>Avian flu</subject><subject>Beads</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Chemistry/Food Science</subject><subject>Chickens</subject><subject>Collagen</subject><subject>Collagenase</subject><subject>Elution</subject><subject>Enrichment</subject><subject>Exports</subject><subject>Food Microbiology - instrumentation</subject><subject>Food Microbiology - methods</subject><subject>Food Science</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A virus - classification</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza A Virus, H3N2 Subtype - classification</subject><subject>Influenza A Virus, H3N2 Subtype - genetics</subject><subject>Influenza A Virus, H3N2 Subtype - isolation & purification</subject><subject>Influenza in Birds - virology</subject><subject>Inspection</subject><subject>Meat</subject><subject>Meat - virology</subject><subject>Original Paper</subject><subject>Polymerase chain reaction</subject><subject>Poultry</subject><subject>Poultry Diseases - virology</subject><subject>Quarantine</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>Virion - classification</subject><subject>Virion - genetics</subject><subject>Virion - isolation & purification</subject><subject>Virions</subject><subject>Virology</subject><subject>Virology - instrumentation</subject><subject>Virology - methods</subject><subject>Viruses</subject><subject>α-Amylase</subject><issn>1867-0334</issn><issn>1867-0342</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9OGzEQxq2qiNCUF-ihstRLe1jwv_V6j1FIAYmIA2mulrM7bgxZ79beRSqPwtPWSwi9cbDH8nzzG818CH2h5IwSUpxHynJJMsLSKQXLM_oBnVAli4xwwT6-vbmYoE8x3hMiOcv5MZpwRkteSHmCnmddt3OV6V3rcWux8fi66UL7CDVeuiq0mWnawfdjbu3CqFr44KptA-lzBdXWuz8D4O9Lt16sfmDbBtxvAV9AD9UBOnt0I9fb3QD-yeDZiBoidh7fde4htZpvXfUAHi_B9PjONN0O4md0ZM0uwulrnKJfPxer-VV2c3t5PZ_dZFXOyj6TpZRKWCXqWphSiZyZ2hLJQCkhxCYXhZAgalpLWyip8nQRW1hWKiiJFDWfom97bho7jRJ7fd8OwaeWmgnCKc1JClPE9qq0kxgDWN0F15jwV1OiRzv03g6d7NAvdmiair6-oodNA_VbyWH_ScD3gphS_jeE_73fwf4DdVKUlA</recordid><startdate>20200601</startdate><enddate>20200601</enddate><creator>Makino, Ryohei</creator><creator>Yamazaki, Yasuko</creator><creator>Nagao, Konomu</creator><creator>Apego, Francis Victor</creator><creator>Mekata, Hirohisa</creator><creator>Yamazaki, Wataru</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-7874-8014</orcidid></search><sort><creationdate>20200601</creationdate><title>Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples</title><author>Makino, Ryohei ; Yamazaki, Yasuko ; Nagao, Konomu ; Apego, Francis Victor ; Mekata, Hirohisa ; Yamazaki, Wataru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-696684f84dd4a98452adf062e88444b54746e4d1d6f786857860f7f298e9064d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Airports</topic><topic>Animals</topic><topic>Avian flu</topic><topic>Beads</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Chemistry/Food Science</topic><topic>Chickens</topic><topic>Collagen</topic><topic>Collagenase</topic><topic>Elution</topic><topic>Enrichment</topic><topic>Exports</topic><topic>Food Microbiology - instrumentation</topic><topic>Food Microbiology - methods</topic><topic>Food Science</topic><topic>Influenza</topic><topic>Influenza A</topic><topic>Influenza A virus - classification</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza A Virus, H3N2 Subtype - classification</topic><topic>Influenza A Virus, H3N2 Subtype - genetics</topic><topic>Influenza A Virus, H3N2 Subtype - isolation & purification</topic><topic>Influenza in Birds - virology</topic><topic>Inspection</topic><topic>Meat</topic><topic>Meat - virology</topic><topic>Original Paper</topic><topic>Polymerase chain reaction</topic><topic>Poultry</topic><topic>Poultry Diseases - virology</topic><topic>Quarantine</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>Virion - classification</topic><topic>Virion - genetics</topic><topic>Virion - isolation & purification</topic><topic>Virions</topic><topic>Virology</topic><topic>Virology - instrumentation</topic><topic>Virology - methods</topic><topic>Viruses</topic><topic>α-Amylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Makino, Ryohei</creatorcontrib><creatorcontrib>Yamazaki, Yasuko</creatorcontrib><creatorcontrib>Nagao, Konomu</creatorcontrib><creatorcontrib>Apego, Francis Victor</creatorcontrib><creatorcontrib>Mekata, Hirohisa</creatorcontrib><creatorcontrib>Yamazaki, Wataru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Food and environmental virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Makino, Ryohei</au><au>Yamazaki, Yasuko</au><au>Nagao, Konomu</au><au>Apego, Francis Victor</au><au>Mekata, Hirohisa</au><au>Yamazaki, Wataru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples</atitle><jtitle>Food and environmental virology</jtitle><stitle>Food Environ Virol</stitle><addtitle>Food Environ Virol</addtitle><date>2020-06-01</date><risdate>2020</risdate><volume>12</volume><issue>2</issue><spage>167</spage><epage>173</epage><pages>167-173</pages><issn>1867-0334</issn><eissn>1867-0342</eissn><abstract>Highly sensitive detection of pathogens is effective for screening meat during quarantine inspection and export. The “micro-amount of virion enrichment technique” (MiVET) was recently developed, which is a new method combining virus concentration with immunomagnetic beads and simple RNA extraction with sodium dodecyl benzenesulfonate (SDBS) for the specific and sensitive detection of avian influenza viruses (AIVs). AIV subtypes H3N2 and H4N2 were used to spike the surface of chicken breast meat samples. The modified MiVET protocol was tested by comparing it against three different homogenate preparation conditions, as well as in samples with added α-amylase and collagenase to digest inhibitors. The performance of the modified MiVET was evaluated by real-time RT-PCR assay targeting the matrix gene. Compared with conventional RNA extraction, the modified MiVET reproducibly concentrated AIVs in chicken meat samples with 100–1000-fold improvement by 60 s-hand homogenization. The 30 s- and 60 s-stomacher homogenizations resulted 100-fold and 10–100-fold improvement, respectively. The modified MiVET required < 60 min from homogenate preparation to final RNA elution. Further, use of the modified MiVET also decreased the rate of false-negative results. The modified MiVET is effective for the rapid and highly sensitive detection of AIVs in chicken meat samples, and can be applied to quarantine and export inspection at airports and seaports.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32193766</pmid><doi>10.1007/s12560-020-09425-1</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-7874-8014</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Airports Animals Avian flu Beads Biomedical and Life Sciences Biomedicine Chemistry/Food Science Chickens Collagen Collagenase Elution Enrichment Exports Food Microbiology - instrumentation Food Microbiology - methods Food Science Influenza Influenza A Influenza A virus - classification Influenza A virus - genetics Influenza A virus - isolation & purification Influenza A Virus, H3N2 Subtype - classification Influenza A Virus, H3N2 Subtype - genetics Influenza A Virus, H3N2 Subtype - isolation & purification Influenza in Birds - virology Inspection Meat Meat - virology Original Paper Polymerase chain reaction Poultry Poultry Diseases - virology Quarantine Ribonucleic acid RNA Virion - classification Virion - genetics Virion - isolation & purification Virions Virology Virology - instrumentation Virology - methods Viruses α-Amylase |
| title | Application of an Improved Micro-amount of Virion Enrichment Technique (MiVET) for the Detection of Avian Influenza A Virus in Spiked Chicken Meat Samples |
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