Effects of a Reserve Protein on Spodoptera frugiperda Development: A Biochemical and Molecular Approach to the Entomotoxic Mechanism

Talisin is a storage protein from Talisia esculenta seeds that presents lectin-like and peptidase inhibitor properties. These characteristics suggest that talisin plays a role in the plant defense process, making it a multifunctional protein. This work aimed to investigate the effects of chronic int...

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Veröffentlicht in:Molecules (Basel, Switzerland) Switzerland), 2020-05, Vol.25 (9), p.2195, Article 2195
Hauptverfasser: Oliveira, Carolina Turatti, Machado, Suzy Wider, Bezerra, Cezar da Silva, Cardoso, Marlon Henrique, Franco, Octavio Luiz, Silva, Carlos Peres, Alves, Demetrio Gomes, Rios, Cristina, Macedo, Maria Ligia R.
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container_title Molecules (Basel, Switzerland)
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creator Oliveira, Carolina Turatti
Machado, Suzy Wider
Bezerra, Cezar da Silva
Cardoso, Marlon Henrique
Franco, Octavio Luiz
Silva, Carlos Peres
Alves, Demetrio Gomes
Rios, Cristina
Macedo, Maria Ligia R.
description Talisin is a storage protein from Talisia esculenta seeds that presents lectin-like and peptidase inhibitor properties. These characteristics suggest that talisin plays a role in the plant defense process, making it a multifunctional protein. This work aimed to investigate the effects of chronic intake of talisin on fifth instar larvae of Spodoptera frugiperda, considered the main insect pest of maize and the cause of substantial economic losses in several other crops. The chronic intake of talisin presented antinutritional effects on the larvae, reducing their weight and prolonging the total development time of the insects. In addition, talisin-fed larvae also showed a significant reduction in the activity of trypsin-like enzymes. Midgut histology analysis of talisin-fed larvae showed alterations in the intestinal epithelium and rupture of the peritrophic membrane, possibly causing an increase of aminopeptidase activity in the midgut lumen. Talisin also proved to be resistant to degradation by the digestive enzymes of S. frugiperda. The transcription profile of trypsin, chymotrypsin and aminopeptidase genes was also analyzed through qPCR technique. Talisin intake resulted in differential expression of at least two genes from each of these classes of enzymes. Molecular docking studies indicated a higher affinity of talisin for the less expressed enzymes.
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These characteristics suggest that talisin plays a role in the plant defense process, making it a multifunctional protein. This work aimed to investigate the effects of chronic intake of talisin on fifth instar larvae of Spodoptera frugiperda, considered the main insect pest of maize and the cause of substantial economic losses in several other crops. The chronic intake of talisin presented antinutritional effects on the larvae, reducing their weight and prolonging the total development time of the insects. In addition, talisin-fed larvae also showed a significant reduction in the activity of trypsin-like enzymes. Midgut histology analysis of talisin-fed larvae showed alterations in the intestinal epithelium and rupture of the peritrophic membrane, possibly causing an increase of aminopeptidase activity in the midgut lumen. Talisin also proved to be resistant to degradation by the digestive enzymes of S. frugiperda. 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subjects Aminopeptidase
Animals
Biochemistry & Molecular Biology
bioinsecticides
Chemistry
Chemistry, Multidisciplinary
Chymotrypsin
Crops
Defense mechanisms
Digestive enzymes
Economic impact
enzyme activity
Enzymes
Epithelium
Food
Gene Expression Regulation, Enzymologic - drug effects
Genes
Histology
Hydrogen bonds
insect gut
Insect Proteins - biosynthesis
Insect Proteins - genetics
Insects
Intestinal Mucosa - enzymology
Intestine
Larva - genetics
Larva - growth & development
Larvae
lectin properties
Life Sciences & Biomedicine
Midgut
Molecular docking
multifunctional protein
Peptidase
Peptide Hydrolases - biosynthesis
Peptide Hydrolases - genetics
Peritrophic membrane
Physical Sciences
Proteins
Receptors, Cell Surface
Science & Technology
Seeds
Spodoptera - genetics
Spodoptera - growth & development
Spodoptera frugiperda
Transcription
Trypsin
Weight reduction
title Effects of a Reserve Protein on Spodoptera frugiperda Development: A Biochemical and Molecular Approach to the Entomotoxic Mechanism
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