Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm

A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, “Rocha” dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as t...

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Veröffentlicht in:Sustainability 2019-10, Vol.11 (19), p.5340
Hauptverfasser: Queiroz, Álvaro, Bagoin Guimarães, Joana, Sánchez, Claudia, Simões, Fernanda, Maia de Sousa, Rui, Viegas, Wanda, Veloso, Maria Manuela
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container_end_page
container_issue 19
container_start_page 5340
container_title Sustainability
container_volume 11
creator Queiroz, Álvaro
Bagoin Guimarães, Joana
Sánchez, Claudia
Simões, Fernanda
Maia de Sousa, Rui
Viegas, Wanda
Veloso, Maria Manuela
description A rich heritage of traditional pear varieties is kept in national Portuguese collections. Out of these varieties, “Rocha” dominates national pear production. Although a noticeable phenotypic variation among clones of this variety has been reported, little is known about its genetic variability, as to date molecular studies have been performed on a single “Rocha” clone. Eleven Simple Sequence Repeats (SSR) markers were used to assess the genetic diversity of 130 local cultivars, 80 of them being “Rocha” clones. The results allowed the differentiation of 75 genotypes of which 29 are “Rocha”. Three synonyms groups and four homonymous groups of other local varieties were confirmed. A Bayesian model-based clustering approach identified two distinct clusters. Using flow cytometry, six cultivars were found to be triploids. These results show high genetic variability among “Rocha” clones. In conclusion, there is a need for different “Rocha” clones to be preserved to enable the correct selection of the multiplication material.
doi_str_mv 10.3390/su11195340
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source MDPI - Multidisciplinary Digital Publishing Institute; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
subjects Bayesian analysis
Cloning
Clustering
Cultivars
Data analysis
Deoxyribonucleic acid
DNA
Flow cytometry
Fruits
Genetic diversity
Genetic variability
Genomes
Genotypes
Germplasm
Multiplication
Phenotypic variations
Polyploidy
Simple sequence repeats
Software
Sustainability
title Genetic Diversity and Structure of the Portuguese Pear (Pyrus communis L.) Germplasm
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