A RuO2 Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution

A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fl...

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Veröffentlicht in:Analytical Sciences 2020/04/10, Vol.36(4), pp.397-400
Hauptverfasser: KITAMURA, Yusuke, TANIGUCHI, Takaaki, TSUTSUMI, Miwako, NURDIWIJAYANTO, Leanddas, MATSUO, Tomoya, KATSUDA, Yousuke, IHARA, Toshihiro
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container_end_page 400
container_issue 4
container_start_page 397
container_title Analytical Sciences
container_volume 36
creator KITAMURA, Yusuke
TANIGUCHI, Takaaki
TSUTSUMI, Miwako
NURDIWIJAYANTO, Leanddas
MATSUO, Tomoya
KATSUDA, Yousuke
IHARA, Toshihiro
description A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fluorescence recovery was higher than that for graphene oxide, which was known as a quencher-free platform for the detection of nucleic acids in a homogeneous solution.
doi_str_mv 10.2116/analsci.20C004
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Springer Nature - Complete Springer Journals; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Free Full-Text Journals in Chemistry
subjects Analytical Chemistry
Chemistry
Complementary DNA
Fluorescence
Fluorescent dyes
Fluorescent indicators
Graphene
homogeneous assay
Nanosheets
Nucleic acids
Nucleic acids sensor
quencher-free analytical platform
Rapid Communications
Recovery
RuO2 nanosheet
Ruthenium oxide
title A RuO2 Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution
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