A RuO2 Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution
A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fl...
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Veröffentlicht in: | Analytical Sciences 2020/04/10, Vol.36(4), pp.397-400 |
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creator | KITAMURA, Yusuke TANIGUCHI, Takaaki TSUTSUMI, Miwako NURDIWIJAYANTO, Leanddas MATSUO, Tomoya KATSUDA, Yousuke IHARA, Toshihiro |
description | A fluorescent dye-labeled DNA probe was adsorbed and quenched on the monolayer of RuO2 nanosheets. Significant fluorescent recovery was observed upon the addition of complementary DNA due to desorption of the probe from the surface of the RuO2 nanosheet through duplex formation. The efficiency of fluorescence recovery was higher than that for graphene oxide, which was known as a quencher-free platform for the detection of nucleic acids in a homogeneous solution. |
doi_str_mv | 10.2116/analsci.20C004 |
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subjects | Analytical Chemistry Chemistry Complementary DNA Fluorescence Fluorescent dyes Fluorescent indicators Graphene homogeneous assay Nanosheets Nucleic acids Nucleic acids sensor quencher-free analytical platform Rapid Communications Recovery RuO2 nanosheet Ruthenium oxide |
title | A RuO2 Nanosheet as a Novel Quencher-free Platform for the Detection of Nucleic Acids in a Homogeneous Solution |
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