Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq
Leaf morphology varies significantly among Brassica juncea . However, little is known regarding the causal gene that controls lobed leaf shape in B. juncea . In our study, the BjRCO gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in B. juncea via BSR-seq....
Gespeichert in:
| Veröffentlicht in: | Molecular breeding 2020-04, Vol.40 (4), Article 42 |
|---|---|
| Hauptverfasser: | , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 4 |
| container_start_page | |
| container_title | Molecular breeding |
| container_volume | 40 |
| creator | Heng, Shuangping Huang, Hao Cui, Mengdi Liu, Mingfang Lv, Qing Hu, Peiyao Ren, Sujing Li, Xiao Fu, Tingdong Wan, Zhengjie |
| description | Leaf morphology varies significantly among
Brassica juncea
. However, little is known regarding the causal gene that controls lobed leaf shape in
B. juncea
. In our study, the
BjRCO
gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in
B. juncea
via BSR-seq. Blast analysis suggested that the BjRCO encodes a HD-Zip I transcription factor, which may serve as a positive regulator of lobed leaf formation in leaf mustard. Interestingly, the lobed leaf genes reported in the related species of
Brassica
(
B. rapa
,
B. napus
, and
B. juncea
) were located in A10. Phylogenetic analysis showed that BjRCO was also homologous to the lobed leaf gene
BoLl
reported on chromosome 9 in
B. oleracea
. In addition, qRT-PCR results showed that the expression levels of the
BjRCO
gene were higher expressed in the inner small lobed leaves than in the little sawtooth-like leaves (LSL). These results of this study indicate that the
BjRCO
gene is related to lobed leaf formation in
B. juncea
. These findings help to establish a solid foundation for future research investigating the molecular mechanism governing lobed leaf formation in
B. juncea
. |
| doi_str_mv | 10.1007/s11032-020-01119-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2390053220</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2390053220</sourcerecordid><originalsourceid>FETCH-LOGICAL-c319t-6981b0015c669961cd9170a4f92183306b49e39fc5a6a60a131abcbdb649b2813</originalsourceid><addsrcrecordid>eNp9kE1LAzEURYMoWKt_wFXAdfS9pM1Mlm3xC4qFouuQyWTa1HGmTWYU_73REdy5endxzn1wCblEuEaA7CYiguAMODBARMWyIzLCacaZyvL8OGWRAxPZRJySsxh3kCQl5YjEtdn7kvrSNZ2vvDWdbxvaVrTbOjrfrRcrunGNoybG1nrTuZJ--G5L67ZIsXbm3UXqGzoPiUg63fWNdYa-e0OLvn5NUHSb4Dam6ej6acaiO5yTk8rU0V383jF5ubt9Xjyw5er-cTFbMitQdUyqHAsAnFoplZJoS4UZmEmlOOZCgCwmyglV2amRRoJBgaawRVnIiSp4jmJMrobefWgPvYud3rV9aNJLzYUCmArOIVF8oGxoYwyu0vvg30z41Aj6e1w9jKvTuPpnXJ0lSQxSTHCzceGv-h_rC1W4e78</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2390053220</pqid></control><display><type>article</type><title>Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq</title><source>SpringerLink Journals - AutoHoldings</source><creator>Heng, Shuangping ; Huang, Hao ; Cui, Mengdi ; Liu, Mingfang ; Lv, Qing ; Hu, Peiyao ; Ren, Sujing ; Li, Xiao ; Fu, Tingdong ; Wan, Zhengjie</creator><creatorcontrib>Heng, Shuangping ; Huang, Hao ; Cui, Mengdi ; Liu, Mingfang ; Lv, Qing ; Hu, Peiyao ; Ren, Sujing ; Li, Xiao ; Fu, Tingdong ; Wan, Zhengjie</creatorcontrib><description>Leaf morphology varies significantly among
Brassica juncea
. However, little is known regarding the causal gene that controls lobed leaf shape in
B. juncea
. In our study, the
BjRCO
gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in
B. juncea
via BSR-seq. Blast analysis suggested that the BjRCO encodes a HD-Zip I transcription factor, which may serve as a positive regulator of lobed leaf formation in leaf mustard. Interestingly, the lobed leaf genes reported in the related species of
Brassica
(
B. rapa
,
B. napus
, and
B. juncea
) were located in A10. Phylogenetic analysis showed that BjRCO was also homologous to the lobed leaf gene
BoLl
reported on chromosome 9 in
B. oleracea
. In addition, qRT-PCR results showed that the expression levels of the
BjRCO
gene were higher expressed in the inner small lobed leaves than in the little sawtooth-like leaves (LSL). These results of this study indicate that the
BjRCO
gene is related to lobed leaf formation in
B. juncea
. These findings help to establish a solid foundation for future research investigating the molecular mechanism governing lobed leaf formation in
B. juncea
.</description><identifier>ISSN: 1380-3743</identifier><identifier>EISSN: 1572-9788</identifier><identifier>DOI: 10.1007/s11032-020-01119-7</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Brassica ; Brassica juncea ; Chromosome 9 ; Chromosomes ; Gene expression ; Homology ; Leaves ; Life Sciences ; Molecular biology ; Morphology ; Mustard ; Phylogeny ; Plant biology ; Plant Genetics and Genomics ; Plant Pathology ; Plant Physiology ; Plant Sciences ; Ribonucleic acid ; RNA</subject><ispartof>Molecular breeding, 2020-04, Vol.40 (4), Article 42</ispartof><rights>Springer Nature B.V. 2020</rights><rights>Springer Nature B.V. 2020.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c319t-6981b0015c669961cd9170a4f92183306b49e39fc5a6a60a131abcbdb649b2813</citedby><cites>FETCH-LOGICAL-c319t-6981b0015c669961cd9170a4f92183306b49e39fc5a6a60a131abcbdb649b2813</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11032-020-01119-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11032-020-01119-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Heng, Shuangping</creatorcontrib><creatorcontrib>Huang, Hao</creatorcontrib><creatorcontrib>Cui, Mengdi</creatorcontrib><creatorcontrib>Liu, Mingfang</creatorcontrib><creatorcontrib>Lv, Qing</creatorcontrib><creatorcontrib>Hu, Peiyao</creatorcontrib><creatorcontrib>Ren, Sujing</creatorcontrib><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Fu, Tingdong</creatorcontrib><creatorcontrib>Wan, Zhengjie</creatorcontrib><title>Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq</title><title>Molecular breeding</title><addtitle>Mol Breeding</addtitle><description>Leaf morphology varies significantly among
Brassica juncea
. However, little is known regarding the causal gene that controls lobed leaf shape in
B. juncea
. In our study, the
BjRCO
gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in
B. juncea
via BSR-seq. Blast analysis suggested that the BjRCO encodes a HD-Zip I transcription factor, which may serve as a positive regulator of lobed leaf formation in leaf mustard. Interestingly, the lobed leaf genes reported in the related species of
Brassica
(
B. rapa
,
B. napus
, and
B. juncea
) were located in A10. Phylogenetic analysis showed that BjRCO was also homologous to the lobed leaf gene
BoLl
reported on chromosome 9 in
B. oleracea
. In addition, qRT-PCR results showed that the expression levels of the
BjRCO
gene were higher expressed in the inner small lobed leaves than in the little sawtooth-like leaves (LSL). These results of this study indicate that the
BjRCO
gene is related to lobed leaf formation in
B. juncea
. These findings help to establish a solid foundation for future research investigating the molecular mechanism governing lobed leaf formation in
B. juncea
.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Brassica</subject><subject>Brassica juncea</subject><subject>Chromosome 9</subject><subject>Chromosomes</subject><subject>Gene expression</subject><subject>Homology</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Molecular biology</subject><subject>Morphology</subject><subject>Mustard</subject><subject>Phylogeny</subject><subject>Plant biology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Pathology</subject><subject>Plant Physiology</subject><subject>Plant Sciences</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><issn>1380-3743</issn><issn>1572-9788</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kE1LAzEURYMoWKt_wFXAdfS9pM1Mlm3xC4qFouuQyWTa1HGmTWYU_73REdy5endxzn1wCblEuEaA7CYiguAMODBARMWyIzLCacaZyvL8OGWRAxPZRJySsxh3kCQl5YjEtdn7kvrSNZ2vvDWdbxvaVrTbOjrfrRcrunGNoybG1nrTuZJ--G5L67ZIsXbm3UXqGzoPiUg63fWNdYa-e0OLvn5NUHSb4Dam6ej6acaiO5yTk8rU0V383jF5ubt9Xjyw5er-cTFbMitQdUyqHAsAnFoplZJoS4UZmEmlOOZCgCwmyglV2amRRoJBgaawRVnIiSp4jmJMrobefWgPvYud3rV9aNJLzYUCmArOIVF8oGxoYwyu0vvg30z41Aj6e1w9jKvTuPpnXJ0lSQxSTHCzceGv-h_rC1W4e78</recordid><startdate>20200401</startdate><enddate>20200401</enddate><creator>Heng, Shuangping</creator><creator>Huang, Hao</creator><creator>Cui, Mengdi</creator><creator>Liu, Mingfang</creator><creator>Lv, Qing</creator><creator>Hu, Peiyao</creator><creator>Ren, Sujing</creator><creator>Li, Xiao</creator><creator>Fu, Tingdong</creator><creator>Wan, Zhengjie</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M0K</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope></search><sort><creationdate>20200401</creationdate><title>Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq</title><author>Heng, Shuangping ; Huang, Hao ; Cui, Mengdi ; Liu, Mingfang ; Lv, Qing ; Hu, Peiyao ; Ren, Sujing ; Li, Xiao ; Fu, Tingdong ; Wan, Zhengjie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c319t-6981b0015c669961cd9170a4f92183306b49e39fc5a6a60a131abcbdb649b2813</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Brassica</topic><topic>Brassica juncea</topic><topic>Chromosome 9</topic><topic>Chromosomes</topic><topic>Gene expression</topic><topic>Homology</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Molecular biology</topic><topic>Morphology</topic><topic>Mustard</topic><topic>Phylogeny</topic><topic>Plant biology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Pathology</topic><topic>Plant Physiology</topic><topic>Plant Sciences</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heng, Shuangping</creatorcontrib><creatorcontrib>Huang, Hao</creatorcontrib><creatorcontrib>Cui, Mengdi</creatorcontrib><creatorcontrib>Liu, Mingfang</creatorcontrib><creatorcontrib>Lv, Qing</creatorcontrib><creatorcontrib>Hu, Peiyao</creatorcontrib><creatorcontrib>Ren, Sujing</creatorcontrib><creatorcontrib>Li, Xiao</creatorcontrib><creatorcontrib>Fu, Tingdong</creatorcontrib><creatorcontrib>Wan, Zhengjie</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><jtitle>Molecular breeding</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heng, Shuangping</au><au>Huang, Hao</au><au>Cui, Mengdi</au><au>Liu, Mingfang</au><au>Lv, Qing</au><au>Hu, Peiyao</au><au>Ren, Sujing</au><au>Li, Xiao</au><au>Fu, Tingdong</au><au>Wan, Zhengjie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq</atitle><jtitle>Molecular breeding</jtitle><stitle>Mol Breeding</stitle><date>2020-04-01</date><risdate>2020</risdate><volume>40</volume><issue>4</issue><artnum>42</artnum><issn>1380-3743</issn><eissn>1572-9788</eissn><abstract>Leaf morphology varies significantly among
Brassica juncea
. However, little is known regarding the causal gene that controls lobed leaf shape in
B. juncea
. In our study, the
BjRCO
gene, which is associated with leaf morphological variation, was cloned from chromosome A10 in
B. juncea
via BSR-seq. Blast analysis suggested that the BjRCO encodes a HD-Zip I transcription factor, which may serve as a positive regulator of lobed leaf formation in leaf mustard. Interestingly, the lobed leaf genes reported in the related species of
Brassica
(
B. rapa
,
B. napus
, and
B. juncea
) were located in A10. Phylogenetic analysis showed that BjRCO was also homologous to the lobed leaf gene
BoLl
reported on chromosome 9 in
B. oleracea
. In addition, qRT-PCR results showed that the expression levels of the
BjRCO
gene were higher expressed in the inner small lobed leaves than in the little sawtooth-like leaves (LSL). These results of this study indicate that the
BjRCO
gene is related to lobed leaf formation in
B. juncea
. These findings help to establish a solid foundation for future research investigating the molecular mechanism governing lobed leaf formation in
B. juncea
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s11032-020-01119-7</doi></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1380-3743 |
| ispartof | Molecular breeding, 2020-04, Vol.40 (4), Article 42 |
| issn | 1380-3743 1572-9788 |
| language | eng |
| recordid | cdi_proquest_journals_2390053220 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Biotechnology Brassica Brassica juncea Chromosome 9 Chromosomes Gene expression Homology Leaves Life Sciences Molecular biology Morphology Mustard Phylogeny Plant biology Plant Genetics and Genomics Plant Pathology Plant Physiology Plant Sciences Ribonucleic acid RNA |
| title | Rapid identification of the BjRCO gene associated with lobed leaves in Brassica juncea via bulked segregant RNA-seq |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T01%3A22%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Rapid%20identification%20of%20the%20BjRCO%20gene%20associated%20with%20lobed%20leaves%20in%20Brassica%20juncea%20via%20bulked%20segregant%20RNA-seq&rft.jtitle=Molecular%20breeding&rft.au=Heng,%20Shuangping&rft.date=2020-04-01&rft.volume=40&rft.issue=4&rft.artnum=42&rft.issn=1380-3743&rft.eissn=1572-9788&rft_id=info:doi/10.1007/s11032-020-01119-7&rft_dat=%3Cproquest_cross%3E2390053220%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2390053220&rft_id=info:pmid/&rfr_iscdi=true |