Spruce and beech bark aqueous extracts: source of polyphenols, tannins and antioxidants correlated to in vitro antitumor potential on two different cell lines
The purpose of the present study was to determine the antioxidant capacity, the total phenol content, tannins, the cytotoxic and antiproliferative activity of some aqueous extracts obtained from spruce bark ( Picea abies L.) and beech bark ( Fagus sylvatica L.). In this regard, two types of extracts...
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| Veröffentlicht in: | Wood science and technology 2019-03, Vol.53 (2), p.313-333 |
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| creator | Coșarcă, Sanda-Liliana Moacă, Elena-Alina Tanase, Corneliu Muntean, Daniela Lucia Pavel, Ioana Zinuca Dehelean, Cristina Adriana |
| description | The purpose of the present study was to determine the antioxidant capacity, the total phenol content, tannins, the cytotoxic and antiproliferative activity of some aqueous extracts obtained from spruce bark (
Picea abies
L.) and beech bark (
Fagus sylvatica
L.). In this regard, two types of extracts were obtained from both species using different methods of extraction: classical water bath extraction and ultrasound-assisted extraction. The antioxidant activity (AOA) was determined by means of DPPH assay and the cytotoxic and antiproliferative effects were determined using Alamar Blue technique and scratch assay. The total phenol content was determined colorimetrically by means of the Folin–Ciocalteu method. In vitro antitumor activity was tested on two different cell lines: A375 (human melanoma) and A549 (lung carcinoma) because of their different origin and metastatic potential. In addition, a non-tumor cell line (HaCaT human keratinocytes) was used in order to evaluate the samples selectivity. The results indicated that the aqueous extracts obtained by classical extraction had a higher antioxidant activity compared to the extracts obtained by ultrasound-assisted extraction. Moreover, the AOA of
Picea abies
L. bark extracts proved to be superior to those obtained from
Fagus sylvatica
L. bark extracts. Regarding the effect on cancer cells, the samples elicited a dose-dependent cytotoxic effect on A375 human melanoma cells, while on A549 lung carcinoma cells, the extractive solutions stimulated tumor cells viability, an effect that was reduced by increasing the concentration of the samples. Furthermore, the extractive solutions proved to possess antiproliferative properties when tested at the highest concentration; results that could be correlated with the antioxidant effects. In the case of the non-tumor cell line, the samples did not display a cytotoxic activity and stimulated cells proliferation. |
| doi_str_mv | 10.1007/s00226-018-1071-5 |
| format | Article |
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Picea abies
L.) and beech bark (
Fagus sylvatica
L.). In this regard, two types of extracts were obtained from both species using different methods of extraction: classical water bath extraction and ultrasound-assisted extraction. The antioxidant activity (AOA) was determined by means of DPPH assay and the cytotoxic and antiproliferative effects were determined using Alamar Blue technique and scratch assay. The total phenol content was determined colorimetrically by means of the Folin–Ciocalteu method. In vitro antitumor activity was tested on two different cell lines: A375 (human melanoma) and A549 (lung carcinoma) because of their different origin and metastatic potential. In addition, a non-tumor cell line (HaCaT human keratinocytes) was used in order to evaluate the samples selectivity. The results indicated that the aqueous extracts obtained by classical extraction had a higher antioxidant activity compared to the extracts obtained by ultrasound-assisted extraction. Moreover, the AOA of
Picea abies
L. bark extracts proved to be superior to those obtained from
Fagus sylvatica
L. bark extracts. Regarding the effect on cancer cells, the samples elicited a dose-dependent cytotoxic effect on A375 human melanoma cells, while on A549 lung carcinoma cells, the extractive solutions stimulated tumor cells viability, an effect that was reduced by increasing the concentration of the samples. Furthermore, the extractive solutions proved to possess antiproliferative properties when tested at the highest concentration; results that could be correlated with the antioxidant effects. In the case of the non-tumor cell line, the samples did not display a cytotoxic activity and stimulated cells proliferation.</description><identifier>ISSN: 0043-7719</identifier><identifier>EISSN: 1432-5225</identifier><identifier>DOI: 10.1007/s00226-018-1071-5</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Anticancer properties ; Antioxidants ; Antitumor activity ; Bark ; Beech ; Biomedical and Life Sciences ; Biotechnology ; Cancer ; Cell proliferation ; Ceramics ; Colorimetry ; Composites ; Cytotoxicity ; Fagus sylvatica ; Glass ; In vitro methods and tests ; Keratinocytes ; Life Sciences ; Lung carcinoma ; Lungs ; Machines ; Manufacturing ; Melanoma ; Metastases ; Natural Materials ; Original ; Phenols ; Picea abies ; Plant extracts ; Polyphenols ; Processes ; Selectivity ; Tannins ; Tumor cell lines ; Tumor cells ; Ultrasonic imaging ; Ultrasound ; Viability ; Water baths ; Wood Science & Technology</subject><ispartof>Wood science and technology, 2019-03, Vol.53 (2), p.313-333</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-da22aa4cdb6d265ec9e144422aaff1a6fda423ad1aff10576dd611940b14f4933</citedby><cites>FETCH-LOGICAL-c353t-da22aa4cdb6d265ec9e144422aaff1a6fda423ad1aff10576dd611940b14f4933</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00226-018-1071-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00226-018-1071-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Coșarcă, Sanda-Liliana</creatorcontrib><creatorcontrib>Moacă, Elena-Alina</creatorcontrib><creatorcontrib>Tanase, Corneliu</creatorcontrib><creatorcontrib>Muntean, Daniela Lucia</creatorcontrib><creatorcontrib>Pavel, Ioana Zinuca</creatorcontrib><creatorcontrib>Dehelean, Cristina Adriana</creatorcontrib><title>Spruce and beech bark aqueous extracts: source of polyphenols, tannins and antioxidants correlated to in vitro antitumor potential on two different cell lines</title><title>Wood science and technology</title><addtitle>Wood Sci Technol</addtitle><description>The purpose of the present study was to determine the antioxidant capacity, the total phenol content, tannins, the cytotoxic and antiproliferative activity of some aqueous extracts obtained from spruce bark (
Picea abies
L.) and beech bark (
Fagus sylvatica
L.). In this regard, two types of extracts were obtained from both species using different methods of extraction: classical water bath extraction and ultrasound-assisted extraction. The antioxidant activity (AOA) was determined by means of DPPH assay and the cytotoxic and antiproliferative effects were determined using Alamar Blue technique and scratch assay. The total phenol content was determined colorimetrically by means of the Folin–Ciocalteu method. In vitro antitumor activity was tested on two different cell lines: A375 (human melanoma) and A549 (lung carcinoma) because of their different origin and metastatic potential. In addition, a non-tumor cell line (HaCaT human keratinocytes) was used in order to evaluate the samples selectivity. The results indicated that the aqueous extracts obtained by classical extraction had a higher antioxidant activity compared to the extracts obtained by ultrasound-assisted extraction. Moreover, the AOA of
Picea abies
L. bark extracts proved to be superior to those obtained from
Fagus sylvatica
L. bark extracts. Regarding the effect on cancer cells, the samples elicited a dose-dependent cytotoxic effect on A375 human melanoma cells, while on A549 lung carcinoma cells, the extractive solutions stimulated tumor cells viability, an effect that was reduced by increasing the concentration of the samples. Furthermore, the extractive solutions proved to possess antiproliferative properties when tested at the highest concentration; results that could be correlated with the antioxidant effects. In the case of the non-tumor cell line, the samples did not display a cytotoxic activity and stimulated cells proliferation.</description><subject>Anticancer properties</subject><subject>Antioxidants</subject><subject>Antitumor activity</subject><subject>Bark</subject><subject>Beech</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cancer</subject><subject>Cell proliferation</subject><subject>Ceramics</subject><subject>Colorimetry</subject><subject>Composites</subject><subject>Cytotoxicity</subject><subject>Fagus sylvatica</subject><subject>Glass</subject><subject>In vitro methods and tests</subject><subject>Keratinocytes</subject><subject>Life Sciences</subject><subject>Lung carcinoma</subject><subject>Lungs</subject><subject>Machines</subject><subject>Manufacturing</subject><subject>Melanoma</subject><subject>Metastases</subject><subject>Natural Materials</subject><subject>Original</subject><subject>Phenols</subject><subject>Picea abies</subject><subject>Plant extracts</subject><subject>Polyphenols</subject><subject>Processes</subject><subject>Selectivity</subject><subject>Tannins</subject><subject>Tumor cell lines</subject><subject>Tumor cells</subject><subject>Ultrasonic imaging</subject><subject>Ultrasound</subject><subject>Viability</subject><subject>Water baths</subject><subject>Wood Science & Technology</subject><issn>0043-7719</issn><issn>1432-5225</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UcFO3DAQtVCR2C58QG8j9dqAx3GSTW8VAloJqQfgbHntcTfbYKe2U-Bn-NZ6d5E49fQ0T--90cxj7BPyc-S8u0icC9FWHFcV8g6r5ogtUNaiaoRoPrAF57Kuug77E_YxpS3n2HVytWCvd1OcDYH2FtZEZgNrHX-D_jNTmBPQc47a5PQVUphj0QUHUxhfpg35MKYvkLX3g097v_Z5CM-DLZjAhBhp1Jks5ACDh79DjmGvyfNjiCUmUxn0CMFDfgpgB-coFg4MjSOMg6d0yo6dHhOdveGSPVxf3V9-r25_3vy4_HZbmbqpc2W1EFpLY9etFW1DpieUUu5I51C3zmopam1xN_Kma61tEXvJ1yid7Ot6yT4fcqcYyukpq22515eVStSrHjtsywOXDA8qE0NKkZya4vCo44tCrnY1qEMNqtSgdjWopnjEwZOK1v-i-J78f9M_x8qOxw</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Coșarcă, Sanda-Liliana</creator><creator>Moacă, Elena-Alina</creator><creator>Tanase, Corneliu</creator><creator>Muntean, Daniela Lucia</creator><creator>Pavel, Ioana Zinuca</creator><creator>Dehelean, Cristina Adriana</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>KB.</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope></search><sort><creationdate>20190301</creationdate><title>Spruce and beech bark aqueous extracts: source of polyphenols, tannins and antioxidants correlated to in vitro antitumor potential on two different cell lines</title><author>Coșarcă, Sanda-Liliana ; Moacă, Elena-Alina ; Tanase, Corneliu ; Muntean, Daniela Lucia ; Pavel, Ioana Zinuca ; Dehelean, Cristina Adriana</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-da22aa4cdb6d265ec9e144422aaff1a6fda423ad1aff10576dd611940b14f4933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anticancer properties</topic><topic>Antioxidants</topic><topic>Antitumor activity</topic><topic>Bark</topic><topic>Beech</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cancer</topic><topic>Cell proliferation</topic><topic>Ceramics</topic><topic>Colorimetry</topic><topic>Composites</topic><topic>Cytotoxicity</topic><topic>Fagus sylvatica</topic><topic>Glass</topic><topic>In vitro methods and tests</topic><topic>Keratinocytes</topic><topic>Life Sciences</topic><topic>Lung carcinoma</topic><topic>Lungs</topic><topic>Machines</topic><topic>Manufacturing</topic><topic>Melanoma</topic><topic>Metastases</topic><topic>Natural Materials</topic><topic>Original</topic><topic>Phenols</topic><topic>Picea abies</topic><topic>Plant extracts</topic><topic>Polyphenols</topic><topic>Processes</topic><topic>Selectivity</topic><topic>Tannins</topic><topic>Tumor cell lines</topic><topic>Tumor cells</topic><topic>Ultrasonic imaging</topic><topic>Ultrasound</topic><topic>Viability</topic><topic>Water baths</topic><topic>Wood Science & Technology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Coșarcă, Sanda-Liliana</creatorcontrib><creatorcontrib>Moacă, Elena-Alina</creatorcontrib><creatorcontrib>Tanase, Corneliu</creatorcontrib><creatorcontrib>Muntean, Daniela Lucia</creatorcontrib><creatorcontrib>Pavel, Ioana Zinuca</creatorcontrib><creatorcontrib>Dehelean, Cristina Adriana</creatorcontrib><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Materials Science Database</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><jtitle>Wood science and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Coșarcă, Sanda-Liliana</au><au>Moacă, Elena-Alina</au><au>Tanase, Corneliu</au><au>Muntean, Daniela Lucia</au><au>Pavel, Ioana Zinuca</au><au>Dehelean, Cristina Adriana</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spruce and beech bark aqueous extracts: source of polyphenols, tannins and antioxidants correlated to in vitro antitumor potential on two different cell lines</atitle><jtitle>Wood science and technology</jtitle><stitle>Wood Sci Technol</stitle><date>2019-03-01</date><risdate>2019</risdate><volume>53</volume><issue>2</issue><spage>313</spage><epage>333</epage><pages>313-333</pages><issn>0043-7719</issn><eissn>1432-5225</eissn><abstract>The purpose of the present study was to determine the antioxidant capacity, the total phenol content, tannins, the cytotoxic and antiproliferative activity of some aqueous extracts obtained from spruce bark (
Picea abies
L.) and beech bark (
Fagus sylvatica
L.). In this regard, two types of extracts were obtained from both species using different methods of extraction: classical water bath extraction and ultrasound-assisted extraction. The antioxidant activity (AOA) was determined by means of DPPH assay and the cytotoxic and antiproliferative effects were determined using Alamar Blue technique and scratch assay. The total phenol content was determined colorimetrically by means of the Folin–Ciocalteu method. In vitro antitumor activity was tested on two different cell lines: A375 (human melanoma) and A549 (lung carcinoma) because of their different origin and metastatic potential. In addition, a non-tumor cell line (HaCaT human keratinocytes) was used in order to evaluate the samples selectivity. The results indicated that the aqueous extracts obtained by classical extraction had a higher antioxidant activity compared to the extracts obtained by ultrasound-assisted extraction. Moreover, the AOA of
Picea abies
L. bark extracts proved to be superior to those obtained from
Fagus sylvatica
L. bark extracts. Regarding the effect on cancer cells, the samples elicited a dose-dependent cytotoxic effect on A375 human melanoma cells, while on A549 lung carcinoma cells, the extractive solutions stimulated tumor cells viability, an effect that was reduced by increasing the concentration of the samples. Furthermore, the extractive solutions proved to possess antiproliferative properties when tested at the highest concentration; results that could be correlated with the antioxidant effects. In the case of the non-tumor cell line, the samples did not display a cytotoxic activity and stimulated cells proliferation.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00226-018-1071-5</doi><tpages>21</tpages></addata></record> |
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| ispartof | Wood science and technology, 2019-03, Vol.53 (2), p.313-333 |
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| subjects | Anticancer properties Antioxidants Antitumor activity Bark Beech Biomedical and Life Sciences Biotechnology Cancer Cell proliferation Ceramics Colorimetry Composites Cytotoxicity Fagus sylvatica Glass In vitro methods and tests Keratinocytes Life Sciences Lung carcinoma Lungs Machines Manufacturing Melanoma Metastases Natural Materials Original Phenols Picea abies Plant extracts Polyphenols Processes Selectivity Tannins Tumor cell lines Tumor cells Ultrasonic imaging Ultrasound Viability Water baths Wood Science & Technology |
| title | Spruce and beech bark aqueous extracts: source of polyphenols, tannins and antioxidants correlated to in vitro antitumor potential on two different cell lines |
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