Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway
The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell li...
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| Veröffentlicht in: | Oncology reports 2020-05, Vol.43 (5), p.1683-1691 |
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| creator | Xu, Shenglin Wang, Yuan Que, Yukang Ma, Chengxiao Cai, Shang Wang, Hao Yang, Xingyu Yang, Chunjun Cheng, Cheng Zhao, Guoping Hu, Yong |
| description | The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis. |
| doi_str_mv | 10.3892/or.2020.7518 |
| format | Article |
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CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or.2020.7518</identifier><identifier>PMID: 32323810</identifier><language>eng</language><publisher>Greece: Spandidos Publications</publisher><subject>Apoptosis ; Bone cancer ; Brain Neoplasms - drug therapy ; Brain Neoplasms - metabolism ; Cancer therapies ; Cell culture ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Survival - drug effects ; Chemotherapy ; Cytochrome c ; Cytochromes c - metabolism ; EDTA ; Electrodes ; Flow cytometry ; Gene Expression Regulation, Neoplastic - drug effects ; Humans ; Medical prognosis ; Membrane Potential, Mitochondrial - drug effects ; Mitochondria - metabolism ; Monosaccharides ; Osteosarcoma ; Osteosarcoma - drug therapy ; Osteosarcoma - metabolism ; Plasma Gases - pharmacology ; Reactive oxygen species ; Reactive Oxygen Species - metabolism ; Ringer's Solution - pharmacology ; Sarcoma ; Time ; Tumors</subject><ispartof>Oncology reports, 2020-05, Vol.43 (5), p.1683-1691</ispartof><rights>COPYRIGHT 2020 Spandidos Publications</rights><rights>Copyright Spandidos Publications UK Ltd. 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-c8a1823dbd4b34ddf695f9c2168d37e3ed261bbec2bee9f8e698002b3a0197a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32323810$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Shenglin</creatorcontrib><creatorcontrib>Wang, Yuan</creatorcontrib><creatorcontrib>Que, Yukang</creatorcontrib><creatorcontrib>Ma, Chengxiao</creatorcontrib><creatorcontrib>Cai, Shang</creatorcontrib><creatorcontrib>Wang, Hao</creatorcontrib><creatorcontrib>Yang, Xingyu</creatorcontrib><creatorcontrib>Yang, Chunjun</creatorcontrib><creatorcontrib>Cheng, Cheng</creatorcontrib><creatorcontrib>Zhao, Guoping</creatorcontrib><creatorcontrib>Hu, Yong</creatorcontrib><title>Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>The present study aimed to investigate the effects of cold atmospheric plasma (CAP)‑activated Ringer's solution on osteosarcoma cell lines MG63 and U2OS, and to identify the molecular mechanism underlying these effects. CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis.</description><subject>Apoptosis</subject><subject>Bone cancer</subject><subject>Brain Neoplasms - drug therapy</subject><subject>Brain Neoplasms - metabolism</subject><subject>Cancer therapies</subject><subject>Cell culture</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival - drug effects</subject><subject>Chemotherapy</subject><subject>Cytochrome c</subject><subject>Cytochromes c - metabolism</subject><subject>EDTA</subject><subject>Electrodes</subject><subject>Flow cytometry</subject><subject>Gene Expression Regulation, Neoplastic - drug effects</subject><subject>Humans</subject><subject>Medical prognosis</subject><subject>Membrane Potential, Mitochondrial - drug effects</subject><subject>Mitochondria - metabolism</subject><subject>Monosaccharides</subject><subject>Osteosarcoma</subject><subject>Osteosarcoma - drug therapy</subject><subject>Osteosarcoma - metabolism</subject><subject>Plasma Gases - pharmacology</subject><subject>Reactive oxygen species</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Ringer's Solution - pharmacology</subject><subject>Sarcoma</subject><subject>Time</subject><subject>Tumors</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNptkctu1TAQhiMEoqWwY40sIcGGHHw5SZxldcRNqoSEumBnTezJiSsnE2wH1B2vAI_Ik5DTC1AJzcKW55sZz_8XxVPBN0q38jXFjeSSb5pK6HvFsWhaUcqtEvfXO5eiVKr6fFQ8SumCc9nwun1YHCm5hhb8uPi5o-AY5JHSPGD0ls0B0gi_vv8Am_1XyOjYJz_tMb5MLFFYsqeJ-Wnwnc-J5QHZHCn4HiNcpahnlDJSgmhpBGYxhAMXadkPV_zoM9mBJhc9BAYzzZmST2yGPHyDy8fFgx5Cwic350lx_vbN-e59efbx3Yfd6VlpKylyaTUILZXr3LZTW-f6uq361kpRa6caVOhkLboOrewQ215j3epVgU4BF20D6qR4ft12_f6XBVM2F7TEaZ1oVm0aWTWq4n-pPQQ0fuopR7CjT9ac1rLStdK1XqnNf6g1HI7e0oS9X9_vFLz4p2BACHm4FTfdBV9dgzZSShF7M0c_Qrw0gpuD_4aiOfhvDv6v-LObpZZuRPcHvjVc_QamUK6f</recordid><startdate>20200501</startdate><enddate>20200501</enddate><creator>Xu, Shenglin</creator><creator>Wang, Yuan</creator><creator>Que, Yukang</creator><creator>Ma, Chengxiao</creator><creator>Cai, Shang</creator><creator>Wang, Hao</creator><creator>Yang, Xingyu</creator><creator>Yang, Chunjun</creator><creator>Cheng, Cheng</creator><creator>Zhao, Guoping</creator><creator>Hu, Yong</creator><general>Spandidos Publications</general><general>Spandidos Publications UK Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20200501</creationdate><title>Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway</title><author>Xu, Shenglin ; 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CAP‑activated Ringer's solution was used to treat osteosarcoma cell lines MG63 and U2OS for 30 min. Cell viability was measured using the MTT method. The apoptosis rate was detected using Annexin‑V and propidium iodide. The expression levels of cytochrome c, caspase‑3 and polyADP ribose polymerase (PARP) in MG63 cells were analyzed via western blotting. The change in mitochondrial membrane potential was detected via the JC‑1 dye method and verified by the level of reactive oxygen species (ROS). CAP‑activated Ringer's solution inhibited the proliferation of MG63 and U2OS cells in a dose‑ and time‑dependent manner. Furthermore, CAP‑activated Ringer's solution induced the apoptosis of MG63 cells, increased the intracellular ROS level, decreased the mitochondrial membrane potential level, and induced the release of cytochrome c. CAP‑activated Ringer's solution inhibits osteosarcoma cell proliferation through intracellular ROS‑mediated mitochondrial apoptosis.</abstract><cop>Greece</cop><pub>Spandidos Publications</pub><pmid>32323810</pmid><doi>10.3892/or.2020.7518</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Apoptosis Bone cancer Brain Neoplasms - drug therapy Brain Neoplasms - metabolism Cancer therapies Cell culture Cell Line, Tumor Cell Proliferation - drug effects Cell Survival - drug effects Chemotherapy Cytochrome c Cytochromes c - metabolism EDTA Electrodes Flow cytometry Gene Expression Regulation, Neoplastic - drug effects Humans Medical prognosis Membrane Potential, Mitochondrial - drug effects Mitochondria - metabolism Monosaccharides Osteosarcoma Osteosarcoma - drug therapy Osteosarcoma - metabolism Plasma Gases - pharmacology Reactive oxygen species Reactive Oxygen Species - metabolism Ringer's Solution - pharmacology Sarcoma Time Tumors |
| title | Cold atmospheric plasma‑activated Ringer's solution inhibits the proliferation of osteosarcoma cells through the mitochondrial apoptosis pathway |
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