Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing
Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone...
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| Veröffentlicht in: | Journal of cellular physiology 2020-07, Vol.235 (7-8), p.5555-5569 |
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| creator | Joseph, Anand Baiju, Indu Bhat, Irfan A. Pandey, Sriti Bharti, Mukesh Verma, Megha Pratap Singh, Anuj Ansari, Matin M. Chandra, Vikash Saikumar, Gutulla Amarpal Taru Sharma, Gutulla |
| description | Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing.
Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains important biomolecules.
Lyophilized MSCs‐CM mixed with gel can be applied allogenically as well as xenogenically for quality wound healing.
It has better application than MSCs like; can be used fresh, lyophilized and stored at 4°C or −20°C, can be used in the form of gel‐like formulations, and at field level, it can be used by semiskilled personnel also. |
| doi_str_mv | 10.1002/jcp.29486 |
| format | Article |
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Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains important biomolecules.
Lyophilized MSCs‐CM mixed with gel can be applied allogenically as well as xenogenically for quality wound healing.
It has better application than MSCs like; can be used fresh, lyophilized and stored at 4°C or −20°C, can be used in the form of gel‐like formulations, and at field level, it can be used by semiskilled personnel also.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.29486</identifier><identifier>PMID: 31960454</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Animals ; Assaying ; Biomolecules ; Bone healing ; Bone marrow ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Cell Differentiation - genetics ; Cell Proliferation - genetics ; Cell therapy ; Cell- and Tissue-Based Therapy ; conditioned media ; Culture Media, Conditioned - pharmacology ; Cytokines ; Dogs ; Drying ; Epidermal growth factor ; Fibroblast growth factors ; Fibroblasts - drug effects ; Gene Expression Regulation, Developmental - drug effects ; Goats - genetics ; Growth factors ; Guinea Pigs ; Humans ; Immunohistochemistry ; Insulin ; Interleukin-6 - genetics ; Interleukins ; Laminin ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchymal Stem Cells - drug effects ; Stem cell factor ; Stem Cell Transplantation ; Stem cells ; Swine ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - genetics ; Wound healing ; Wound Healing - genetics ; Xenografts</subject><ispartof>Journal of cellular physiology, 2020-07, Vol.235 (7-8), p.5555-5569</ispartof><rights>2020 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4196-a4ec27f444d8a6ec1b970c552557d21df6dd075010dd1a1a2c3e5f02d03f61693</citedby><cites>FETCH-LOGICAL-c4196-a4ec27f444d8a6ec1b970c552557d21df6dd075010dd1a1a2c3e5f02d03f61693</cites><orcidid>0000-0001-6492-4673 ; 0000-0002-2142-3024 ; 0000-0002-5052-9477</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.29486$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.29486$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31960454$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Joseph, Anand</creatorcontrib><creatorcontrib>Baiju, Indu</creatorcontrib><creatorcontrib>Bhat, Irfan A.</creatorcontrib><creatorcontrib>Pandey, Sriti</creatorcontrib><creatorcontrib>Bharti, Mukesh</creatorcontrib><creatorcontrib>Verma, Megha</creatorcontrib><creatorcontrib>Pratap Singh, Anuj</creatorcontrib><creatorcontrib>Ansari, Matin M.</creatorcontrib><creatorcontrib>Chandra, Vikash</creatorcontrib><creatorcontrib>Saikumar, Gutulla</creatorcontrib><creatorcontrib>Amarpal</creatorcontrib><creatorcontrib>Taru Sharma, Gutulla</creatorcontrib><title>Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing.
Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains important biomolecules.
Lyophilized MSCs‐CM mixed with gel can be applied allogenically as well as xenogenically for quality wound healing.
It has better application than MSCs like; can be used fresh, lyophilized and stored at 4°C or −20°C, can be used in the form of gel‐like formulations, and at field level, it can be used by semiskilled personnel also.</description><subject>Animals</subject><subject>Assaying</subject><subject>Biomolecules</subject><subject>Bone healing</subject><subject>Bone marrow</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - drug effects</subject><subject>Cell Differentiation - genetics</subject><subject>Cell Proliferation - genetics</subject><subject>Cell therapy</subject><subject>Cell- and Tissue-Based Therapy</subject><subject>conditioned media</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Cytokines</subject><subject>Dogs</subject><subject>Drying</subject><subject>Epidermal growth factor</subject><subject>Fibroblast growth factors</subject><subject>Fibroblasts - drug effects</subject><subject>Gene Expression Regulation, Developmental - drug effects</subject><subject>Goats - genetics</subject><subject>Growth factors</subject><subject>Guinea Pigs</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Insulin</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukins</subject><subject>Laminin</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchymal Stem Cells - drug effects</subject><subject>Stem cell factor</subject><subject>Stem Cell Transplantation</subject><subject>Stem cells</subject><subject>Swine</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><subject>Wound healing</subject><subject>Wound Healing - genetics</subject><subject>Xenografts</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1OwzAQRi0EoqWw4ALIEisWKbZjOw27quJXRbCAdeTaE5rKids4aZUdR-CMnISUFFixGo3mzadPD6FTSoaUEHa50Mshi_lI7qE-JXEUcCnYPuq3NxrEgtMeOvJ-QQiJ4zA8RL2QxpJwwfsofwQPhZ43ubLYV5BjDdZ-vn9oV5isylwBBudgMnWFx7hwa7BY2QrKQlXZGrBL_75wNYdSLRucuhKvamWzqsEbVxcGz6HdirdjdJAq6-FkNwfo9eb6ZXIXTJ9u7yfjaaB52yxQHDSLUs65GSkJms7iiGghmBCRYdSk0hgSCUKJMVRRxXQIIiXMkDCVVMbhAJ13ucvSrWrwVbJwdVvZ-oSFI0lJRNmWuugoXTrvS0iTZZnlqmwSSpKt2KQVm3yLbdmzXWI9a3X8kj8mW-CyAzaZheb_pORh8txFfgEPZYPD</recordid><startdate>202007</startdate><enddate>202007</enddate><creator>Joseph, Anand</creator><creator>Baiju, Indu</creator><creator>Bhat, Irfan A.</creator><creator>Pandey, Sriti</creator><creator>Bharti, Mukesh</creator><creator>Verma, Megha</creator><creator>Pratap Singh, Anuj</creator><creator>Ansari, Matin M.</creator><creator>Chandra, Vikash</creator><creator>Saikumar, Gutulla</creator><creator>Amarpal</creator><creator>Taru Sharma, Gutulla</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0001-6492-4673</orcidid><orcidid>https://orcid.org/0000-0002-2142-3024</orcidid><orcidid>https://orcid.org/0000-0002-5052-9477</orcidid></search><sort><creationdate>202007</creationdate><title>Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing</title><author>Joseph, Anand ; Baiju, Indu ; Bhat, Irfan A. ; Pandey, Sriti ; Bharti, Mukesh ; Verma, Megha ; Pratap Singh, Anuj ; Ansari, Matin M. ; Chandra, Vikash ; Saikumar, Gutulla ; Amarpal ; Taru Sharma, Gutulla</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4196-a4ec27f444d8a6ec1b970c552557d21df6dd075010dd1a1a2c3e5f02d03f61693</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Animals</topic><topic>Assaying</topic><topic>Biomolecules</topic><topic>Bone healing</topic><topic>Bone marrow</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - drug effects</topic><topic>Cell Differentiation - genetics</topic><topic>Cell Proliferation - genetics</topic><topic>Cell therapy</topic><topic>Cell- and Tissue-Based Therapy</topic><topic>conditioned media</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Cytokines</topic><topic>Dogs</topic><topic>Drying</topic><topic>Epidermal growth factor</topic><topic>Fibroblast growth factors</topic><topic>Fibroblasts - drug effects</topic><topic>Gene Expression Regulation, Developmental - drug effects</topic><topic>Goats - genetics</topic><topic>Growth factors</topic><topic>Guinea Pigs</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Insulin</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukins</topic><topic>Laminin</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchymal Stem Cells - drug effects</topic><topic>Stem cell factor</topic><topic>Stem Cell Transplantation</topic><topic>Stem cells</topic><topic>Swine</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><topic>Wound healing</topic><topic>Wound Healing - genetics</topic><topic>Xenografts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Joseph, Anand</creatorcontrib><creatorcontrib>Baiju, Indu</creatorcontrib><creatorcontrib>Bhat, Irfan A.</creatorcontrib><creatorcontrib>Pandey, Sriti</creatorcontrib><creatorcontrib>Bharti, Mukesh</creatorcontrib><creatorcontrib>Verma, Megha</creatorcontrib><creatorcontrib>Pratap Singh, Anuj</creatorcontrib><creatorcontrib>Ansari, Matin M.</creatorcontrib><creatorcontrib>Chandra, Vikash</creatorcontrib><creatorcontrib>Saikumar, Gutulla</creatorcontrib><creatorcontrib>Amarpal</creatorcontrib><creatorcontrib>Taru Sharma, Gutulla</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Joseph, Anand</au><au>Baiju, Indu</au><au>Bhat, Irfan A.</au><au>Pandey, Sriti</au><au>Bharti, Mukesh</au><au>Verma, Megha</au><au>Pratap Singh, Anuj</au><au>Ansari, Matin M.</au><au>Chandra, Vikash</au><au>Saikumar, Gutulla</au><au>Amarpal</au><au>Taru Sharma, Gutulla</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2020-07</date><risdate>2020</risdate><volume>235</volume><issue>7-8</issue><spage>5555</spage><epage>5569</epage><pages>5555-5569</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains several growth factors and cytokines, thus may be used as a better alternative to stem cell therapy, which needs to be elucidated. The present study was conducted to evaluate the therapeutic potential of caprine, canine, and guinea pig bone marrow‐derived MSCs‐CM in excision wound healing in a guinea pig model. MSCs were obtained from bone marrow, expanded ex vivo and characterized as per ISCT criteria. CM was collected assayed by western blot to ascertain the presence of important secretory biomolecules. Quantitative estimation by enzyme‐linked immunosorbent assay was done for a vascular epidermal growth factor (VEGF) and interleukin‐6 (IL‐6) in caprine MSCs‐CM and optimum time for collection of CM was decided as 72 hr. CM from all the species was lyophilized by freeze‐drying method. Full‐thickness (2 × 2 cm2) excision skin wounds were created in guinea pigs (six animals in each group) and respective lyophilized CM mixed with laminin gel was applied topically at weekly interval. On Day 28, histopathological examinations of healed skin were done by hemotoxylin and eosin staining. MSCs were found to secrete important growth factors and cytokines (i.e., VEGF, transforming growth factor‐β1, fibroblast growth factor‐2, insulin‐like growth factor‐1, stem cell factor, and IL‐6) as demonstrated by immunohistochemistry and western blot assay. It was found that allogenic and xenogenic application of CM significantly improved quality wound healing with minimal scar formation. Thus, MSCs‐CM can be used allogenically as well as xenogenically for quality wound healing.
Mesenchymal stem cells‐conditioned media (MSCs‐CM) contains important biomolecules.
Lyophilized MSCs‐CM mixed with gel can be applied allogenically as well as xenogenically for quality wound healing.
It has better application than MSCs like; can be used fresh, lyophilized and stored at 4°C or −20°C, can be used in the form of gel‐like formulations, and at field level, it can be used by semiskilled personnel also.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31960454</pmid><doi>10.1002/jcp.29486</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0001-6492-4673</orcidid><orcidid>https://orcid.org/0000-0002-2142-3024</orcidid><orcidid>https://orcid.org/0000-0002-5052-9477</orcidid></addata></record> |
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| subjects | Animals Assaying Biomolecules Bone healing Bone marrow Bone Marrow Cells - cytology Bone Marrow Cells - drug effects Cell Differentiation - genetics Cell Proliferation - genetics Cell therapy Cell- and Tissue-Based Therapy conditioned media Culture Media, Conditioned - pharmacology Cytokines Dogs Drying Epidermal growth factor Fibroblast growth factors Fibroblasts - drug effects Gene Expression Regulation, Developmental - drug effects Goats - genetics Growth factors Guinea Pigs Humans Immunohistochemistry Insulin Interleukin-6 - genetics Interleukins Laminin Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchymal Stem Cells - drug effects Stem cell factor Stem Cell Transplantation Stem cells Swine Vascular endothelial growth factor Vascular Endothelial Growth Factor A - genetics Wound healing Wound Healing - genetics Xenografts |
| title | Mesenchymal stem cell‐conditioned media: A novel alternative of stem cell therapy for quality wound healing |
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