Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge
Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression,...
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| Veröffentlicht in: | Journal of fish biology 2020-03, Vol.96 (3), p.731-746 |
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| creator | Lulijwa, Ronald Alfaro, Andrea C. Merien, Fabrice Burdass, Mark Meyer, Jill Venter, Leonie Young, Tim |
| description | Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies. |
| doi_str_mv | 10.1111/jfb.14266 |
| format | Article |
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Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.</description><identifier>ISSN: 0022-1112</identifier><identifier>EISSN: 1095-8649</identifier><identifier>DOI: 10.1111/jfb.14266</identifier><identifier>PMID: 31995234</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adaptive immunity ; Amino acids ; Antiviral agents ; Biomarkers ; Blood cells ; Chain branching ; Chinook salmon ; cytokine transcript expression ; Cytokines ; Energy metabolism ; Erythrocytes ; Fish ; fish immunology ; Freshwater fishes ; Gas chromatography ; Glutathione ; Glycolysis ; Hematology ; Immune response ; Immunity ; In vivo methods and tests ; Kidneys ; Leukocytes ; Levels ; Lipid metabolism ; Mass spectrometry ; Mass spectroscopy ; Metabolism ; Metabolites ; Metabolomics ; Myxovirus resistance proteins ; Nitric oxide ; Oncorhynchus tshawytscha ; Parameters ; peripheral blood mononuclear cells ; Phagocytes ; Phospholipids ; poly (I:C) ; Polyinosinic:polycytidylic acid ; Reactive oxygen species ; Salmon ; Serum ; Smolts ; Spleen ; Transcription ; γ-Interferon</subject><ispartof>Journal of fish biology, 2020-03, Vol.96 (3), p.731-746</ispartof><rights>2020 The Fisheries Society of the British Isles</rights><rights>2020 The Fisheries Society of the British Isles.</rights><rights>Journal of Fish Biology © 2020 The Fisheries Society of the British Isles</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4196-19bda91317831cbd88f906a3d8f329811ebeabf6ba79f72a6723776e6239e0e3</citedby><cites>FETCH-LOGICAL-c4196-19bda91317831cbd88f906a3d8f329811ebeabf6ba79f72a6723776e6239e0e3</cites><orcidid>0000-0003-0543-7212</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjfb.14266$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjfb.14266$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1413,27906,27907,45556,45557</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31995234$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lulijwa, Ronald</creatorcontrib><creatorcontrib>Alfaro, Andrea C.</creatorcontrib><creatorcontrib>Merien, Fabrice</creatorcontrib><creatorcontrib>Burdass, Mark</creatorcontrib><creatorcontrib>Meyer, Jill</creatorcontrib><creatorcontrib>Venter, Leonie</creatorcontrib><creatorcontrib>Young, Tim</creatorcontrib><title>Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge</title><title>Journal of fish biology</title><addtitle>J Fish Biol</addtitle><description>Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.</description><subject>Adaptive immunity</subject><subject>Amino acids</subject><subject>Antiviral agents</subject><subject>Biomarkers</subject><subject>Blood cells</subject><subject>Chain branching</subject><subject>Chinook salmon</subject><subject>cytokine transcript expression</subject><subject>Cytokines</subject><subject>Energy metabolism</subject><subject>Erythrocytes</subject><subject>Fish</subject><subject>fish immunology</subject><subject>Freshwater fishes</subject><subject>Gas chromatography</subject><subject>Glutathione</subject><subject>Glycolysis</subject><subject>Hematology</subject><subject>Immune response</subject><subject>Immunity</subject><subject>In vivo methods and tests</subject><subject>Kidneys</subject><subject>Leukocytes</subject><subject>Levels</subject><subject>Lipid metabolism</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Metabolomics</subject><subject>Myxovirus resistance proteins</subject><subject>Nitric oxide</subject><subject>Oncorhynchus tshawytscha</subject><subject>Parameters</subject><subject>peripheral blood mononuclear cells</subject><subject>Phagocytes</subject><subject>Phospholipids</subject><subject>poly (I:C)</subject><subject>Polyinosinic:polycytidylic acid</subject><subject>Reactive oxygen species</subject><subject>Salmon</subject><subject>Serum</subject><subject>Smolts</subject><subject>Spleen</subject><subject>Transcription</subject><subject>γ-Interferon</subject><issn>0022-1112</issn><issn>1095-8649</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp10DtOxDAQBmALgWBZKLgAskTDFgE_ghPTQcRTi7bZPnKSCcmS2IudaJWOI3BGToIhQMc0I40-_SP9CB1Rckb9nK_K7IyGTIgtNKFEXgSxCOU2mhDCWOAB20P7zq0IIZJLvov2OJXygvFwgoYn6FRmmjrHShe4btteA7bg1kY7cNiUOKlqbcwLdqppjcanC50bWw06r3qHO1epzdC5vFIz7FrTdP5msMKuMrb7eHvvwLZ4bZoBnz5cJjPsYdOAfoYDtFOqxsHhz56i5e3NMrkP5ou7h-RqHuQhlSKgMiuUpJxGMad5VsRxKYlQvIhLzmRMKWSgslJkKpJlxJSIGI8iAYJxCQT4FJ2MsWtrXntwXboyvdX-Y-phGDPul1ezUeXWOGehTNe2bpUdUkrSr45T33H63bG3xz-JfdZC8Sd_S_XgfASbuoHh_6T08fZ6jPwEtxKG_A</recordid><startdate>202003</startdate><enddate>202003</enddate><creator>Lulijwa, Ronald</creator><creator>Alfaro, Andrea C.</creator><creator>Merien, Fabrice</creator><creator>Burdass, Mark</creator><creator>Meyer, Jill</creator><creator>Venter, Leonie</creator><creator>Young, Tim</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>7TN</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><orcidid>https://orcid.org/0000-0003-0543-7212</orcidid></search><sort><creationdate>202003</creationdate><title>Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge</title><author>Lulijwa, Ronald ; Alfaro, Andrea C. ; Merien, Fabrice ; Burdass, Mark ; Meyer, Jill ; Venter, Leonie ; Young, Tim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4196-19bda91317831cbd88f906a3d8f329811ebeabf6ba79f72a6723776e6239e0e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adaptive immunity</topic><topic>Amino acids</topic><topic>Antiviral agents</topic><topic>Biomarkers</topic><topic>Blood cells</topic><topic>Chain branching</topic><topic>Chinook salmon</topic><topic>cytokine transcript expression</topic><topic>Cytokines</topic><topic>Energy metabolism</topic><topic>Erythrocytes</topic><topic>Fish</topic><topic>fish immunology</topic><topic>Freshwater fishes</topic><topic>Gas chromatography</topic><topic>Glutathione</topic><topic>Glycolysis</topic><topic>Hematology</topic><topic>Immune response</topic><topic>Immunity</topic><topic>In vivo methods and tests</topic><topic>Kidneys</topic><topic>Leukocytes</topic><topic>Levels</topic><topic>Lipid metabolism</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Metabolomics</topic><topic>Myxovirus resistance proteins</topic><topic>Nitric oxide</topic><topic>Oncorhynchus tshawytscha</topic><topic>Parameters</topic><topic>peripheral blood mononuclear cells</topic><topic>Phagocytes</topic><topic>Phospholipids</topic><topic>poly (I:C)</topic><topic>Polyinosinic:polycytidylic acid</topic><topic>Reactive oxygen species</topic><topic>Salmon</topic><topic>Serum</topic><topic>Smolts</topic><topic>Spleen</topic><topic>Transcription</topic><topic>γ-Interferon</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lulijwa, Ronald</creatorcontrib><creatorcontrib>Alfaro, Andrea C.</creatorcontrib><creatorcontrib>Merien, Fabrice</creatorcontrib><creatorcontrib>Burdass, Mark</creatorcontrib><creatorcontrib>Meyer, Jill</creatorcontrib><creatorcontrib>Venter, Leonie</creatorcontrib><creatorcontrib>Young, Tim</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of fish biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lulijwa, Ronald</au><au>Alfaro, Andrea C.</au><au>Merien, Fabrice</au><au>Burdass, Mark</au><au>Meyer, Jill</au><au>Venter, Leonie</au><au>Young, Tim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge</atitle><jtitle>Journal of fish biology</jtitle><addtitle>J Fish Biol</addtitle><date>2020-03</date><risdate>2020</risdate><volume>96</volume><issue>3</issue><spage>731</spage><epage>746</epage><pages>731-746</pages><issn>0022-1112</issn><eissn>1095-8649</eissn><abstract>Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook salmon (Oncorhynchus tshawytscha) post‐smolts to determine the immune responses on haematological and cellular functional parameters, including spleen (SP), head kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish haematological parameters, leucocyte phagocytic activity and phagocytic index, reactive oxygen species and nitric oxide production. Gas chromatography–mass spectrometry–based metabolomics revealed that poly (I:C) significantly altered the serum biochemistry profile of 25 metabolites. Metabolites involved in the branched‐chain amino acid/glutathione and transsulphuration pathways and phospholipid metabolism accumulated in poly (I:C)–treated fish, whereas those involved in the glycolytic and energy metabolism pathways were downregulated. At cytokine transcript level, poly (I:C) induced a significant upregulation of antiviral ifnγ in HK and Mx1 protein in HK, SP and RBCs. This study provides evidence for poly (I:C)–induced, immune‐related biomarkers at metabolic and molecular levels in farmed O. tshawytscha in vivo. These findings provide insights into short‐term effects of poly (I:C) at haematological, innate and adaptive immunity and metabolic levels, setting the stage for future studies.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>31995234</pmid><doi>10.1111/jfb.14266</doi><tpages>16</tpages><orcidid>https://orcid.org/0000-0003-0543-7212</orcidid></addata></record> |
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| subjects | Adaptive immunity Amino acids Antiviral agents Biomarkers Blood cells Chain branching Chinook salmon cytokine transcript expression Cytokines Energy metabolism Erythrocytes Fish fish immunology Freshwater fishes Gas chromatography Glutathione Glycolysis Hematology Immune response Immunity In vivo methods and tests Kidneys Leukocytes Levels Lipid metabolism Mass spectrometry Mass spectroscopy Metabolism Metabolites Metabolomics Myxovirus resistance proteins Nitric oxide Oncorhynchus tshawytscha Parameters peripheral blood mononuclear cells Phagocytes Phospholipids poly (I:C) Polyinosinic:polycytidylic acid Reactive oxygen species Salmon Serum Smolts Spleen Transcription γ-Interferon |
| title | Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) smolts to a short‐term poly (I:C) challenge |
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