Hair keratin promotes wound healing in rats with combined radiation-wound injury

Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound heali...

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Veröffentlicht in:Journal of materials science. Materials in medicine 2020-03, Vol.31 (3), p.28, Article 28
Hauptverfasser: Chen, Xiaoliang, Zhai, Dongliang, Wang, Bochu, Hao, Shilei, Song, Jia, Peng, Zhiping
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container_title Journal of materials science. Materials in medicine
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creator Chen, Xiaoliang
Zhai, Dongliang
Wang, Bochu
Hao, Shilei
Song, Jia
Peng, Zhiping
description Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.
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However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-020-06365-x</identifier><identifier>PMID: 32125534</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acrylic Resins - chemistry ; Amino acids ; Animals ; Biocompatible Materials - chemistry ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Cell Proliferation - drug effects ; Cell Survival ; Cell viability ; Ceramics ; Chemistry and Materials Science ; Composites ; Cytokines - metabolism ; Electrophoresis ; Fourier analysis ; Fourier transforms ; Gel electrophoresis ; Glass ; HaCaT Cells ; Hair ; Hair - chemistry ; Humans ; Hydrogels ; Hydrogels - chemistry ; In vivo methods and tests ; Inflammation ; Infrared analysis ; Infrared spectroscopy ; Keratin ; Keratins, Hair-Specific - chemistry ; Keratins, Hair-Specific - therapeutic use ; Leukocyte Count ; Materials Science ; Microscopy, Electron, Scanning ; Natural Materials ; Polyacrylamide ; Polymer Sciences ; Radiation ; Radiation effects ; Radiation injuries ; Radiation Injuries - therapy ; Rats ; Regeneration ; Regenerative Medicine/Tissue Engineering ; Repair ; Rheological properties ; Rheology ; Sodium Dodecyl Sulfate - chemistry ; Spectroscopy, Fourier Transform Infrared ; Sulfates ; Surfaces and Interfaces ; Thin Films ; Time Factors ; Tissue engineering ; Tissue Engineering Constructs and Cell Substrates ; Wound healing ; Wound Healing - drug effects</subject><ispartof>Journal of materials science. 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Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. 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Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32125534</pmid><doi>10.1007/s10856-020-06365-x</doi></addata></record>
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subjects Acrylic Resins - chemistry
Amino acids
Animals
Biocompatible Materials - chemistry
Biomaterials
Biomedical Engineering and Bioengineering
Biomedical materials
Cell Proliferation - drug effects
Cell Survival
Cell viability
Ceramics
Chemistry and Materials Science
Composites
Cytokines - metabolism
Electrophoresis
Fourier analysis
Fourier transforms
Gel electrophoresis
Glass
HaCaT Cells
Hair
Hair - chemistry
Humans
Hydrogels
Hydrogels - chemistry
In vivo methods and tests
Inflammation
Infrared analysis
Infrared spectroscopy
Keratin
Keratins, Hair-Specific - chemistry
Keratins, Hair-Specific - therapeutic use
Leukocyte Count
Materials Science
Microscopy, Electron, Scanning
Natural Materials
Polyacrylamide
Polymer Sciences
Radiation
Radiation effects
Radiation injuries
Radiation Injuries - therapy
Rats
Regeneration
Regenerative Medicine/Tissue Engineering
Repair
Rheological properties
Rheology
Sodium Dodecyl Sulfate - chemistry
Spectroscopy, Fourier Transform Infrared
Sulfates
Surfaces and Interfaces
Thin Films
Time Factors
Tissue engineering
Tissue Engineering Constructs and Cell Substrates
Wound healing
Wound Healing - drug effects
title Hair keratin promotes wound healing in rats with combined radiation-wound injury
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