Hair keratin promotes wound healing in rats with combined radiation-wound injury
Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound heali...
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creator | Chen, Xiaoliang Zhai, Dongliang Wang, Bochu Hao, Shilei Song, Jia Peng, Zhiping |
description | Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins. |
doi_str_mv | 10.1007/s10856-020-06365-x |
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However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-020-06365-x</identifier><identifier>PMID: 32125534</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Acrylic Resins - chemistry ; Amino acids ; Animals ; Biocompatible Materials - chemistry ; Biomaterials ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Cell Proliferation - drug effects ; Cell Survival ; Cell viability ; Ceramics ; Chemistry and Materials Science ; Composites ; Cytokines - metabolism ; Electrophoresis ; Fourier analysis ; Fourier transforms ; Gel electrophoresis ; Glass ; HaCaT Cells ; Hair ; Hair - chemistry ; Humans ; Hydrogels ; Hydrogels - chemistry ; In vivo methods and tests ; Inflammation ; Infrared analysis ; Infrared spectroscopy ; Keratin ; Keratins, Hair-Specific - chemistry ; Keratins, Hair-Specific - therapeutic use ; Leukocyte Count ; Materials Science ; Microscopy, Electron, Scanning ; Natural Materials ; Polyacrylamide ; Polymer Sciences ; Radiation ; Radiation effects ; Radiation injuries ; Radiation Injuries - therapy ; Rats ; Regeneration ; Regenerative Medicine/Tissue Engineering ; Repair ; Rheological properties ; Rheology ; Sodium Dodecyl Sulfate - chemistry ; Spectroscopy, Fourier Transform Infrared ; Sulfates ; Surfaces and Interfaces ; Thin Films ; Time Factors ; Tissue engineering ; Tissue Engineering Constructs and Cell Substrates ; Wound healing ; Wound Healing - drug effects</subject><ispartof>Journal of materials science. Materials in medicine, 2020-03, Vol.31 (3), p.28, Article 28</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2020</rights><rights>Journal of Materials Science: Materials in Medicine is a copyright of Springer, (2020). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-83d1f828178ce56445a84b522da551ac6088d09a76e837d9b94f5514964aa87e3</citedby><cites>FETCH-LOGICAL-c414t-83d1f828178ce56445a84b522da551ac6088d09a76e837d9b94f5514964aa87e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10856-020-06365-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10856-020-06365-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27923,27924,41487,42556,51318</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32125534$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chen, Xiaoliang</creatorcontrib><creatorcontrib>Zhai, Dongliang</creatorcontrib><creatorcontrib>Wang, Bochu</creatorcontrib><creatorcontrib>Hao, Shilei</creatorcontrib><creatorcontrib>Song, Jia</creatorcontrib><creatorcontrib>Peng, Zhiping</creatorcontrib><title>Hair keratin promotes wound healing in rats with combined radiation-wound injury</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.</description><subject>Acrylic Resins - chemistry</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biomaterials</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival</subject><subject>Cell viability</subject><subject>Ceramics</subject><subject>Chemistry and Materials Science</subject><subject>Composites</subject><subject>Cytokines - metabolism</subject><subject>Electrophoresis</subject><subject>Fourier analysis</subject><subject>Fourier transforms</subject><subject>Gel electrophoresis</subject><subject>Glass</subject><subject>HaCaT Cells</subject><subject>Hair</subject><subject>Hair - chemistry</subject><subject>Humans</subject><subject>Hydrogels</subject><subject>Hydrogels - chemistry</subject><subject>In vivo methods and tests</subject><subject>Inflammation</subject><subject>Infrared analysis</subject><subject>Infrared spectroscopy</subject><subject>Keratin</subject><subject>Keratins, Hair-Specific - chemistry</subject><subject>Keratins, Hair-Specific - therapeutic use</subject><subject>Leukocyte Count</subject><subject>Materials Science</subject><subject>Microscopy, Electron, Scanning</subject><subject>Natural Materials</subject><subject>Polyacrylamide</subject><subject>Polymer Sciences</subject><subject>Radiation</subject><subject>Radiation effects</subject><subject>Radiation injuries</subject><subject>Radiation Injuries - therapy</subject><subject>Rats</subject><subject>Regeneration</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Repair</subject><subject>Rheological properties</subject><subject>Rheology</subject><subject>Sodium Dodecyl Sulfate - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Sulfates</subject><subject>Surfaces and Interfaces</subject><subject>Thin Films</subject><subject>Time Factors</subject><subject>Tissue engineering</subject><subject>Tissue Engineering Constructs and Cell Substrates</subject><subject>Wound healing</subject><subject>Wound Healing - drug 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keratin promotes wound healing in rats with combined radiation-wound injury</title><author>Chen, Xiaoliang ; Zhai, Dongliang ; Wang, Bochu ; Hao, Shilei ; Song, Jia ; Peng, Zhiping</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-83d1f828178ce56445a84b522da551ac6088d09a76e837d9b94f5514964aa87e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acrylic Resins - chemistry</topic><topic>Amino acids</topic><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biomaterials</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Biomedical materials</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival</topic><topic>Cell viability</topic><topic>Ceramics</topic><topic>Chemistry and Materials Science</topic><topic>Composites</topic><topic>Cytokines - metabolism</topic><topic>Electrophoresis</topic><topic>Fourier analysis</topic><topic>Fourier transforms</topic><topic>Gel electrophoresis</topic><topic>Glass</topic><topic>HaCaT Cells</topic><topic>Hair</topic><topic>Hair - chemistry</topic><topic>Humans</topic><topic>Hydrogels</topic><topic>Hydrogels - chemistry</topic><topic>In vivo methods and tests</topic><topic>Inflammation</topic><topic>Infrared analysis</topic><topic>Infrared spectroscopy</topic><topic>Keratin</topic><topic>Keratins, Hair-Specific - chemistry</topic><topic>Keratins, Hair-Specific - therapeutic use</topic><topic>Leukocyte Count</topic><topic>Materials Science</topic><topic>Microscopy, Electron, Scanning</topic><topic>Natural Materials</topic><topic>Polyacrylamide</topic><topic>Polymer Sciences</topic><topic>Radiation</topic><topic>Radiation effects</topic><topic>Radiation injuries</topic><topic>Radiation Injuries - therapy</topic><topic>Rats</topic><topic>Regeneration</topic><topic>Regenerative Medicine/Tissue Engineering</topic><topic>Repair</topic><topic>Rheological 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Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Xiaoliang</au><au>Zhai, Dongliang</au><au>Wang, Bochu</au><au>Hao, Shilei</au><au>Song, Jia</au><au>Peng, Zhiping</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hair keratin promotes wound healing in rats with combined radiation-wound injury</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2020-03-03</date><risdate>2020</risdate><volume>31</volume><issue>3</issue><spage>28</spage><pages>28-</pages><artnum>28</artnum><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>Keratins derived from human hair have been suggested to be particularly effective in general surgical wound healing. However, the healing of a combined radiation-wound injury is a multifaceted regenerative process. Here, hydrogels fabricated with human hair keratins were used to test the wound healing effects on rats suffering from combined radiation-wound injuries. Briefly, the keratin extracts were verified by dodecyl sulfate polyacrylamide gel electrophoresis analysis and amino acid analysis, and the keratin hydrogels were then characterized by morphological observation, Fourier transform infrared spectroscopy analysis and rheology analyses. The results of the cell viability assay indicated that the keratin hydrogels could enhance cell growth after radiation exposure. Furthermore, keratin hydrogels could accelerate wound repair and improve the survival rate in vivo. The results demonstrate that keratin hydrogels possess a strong ability to accelerate the repair of a combined radiation-wound injury, which opens up new tissue regeneration applications for keratins.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>32125534</pmid><doi>10.1007/s10856-020-06365-x</doi></addata></record> |
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subjects | Acrylic Resins - chemistry Amino acids Animals Biocompatible Materials - chemistry Biomaterials Biomedical Engineering and Bioengineering Biomedical materials Cell Proliferation - drug effects Cell Survival Cell viability Ceramics Chemistry and Materials Science Composites Cytokines - metabolism Electrophoresis Fourier analysis Fourier transforms Gel electrophoresis Glass HaCaT Cells Hair Hair - chemistry Humans Hydrogels Hydrogels - chemistry In vivo methods and tests Inflammation Infrared analysis Infrared spectroscopy Keratin Keratins, Hair-Specific - chemistry Keratins, Hair-Specific - therapeutic use Leukocyte Count Materials Science Microscopy, Electron, Scanning Natural Materials Polyacrylamide Polymer Sciences Radiation Radiation effects Radiation injuries Radiation Injuries - therapy Rats Regeneration Regenerative Medicine/Tissue Engineering Repair Rheological properties Rheology Sodium Dodecyl Sulfate - chemistry Spectroscopy, Fourier Transform Infrared Sulfates Surfaces and Interfaces Thin Films Time Factors Tissue engineering Tissue Engineering Constructs and Cell Substrates Wound healing Wound Healing - drug effects |
title | Hair keratin promotes wound healing in rats with combined radiation-wound injury |
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