Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells
Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-m...
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| Veröffentlicht in: | Journal of Oleo Science 2020, Vol.69(3), pp.255-260 |
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| creator | Asai, Daisuke Kawano, Takahito Murata, Masaharu Nakashima, Hideki Toita, Riki Kang, Jeong-Hun |
| description | Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS. |
| doi_str_mv | 10.5650/jos.ess19268 |
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We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.</description><identifier>ISSN: 1345-8957</identifier><identifier>EISSN: 1347-3352</identifier><identifier>DOI: 10.5650/jos.ess19268</identifier><identifier>PMID: 32051357</identifier><language>eng</language><publisher>Japan: Japan Oil Chemists' Society</publisher><subject>Activation ; Aorta ; Apoptosis ; ERK1/2 ; Kinases ; lysophosphatidylcholine ; Muscles ; Phospholipase ; Phosphorylation ; proliferation ; Smooth muscle ; vascular smooth muscle cell ; Viability</subject><ispartof>Journal of Oleo Science, 2020, Vol.69(3), pp.255-260</ispartof><rights>2020 by Japan Oil Chemists' Society</rights><rights>Copyright Japan Science and Technology Agency 2020</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c654t-3cbf539ba63bbf3c3be83721d3214ee925f14bd935703a3ef0a3c87ac057192f3</citedby><cites>FETCH-LOGICAL-c654t-3cbf539ba63bbf3c3be83721d3214ee925f14bd935703a3ef0a3c87ac057192f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32051357$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Asai, Daisuke</creatorcontrib><creatorcontrib>Kawano, Takahito</creatorcontrib><creatorcontrib>Murata, Masaharu</creatorcontrib><creatorcontrib>Nakashima, Hideki</creatorcontrib><creatorcontrib>Toita, Riki</creatorcontrib><creatorcontrib>Kang, Jeong-Hun</creatorcontrib><creatorcontrib>Biomedical Research Institute</creatorcontrib><creatorcontrib>Kyushu University</creatorcontrib><creatorcontrib>Division of Biopharmaceutics and Pharmacokinetics</creatorcontrib><creatorcontrib>National Cerebral and Cardiovascular Center Research Institute</creatorcontrib><creatorcontrib>Department of Microbiology</creatorcontrib><creatorcontrib>AIST-OsakaUniversity Advanced Photonics and Biosensing Open Innovation Laboratory</creatorcontrib><creatorcontrib>AIST</creatorcontrib><creatorcontrib>Center for Advanced Medical Innovation</creatorcontrib><creatorcontrib>National Institute of Advanced Industrial Science and Technology (AIST</creatorcontrib><creatorcontrib>St. Marianna University School of Medicine</creatorcontrib><title>Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells</title><title>Journal of Oleo Science</title><addtitle>J Oleo Sci</addtitle><description>Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.</description><subject>Activation</subject><subject>Aorta</subject><subject>Apoptosis</subject><subject>ERK1/2</subject><subject>Kinases</subject><subject>lysophosphatidylcholine</subject><subject>Muscles</subject><subject>Phospholipase</subject><subject>Phosphorylation</subject><subject>proliferation</subject><subject>Smooth muscle</subject><subject>vascular smooth muscle cell</subject><subject>Viability</subject><issn>1345-8957</issn><issn>1347-3352</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNpFkF2L1TAQhoso7ofeeS0Bb-2aZpq2uTx72C84orB6HdJ0YlvapiapcP6Ev9l0z4cQZsLknXcmT5J8yOgNLzj90lt_g95nghXVq-Qyg7xMATh7_XLnaSV4eZFced9TGuu8fJtcAKM8A15eJn_vjEEdiDXkHoMayK39001IntEtI9naSeMUnAqdnUg8u723c2v93MZSsx90a4coT0dsOhWwId9dLBg8dqipIZvZzsH6zq8zHpdRTWRjXeg0eR6tDS35ung9INniMPh3yRujBo_vj_k6-Xl_92P7mO6-PTxtN7tUFzwPKejacBC1KqCuDWiosYKSZQ2wLEcUjJssrxsRv0hBARqqQFel0pSXEZSB6-TTwXd29veCPsjeLm6KIyWDQvBCiFxE1eeDSjvrvUMjZ9eNyu1lRuUKP3Z5eYIf5R-PpksdgZzFJ9pR8HAQrLi0Guy0wvs_uulLO2C0ZJRRSWkhKMQkJGWcx1BQyKu84kV0uj049T6oX3gepVawA77sVQgJazjtd37UrXISJ_gHmMOyNw</recordid><startdate>20200101</startdate><enddate>20200101</enddate><creator>Asai, Daisuke</creator><creator>Kawano, Takahito</creator><creator>Murata, Masaharu</creator><creator>Nakashima, Hideki</creator><creator>Toita, Riki</creator><creator>Kang, Jeong-Hun</creator><general>Japan Oil Chemists' Society</general><general>Japan Science and Technology Agency</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope></search><sort><creationdate>20200101</creationdate><title>Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells</title><author>Asai, Daisuke ; Kawano, Takahito ; Murata, Masaharu ; Nakashima, Hideki ; Toita, Riki ; Kang, Jeong-Hun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c654t-3cbf539ba63bbf3c3be83721d3214ee925f14bd935703a3ef0a3c87ac057192f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Activation</topic><topic>Aorta</topic><topic>Apoptosis</topic><topic>ERK1/2</topic><topic>Kinases</topic><topic>lysophosphatidylcholine</topic><topic>Muscles</topic><topic>Phospholipase</topic><topic>Phosphorylation</topic><topic>proliferation</topic><topic>Smooth muscle</topic><topic>vascular smooth muscle cell</topic><topic>Viability</topic><toplevel>online_resources</toplevel><creatorcontrib>Asai, Daisuke</creatorcontrib><creatorcontrib>Kawano, Takahito</creatorcontrib><creatorcontrib>Murata, Masaharu</creatorcontrib><creatorcontrib>Nakashima, Hideki</creatorcontrib><creatorcontrib>Toita, Riki</creatorcontrib><creatorcontrib>Kang, Jeong-Hun</creatorcontrib><creatorcontrib>Biomedical Research Institute</creatorcontrib><creatorcontrib>Kyushu University</creatorcontrib><creatorcontrib>Division of Biopharmaceutics and Pharmacokinetics</creatorcontrib><creatorcontrib>National Cerebral and Cardiovascular Center Research Institute</creatorcontrib><creatorcontrib>Department of Microbiology</creatorcontrib><creatorcontrib>AIST-OsakaUniversity Advanced Photonics and Biosensing Open Innovation Laboratory</creatorcontrib><creatorcontrib>AIST</creatorcontrib><creatorcontrib>Center for Advanced Medical Innovation</creatorcontrib><creatorcontrib>National Institute of Advanced Industrial Science and Technology (AIST</creatorcontrib><creatorcontrib>St. Marianna University School of Medicine</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><jtitle>Journal of Oleo Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Asai, Daisuke</au><au>Kawano, Takahito</au><au>Murata, Masaharu</au><au>Nakashima, Hideki</au><au>Toita, Riki</au><au>Kang, Jeong-Hun</au><aucorp>Biomedical Research Institute</aucorp><aucorp>Kyushu University</aucorp><aucorp>Division of Biopharmaceutics and Pharmacokinetics</aucorp><aucorp>National Cerebral and Cardiovascular Center Research Institute</aucorp><aucorp>Department of Microbiology</aucorp><aucorp>AIST-OsakaUniversity Advanced Photonics and Biosensing Open Innovation Laboratory</aucorp><aucorp>AIST</aucorp><aucorp>Center for Advanced Medical Innovation</aucorp><aucorp>National Institute of Advanced Industrial Science and Technology (AIST</aucorp><aucorp>St. Marianna University School of Medicine</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells</atitle><jtitle>Journal of Oleo Science</jtitle><addtitle>J Oleo Sci</addtitle><date>2020-01-01</date><risdate>2020</risdate><volume>69</volume><issue>3</issue><spage>255</spage><epage>260</epage><pages>255-260</pages><issn>1345-8957</issn><eissn>1347-3352</eissn><abstract>Lysophosphatidylcholine (lysoPtdCho) is produced by the phospholipase A2-mediated hydrolysis of phosphatidylcholine and can stimulate proliferation and apoptosis of vascular smooth muscle cells. We examined the influence of fetal bovine serum (FBS) concentration in the culture medium on lysoPtdCho-mediated apoptosis and proliferation of human aortic smooth muscle cells (HASMCs) as well as on the activation of extracellular signal-regulated kinases (ERK)1/2. In the presence of 1% FBS, HASMC viability increased after lysoPtdCho treatment at 1 and 10 μM but decreased at 25 and 50 μM. However, lysoPtdCho increased HASMC viability in a dose-dependent manner in the presence of 10% FBS. The activity of caspase 3/7 in HASMCs was increased by 25 μM lysoPtdCho in the presence of 1% FBS, but not 10% FBS. Furthermore, lysoPtdCho at 1 and 10 μM triggered ERK1/2 phosphorylation in the presence of 1% FBS, but not at 10% FBS. Thus, lysoPtdCho-mediated HASMC apoptosis, proliferation, and ERK1/2 activation are dependent on the concentration of FBS.</abstract><cop>Japan</cop><pub>Japan Oil Chemists' Society</pub><pmid>32051357</pmid><doi>10.5650/jos.ess19268</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Activation Aorta Apoptosis ERK1/2 Kinases lysophosphatidylcholine Muscles Phospholipase Phosphorylation proliferation Smooth muscle vascular smooth muscle cell Viability |
| title | Effect of Fetal Bovine Serum Concentration on Lysophosphatidylcholine-mediated Proliferation and Apoptosis of Human Aortic Smooth Muscle Cells |
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