The label-free separation and culture of tumor cells in a microfluidic biochip
Circulating tumor cells (CTCs) from liquid biopsy have shown a strong correlation to the clinical outcome of cancer patients. The enumeration and cytological analysis of CTCs have attracted increasing efforts for cancer disease management amid immunotherapy and personalized medicine. However, both e...
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Veröffentlicht in: | Analyst (London) 2020-03, Vol.145 (5), p.176-1715 |
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creator | Zhou, Jian Tu, Chunlong Liang, Yitao Huang, Bobo Fang, Yifeng Liang, Xiao Ye, Xuesong |
description | Circulating tumor cells (CTCs) from liquid biopsy have shown a strong correlation to the clinical outcome of cancer patients. The enumeration and cytological analysis of CTCs have attracted increasing efforts for cancer disease management amid immunotherapy and personalized medicine. However, both enumeration and cytological analysis are challenging due to the rarity of CTCs and the lack of integrated solutions for the minimal risk of cell loss in the course of CTC procurement. We report a simple microfluidic chip permitting a one-stop solution for streamlining the on-chip cell separation, capture, immunofluorescence assay and/or
in situ
culture of isolated cells devoid of risky manual steps. Our results showed effective trapping of single cells, doublets and cell lumps isolated from blood in the same device. On-chip immunostaining revealed normal cell morphology and the characterization of cell expansion uncovered an altered cell growth curve with a reduced lag phase as compared to the conventional culture despite closely matching cell growth rates. The cells were viable and functional for as long as 11 days inside our chip and cell migration was also readily observed, with lumps showing greater aggressiveness than single cells. With these results, we expect promising applications of our one-stop solution for liquid biopsy
via
CTCs.
An integrated microfluidic biochip was designed for the streamlined separation, capture,
in situ
culture and/or immunofluorescence characterization of tumor cells. |
doi_str_mv | 10.1039/c9an02092f |
format | Article |
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in situ
culture of isolated cells devoid of risky manual steps. Our results showed effective trapping of single cells, doublets and cell lumps isolated from blood in the same device. On-chip immunostaining revealed normal cell morphology and the characterization of cell expansion uncovered an altered cell growth curve with a reduced lag phase as compared to the conventional culture despite closely matching cell growth rates. The cells were viable and functional for as long as 11 days inside our chip and cell migration was also readily observed, with lumps showing greater aggressiveness than single cells. With these results, we expect promising applications of our one-stop solution for liquid biopsy
via
CTCs.
An integrated microfluidic biochip was designed for the streamlined separation, capture,
in situ
culture and/or immunofluorescence characterization of tumor cells.</description><identifier>ISSN: 0003-2654</identifier><identifier>EISSN: 1364-5528</identifier><identifier>DOI: 10.1039/c9an02092f</identifier><identifier>PMID: 31895371</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Biochips ; Biopsy ; Cancer ; Cell adhesion & migration ; Cell growth ; Disease control ; Enumeration ; Immunofluorescence ; Microfluidics ; Morphology ; Procurement ; Separation ; Streamlining ; Tumors</subject><ispartof>Analyst (London), 2020-03, Vol.145 (5), p.176-1715</ispartof><rights>Copyright Royal Society of Chemistry 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c429t-c198c818a4c6b8b8c374a4b17215d78d1fcbf80537cad19e3ee44df8e46948423</citedby><cites>FETCH-LOGICAL-c429t-c198c818a4c6b8b8c374a4b17215d78d1fcbf80537cad19e3ee44df8e46948423</cites><orcidid>0000-0002-3439-3733 ; 0000-0002-8676-8891</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,2831,2832,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31895371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Tu, Chunlong</creatorcontrib><creatorcontrib>Liang, Yitao</creatorcontrib><creatorcontrib>Huang, Bobo</creatorcontrib><creatorcontrib>Fang, Yifeng</creatorcontrib><creatorcontrib>Liang, Xiao</creatorcontrib><creatorcontrib>Ye, Xuesong</creatorcontrib><title>The label-free separation and culture of tumor cells in a microfluidic biochip</title><title>Analyst (London)</title><addtitle>Analyst</addtitle><description>Circulating tumor cells (CTCs) from liquid biopsy have shown a strong correlation to the clinical outcome of cancer patients. The enumeration and cytological analysis of CTCs have attracted increasing efforts for cancer disease management amid immunotherapy and personalized medicine. However, both enumeration and cytological analysis are challenging due to the rarity of CTCs and the lack of integrated solutions for the minimal risk of cell loss in the course of CTC procurement. We report a simple microfluidic chip permitting a one-stop solution for streamlining the on-chip cell separation, capture, immunofluorescence assay and/or
in situ
culture of isolated cells devoid of risky manual steps. Our results showed effective trapping of single cells, doublets and cell lumps isolated from blood in the same device. On-chip immunostaining revealed normal cell morphology and the characterization of cell expansion uncovered an altered cell growth curve with a reduced lag phase as compared to the conventional culture despite closely matching cell growth rates. The cells were viable and functional for as long as 11 days inside our chip and cell migration was also readily observed, with lumps showing greater aggressiveness than single cells. With these results, we expect promising applications of our one-stop solution for liquid biopsy
via
CTCs.
An integrated microfluidic biochip was designed for the streamlined separation, capture,
in situ
culture and/or immunofluorescence characterization of tumor cells.</description><subject>Biochips</subject><subject>Biopsy</subject><subject>Cancer</subject><subject>Cell adhesion & migration</subject><subject>Cell growth</subject><subject>Disease control</subject><subject>Enumeration</subject><subject>Immunofluorescence</subject><subject>Microfluidics</subject><subject>Morphology</subject><subject>Procurement</subject><subject>Separation</subject><subject>Streamlining</subject><subject>Tumors</subject><issn>0003-2654</issn><issn>1364-5528</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp90b1LAzEYBvAgiq3VxV2JuIhwmq-7S8ZSrAqlLnU-crmEptyXyWXwvze1tYKDUwjvj4c3TwC4xOgBIyoelZAtIkgQcwTGmGYsSVPCj8EYIUQTkqVsBM6838QrRik6BSOKuUhpjsdguVprWMtS14lxWkOve-nkYLsWyraCKtRDcBp2Bg6h6RxUuq49tHEKG6tcZ-pgK6tgaTu1tv05ODGy9vpif07A-_xpNXtJFm_Pr7PpIlGMiCFRWHDFMZdMZSUvuaI5k6zEOcFplfMKG1UajuKKSlZYaKo1Y5XhmmWCcUboBNztcnvXfQTth6KxfrubbHUXfEEoJYhjRrf09g_ddMG1cbuoYlzsCOGo7ncqvsl7p03RO9tI91lgVGxbLmZiuvxueR7x9T4ylI2uDvSn1ghudsB5dZj-flPRVyaaq_8M_QKXSYtH</recordid><startdate>20200302</startdate><enddate>20200302</enddate><creator>Zhou, Jian</creator><creator>Tu, Chunlong</creator><creator>Liang, Yitao</creator><creator>Huang, Bobo</creator><creator>Fang, Yifeng</creator><creator>Liang, Xiao</creator><creator>Ye, Xuesong</creator><general>Royal Society of Chemistry</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3439-3733</orcidid><orcidid>https://orcid.org/0000-0002-8676-8891</orcidid></search><sort><creationdate>20200302</creationdate><title>The label-free separation and culture of tumor cells in a microfluidic biochip</title><author>Zhou, Jian ; Tu, Chunlong ; Liang, Yitao ; Huang, Bobo ; Fang, Yifeng ; Liang, Xiao ; Ye, Xuesong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c429t-c198c818a4c6b8b8c374a4b17215d78d1fcbf80537cad19e3ee44df8e46948423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biochips</topic><topic>Biopsy</topic><topic>Cancer</topic><topic>Cell adhesion & migration</topic><topic>Cell growth</topic><topic>Disease control</topic><topic>Enumeration</topic><topic>Immunofluorescence</topic><topic>Microfluidics</topic><topic>Morphology</topic><topic>Procurement</topic><topic>Separation</topic><topic>Streamlining</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Jian</creatorcontrib><creatorcontrib>Tu, Chunlong</creatorcontrib><creatorcontrib>Liang, Yitao</creatorcontrib><creatorcontrib>Huang, Bobo</creatorcontrib><creatorcontrib>Fang, Yifeng</creatorcontrib><creatorcontrib>Liang, Xiao</creatorcontrib><creatorcontrib>Ye, Xuesong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analyst (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Jian</au><au>Tu, Chunlong</au><au>Liang, Yitao</au><au>Huang, Bobo</au><au>Fang, Yifeng</au><au>Liang, Xiao</au><au>Ye, Xuesong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The label-free separation and culture of tumor cells in a microfluidic biochip</atitle><jtitle>Analyst (London)</jtitle><addtitle>Analyst</addtitle><date>2020-03-02</date><risdate>2020</risdate><volume>145</volume><issue>5</issue><spage>176</spage><epage>1715</epage><pages>176-1715</pages><issn>0003-2654</issn><eissn>1364-5528</eissn><abstract>Circulating tumor cells (CTCs) from liquid biopsy have shown a strong correlation to the clinical outcome of cancer patients. The enumeration and cytological analysis of CTCs have attracted increasing efforts for cancer disease management amid immunotherapy and personalized medicine. However, both enumeration and cytological analysis are challenging due to the rarity of CTCs and the lack of integrated solutions for the minimal risk of cell loss in the course of CTC procurement. We report a simple microfluidic chip permitting a one-stop solution for streamlining the on-chip cell separation, capture, immunofluorescence assay and/or
in situ
culture of isolated cells devoid of risky manual steps. Our results showed effective trapping of single cells, doublets and cell lumps isolated from blood in the same device. On-chip immunostaining revealed normal cell morphology and the characterization of cell expansion uncovered an altered cell growth curve with a reduced lag phase as compared to the conventional culture despite closely matching cell growth rates. The cells were viable and functional for as long as 11 days inside our chip and cell migration was also readily observed, with lumps showing greater aggressiveness than single cells. With these results, we expect promising applications of our one-stop solution for liquid biopsy
via
CTCs.
An integrated microfluidic biochip was designed for the streamlined separation, capture,
in situ
culture and/or immunofluorescence characterization of tumor cells.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>31895371</pmid><doi>10.1039/c9an02092f</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-3439-3733</orcidid><orcidid>https://orcid.org/0000-0002-8676-8891</orcidid></addata></record> |
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source | Royal Society Of Chemistry Journals; Royal Society of Chemistry Journals Archive (1841-2007); Alma/SFX Local Collection |
subjects | Biochips Biopsy Cancer Cell adhesion & migration Cell growth Disease control Enumeration Immunofluorescence Microfluidics Morphology Procurement Separation Streamlining Tumors |
title | The label-free separation and culture of tumor cells in a microfluidic biochip |
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