Expression of flagellin at yeast surface increases biocontrol efficiency of yeast cells against postharvest disease of tomato caused by Botrytis cinerea
Yeast expressing flagellin at cell surface could induce resistance in tomato fruit. [Display omitted] •Yeast expressing flagellin induce disease resistance against B. cinerea in tomato.•Yeast expressing flagellin induce expression of defense-related genes.•Yeast expressing flagellin induce superoxid...
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| Veröffentlicht in: | Postharvest biology and technology 2020-04, Vol.162, p.111112, Article 111112 |
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| container_title | Postharvest biology and technology |
| container_volume | 162 |
| creator | Zhao, Shixue Guo, Yanhua Wang, Qiannan Luo, Hongli He, Chaozu An, Bang |
| description | Yeast expressing flagellin at cell surface could induce resistance in tomato fruit.
[Display omitted]
•Yeast expressing flagellin induce disease resistance against B. cinerea in tomato.•Yeast expressing flagellin induce expression of defense-related genes.•Yeast expressing flagellin induce superoxide radical generation.
In this study, bacterial flagellin was expressed at the cell surface of Saccharomyces cerevisiae EBY100 via yeast surface display system. The effect of the yeast on controlling the postharvest decay of tomato fruit caused by Botrytis cinerea and the possible mechanisms were investigated. The results showed that the yeast expressing flagellin at cell surface could significantly induce disease resistance against B. cinerea in tomato wounds. The genes involved in biosynthesis of salicylic acid and jasmonic acid, and plant defense were markedly up-regulated in tomato wounds by the yeast strain expressing flagellin. In addition, application of the yeast strain significantly induced the superoxide radical generation in tobacco leaves and tomato fruit. These findings suggest that expressing flagellin at yeast cell surface may be an effective strategy to increase the biocontrol efficiency of antagonist yeast against disease in postharvest fruit. |
| doi_str_mv | 10.1016/j.postharvbio.2019.111112 |
| format | Article |
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[Display omitted]
•Yeast expressing flagellin induce disease resistance against B. cinerea in tomato.•Yeast expressing flagellin induce expression of defense-related genes.•Yeast expressing flagellin induce superoxide radical generation.
In this study, bacterial flagellin was expressed at the cell surface of Saccharomyces cerevisiae EBY100 via yeast surface display system. The effect of the yeast on controlling the postharvest decay of tomato fruit caused by Botrytis cinerea and the possible mechanisms were investigated. The results showed that the yeast expressing flagellin at cell surface could significantly induce disease resistance against B. cinerea in tomato wounds. The genes involved in biosynthesis of salicylic acid and jasmonic acid, and plant defense were markedly up-regulated in tomato wounds by the yeast strain expressing flagellin. In addition, application of the yeast strain significantly induced the superoxide radical generation in tobacco leaves and tomato fruit. These findings suggest that expressing flagellin at yeast cell surface may be an effective strategy to increase the biocontrol efficiency of antagonist yeast against disease in postharvest fruit.</description><identifier>ISSN: 0925-5214</identifier><identifier>EISSN: 1873-2356</identifier><identifier>DOI: 10.1016/j.postharvbio.2019.111112</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological control ; Biosynthesis ; Botrytis cinerea ; Cell surface ; Disease resistance ; Display devices ; Flagellin ; Fruits ; Induced resistance ; Jasmonic acid ; Leaves ; Post-harvest decay ; Salicylic acid ; Superoxide ; Tobacco ; Tomato ; Tomatoes ; Wounds ; Yeast ; Yeast surface display ; Yeasts</subject><ispartof>Postharvest biology and technology, 2020-04, Vol.162, p.111112, Article 111112</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright Elsevier BV Apr 2020</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-993dcafebd1347ce1dc4b98839f28c691a3abfe8cdba60dc7d2366ce1cbdec273</citedby><cites>FETCH-LOGICAL-c415t-993dcafebd1347ce1dc4b98839f28c691a3abfe8cdba60dc7d2366ce1cbdec273</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925521419310622$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Zhao, Shixue</creatorcontrib><creatorcontrib>Guo, Yanhua</creatorcontrib><creatorcontrib>Wang, Qiannan</creatorcontrib><creatorcontrib>Luo, Hongli</creatorcontrib><creatorcontrib>He, Chaozu</creatorcontrib><creatorcontrib>An, Bang</creatorcontrib><title>Expression of flagellin at yeast surface increases biocontrol efficiency of yeast cells against postharvest disease of tomato caused by Botrytis cinerea</title><title>Postharvest biology and technology</title><description>Yeast expressing flagellin at cell surface could induce resistance in tomato fruit.
[Display omitted]
•Yeast expressing flagellin induce disease resistance against B. cinerea in tomato.•Yeast expressing flagellin induce expression of defense-related genes.•Yeast expressing flagellin induce superoxide radical generation.
In this study, bacterial flagellin was expressed at the cell surface of Saccharomyces cerevisiae EBY100 via yeast surface display system. The effect of the yeast on controlling the postharvest decay of tomato fruit caused by Botrytis cinerea and the possible mechanisms were investigated. The results showed that the yeast expressing flagellin at cell surface could significantly induce disease resistance against B. cinerea in tomato wounds. The genes involved in biosynthesis of salicylic acid and jasmonic acid, and plant defense were markedly up-regulated in tomato wounds by the yeast strain expressing flagellin. In addition, application of the yeast strain significantly induced the superoxide radical generation in tobacco leaves and tomato fruit. These findings suggest that expressing flagellin at yeast cell surface may be an effective strategy to increase the biocontrol efficiency of antagonist yeast against disease in postharvest fruit.</description><subject>Biological control</subject><subject>Biosynthesis</subject><subject>Botrytis cinerea</subject><subject>Cell surface</subject><subject>Disease resistance</subject><subject>Display devices</subject><subject>Flagellin</subject><subject>Fruits</subject><subject>Induced resistance</subject><subject>Jasmonic acid</subject><subject>Leaves</subject><subject>Post-harvest decay</subject><subject>Salicylic acid</subject><subject>Superoxide</subject><subject>Tobacco</subject><subject>Tomato</subject><subject>Tomatoes</subject><subject>Wounds</subject><subject>Yeast</subject><subject>Yeast surface display</subject><subject>Yeasts</subject><issn>0925-5214</issn><issn>1873-2356</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqNUctOLCEQJUYT547-A8Z1j9BMP1h6J141MXGja0IXhTJpmxEYY_-JnyttXxOXsoGqnEcVh5Azzlac8fpiu9r5mJ51eOucX5WMyxWfTnlAFrxtRFGKqj4kCybLqqhKvj4mf2LcMsaqqmoX5OPqfRcwRucH6i21vX7CvncD1YmOqGOicR-sBqRugJAbGGl2Aj-k4HuK1jpwOMA4sWcCZIFI9ZN2Q66-x8P8Ni5OChM0-RedPAW9j2hoN9K_PoUxuUjBDZiNTsiR1X3E0__3kjz-u3rY3BR399e3m8u7Ata8SoWUwoC22Bku1g0gN7DuZNsKacsWasm10J3FFkyna2agMaWo64yDziCUjViS81l3F_zrPk-ptn4fhmypMlIKwRreZpScURB8jAGt2gX3osOoOFNTEGqrfgShpiDUHETmbmYu5jXeHAYVv74MjQsISRnvfqHyCQlunZo</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Zhao, Shixue</creator><creator>Guo, Yanhua</creator><creator>Wang, Qiannan</creator><creator>Luo, Hongli</creator><creator>He, Chaozu</creator><creator>An, Bang</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope></search><sort><creationdate>202004</creationdate><title>Expression of flagellin at yeast surface increases biocontrol efficiency of yeast cells against postharvest disease of tomato caused by Botrytis cinerea</title><author>Zhao, Shixue ; Guo, Yanhua ; Wang, Qiannan ; Luo, Hongli ; He, Chaozu ; An, Bang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-993dcafebd1347ce1dc4b98839f28c691a3abfe8cdba60dc7d2366ce1cbdec273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biological control</topic><topic>Biosynthesis</topic><topic>Botrytis cinerea</topic><topic>Cell surface</topic><topic>Disease resistance</topic><topic>Display devices</topic><topic>Flagellin</topic><topic>Fruits</topic><topic>Induced resistance</topic><topic>Jasmonic acid</topic><topic>Leaves</topic><topic>Post-harvest decay</topic><topic>Salicylic acid</topic><topic>Superoxide</topic><topic>Tobacco</topic><topic>Tomato</topic><topic>Tomatoes</topic><topic>Wounds</topic><topic>Yeast</topic><topic>Yeast surface display</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Shixue</creatorcontrib><creatorcontrib>Guo, Yanhua</creatorcontrib><creatorcontrib>Wang, Qiannan</creatorcontrib><creatorcontrib>Luo, Hongli</creatorcontrib><creatorcontrib>He, Chaozu</creatorcontrib><creatorcontrib>An, Bang</creatorcontrib><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Postharvest biology and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Shixue</au><au>Guo, Yanhua</au><au>Wang, Qiannan</au><au>Luo, Hongli</au><au>He, Chaozu</au><au>An, Bang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of flagellin at yeast surface increases biocontrol efficiency of yeast cells against postharvest disease of tomato caused by Botrytis cinerea</atitle><jtitle>Postharvest biology and technology</jtitle><date>2020-04</date><risdate>2020</risdate><volume>162</volume><spage>111112</spage><pages>111112-</pages><artnum>111112</artnum><issn>0925-5214</issn><eissn>1873-2356</eissn><abstract>Yeast expressing flagellin at cell surface could induce resistance in tomato fruit.
[Display omitted]
•Yeast expressing flagellin induce disease resistance against B. cinerea in tomato.•Yeast expressing flagellin induce expression of defense-related genes.•Yeast expressing flagellin induce superoxide radical generation.
In this study, bacterial flagellin was expressed at the cell surface of Saccharomyces cerevisiae EBY100 via yeast surface display system. The effect of the yeast on controlling the postharvest decay of tomato fruit caused by Botrytis cinerea and the possible mechanisms were investigated. The results showed that the yeast expressing flagellin at cell surface could significantly induce disease resistance against B. cinerea in tomato wounds. The genes involved in biosynthesis of salicylic acid and jasmonic acid, and plant defense were markedly up-regulated in tomato wounds by the yeast strain expressing flagellin. In addition, application of the yeast strain significantly induced the superoxide radical generation in tobacco leaves and tomato fruit. These findings suggest that expressing flagellin at yeast cell surface may be an effective strategy to increase the biocontrol efficiency of antagonist yeast against disease in postharvest fruit.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/j.postharvbio.2019.111112</doi></addata></record> |
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| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biological control Biosynthesis Botrytis cinerea Cell surface Disease resistance Display devices Flagellin Fruits Induced resistance Jasmonic acid Leaves Post-harvest decay Salicylic acid Superoxide Tobacco Tomato Tomatoes Wounds Yeast Yeast surface display Yeasts |
| title | Expression of flagellin at yeast surface increases biocontrol efficiency of yeast cells against postharvest disease of tomato caused by Botrytis cinerea |
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