EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia
BACKGROUND Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and...
Gespeichert in:
| Veröffentlicht in: | Pest management science 2020-04, Vol.76 (4), p.1214-1221 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1221 |
|---|---|
| container_issue | 4 |
| container_start_page | 1214 |
| container_title | Pest management science |
| container_volume | 76 |
| creator | Adu‐Yeboah, Patricia Malone, Jenna M Fleet, Benjamin Gill, Gurjeet Preston, Christopher |
| description | BACKGROUND
Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and to determine resistance mechanisms.
RESULTS
Dose–response experiments confirmed resistance of these populations to glyphosate with resistance levels of 2.8–6.6‐fold compared with two susceptible populations. Shikimate assays confirmed resistance to glyphosate with less shikimate accumulation in the resistant populations compared with the susceptible populations. Quantitative PCR of genomic DNA showed increased gene copy number in the resistant populations with 9–11‐fold more copies of EPSPS compared with the susceptible populations, suggesting resistance is likely conferred by gene amplification.
CONCLUSION
This study identified the first examples of glyphosate resistance in the grass species H. glaucum with resistance associated with EPSPS gene amplification. © 2019 Society of Chemical Industry
Glyphosate failed to control some populations of Hordeum glaucum along fence lines in South Australia. This study identified EPSPS gene amplification as the mechanism conferring resistance in these populations. |
| doi_str_mv | 10.1002/ps.5671 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2369204462</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2369204462</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3451-d988498bbb50cbd345d4ce6c70b40d47111b9e66772a4d81224fc6efc195ab3d3</originalsourceid><addsrcrecordid>eNp1kNtq3DAQhkVpaU6lbxAEvWhD2VSSZdm6XEJOsJAFt9A7o8N418FrOTpQ9jnywtVmk73L1T_MfPMP8yP0lZJLSgj7NYXLUlT0AzqmJRMzLmX98VDXf4_QSQiPhBApJfuMjgoqasGL8hg9Xy-bZYNXMAJWm2nou96o2LsRGzd24AP2EPoQ1WgAR4dXw3Zau6AiHAYRT25Kw8tWwK7Dd85bSJvMqmSyNjGBxT9G5-Ma_Ii18gNs8cqrEC5wP-LGpbjG8xSiV0OvztCnTg0BvrzqKfpzc_376m62eLi9v5ovZqbgJZ1ZWdf5Oa11SYy2uWe5AWEqojmxvKKUaglCVBVT3NaUMd4ZAZ2hslS6sMUp-rb3nbx7ShBi--iSH_PJlhVCMsK5YJn6vqeMdyF46NrJ9xvlty0l7S78dgrtLvxMnr_6Jb0Be-De0s7Azz3wr88BvOfTLpsXu_9IgY-m</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2369204462</pqid></control><display><type>article</type><title>EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia</title><source>MEDLINE</source><source>Wiley Online Library</source><creator>Adu‐Yeboah, Patricia ; Malone, Jenna M ; Fleet, Benjamin ; Gill, Gurjeet ; Preston, Christopher</creator><creatorcontrib>Adu‐Yeboah, Patricia ; Malone, Jenna M ; Fleet, Benjamin ; Gill, Gurjeet ; Preston, Christopher</creatorcontrib><description>BACKGROUND
Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and to determine resistance mechanisms.
RESULTS
Dose–response experiments confirmed resistance of these populations to glyphosate with resistance levels of 2.8–6.6‐fold compared with two susceptible populations. Shikimate assays confirmed resistance to glyphosate with less shikimate accumulation in the resistant populations compared with the susceptible populations. Quantitative PCR of genomic DNA showed increased gene copy number in the resistant populations with 9–11‐fold more copies of EPSPS compared with the susceptible populations, suggesting resistance is likely conferred by gene amplification.
CONCLUSION
This study identified the first examples of glyphosate resistance in the grass species H. glaucum with resistance associated with EPSPS gene amplification. © 2019 Society of Chemical Industry
Glyphosate failed to control some populations of Hordeum glaucum along fence lines in South Australia. This study identified EPSPS gene amplification as the mechanism conferring resistance in these populations.</description><identifier>ISSN: 1526-498X</identifier><identifier>EISSN: 1526-4998</identifier><identifier>DOI: 10.1002/ps.5671</identifier><identifier>PMID: 31686435</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Amplification ; Barley ; Copy number ; Deoxyribonucleic acid ; DNA ; EPSPS ; EPSPS gene ; Gene Amplification ; Glycine - analogs & derivatives ; Glyphosate ; glyphosate resistance ; Grasses ; Herbicide Resistance ; Herbicides ; Hordeum ; Hordeum glaucum ; Organic chemistry ; Populations ; South Australia</subject><ispartof>Pest management science, 2020-04, Vol.76 (4), p.1214-1221</ispartof><rights>2019 Society of Chemical Industry</rights><rights>2019 Society of Chemical Industry.</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3451-d988498bbb50cbd345d4ce6c70b40d47111b9e66772a4d81224fc6efc195ab3d3</citedby><cites>FETCH-LOGICAL-c3451-d988498bbb50cbd345d4ce6c70b40d47111b9e66772a4d81224fc6efc195ab3d3</cites><orcidid>0000-0001-8285-8139</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fps.5671$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fps.5671$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31686435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adu‐Yeboah, Patricia</creatorcontrib><creatorcontrib>Malone, Jenna M</creatorcontrib><creatorcontrib>Fleet, Benjamin</creatorcontrib><creatorcontrib>Gill, Gurjeet</creatorcontrib><creatorcontrib>Preston, Christopher</creatorcontrib><title>EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia</title><title>Pest management science</title><addtitle>Pest Manag Sci</addtitle><description>BACKGROUND
Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and to determine resistance mechanisms.
RESULTS
Dose–response experiments confirmed resistance of these populations to glyphosate with resistance levels of 2.8–6.6‐fold compared with two susceptible populations. Shikimate assays confirmed resistance to glyphosate with less shikimate accumulation in the resistant populations compared with the susceptible populations. Quantitative PCR of genomic DNA showed increased gene copy number in the resistant populations with 9–11‐fold more copies of EPSPS compared with the susceptible populations, suggesting resistance is likely conferred by gene amplification.
CONCLUSION
This study identified the first examples of glyphosate resistance in the grass species H. glaucum with resistance associated with EPSPS gene amplification. © 2019 Society of Chemical Industry
Glyphosate failed to control some populations of Hordeum glaucum along fence lines in South Australia. This study identified EPSPS gene amplification as the mechanism conferring resistance in these populations.</description><subject>Amplification</subject><subject>Barley</subject><subject>Copy number</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>EPSPS</subject><subject>EPSPS gene</subject><subject>Gene Amplification</subject><subject>Glycine - analogs & derivatives</subject><subject>Glyphosate</subject><subject>glyphosate resistance</subject><subject>Grasses</subject><subject>Herbicide Resistance</subject><subject>Herbicides</subject><subject>Hordeum</subject><subject>Hordeum glaucum</subject><subject>Organic chemistry</subject><subject>Populations</subject><subject>South Australia</subject><issn>1526-498X</issn><issn>1526-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kNtq3DAQhkVpaU6lbxAEvWhD2VSSZdm6XEJOsJAFt9A7o8N418FrOTpQ9jnywtVmk73L1T_MfPMP8yP0lZJLSgj7NYXLUlT0AzqmJRMzLmX98VDXf4_QSQiPhBApJfuMjgoqasGL8hg9Xy-bZYNXMAJWm2nou96o2LsRGzd24AP2EPoQ1WgAR4dXw3Zau6AiHAYRT25Kw8tWwK7Dd85bSJvMqmSyNjGBxT9G5-Ma_Ii18gNs8cqrEC5wP-LGpbjG8xSiV0OvztCnTg0BvrzqKfpzc_376m62eLi9v5ovZqbgJZ1ZWdf5Oa11SYy2uWe5AWEqojmxvKKUaglCVBVT3NaUMd4ZAZ2hslS6sMUp-rb3nbx7ShBi--iSH_PJlhVCMsK5YJn6vqeMdyF46NrJ9xvlty0l7S78dgrtLvxMnr_6Jb0Be-De0s7Azz3wr88BvOfTLpsXu_9IgY-m</recordid><startdate>202004</startdate><enddate>202004</enddate><creator>Adu‐Yeboah, Patricia</creator><creator>Malone, Jenna M</creator><creator>Fleet, Benjamin</creator><creator>Gill, Gurjeet</creator><creator>Preston, Christopher</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0001-8285-8139</orcidid></search><sort><creationdate>202004</creationdate><title>EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia</title><author>Adu‐Yeboah, Patricia ; Malone, Jenna M ; Fleet, Benjamin ; Gill, Gurjeet ; Preston, Christopher</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3451-d988498bbb50cbd345d4ce6c70b40d47111b9e66772a4d81224fc6efc195ab3d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Amplification</topic><topic>Barley</topic><topic>Copy number</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>EPSPS</topic><topic>EPSPS gene</topic><topic>Gene Amplification</topic><topic>Glycine - analogs & derivatives</topic><topic>Glyphosate</topic><topic>glyphosate resistance</topic><topic>Grasses</topic><topic>Herbicide Resistance</topic><topic>Herbicides</topic><topic>Hordeum</topic><topic>Hordeum glaucum</topic><topic>Organic chemistry</topic><topic>Populations</topic><topic>South Australia</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adu‐Yeboah, Patricia</creatorcontrib><creatorcontrib>Malone, Jenna M</creatorcontrib><creatorcontrib>Fleet, Benjamin</creatorcontrib><creatorcontrib>Gill, Gurjeet</creatorcontrib><creatorcontrib>Preston, Christopher</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Pest management science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adu‐Yeboah, Patricia</au><au>Malone, Jenna M</au><au>Fleet, Benjamin</au><au>Gill, Gurjeet</au><au>Preston, Christopher</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia</atitle><jtitle>Pest management science</jtitle><addtitle>Pest Manag Sci</addtitle><date>2020-04</date><risdate>2020</risdate><volume>76</volume><issue>4</issue><spage>1214</spage><epage>1221</epage><pages>1214-1221</pages><issn>1526-498X</issn><eissn>1526-4998</eissn><abstract>BACKGROUND
Glyphosate failed to control two populations of Hordeum glaucum (northern barley grass) along a fence line and around stockyards near Arthurton on the Yorke Peninsula, South Australia after more than a decade of regular use of glyphosate. These were investigated to confirm resistance and to determine resistance mechanisms.
RESULTS
Dose–response experiments confirmed resistance of these populations to glyphosate with resistance levels of 2.8–6.6‐fold compared with two susceptible populations. Shikimate assays confirmed resistance to glyphosate with less shikimate accumulation in the resistant populations compared with the susceptible populations. Quantitative PCR of genomic DNA showed increased gene copy number in the resistant populations with 9–11‐fold more copies of EPSPS compared with the susceptible populations, suggesting resistance is likely conferred by gene amplification.
CONCLUSION
This study identified the first examples of glyphosate resistance in the grass species H. glaucum with resistance associated with EPSPS gene amplification. © 2019 Society of Chemical Industry
Glyphosate failed to control some populations of Hordeum glaucum along fence lines in South Australia. This study identified EPSPS gene amplification as the mechanism conferring resistance in these populations.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>31686435</pmid><doi>10.1002/ps.5671</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-8285-8139</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1526-498X |
| ispartof | Pest management science, 2020-04, Vol.76 (4), p.1214-1221 |
| issn | 1526-498X 1526-4998 |
| language | eng |
| recordid | cdi_proquest_journals_2369204462 |
| source | MEDLINE; Wiley Online Library |
| subjects | Amplification Barley Copy number Deoxyribonucleic acid DNA EPSPS EPSPS gene Gene Amplification Glycine - analogs & derivatives Glyphosate glyphosate resistance Grasses Herbicide Resistance Herbicides Hordeum Hordeum glaucum Organic chemistry Populations South Australia |
| title | EPSPS gene amplification confers resistance to glyphosate resistant populations of Hordeum glaucum Stued (northern barley grass) in South Australia |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T05%3A12%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=EPSPS%20gene%20amplification%20confers%20resistance%20to%20glyphosate%20resistant%20populations%20of%20Hordeum%20glaucum%20Stued%20(northern%20barley%20grass)%20in%20South%20Australia&rft.jtitle=Pest%20management%20science&rft.au=Adu%E2%80%90Yeboah,%20Patricia&rft.date=2020-04&rft.volume=76&rft.issue=4&rft.spage=1214&rft.epage=1221&rft.pages=1214-1221&rft.issn=1526-498X&rft.eissn=1526-4998&rft_id=info:doi/10.1002/ps.5671&rft_dat=%3Cproquest_cross%3E2369204462%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2369204462&rft_id=info:pmid/31686435&rfr_iscdi=true |