MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts
High temperature is an abiotic stress factor that threatens plant growth and development. Autophagy in response to heat stress involves the selective removal of heat-induced protein complexes. Previously, we showed that a crucial autophagy protein from apple, MdATG18a, has a positive effect on droug...
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| Veröffentlicht in: | Horticulture research 2020, Vol.7 (1), p.21, Article 21 |
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| creator | Huo, Liuqing Sun, Xun Guo, Zijian Jia, Xin Che, Runmin Sun, Yiming Zhu, Yanfei Wang, Ping Gong, Xiaoqing Ma, Fengwang |
| description | High temperature is an abiotic stress factor that threatens plant growth and development. Autophagy in response to heat stress involves the selective removal of heat-induced protein complexes. Previously, we showed that a crucial autophagy protein from apple, MdATG18a, has a positive effect on drought tolerance. In the present study, we treated transgenic apple (
Malus domestica
) plants overexpressing
MdATG18a
with high temperature and found that autophagy protected them from heat stress. Overexpression of
MdATG18a
in apple enhanced antioxidase activity and contributed to the production of increased beneficial antioxidants under heat stress. Transgenic apple plants exhibited higher photosynthetic capacity, as shown by the rate of CO
2
assimilation, the maximum photochemical efficiency of photosystem II (PSII), the effective quantum yield, and the electron transport rates in photosystems I and II (PSI and PSII, respectively). We also detected elevated autophagic activity and reduced damage to chloroplasts in transgenic plants compared to WT plants. In addition, the transcriptional activities of several
HSP
genes were increased in transgenic apple plants. In summary, we propose that autophagy plays a critical role in basal thermotolerance in apple, primarily through a combination of enhanced antioxidant activity and reduced chloroplast damage. |
| doi_str_mv | 10.1038/s41438-020-0243-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_journals_2368563964</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2368563964</sourcerecordid><originalsourceid>FETCH-LOGICAL-c502t-247413971dc040722ab5ff700053e7a3bc8b9b13ceab67e5db9b9eb1d51d20d23</originalsourceid><addsrcrecordid>eNp1UU1v1DAQtRCorZb-gF6QJc4p9tiOkwtSVUGLVMSlnC1_zGZTJXawsxX993i1pcCBgzXjmef3nvUIueDskjPRfSiSS9E1DFg9UjTwipwBU9Bo0O3r2rctNG3H2Sk5L-WBMcaVBKH0CTkVwCUDwc5I-hqu7m94Z2l6xIw_l4yljCnScV5yHRXqbLETXXeY57SmCbONHukY6Vq7MmAcPbXLMiF1TzSgz2jLGAca7GwHpGuifjelnJbJlrW8JW-2dip4_lw35PvnT_fXt83dt5sv11d3jVcM1gakllz0mgfPJNMA1qntVtdPKIHaCuc71zsuPFrXalSh3np0PCgegAUQG_LxyLvs3YzBY6x2J7Pkcbb5ySQ7mn83cdyZIT0azWQvqsqGvH8myOnHHstqHtI-x-rZgGg71Yq-lRXFjyifUykZty8KnJlDTOYYk6kxmUNM5mDt3d_WXl78DqUC4AgodRUHzH-k_8_6C52Nn80</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2368563964</pqid></control><display><type>article</type><title>MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts</title><source>SpringerOpen</source><source>Directory of Open Access Journals</source><source>Nature_OA刊</source><source>Oxford University Press Open Access</source><source>PubMed Central</source><source>EZB Electronic Journals Library</source><creator>Huo, Liuqing ; Sun, Xun ; Guo, Zijian ; Jia, Xin ; Che, Runmin ; Sun, Yiming ; Zhu, Yanfei ; Wang, Ping ; Gong, Xiaoqing ; Ma, Fengwang</creator><creatorcontrib>Huo, Liuqing ; Sun, Xun ; Guo, Zijian ; Jia, Xin ; Che, Runmin ; Sun, Yiming ; Zhu, Yanfei ; Wang, Ping ; Gong, Xiaoqing ; Ma, Fengwang</creatorcontrib><description>High temperature is an abiotic stress factor that threatens plant growth and development. Autophagy in response to heat stress involves the selective removal of heat-induced protein complexes. Previously, we showed that a crucial autophagy protein from apple, MdATG18a, has a positive effect on drought tolerance. In the present study, we treated transgenic apple (
Malus domestica
) plants overexpressing
MdATG18a
with high temperature and found that autophagy protected them from heat stress. Overexpression of
MdATG18a
in apple enhanced antioxidase activity and contributed to the production of increased beneficial antioxidants under heat stress. Transgenic apple plants exhibited higher photosynthetic capacity, as shown by the rate of CO
2
assimilation, the maximum photochemical efficiency of photosystem II (PSII), the effective quantum yield, and the electron transport rates in photosystems I and II (PSI and PSII, respectively). We also detected elevated autophagic activity and reduced damage to chloroplasts in transgenic plants compared to WT plants. In addition, the transcriptional activities of several
HSP
genes were increased in transgenic apple plants. In summary, we propose that autophagy plays a critical role in basal thermotolerance in apple, primarily through a combination of enhanced antioxidant activity and reduced chloroplast damage.</description><identifier>ISSN: 2662-6810</identifier><identifier>EISSN: 2052-7276</identifier><identifier>DOI: 10.1038/s41438-020-0243-2</identifier><identifier>PMID: 32140230</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/449/1734 ; 631/449/2661/2663 ; Abiotic factors ; Agriculture ; Antioxidants ; Apples ; Autophagy ; Biomedical and Life Sciences ; Carbon dioxide ; Chloroplasts ; Damage ; Drought resistance ; Ecology ; Electron transport ; Heat ; Heat stress ; Heat tolerance ; High temperature ; Life Sciences ; Malus domestica ; Phagocytosis ; Photochemicals ; Photosynthesis ; Photosystem II ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant growth ; Plant protection ; Plant Sciences ; Proteins ; Temperature tolerance ; Transcription ; Transgenic plants</subject><ispartof>Horticulture research, 2020, Vol.7 (1), p.21, Article 21</ispartof><rights>The Author(s) 2020</rights><rights>The Author(s) 2020.</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-247413971dc040722ab5ff700053e7a3bc8b9b13ceab67e5db9b9eb1d51d20d23</citedby><cites>FETCH-LOGICAL-c502t-247413971dc040722ab5ff700053e7a3bc8b9b13ceab67e5db9b9eb1d51d20d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049305/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7049305/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,27924,27925,41120,42189,51576,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32140230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huo, Liuqing</creatorcontrib><creatorcontrib>Sun, Xun</creatorcontrib><creatorcontrib>Guo, Zijian</creatorcontrib><creatorcontrib>Jia, Xin</creatorcontrib><creatorcontrib>Che, Runmin</creatorcontrib><creatorcontrib>Sun, Yiming</creatorcontrib><creatorcontrib>Zhu, Yanfei</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Gong, Xiaoqing</creatorcontrib><creatorcontrib>Ma, Fengwang</creatorcontrib><title>MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts</title><title>Horticulture research</title><addtitle>Hortic Res</addtitle><addtitle>Hortic Res</addtitle><description>High temperature is an abiotic stress factor that threatens plant growth and development. Autophagy in response to heat stress involves the selective removal of heat-induced protein complexes. Previously, we showed that a crucial autophagy protein from apple, MdATG18a, has a positive effect on drought tolerance. In the present study, we treated transgenic apple (
Malus domestica
) plants overexpressing
MdATG18a
with high temperature and found that autophagy protected them from heat stress. Overexpression of
MdATG18a
in apple enhanced antioxidase activity and contributed to the production of increased beneficial antioxidants under heat stress. Transgenic apple plants exhibited higher photosynthetic capacity, as shown by the rate of CO
2
assimilation, the maximum photochemical efficiency of photosystem II (PSII), the effective quantum yield, and the electron transport rates in photosystems I and II (PSI and PSII, respectively). We also detected elevated autophagic activity and reduced damage to chloroplasts in transgenic plants compared to WT plants. In addition, the transcriptional activities of several
HSP
genes were increased in transgenic apple plants. In summary, we propose that autophagy plays a critical role in basal thermotolerance in apple, primarily through a combination of enhanced antioxidant activity and reduced chloroplast damage.</description><subject>631/449/1734</subject><subject>631/449/2661/2663</subject><subject>Abiotic factors</subject><subject>Agriculture</subject><subject>Antioxidants</subject><subject>Apples</subject><subject>Autophagy</subject><subject>Biomedical and Life Sciences</subject><subject>Carbon dioxide</subject><subject>Chloroplasts</subject><subject>Damage</subject><subject>Drought resistance</subject><subject>Ecology</subject><subject>Electron transport</subject><subject>Heat</subject><subject>Heat stress</subject><subject>Heat tolerance</subject><subject>High temperature</subject><subject>Life Sciences</subject><subject>Malus domestica</subject><subject>Phagocytosis</subject><subject>Photochemicals</subject><subject>Photosynthesis</subject><subject>Photosystem II</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant growth</subject><subject>Plant protection</subject><subject>Plant Sciences</subject><subject>Proteins</subject><subject>Temperature tolerance</subject><subject>Transcription</subject><subject>Transgenic plants</subject><issn>2662-6810</issn><issn>2052-7276</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1UU1v1DAQtRCorZb-gF6QJc4p9tiOkwtSVUGLVMSlnC1_zGZTJXawsxX993i1pcCBgzXjmef3nvUIueDskjPRfSiSS9E1DFg9UjTwipwBU9Bo0O3r2rctNG3H2Sk5L-WBMcaVBKH0CTkVwCUDwc5I-hqu7m94Z2l6xIw_l4yljCnScV5yHRXqbLETXXeY57SmCbONHukY6Vq7MmAcPbXLMiF1TzSgz2jLGAca7GwHpGuifjelnJbJlrW8JW-2dip4_lw35PvnT_fXt83dt5sv11d3jVcM1gakllz0mgfPJNMA1qntVtdPKIHaCuc71zsuPFrXalSh3np0PCgegAUQG_LxyLvs3YzBY6x2J7Pkcbb5ySQ7mn83cdyZIT0azWQvqsqGvH8myOnHHstqHtI-x-rZgGg71Yq-lRXFjyifUykZty8KnJlDTOYYk6kxmUNM5mDt3d_WXl78DqUC4AgodRUHzH-k_8_6C52Nn80</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Huo, Liuqing</creator><creator>Sun, Xun</creator><creator>Guo, Zijian</creator><creator>Jia, Xin</creator><creator>Che, Runmin</creator><creator>Sun, Yiming</creator><creator>Zhu, Yanfei</creator><creator>Wang, Ping</creator><creator>Gong, Xiaoqing</creator><creator>Ma, Fengwang</creator><general>Nature Publishing Group UK</general><general>Oxford University Press</general><scope>C6C</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>5PM</scope></search><sort><creationdate>2020</creationdate><title>MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts</title><author>Huo, Liuqing ; 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Autophagy in response to heat stress involves the selective removal of heat-induced protein complexes. Previously, we showed that a crucial autophagy protein from apple, MdATG18a, has a positive effect on drought tolerance. In the present study, we treated transgenic apple (
Malus domestica
) plants overexpressing
MdATG18a
with high temperature and found that autophagy protected them from heat stress. Overexpression of
MdATG18a
in apple enhanced antioxidase activity and contributed to the production of increased beneficial antioxidants under heat stress. Transgenic apple plants exhibited higher photosynthetic capacity, as shown by the rate of CO
2
assimilation, the maximum photochemical efficiency of photosystem II (PSII), the effective quantum yield, and the electron transport rates in photosystems I and II (PSI and PSII, respectively). We also detected elevated autophagic activity and reduced damage to chloroplasts in transgenic plants compared to WT plants. In addition, the transcriptional activities of several
HSP
genes were increased in transgenic apple plants. In summary, we propose that autophagy plays a critical role in basal thermotolerance in apple, primarily through a combination of enhanced antioxidant activity and reduced chloroplast damage.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>32140230</pmid><doi>10.1038/s41438-020-0243-2</doi><oa>free_for_read</oa></addata></record> |
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| subjects | 631/449/1734 631/449/2661/2663 Abiotic factors Agriculture Antioxidants Apples Autophagy Biomedical and Life Sciences Carbon dioxide Chloroplasts Damage Drought resistance Ecology Electron transport Heat Heat stress Heat tolerance High temperature Life Sciences Malus domestica Phagocytosis Photochemicals Photosynthesis Photosystem II Plant Breeding/Biotechnology Plant Genetics and Genomics Plant growth Plant protection Plant Sciences Proteins Temperature tolerance Transcription Transgenic plants |
| title | MdATG18a overexpression improves basal thermotolerance in transgenic apple by decreasing damage to chloroplasts |
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