Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine
Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O 1 BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM R -7Zf(−). An ∼8.2 kb PCR product was ampl...
Gespeichert in:
| Veröffentlicht in: | Journal of biosciences 2009-03, Vol.34 (1), p.45-58 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 58 |
|---|---|
| container_issue | 1 |
| container_start_page | 45 |
| container_title | Journal of biosciences |
| container_volume | 34 |
| creator | Hema, M. Chandran, D. Nagendrakumar, S. B. Madhanmohan, M. Srinivasan, V. A. |
| description | Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O
1
BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM
R
-7Zf(−). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by
in vitro
transcription. Transfection of BHK-21 cells with the
in vitro
transcripts resulted in the production of infectious recombinant FMDV particles as evidenced by cytopathic effects (CPE). Further, characterization of the recombinant virus by immunofluorescence, microneutralization test (MNT), antigen ELISA, RT-PCR, plaque assay and electron microscopy revealed similarity to the parental strain. The immunogenicity of an oil-adjuvant vaccine prepared using the inactivated recombinant virus was tested in guinea pigs and cattle. Neutralizing antibodies were produced in both vaccinated guinea pigs and cattle. Vaccinated animals were protected on challenge. The results demonstrated that the recombinant virus was as stable and effective as the parental strain for the preparation of inactivated vaccine, suggesting the potential application of this strategy to make genetically engineered FMDV vaccines. |
| doi_str_mv | 10.1007/s12038-009-0008-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_236224847</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1894935161</sourcerecordid><originalsourceid>FETCH-LOGICAL-c424t-c7398d74e5222f7076b0ad4d3537a96e1b264899d237dc010f0821f742fdfa5d3</originalsourceid><addsrcrecordid>eNp1kMlKBTEQRRtRcPwAd8F9tDJ0p7PU5wiiC3UdYgaNaNImacF_8KPN4ymuXBRVRZ17C27X7RM4JADiqBAKbMQAshWMmK91WyAFw4Kwcb3NtAfcS0k2u-1SXgCI5Ay2uq9FiqXm2dSQIkoe6YhC9G65zwWZ05tjZF5TdMubT6liHS1-S3N9RjYUp4tDHyE3tH5ODt2Sk_M7RMYBmpFFoRY0NyJEVJ8dmrKbdNa_r0xDgtW1OWhjQnS73YbXr8Xt_fSd7uH87H5xia9vL64Wx9fYcMorNoLJ0QruekqpFyCGR9CWW9YzoeXgyCMd-CilpUxYAwQ8jJR4wam3XveW7XQHK98pp_fZlape0pxje6koGyjlIxcNIivI5FRKdl5NObzp_KkIqGXmapW5apmrZeaKNw1daUpj45PLf8b_i74BePKDrg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>236224847</pqid></control><display><type>article</type><title>Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine</title><source>Indian Academy of Sciences</source><source>Springer Nature - Complete Springer Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Hema, M. ; Chandran, D. ; Nagendrakumar, S. B. ; Madhanmohan, M. ; Srinivasan, V. A.</creator><creatorcontrib>Hema, M. ; Chandran, D. ; Nagendrakumar, S. B. ; Madhanmohan, M. ; Srinivasan, V. A.</creatorcontrib><description>Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O
1
BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM
R
-7Zf(−). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by
in vitro
transcription. Transfection of BHK-21 cells with the
in vitro
transcripts resulted in the production of infectious recombinant FMDV particles as evidenced by cytopathic effects (CPE). Further, characterization of the recombinant virus by immunofluorescence, microneutralization test (MNT), antigen ELISA, RT-PCR, plaque assay and electron microscopy revealed similarity to the parental strain. The immunogenicity of an oil-adjuvant vaccine prepared using the inactivated recombinant virus was tested in guinea pigs and cattle. Neutralizing antibodies were produced in both vaccinated guinea pigs and cattle. Vaccinated animals were protected on challenge. The results demonstrated that the recombinant virus was as stable and effective as the parental strain for the preparation of inactivated vaccine, suggesting the potential application of this strategy to make genetically engineered FMDV vaccines.</description><identifier>ISSN: 0250-5991</identifier><identifier>EISSN: 0973-7138</identifier><identifier>DOI: 10.1007/s12038-009-0008-4</identifier><language>eng</language><publisher>New Delhi: Springer India</publisher><subject>Biomedical and Life Sciences ; Biomedicine ; Cattle ; Cell Biology ; Deoxyribonucleic acid ; DNA ; Foot & mouth disease ; Genetic engineering ; Immunogenicity ; Life Sciences ; Microbiology ; Molecular biology ; Plant Sciences ; Vaccines ; Viruses ; Zoology</subject><ispartof>Journal of biosciences, 2009-03, Vol.34 (1), p.45-58</ispartof><rights>Indian Academy of Sciences 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c424t-c7398d74e5222f7076b0ad4d3537a96e1b264899d237dc010f0821f742fdfa5d3</citedby><cites>FETCH-LOGICAL-c424t-c7398d74e5222f7076b0ad4d3537a96e1b264899d237dc010f0821f742fdfa5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12038-009-0008-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12038-009-0008-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Hema, M.</creatorcontrib><creatorcontrib>Chandran, D.</creatorcontrib><creatorcontrib>Nagendrakumar, S. B.</creatorcontrib><creatorcontrib>Madhanmohan, M.</creatorcontrib><creatorcontrib>Srinivasan, V. A.</creatorcontrib><title>Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine</title><title>Journal of biosciences</title><addtitle>J Biosci</addtitle><description>Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O
1
BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM
R
-7Zf(−). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by
in vitro
transcription. Transfection of BHK-21 cells with the
in vitro
transcripts resulted in the production of infectious recombinant FMDV particles as evidenced by cytopathic effects (CPE). Further, characterization of the recombinant virus by immunofluorescence, microneutralization test (MNT), antigen ELISA, RT-PCR, plaque assay and electron microscopy revealed similarity to the parental strain. The immunogenicity of an oil-adjuvant vaccine prepared using the inactivated recombinant virus was tested in guinea pigs and cattle. Neutralizing antibodies were produced in both vaccinated guinea pigs and cattle. Vaccinated animals were protected on challenge. The results demonstrated that the recombinant virus was as stable and effective as the parental strain for the preparation of inactivated vaccine, suggesting the potential application of this strategy to make genetically engineered FMDV vaccines.</description><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Cattle</subject><subject>Cell Biology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Foot & mouth disease</subject><subject>Genetic engineering</subject><subject>Immunogenicity</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Molecular biology</subject><subject>Plant Sciences</subject><subject>Vaccines</subject><subject>Viruses</subject><subject>Zoology</subject><issn>0250-5991</issn><issn>0973-7138</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kMlKBTEQRRtRcPwAd8F9tDJ0p7PU5wiiC3UdYgaNaNImacF_8KPN4ymuXBRVRZ17C27X7RM4JADiqBAKbMQAshWMmK91WyAFw4Kwcb3NtAfcS0k2u-1SXgCI5Ay2uq9FiqXm2dSQIkoe6YhC9G65zwWZ05tjZF5TdMubT6liHS1-S3N9RjYUp4tDHyE3tH5ODt2Sk_M7RMYBmpFFoRY0NyJEVJ8dmrKbdNa_r0xDgtW1OWhjQnS73YbXr8Xt_fSd7uH87H5xia9vL64Wx9fYcMorNoLJ0QruekqpFyCGR9CWW9YzoeXgyCMd-CilpUxYAwQ8jJR4wam3XveW7XQHK98pp_fZlape0pxje6koGyjlIxcNIivI5FRKdl5NObzp_KkIqGXmapW5apmrZeaKNw1daUpj45PLf8b_i74BePKDrg</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Hema, M.</creator><creator>Chandran, D.</creator><creator>Nagendrakumar, S. B.</creator><creator>Madhanmohan, M.</creator><creator>Srinivasan, V. A.</creator><general>Springer India</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.F</scope><scope>L.G</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope></search><sort><creationdate>20090301</creationdate><title>Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine</title><author>Hema, M. ; Chandran, D. ; Nagendrakumar, S. B. ; Madhanmohan, M. ; Srinivasan, V. A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c424t-c7398d74e5222f7076b0ad4d3537a96e1b264899d237dc010f0821f742fdfa5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Cattle</topic><topic>Cell Biology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Foot & mouth disease</topic><topic>Genetic engineering</topic><topic>Immunogenicity</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Molecular biology</topic><topic>Plant Sciences</topic><topic>Vaccines</topic><topic>Viruses</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hema, M.</creatorcontrib><creatorcontrib>Chandran, D.</creatorcontrib><creatorcontrib>Nagendrakumar, S. B.</creatorcontrib><creatorcontrib>Madhanmohan, M.</creatorcontrib><creatorcontrib>Srinivasan, V. A.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><jtitle>Journal of biosciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hema, M.</au><au>Chandran, D.</au><au>Nagendrakumar, S. B.</au><au>Madhanmohan, M.</au><au>Srinivasan, V. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine</atitle><jtitle>Journal of biosciences</jtitle><stitle>J Biosci</stitle><date>2009-03-01</date><risdate>2009</risdate><volume>34</volume><issue>1</issue><spage>45</spage><epage>58</epage><pages>45-58</pages><issn>0250-5991</issn><eissn>0973-7138</eissn><abstract>Foot-and-mouth disease virus (FMDV) serotype O is the most predominant among the endemic serotypes in India. A stable, full-length cDNA clone of FMDV type O
1
BFS 1860 preceded by a bacteriophage T7 polymerase promoter was assembled in a plasmid vector pGEM
R
-7Zf(−). An ∼8.2 kb PCR product was amplified from the cDNA clone and a full-length RNA was generated from it by
in vitro
transcription. Transfection of BHK-21 cells with the
in vitro
transcripts resulted in the production of infectious recombinant FMDV particles as evidenced by cytopathic effects (CPE). Further, characterization of the recombinant virus by immunofluorescence, microneutralization test (MNT), antigen ELISA, RT-PCR, plaque assay and electron microscopy revealed similarity to the parental strain. The immunogenicity of an oil-adjuvant vaccine prepared using the inactivated recombinant virus was tested in guinea pigs and cattle. Neutralizing antibodies were produced in both vaccinated guinea pigs and cattle. Vaccinated animals were protected on challenge. The results demonstrated that the recombinant virus was as stable and effective as the parental strain for the preparation of inactivated vaccine, suggesting the potential application of this strategy to make genetically engineered FMDV vaccines.</abstract><cop>New Delhi</cop><pub>Springer India</pub><doi>10.1007/s12038-009-0008-4</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0250-5991 |
| ispartof | Journal of biosciences, 2009-03, Vol.34 (1), p.45-58 |
| issn | 0250-5991 0973-7138 |
| language | eng |
| recordid | cdi_proquest_journals_236224847 |
| source | Indian Academy of Sciences; Springer Nature - Complete Springer Journals; EZB-FREE-00999 freely available EZB journals |
| subjects | Biomedical and Life Sciences Biomedicine Cattle Cell Biology Deoxyribonucleic acid DNA Foot & mouth disease Genetic engineering Immunogenicity Life Sciences Microbiology Molecular biology Plant Sciences Vaccines Viruses Zoology |
| title | Construction of an infectious cDNA clone of foot-and-mouth disease virus type O1BFS 1860 and its use in the preparation of candidate vaccine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T18%3A07%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Construction%20of%20an%20infectious%20cDNA%20clone%20of%20foot-and-mouth%20disease%20virus%20type%20O1BFS%201860%20and%20its%20use%20in%20the%20preparation%20of%20candidate%20vaccine&rft.jtitle=Journal%20of%20biosciences&rft.au=Hema,%20M.&rft.date=2009-03-01&rft.volume=34&rft.issue=1&rft.spage=45&rft.epage=58&rft.pages=45-58&rft.issn=0250-5991&rft.eissn=0973-7138&rft_id=info:doi/10.1007/s12038-009-0008-4&rft_dat=%3Cproquest_cross%3E1894935161%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=236224847&rft_id=info:pmid/&rfr_iscdi=true |