Chloroplast movements in the field
An ecophysiological understanding of chloroplast movements in leaves requires measurement of these movements under field conditions. A field-portable instrument was constructed, based on a pulsed measuring beam and lock-in detection that measures chloroplast movements in attached leaves by sensing t...
Gespeichert in:
Veröffentlicht in: | Plant, cell and environment cell and environment, 2003-12, Vol.26 (12), p.2005-2014 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 2014 |
---|---|
container_issue | 12 |
container_start_page | 2005 |
container_title | Plant, cell and environment |
container_volume | 26 |
creator | Williams, W.E Gorton, H.L Witiak, S.M |
description | An ecophysiological understanding of chloroplast movements in leaves requires measurement of these movements under field conditions. A field-portable instrument was constructed, based on a pulsed measuring beam and lock-in detection that measures chloroplast movements in attached leaves by sensing the resultant changes in leaf transmittance. In the instrument and generally in nature, leaves are illuminated obliquely, in contrast with the perpendicular illumination used in most laboratory experiments on chloroplast movement. Microscopic analysis of cells illuminated obliquely with bright light verified that chloroplasts move out of the light path, and transmittance changes in response to oblique light were robust. Chloroplast movements in Alocasia brisbanensis under natural sunlight express kinetics and light requirements expected from laboratory observations: chloroplasts were in the periclinal position at dawn and dusk, anticlinal position in full sunlight midday, and in an intermediate position at night. Movement in response to bright light was rapid allowing responses to brief sunflecks. Movements in Helianthus tuberosum, Eustrephus latifolius and Cissus hypoglauca were qualitatively similar with differing kinetics and magnitude. In all four species, chloroplasts were in motion most of the time, rarely achieving the extreme anticlinal or periclinal positions. |
doi_str_mv | 10.1046/j.0016-8025.2003.01117.x |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_236020835</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>527322931</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3927-76305886721932a7ef86fe6e27fafdf8ad1d010e6dfd065411df8d6779ec670c3</originalsourceid><addsrcrecordid>eNqNkF1LwzAUhoMoOKe_wTLwsvWcpE3SCy-kzA8YKOiuQ2gT19K1M-l0-_embuCtVycc3uc94SEkQkgQUn7bJADIYwk0SygASwARRbI7IRNkPIsZpHBKJoApxELkeE4uvG8gQKnIJ2RWrNre9ZtW-yFa919mbbrBR3UXDSsT2dq01SU5s7r15uo4p2T5MH8vnuLFy-Nzcb-IS5ZTEQvOIJOSC4o5o1oYK7k13FBhta2s1BVWgGB4ZSvgWYoYlhUPfzIlF1CyKZkdejeu_9waP6im37ounFSUcaAgWRZC8hAqXe-9M1ZtXL3Wbq8Q1ChENWoUokYhahSifoWoXUBvjv3al7q1Tndl7f_4jDEpWRpyd4fcd92a_b_71WsxH1-Bvz7wVvdKf7hwY_lGARlAHuozyn4Aglx6Uw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>236020835</pqid></control><display><type>article</type><title>Chloroplast movements in the field</title><source>Wiley Online Library Journals</source><source>Wiley Journals</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Williams, W.E ; Gorton, H.L ; Witiak, S.M</creator><creatorcontrib>Williams, W.E ; Gorton, H.L ; Witiak, S.M</creatorcontrib><description>An ecophysiological understanding of chloroplast movements in leaves requires measurement of these movements under field conditions. A field-portable instrument was constructed, based on a pulsed measuring beam and lock-in detection that measures chloroplast movements in attached leaves by sensing the resultant changes in leaf transmittance. In the instrument and generally in nature, leaves are illuminated obliquely, in contrast with the perpendicular illumination used in most laboratory experiments on chloroplast movement. Microscopic analysis of cells illuminated obliquely with bright light verified that chloroplasts move out of the light path, and transmittance changes in response to oblique light were robust. Chloroplast movements in Alocasia brisbanensis under natural sunlight express kinetics and light requirements expected from laboratory observations: chloroplasts were in the periclinal position at dawn and dusk, anticlinal position in full sunlight midday, and in an intermediate position at night. Movement in response to bright light was rapid allowing responses to brief sunflecks. Movements in Helianthus tuberosum, Eustrephus latifolius and Cissus hypoglauca were qualitatively similar with differing kinetics and magnitude. In all four species, chloroplasts were in motion most of the time, rarely achieving the extreme anticlinal or periclinal positions.</description><identifier>ISSN: 0140-7791</identifier><identifier>EISSN: 1365-3040</identifier><identifier>DOI: 10.1046/j.0016-8025.2003.01117.x</identifier><identifier>CODEN: PLCEDV</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Alocasia ; Alocasia brisbanensis ; Animal and plant ecology ; Animal, plant and microbial ecology ; anticlinal ; Autoecology ; Biological and medical sciences ; Cell motion ; Cell physiology ; chloroplast movement ; chloroplasts ; Cissus ; Cissus hypoglauca ; Eustrephus latifolius ; Fundamental and applied biological sciences. Psychology ; Helianthus ; Helianthus tuberosum ; leaf optics ; light intensity ; lighting ; periclinal ; Plant physiology and development ; Plants and fungi ; solar radiation ; sunflecks</subject><ispartof>Plant, cell and environment, 2003-12, Vol.26 (12), p.2005-2014</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. Dec 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3927-76305886721932a7ef86fe6e27fafdf8ad1d010e6dfd065411df8d6779ec670c3</citedby><cites>FETCH-LOGICAL-c3927-76305886721932a7ef86fe6e27fafdf8ad1d010e6dfd065411df8d6779ec670c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.0016-8025.2003.01117.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.0016-8025.2003.01117.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15338834$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Williams, W.E</creatorcontrib><creatorcontrib>Gorton, H.L</creatorcontrib><creatorcontrib>Witiak, S.M</creatorcontrib><title>Chloroplast movements in the field</title><title>Plant, cell and environment</title><description>An ecophysiological understanding of chloroplast movements in leaves requires measurement of these movements under field conditions. A field-portable instrument was constructed, based on a pulsed measuring beam and lock-in detection that measures chloroplast movements in attached leaves by sensing the resultant changes in leaf transmittance. In the instrument and generally in nature, leaves are illuminated obliquely, in contrast with the perpendicular illumination used in most laboratory experiments on chloroplast movement. Microscopic analysis of cells illuminated obliquely with bright light verified that chloroplasts move out of the light path, and transmittance changes in response to oblique light were robust. Chloroplast movements in Alocasia brisbanensis under natural sunlight express kinetics and light requirements expected from laboratory observations: chloroplasts were in the periclinal position at dawn and dusk, anticlinal position in full sunlight midday, and in an intermediate position at night. Movement in response to bright light was rapid allowing responses to brief sunflecks. Movements in Helianthus tuberosum, Eustrephus latifolius and Cissus hypoglauca were qualitatively similar with differing kinetics and magnitude. In all four species, chloroplasts were in motion most of the time, rarely achieving the extreme anticlinal or periclinal positions.</description><subject>Alocasia</subject><subject>Alocasia brisbanensis</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>anticlinal</subject><subject>Autoecology</subject><subject>Biological and medical sciences</subject><subject>Cell motion</subject><subject>Cell physiology</subject><subject>chloroplast movement</subject><subject>chloroplasts</subject><subject>Cissus</subject><subject>Cissus hypoglauca</subject><subject>Eustrephus latifolius</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Helianthus</subject><subject>Helianthus tuberosum</subject><subject>leaf optics</subject><subject>light intensity</subject><subject>lighting</subject><subject>periclinal</subject><subject>Plant physiology and development</subject><subject>Plants and fungi</subject><subject>solar radiation</subject><subject>sunflecks</subject><issn>0140-7791</issn><issn>1365-3040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqNkF1LwzAUhoMoOKe_wTLwsvWcpE3SCy-kzA8YKOiuQ2gT19K1M-l0-_embuCtVycc3uc94SEkQkgQUn7bJADIYwk0SygASwARRbI7IRNkPIsZpHBKJoApxELkeE4uvG8gQKnIJ2RWrNre9ZtW-yFa919mbbrBR3UXDSsT2dq01SU5s7r15uo4p2T5MH8vnuLFy-Nzcb-IS5ZTEQvOIJOSC4o5o1oYK7k13FBhta2s1BVWgGB4ZSvgWYoYlhUPfzIlF1CyKZkdejeu_9waP6im37ounFSUcaAgWRZC8hAqXe-9M1ZtXL3Wbq8Q1ChENWoUokYhahSifoWoXUBvjv3al7q1Tndl7f_4jDEpWRpyd4fcd92a_b_71WsxH1-Bvz7wVvdKf7hwY_lGARlAHuozyn4Aglx6Uw</recordid><startdate>200312</startdate><enddate>200312</enddate><creator>Williams, W.E</creator><creator>Gorton, H.L</creator><creator>Witiak, S.M</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Wiley Subscription Services, Inc</general><scope>FBQ</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>200312</creationdate><title>Chloroplast movements in the field</title><author>Williams, W.E ; Gorton, H.L ; Witiak, S.M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3927-76305886721932a7ef86fe6e27fafdf8ad1d010e6dfd065411df8d6779ec670c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Alocasia</topic><topic>Alocasia brisbanensis</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>anticlinal</topic><topic>Autoecology</topic><topic>Biological and medical sciences</topic><topic>Cell motion</topic><topic>Cell physiology</topic><topic>chloroplast movement</topic><topic>chloroplasts</topic><topic>Cissus</topic><topic>Cissus hypoglauca</topic><topic>Eustrephus latifolius</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Helianthus</topic><topic>Helianthus tuberosum</topic><topic>leaf optics</topic><topic>light intensity</topic><topic>lighting</topic><topic>periclinal</topic><topic>Plant physiology and development</topic><topic>Plants and fungi</topic><topic>solar radiation</topic><topic>sunflecks</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Williams, W.E</creatorcontrib><creatorcontrib>Gorton, H.L</creatorcontrib><creatorcontrib>Witiak, S.M</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Plant, cell and environment</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Williams, W.E</au><au>Gorton, H.L</au><au>Witiak, S.M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chloroplast movements in the field</atitle><jtitle>Plant, cell and environment</jtitle><date>2003-12</date><risdate>2003</risdate><volume>26</volume><issue>12</issue><spage>2005</spage><epage>2014</epage><pages>2005-2014</pages><issn>0140-7791</issn><eissn>1365-3040</eissn><coden>PLCEDV</coden><abstract>An ecophysiological understanding of chloroplast movements in leaves requires measurement of these movements under field conditions. A field-portable instrument was constructed, based on a pulsed measuring beam and lock-in detection that measures chloroplast movements in attached leaves by sensing the resultant changes in leaf transmittance. In the instrument and generally in nature, leaves are illuminated obliquely, in contrast with the perpendicular illumination used in most laboratory experiments on chloroplast movement. Microscopic analysis of cells illuminated obliquely with bright light verified that chloroplasts move out of the light path, and transmittance changes in response to oblique light were robust. Chloroplast movements in Alocasia brisbanensis under natural sunlight express kinetics and light requirements expected from laboratory observations: chloroplasts were in the periclinal position at dawn and dusk, anticlinal position in full sunlight midday, and in an intermediate position at night. Movement in response to bright light was rapid allowing responses to brief sunflecks. Movements in Helianthus tuberosum, Eustrephus latifolius and Cissus hypoglauca were qualitatively similar with differing kinetics and magnitude. In all four species, chloroplasts were in motion most of the time, rarely achieving the extreme anticlinal or periclinal positions.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><doi>10.1046/j.0016-8025.2003.01117.x</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0140-7791 |
ispartof | Plant, cell and environment, 2003-12, Vol.26 (12), p.2005-2014 |
issn | 0140-7791 1365-3040 |
language | eng |
recordid | cdi_proquest_journals_236020835 |
source | Wiley Online Library Journals; Wiley Journals; Alma/SFX Local Collection; EZB Electronic Journals Library |
subjects | Alocasia Alocasia brisbanensis Animal and plant ecology Animal, plant and microbial ecology anticlinal Autoecology Biological and medical sciences Cell motion Cell physiology chloroplast movement chloroplasts Cissus Cissus hypoglauca Eustrephus latifolius Fundamental and applied biological sciences. Psychology Helianthus Helianthus tuberosum leaf optics light intensity lighting periclinal Plant physiology and development Plants and fungi solar radiation sunflecks |
title | Chloroplast movements in the field |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T17%3A29%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Chloroplast%20movements%20in%20the%20field&rft.jtitle=Plant,%20cell%20and%20environment&rft.au=Williams,%20W.E&rft.date=2003-12&rft.volume=26&rft.issue=12&rft.spage=2005&rft.epage=2014&rft.pages=2005-2014&rft.issn=0140-7791&rft.eissn=1365-3040&rft.coden=PLCEDV&rft_id=info:doi/10.1046/j.0016-8025.2003.01117.x&rft_dat=%3Cproquest_cross%3E527322931%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=236020835&rft_id=info:pmid/&rfr_iscdi=true |