Exogenous polyamines enhance somatic embryogenesis and "Agrobacterium tumefaciens"-mediated transformation efficiency in sugarcane ("Saccharum" spp. hybrid)
The influence of exogenous polyamines (PAs) on somatic embryogenesis from immature leaf roll expiants and Agrobacterium tumefaciens-mediated transformation of embryogenie callus of Saccharum spp. (sugarcane) 'Co 86032' was examined. Immature leaf roll-derived embryogenie callus was obtaine...
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| Veröffentlicht in: | In vitro cellular & developmental biology. Plant 2020-02, Vol.56 (1), p.29-40 |
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| creator | Sathish, Dorairaj Theboral, Jeevaraj Vasudevan, Venkatachalam Pavan, Gadamchetty Ajithan, Chandrasekaran Appunu, Chinnaswamy Manickavasagam, Markandan |
| description | The influence of exogenous polyamines (PAs) on somatic embryogenesis from immature leaf roll expiants and Agrobacterium tumefaciens-mediated transformation of embryogenie callus of Saccharum spp. (sugarcane) 'Co 86032' was examined. Immature leaf roll-derived embryogenie callus was obtained on Murashige and Skoog with Gamborg B5 vitamins (MSB₅) medium containing 3 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). Various concentrations of PAs along with 2 mg L⁻¹ 2,4-D and 0.5 mg L⁻¹ kinetin (Kin) were tested for somatic embryo induction. A total of 106 somatic embryos per 250 mg of callus (96.3% responsive expiants) were obtained on medium supplemented with 20 mg L⁻¹ putrescine (PUT) and 92.0% of the somatic embryos matured and produced 98 shoots per 250 mg of callus. Somatic embryo induction and maturation was increased more than two- and threefold, respectively, on PUT-supplemented medium compared to control cultures. Histomorphological analyses of various developmental stages verified somatic embryogenesis from immature leaf roll expiants. The rooted plantlets were successfully hardened and exhibited normal growth. The efficiency of A. tumefaciens-mediated transformation of embryogenie callus using various concentrations of PAs in the infection, co-cultivation, and regeneration media was also assessed. Putrescine at 20 mg L⁻¹ showed the highest regeneration (54.4%) and transformation (35.8%) efficiencies, which were more than twofold higher than the control treatment. These results demonstrate that exogenously supplied PAs improve plant regeneration using somatic embryogenesis and A. tumefaciens-mediatod transformation of embryogénie callus of sugarcane 'Co 86032'. |
| doi_str_mv | 10.1007/s11627-019-10022-6 |
| format | Article |
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Immature leaf roll-derived embryogenie callus was obtained on Murashige and Skoog with Gamborg B5 vitamins (MSB₅) medium containing 3 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). Various concentrations of PAs along with 2 mg L⁻¹ 2,4-D and 0.5 mg L⁻¹ kinetin (Kin) were tested for somatic embryo induction. A total of 106 somatic embryos per 250 mg of callus (96.3% responsive expiants) were obtained on medium supplemented with 20 mg L⁻¹ putrescine (PUT) and 92.0% of the somatic embryos matured and produced 98 shoots per 250 mg of callus. Somatic embryo induction and maturation was increased more than two- and threefold, respectively, on PUT-supplemented medium compared to control cultures. Histomorphological analyses of various developmental stages verified somatic embryogenesis from immature leaf roll expiants. The rooted plantlets were successfully hardened and exhibited normal growth. The efficiency of A. tumefaciens-mediated transformation of embryogenie callus using various concentrations of PAs in the infection, co-cultivation, and regeneration media was also assessed. Putrescine at 20 mg L⁻¹ showed the highest regeneration (54.4%) and transformation (35.8%) efficiencies, which were more than twofold higher than the control treatment. These results demonstrate that exogenously supplied PAs improve plant regeneration using somatic embryogenesis and A. tumefaciens-mediatod transformation of embryogénie callus of sugarcane 'Co 86032'.</description><identifier>ISSN: 1054-5476</identifier><identifier>EISSN: 1475-2689</identifier><identifier>DOI: 10.1007/s11627-019-10022-6</identifier><language>eng</language><publisher>New York: Springer Science + Business Media, LLC</publisher><subject>2,4-D ; Acids ; Agrobacterium tumefaciens ; Biomedical and Life Sciences ; Callus ; Cell Biology ; Cultivation ; Culture media ; Developmental Biology ; Developmental stages ; Dichlorophenoxyacetic acid ; Efficiency ; EMBRYOGENESIS/SOMATIC EMBRYOGENESIS ; Embryonic growth stage ; Embryos ; Explants ; Flowers & plants ; Fluorescent lighting ; Kinetin ; Leaves ; Life Sciences ; Maturation ; Morphology ; Physiology ; Plant Breeding/Biotechnology ; Plant Genetics and Genomics ; Plant Sciences ; Plantlets ; Polyamines ; Putrescine ; Regeneration ; Saccharum ; Shoots ; Somatic embryogenesis ; Sugarcane ; Transformations ; Vitamins</subject><ispartof>In vitro cellular & developmental biology. 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Plant</title><addtitle>In Vitro Cell.Dev.Biol.-Plant</addtitle><description>The influence of exogenous polyamines (PAs) on somatic embryogenesis from immature leaf roll expiants and Agrobacterium tumefaciens-mediated transformation of embryogenie callus of Saccharum spp. (sugarcane) 'Co 86032' was examined. Immature leaf roll-derived embryogenie callus was obtained on Murashige and Skoog with Gamborg B5 vitamins (MSB₅) medium containing 3 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). Various concentrations of PAs along with 2 mg L⁻¹ 2,4-D and 0.5 mg L⁻¹ kinetin (Kin) were tested for somatic embryo induction. A total of 106 somatic embryos per 250 mg of callus (96.3% responsive expiants) were obtained on medium supplemented with 20 mg L⁻¹ putrescine (PUT) and 92.0% of the somatic embryos matured and produced 98 shoots per 250 mg of callus. Somatic embryo induction and maturation was increased more than two- and threefold, respectively, on PUT-supplemented medium compared to control cultures. Histomorphological analyses of various developmental stages verified somatic embryogenesis from immature leaf roll expiants. The rooted plantlets were successfully hardened and exhibited normal growth. The efficiency of A. tumefaciens-mediated transformation of embryogenie callus using various concentrations of PAs in the infection, co-cultivation, and regeneration media was also assessed. Putrescine at 20 mg L⁻¹ showed the highest regeneration (54.4%) and transformation (35.8%) efficiencies, which were more than twofold higher than the control treatment. These results demonstrate that exogenously supplied PAs improve plant regeneration using somatic embryogenesis and A. tumefaciens-mediatod transformation of embryogénie callus of sugarcane 'Co 86032'.</description><subject>2,4-D</subject><subject>Acids</subject><subject>Agrobacterium tumefaciens</subject><subject>Biomedical and Life Sciences</subject><subject>Callus</subject><subject>Cell Biology</subject><subject>Cultivation</subject><subject>Culture media</subject><subject>Developmental Biology</subject><subject>Developmental stages</subject><subject>Dichlorophenoxyacetic acid</subject><subject>Efficiency</subject><subject>EMBRYOGENESIS/SOMATIC EMBRYOGENESIS</subject><subject>Embryonic growth stage</subject><subject>Embryos</subject><subject>Explants</subject><subject>Flowers & plants</subject><subject>Fluorescent lighting</subject><subject>Kinetin</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Maturation</subject><subject>Morphology</subject><subject>Physiology</subject><subject>Plant Breeding/Biotechnology</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Sciences</subject><subject>Plantlets</subject><subject>Polyamines</subject><subject>Putrescine</subject><subject>Regeneration</subject><subject>Saccharum</subject><subject>Shoots</subject><subject>Somatic embryogenesis</subject><subject>Sugarcane</subject><subject>Transformations</subject><subject>Vitamins</subject><issn>1054-5476</issn><issn>1475-2689</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNp9kc9u1DAQxiMEEqXwApysRUjtwcV_YntzrKqWIlXqAThbs85416vGDnYikXfpw-IlSHDiNDPS7_tmRl_TvOfsijNmPhXOtTCU8Y7WWQiqXzRnvDWKCr3tXtaeqZaq1ujXzZtSjowxzrg5a55vf6Y9xjQXMqanBYYQsRCMB4gOSUkDTMERHHZ5OXFYQiEQe7K53ue0AzdhDvNApnlADy5gLBs6YB9gwp5MGWLxKZ9MUiTofTghbiEhkjLvITuISC42X8G5A-R52JAyjlfksOxy6C_fNq88PBV896eeN9_vbr_d3NOHx89fbq4fqJNtN1HpuOx60bmeSyagvl8n41vOXKu07pxnwHagzVZKLXsBXvWgGXItBTd6K8-bD6vvmNOPGctkj2nOsa60QqpOCsm2qlJipVxOpWT0dsxhgLxYzuwpBbumYGsK9ncKVleRXEWlwnGP-a_1f1UfV9WxTCn_u6eeYmyrRKcZV_IXpViYGA</recordid><startdate>20200201</startdate><enddate>20200201</enddate><creator>Sathish, Dorairaj</creator><creator>Theboral, Jeevaraj</creator><creator>Vasudevan, Venkatachalam</creator><creator>Pavan, Gadamchetty</creator><creator>Ajithan, Chandrasekaran</creator><creator>Appunu, Chinnaswamy</creator><creator>Manickavasagam, Markandan</creator><general>Springer Science + Business Media, LLC</general><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>4T-</scope><scope>4U-</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AF</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>S0X</scope></search><sort><creationdate>20200201</creationdate><title>Exogenous polyamines enhance somatic embryogenesis and "Agrobacterium tumefaciens"-mediated transformation efficiency in sugarcane ("Saccharum" spp. hybrid)</title><author>Sathish, Dorairaj ; Theboral, Jeevaraj ; Vasudevan, Venkatachalam ; Pavan, Gadamchetty ; Ajithan, Chandrasekaran ; Appunu, Chinnaswamy ; Manickavasagam, Markandan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-3c139d29cd1302a0229d27f410c45669cf0a0ba6783363d2af5da60e163217683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>2,4-D</topic><topic>Acids</topic><topic>Agrobacterium tumefaciens</topic><topic>Biomedical and Life Sciences</topic><topic>Callus</topic><topic>Cell Biology</topic><topic>Cultivation</topic><topic>Culture media</topic><topic>Developmental Biology</topic><topic>Developmental stages</topic><topic>Dichlorophenoxyacetic acid</topic><topic>Efficiency</topic><topic>EMBRYOGENESIS/SOMATIC EMBRYOGENESIS</topic><topic>Embryonic growth stage</topic><topic>Embryos</topic><topic>Explants</topic><topic>Flowers & plants</topic><topic>Fluorescent lighting</topic><topic>Kinetin</topic><topic>Leaves</topic><topic>Life Sciences</topic><topic>Maturation</topic><topic>Morphology</topic><topic>Physiology</topic><topic>Plant Breeding/Biotechnology</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Sciences</topic><topic>Plantlets</topic><topic>Polyamines</topic><topic>Putrescine</topic><topic>Regeneration</topic><topic>Saccharum</topic><topic>Shoots</topic><topic>Somatic embryogenesis</topic><topic>Sugarcane</topic><topic>Transformations</topic><topic>Vitamins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sathish, Dorairaj</creatorcontrib><creatorcontrib>Theboral, Jeevaraj</creatorcontrib><creatorcontrib>Vasudevan, Venkatachalam</creatorcontrib><creatorcontrib>Pavan, Gadamchetty</creatorcontrib><creatorcontrib>Ajithan, Chandrasekaran</creatorcontrib><creatorcontrib>Appunu, Chinnaswamy</creatorcontrib><creatorcontrib>Manickavasagam, Markandan</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Docstoc</collection><collection>University Readers</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>SIRS Editorial</collection><jtitle>In vitro cellular & developmental biology. Plant</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sathish, Dorairaj</au><au>Theboral, Jeevaraj</au><au>Vasudevan, Venkatachalam</au><au>Pavan, Gadamchetty</au><au>Ajithan, Chandrasekaran</au><au>Appunu, Chinnaswamy</au><au>Manickavasagam, Markandan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exogenous polyamines enhance somatic embryogenesis and "Agrobacterium tumefaciens"-mediated transformation efficiency in sugarcane ("Saccharum" spp. hybrid)</atitle><jtitle>In vitro cellular & developmental biology. Plant</jtitle><stitle>In Vitro Cell.Dev.Biol.-Plant</stitle><date>2020-02-01</date><risdate>2020</risdate><volume>56</volume><issue>1</issue><spage>29</spage><epage>40</epage><pages>29-40</pages><issn>1054-5476</issn><eissn>1475-2689</eissn><abstract>The influence of exogenous polyamines (PAs) on somatic embryogenesis from immature leaf roll expiants and Agrobacterium tumefaciens-mediated transformation of embryogenie callus of Saccharum spp. (sugarcane) 'Co 86032' was examined. Immature leaf roll-derived embryogenie callus was obtained on Murashige and Skoog with Gamborg B5 vitamins (MSB₅) medium containing 3 mg L⁻¹ 2,4-dichlorophenoxyacetic acid (2,4-D). Various concentrations of PAs along with 2 mg L⁻¹ 2,4-D and 0.5 mg L⁻¹ kinetin (Kin) were tested for somatic embryo induction. A total of 106 somatic embryos per 250 mg of callus (96.3% responsive expiants) were obtained on medium supplemented with 20 mg L⁻¹ putrescine (PUT) and 92.0% of the somatic embryos matured and produced 98 shoots per 250 mg of callus. Somatic embryo induction and maturation was increased more than two- and threefold, respectively, on PUT-supplemented medium compared to control cultures. Histomorphological analyses of various developmental stages verified somatic embryogenesis from immature leaf roll expiants. The rooted plantlets were successfully hardened and exhibited normal growth. The efficiency of A. tumefaciens-mediated transformation of embryogenie callus using various concentrations of PAs in the infection, co-cultivation, and regeneration media was also assessed. Putrescine at 20 mg L⁻¹ showed the highest regeneration (54.4%) and transformation (35.8%) efficiencies, which were more than twofold higher than the control treatment. These results demonstrate that exogenously supplied PAs improve plant regeneration using somatic embryogenesis and A. tumefaciens-mediatod transformation of embryogénie callus of sugarcane 'Co 86032'.</abstract><cop>New York</cop><pub>Springer Science + Business Media, LLC</pub><doi>10.1007/s11627-019-10022-6</doi><tpages>12</tpages></addata></record> |
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| subjects | 2,4-D Acids Agrobacterium tumefaciens Biomedical and Life Sciences Callus Cell Biology Cultivation Culture media Developmental Biology Developmental stages Dichlorophenoxyacetic acid Efficiency EMBRYOGENESIS/SOMATIC EMBRYOGENESIS Embryonic growth stage Embryos Explants Flowers & plants Fluorescent lighting Kinetin Leaves Life Sciences Maturation Morphology Physiology Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Sciences Plantlets Polyamines Putrescine Regeneration Saccharum Shoots Somatic embryogenesis Sugarcane Transformations Vitamins |
| title | Exogenous polyamines enhance somatic embryogenesis and "Agrobacterium tumefaciens"-mediated transformation efficiency in sugarcane ("Saccharum" spp. hybrid) |
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