Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics

Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers....

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Veröffentlicht in:Neurosurgery 2012, Vol.70 (1), p.188-197
Hauptverfasser: BAGRIACIK, Emin Umit, BAYKANER, Mustafa Kemali, YAMAN, Melek, SIVRIKAYA, Gizem, DURDAG, Emre, EMMEZ, Hakan, FINCAN, Gökçe Öztürk, BÖRCEK, Alp Özgün, SECEN, Ahmet Eren, ERCAN, Sevim
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container_end_page 197
container_issue 1
container_start_page 188
container_title Neurosurgery
container_volume 70
creator BAGRIACIK, Emin Umit
BAYKANER, Mustafa Kemali
YAMAN, Melek
SIVRIKAYA, Gizem
DURDAG, Emre
EMMEZ, Hakan
FINCAN, Gökçe Öztürk
BÖRCEK, Alp Özgün
SECEN, Ahmet Eren
ERCAN, Sevim
description Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers. To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs. Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs. We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully. Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.
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Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers. To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs. Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs. We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully. 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subjects Adolescent
Antineoplastic Agents - pharmacology
Astrocytoma - complications
Astrocytoma - pathology
Biological and medical sciences
Brain cancer
Brain Neoplasms - complications
Brain Neoplasms - pathology
Cell Count
Cell culture
Cell Proliferation - drug effects
Drug Screening Assays, Antitumor
Glial Fibrillary Acidic Protein - metabolism
Humans
Magnetic Resonance Imaging
Male
Medical sciences
Neurosurgery
Primary Cell Culture - methods
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tetrazolium Salts
Thiazoles
Thymidine - metabolism
Tritium
Tumor Cells, Cultured - drug effects
Tumor Cells, Cultured - metabolism
title Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics
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