Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics
Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers....
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Veröffentlicht in: | Neurosurgery 2012, Vol.70 (1), p.188-197 |
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creator | BAGRIACIK, Emin Umit BAYKANER, Mustafa Kemali YAMAN, Melek SIVRIKAYA, Gizem DURDAG, Emre EMMEZ, Hakan FINCAN, Gökçe Öztürk BÖRCEK, Alp Özgün SECEN, Ahmet Eren ERCAN, Sevim |
description | Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers.
To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.
Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.
We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.
Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma. |
doi_str_mv | 10.1227/NEU.0b013e3182262c5b |
format | Article |
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To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.
Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.
We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.
Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.</description><identifier>ISSN: 0148-396X</identifier><identifier>EISSN: 1524-4040</identifier><identifier>DOI: 10.1227/NEU.0b013e3182262c5b</identifier><identifier>PMID: 21629132</identifier><identifier>CODEN: NRSRDY</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Adolescent ; Antineoplastic Agents - pharmacology ; Astrocytoma - complications ; Astrocytoma - pathology ; Biological and medical sciences ; Brain cancer ; Brain Neoplasms - complications ; Brain Neoplasms - pathology ; Cell Count ; Cell culture ; Cell Proliferation - drug effects ; Drug Screening Assays, Antitumor ; Glial Fibrillary Acidic Protein - metabolism ; Humans ; Magnetic Resonance Imaging ; Male ; Medical sciences ; Neurosurgery ; Primary Cell Culture - methods ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Tetrazolium Salts ; Thiazoles ; Thymidine - metabolism ; Tritium ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism</subject><ispartof>Neurosurgery, 2012, Vol.70 (1), p.188-197</ispartof><rights>2015 INIST-CNRS</rights><rights>Copyright © 2011 by the Congress of Neurological Surgeons</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c313t-7a41031e956044ec6af115d75882ebe4f26637ab56d8a04fd8a29fdc239044713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,4025,27928,27929,27930</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25488911$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21629132$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BAGRIACIK, Emin Umit</creatorcontrib><creatorcontrib>BAYKANER, Mustafa Kemali</creatorcontrib><creatorcontrib>YAMAN, Melek</creatorcontrib><creatorcontrib>SIVRIKAYA, Gizem</creatorcontrib><creatorcontrib>DURDAG, Emre</creatorcontrib><creatorcontrib>EMMEZ, Hakan</creatorcontrib><creatorcontrib>FINCAN, Gökçe Öztürk</creatorcontrib><creatorcontrib>BÖRCEK, Alp Özgün</creatorcontrib><creatorcontrib>SECEN, Ahmet Eren</creatorcontrib><creatorcontrib>ERCAN, Sevim</creatorcontrib><title>Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics</title><title>Neurosurgery</title><addtitle>Neurosurgery</addtitle><description>Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers.
To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.
Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.
We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.
Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.</description><subject>Adolescent</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Astrocytoma - complications</subject><subject>Astrocytoma - pathology</subject><subject>Biological and medical sciences</subject><subject>Brain cancer</subject><subject>Brain Neoplasms - complications</subject><subject>Brain Neoplasms - pathology</subject><subject>Cell Count</subject><subject>Cell culture</subject><subject>Cell Proliferation - drug effects</subject><subject>Drug Screening Assays, Antitumor</subject><subject>Glial Fibrillary Acidic Protein - metabolism</subject><subject>Humans</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Neurosurgery</subject><subject>Primary Cell Culture - methods</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Thymidine - metabolism</subject><subject>Tritium</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><issn>0148-396X</issn><issn>1524-4040</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNpdkF1L5DAUhoO46PjxD0QC4mXdnCRNU-9kGD9g2B3WHfGupJlTWmmbmqQL_nu7zKjgzTkX53nfAw8hZ8CugPPs56_F-oqVDAQK0JwrbtNyj8wg5TKRTLJ9MmMgdSJy9XxIjkJ4YQyUzPQBOeSgeA6Cz8i4CNGUbRPqDvtIXUUNXfmmM_6Nrlp0nfND3Vj6bPpYOxOid_Ytus7QObYtXTY9XtOHnj4104X-wTC4PjT_sMcQaHT00XVI5zV2LtbozYBjbGw4IT8q0wY83e1jsr5d_J3fJ8vfdw_zm2ViBYiYZEYCE4B5qpiUaJWpANJNlmrNsURZcaVEZspUbbRhspomz6uN5SKf-AzEMbnY9g7evY4YYvHiRt9PLwsuUqWF1ExMlNxS1rsQPFbFsDVQACv-uy4m18V311PsfFc-lh1uPkMfcifgcgeYYE1bedPbJnxxqdQ6BxDv_WqJLw</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>BAGRIACIK, Emin Umit</creator><creator>BAYKANER, Mustafa Kemali</creator><creator>YAMAN, Melek</creator><creator>SIVRIKAYA, Gizem</creator><creator>DURDAG, Emre</creator><creator>EMMEZ, Hakan</creator><creator>FINCAN, Gökçe Öztürk</creator><creator>BÖRCEK, Alp Özgün</creator><creator>SECEN, Ahmet Eren</creator><creator>ERCAN, Sevim</creator><general>Lippincott Williams & Wilkins</general><general>Wolters Kluwer Health, Inc</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>2012</creationdate><title>Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics</title><author>BAGRIACIK, Emin Umit ; BAYKANER, Mustafa Kemali ; YAMAN, Melek ; SIVRIKAYA, Gizem ; DURDAG, Emre ; EMMEZ, Hakan ; FINCAN, Gökçe Öztürk ; BÖRCEK, Alp Özgün ; SECEN, Ahmet Eren ; ERCAN, Sevim</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c313t-7a41031e956044ec6af115d75882ebe4f26637ab56d8a04fd8a29fdc239044713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Adolescent</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Astrocytoma - complications</topic><topic>Astrocytoma - pathology</topic><topic>Biological and medical sciences</topic><topic>Brain cancer</topic><topic>Brain Neoplasms - complications</topic><topic>Brain Neoplasms - pathology</topic><topic>Cell Count</topic><topic>Cell culture</topic><topic>Cell Proliferation - drug effects</topic><topic>Drug Screening Assays, Antitumor</topic><topic>Glial Fibrillary Acidic Protein - metabolism</topic><topic>Humans</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Neurosurgery</topic><topic>Primary Cell Culture - methods</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Thymidine - metabolism</topic><topic>Tritium</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BAGRIACIK, Emin Umit</creatorcontrib><creatorcontrib>BAYKANER, Mustafa Kemali</creatorcontrib><creatorcontrib>YAMAN, Melek</creatorcontrib><creatorcontrib>SIVRIKAYA, Gizem</creatorcontrib><creatorcontrib>DURDAG, Emre</creatorcontrib><creatorcontrib>EMMEZ, Hakan</creatorcontrib><creatorcontrib>FINCAN, Gökçe Öztürk</creatorcontrib><creatorcontrib>BÖRCEK, Alp Özgün</creatorcontrib><creatorcontrib>SECEN, Ahmet Eren</creatorcontrib><creatorcontrib>ERCAN, Sevim</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Neurosurgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BAGRIACIK, Emin Umit</au><au>BAYKANER, Mustafa Kemali</au><au>YAMAN, Melek</au><au>SIVRIKAYA, Gizem</au><au>DURDAG, Emre</au><au>EMMEZ, Hakan</au><au>FINCAN, Gökçe Öztürk</au><au>BÖRCEK, Alp Özgün</au><au>SECEN, Ahmet Eren</au><au>ERCAN, Sevim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics</atitle><jtitle>Neurosurgery</jtitle><addtitle>Neurosurgery</addtitle><date>2012</date><risdate>2012</risdate><volume>70</volume><issue>1</issue><spage>188</spage><epage>197</epage><pages>188-197</pages><issn>0148-396X</issn><eissn>1524-4040</eissn><coden>NRSRDY</coden><abstract>Anaplastic pleomorphic xanthoastrocytoma is an aggressively growing, malignant, and eventually fatal tumor of the central nervous system. Testing chemotherapeutic drug sensitivity under in vitro conditions would be a useful strategy to determine sensitive or resistant drugs for fatal brain cancers.
To establish primary cell cultures of excised tumor tissue from pleomorphic xanthoastrocytoma-bearing patients and to test their sensitivity against various anticancer chemotherapy drugs.
Prepared suspensions of the excised tumor tissue from a patient who had a recurrent grade 3 pleomorphic xanthoastrocytoma was cultured in culture dishes until cells began to grow. Immunofluorescent and immunohistochemical visualizations were performed using confocal and light microscopy. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay in comparison with ³H-thymidine incorporation assay was used to test cellular toxicity of several anticancer drugs.
We established vigorously growing primary cells of the tumor. Drug sensitivity testing was conducted successfully.
Primary cell cultures of surgically removed tumor tissues may be useful in studies of cancer biology and chemotherapeutic drug sensitivity for recurrent malignant brain tumors, particularly for anaplastic pleomorphic xanthoastrocytoma.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>21629132</pmid><doi>10.1227/NEU.0b013e3182262c5b</doi><tpages>10</tpages></addata></record> |
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subjects | Adolescent Antineoplastic Agents - pharmacology Astrocytoma - complications Astrocytoma - pathology Biological and medical sciences Brain cancer Brain Neoplasms - complications Brain Neoplasms - pathology Cell Count Cell culture Cell Proliferation - drug effects Drug Screening Assays, Antitumor Glial Fibrillary Acidic Protein - metabolism Humans Magnetic Resonance Imaging Male Medical sciences Neurosurgery Primary Cell Culture - methods Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tetrazolium Salts Thiazoles Thymidine - metabolism Tritium Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism |
title | Establishment of a Primary Pleomorphic Xanthoastrocytoma Cell Line: In Vitro Responsiveness to Some Chemotherapeutics |
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