Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer
Background: Despite recent advances in diagnosis and treatment, breast cancer remains a leading cause of death in women worldwide. Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of ln...
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| Veröffentlicht in: | Iranian biomedical journal 2019-09, Vol.23 (5), p.303-311 |
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| container_title | Iranian biomedical journal |
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| creator | Ravanbakhsh Gavgani, Reyhaneh Babaei, Esmaeil Hosseinpourfeizi, Mohammad Ali Fakhrjou, Ashraf Montazeri, Vahid |
| description | Background: Despite recent advances in diagnosis and treatment, breast cancer remains a leading cause of death in women worldwide. Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of lncUSMycN in tumor samples and to evaluate its potential role in the breast cancer cell line. Methods: Real-time polymerase chain reaction was employed to assess lncUSMycN expression in breast tumor tissues and cancer cell lines. Furthermore, small interfering RNA was used to knockdown lncUSMycN. Results: The data showed the significant up-regulation of lncUSMycN in tumor tissues compared to non-tumor specimens (95% CI, p = 0.002). Receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC = 0.70, p < 0.001) for invasive breast ductal carcinoma. Furthermore, lncUSMycN knockdown induced apoptosis and suppressed cellular migration in breast cancer cells (p < 0.01). Conclusion: The findings highlight the pivotal role of lncUSMycN in tumorigenesis, providing a new potential target for breast cancer therapy. |
| doi_str_mv | 10.29252/ibj.23.5.1 |
| format | Article |
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Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of lncUSMycN in tumor samples and to evaluate its potential role in the breast cancer cell line. Methods: Real-time polymerase chain reaction was employed to assess lncUSMycN expression in breast tumor tissues and cancer cell lines. Furthermore, small interfering RNA was used to knockdown lncUSMycN. Results: The data showed the significant up-regulation of lncUSMycN in tumor tissues compared to non-tumor specimens (95% CI, p = 0.002). Receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC = 0.70, p < 0.001) for invasive breast ductal carcinoma. Furthermore, lncUSMycN knockdown induced apoptosis and suppressed cellular migration in breast cancer cells (p < 0.01). Conclusion: The findings highlight the pivotal role of lncUSMycN in tumorigenesis, providing a new potential target for breast cancer therapy.</description><identifier>ISSN: 1028-852X</identifier><identifier>EISSN: 2008-823X</identifier><identifier>DOI: 10.29252/ibj.23.5.1</identifier><language>eng</language><publisher>Tehran: Pasteur Institute of Iran</publisher><subject>Apoptosis ; Biomarkers ; Biotechnology ; Breast cancer ; Cell migration ; Invasiveness ; Non-coding RNA ; Polymerase chain reaction ; siRNA ; Tumor cell lines ; Tumorigenesis ; Tumors</subject><ispartof>Iranian biomedical journal, 2019-09, Vol.23 (5), p.303-311</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1431-eb5f0aab441b8bab5649c10df9135c28deb8532ca3ca239fe71e8c0238306a433</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27924,27925</link.rule.ids></links><search><creatorcontrib>Ravanbakhsh Gavgani, Reyhaneh</creatorcontrib><creatorcontrib>Babaei, Esmaeil</creatorcontrib><creatorcontrib>Hosseinpourfeizi, Mohammad Ali</creatorcontrib><creatorcontrib>Fakhrjou, Ashraf</creatorcontrib><creatorcontrib>Montazeri, Vahid</creatorcontrib><creatorcontrib>Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran</creatorcontrib><creatorcontrib>Department of Thoracic Surgery, Noor-Nejat Hospital, Tabriz, Iran</creatorcontrib><creatorcontrib>Department of Pathology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran</creatorcontrib><title>Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer</title><title>Iranian biomedical journal</title><description>Background: Despite recent advances in diagnosis and treatment, breast cancer remains a leading cause of death in women worldwide. Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of lncUSMycN in tumor samples and to evaluate its potential role in the breast cancer cell line. Methods: Real-time polymerase chain reaction was employed to assess lncUSMycN expression in breast tumor tissues and cancer cell lines. Furthermore, small interfering RNA was used to knockdown lncUSMycN. Results: The data showed the significant up-regulation of lncUSMycN in tumor tissues compared to non-tumor specimens (95% CI, p = 0.002). Receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC = 0.70, p < 0.001) for invasive breast ductal carcinoma. Furthermore, lncUSMycN knockdown induced apoptosis and suppressed cellular migration in breast cancer cells (p < 0.01). Conclusion: The findings highlight the pivotal role of lncUSMycN in tumorigenesis, providing a new potential target for breast cancer therapy.</description><subject>Apoptosis</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Breast cancer</subject><subject>Cell migration</subject><subject>Invasiveness</subject><subject>Non-coding RNA</subject><subject>Polymerase chain reaction</subject><subject>siRNA</subject><subject>Tumor cell lines</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><issn>1028-852X</issn><issn>2008-823X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNotkF9LwzAUxYMoOKdPfoGAj9Ka3Nts6eMs_oNZwTkYvoQ0TSWjJjPtBvv2Vren83s4517OIeSasxRyEHDnqnUKmIqUn5ARMCYTCbg6JSPOYGABq3Ny0XVrxlDw6XREPovWeWd0S7WvqfN05_oY6KLf1nsaGlqGnW3pPPivAX1ShNoN-F7OaOvNcvG6N-Vf6j5a3fW00N7YeJRLctbotrNXRx2T5ePDR_GczN-eXorZPDE8Q57YSjRM6yrLeCUrXYlJlhvO6ibnKAzI2lZSIBiNRgPmjZ1yKw0DlMgmOkMck5vD3U0MP1vb9WodttEPLxWgAAkMh7pjcntwmRi6LtpGbaL71nGvOFP_46lhvCGhhOL4C4P1YI4</recordid><startdate>20190901</startdate><enddate>20190901</enddate><creator>Ravanbakhsh Gavgani, Reyhaneh</creator><creator>Babaei, Esmaeil</creator><creator>Hosseinpourfeizi, Mohammad Ali</creator><creator>Fakhrjou, Ashraf</creator><creator>Montazeri, Vahid</creator><general>Pasteur Institute of Iran</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7T5</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20190901</creationdate><title>Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer</title><author>Ravanbakhsh Gavgani, Reyhaneh ; Babaei, Esmaeil ; Hosseinpourfeizi, Mohammad Ali ; Fakhrjou, Ashraf ; Montazeri, Vahid</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1431-eb5f0aab441b8bab5649c10df9135c28deb8532ca3ca239fe71e8c0238306a433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Breast cancer</topic><topic>Cell migration</topic><topic>Invasiveness</topic><topic>Non-coding RNA</topic><topic>Polymerase chain reaction</topic><topic>siRNA</topic><topic>Tumor cell lines</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ravanbakhsh Gavgani, Reyhaneh</creatorcontrib><creatorcontrib>Babaei, Esmaeil</creatorcontrib><creatorcontrib>Hosseinpourfeizi, Mohammad Ali</creatorcontrib><creatorcontrib>Fakhrjou, Ashraf</creatorcontrib><creatorcontrib>Montazeri, Vahid</creatorcontrib><creatorcontrib>Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran</creatorcontrib><creatorcontrib>Department of Thoracic Surgery, Noor-Nejat Hospital, Tabriz, Iran</creatorcontrib><creatorcontrib>Department of Pathology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Middle East & Africa Database</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Iranian biomedical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ravanbakhsh Gavgani, Reyhaneh</au><au>Babaei, Esmaeil</au><au>Hosseinpourfeizi, Mohammad Ali</au><au>Fakhrjou, Ashraf</au><au>Montazeri, Vahid</au><aucorp>Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran</aucorp><aucorp>Department of Thoracic Surgery, Noor-Nejat Hospital, Tabriz, Iran</aucorp><aucorp>Department of Pathology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer</atitle><jtitle>Iranian biomedical journal</jtitle><date>2019-09-01</date><risdate>2019</risdate><volume>23</volume><issue>5</issue><spage>303</spage><epage>311</epage><pages>303-311</pages><issn>1028-852X</issn><eissn>2008-823X</eissn><abstract>Background: Despite recent advances in diagnosis and treatment, breast cancer remains a leading cause of death in women worldwide. Long non-coding RNAs are a new class of RNA molecules that have been shown to participate in tumorigenesis. The aim of this study was to investigate the expression of lncUSMycN in tumor samples and to evaluate its potential role in the breast cancer cell line. Methods: Real-time polymerase chain reaction was employed to assess lncUSMycN expression in breast tumor tissues and cancer cell lines. Furthermore, small interfering RNA was used to knockdown lncUSMycN. Results: The data showed the significant up-regulation of lncUSMycN in tumor tissues compared to non-tumor specimens (95% CI, p = 0.002). Receiver operating characteristic (ROC) curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC = 0.70, p < 0.001) for invasive breast ductal carcinoma. Furthermore, lncUSMycN knockdown induced apoptosis and suppressed cellular migration in breast cancer cells (p < 0.01). 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| ispartof | Iranian biomedical journal, 2019-09, Vol.23 (5), p.303-311 |
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| subjects | Apoptosis Biomarkers Biotechnology Breast cancer Cell migration Invasiveness Non-coding RNA Polymerase chain reaction siRNA Tumor cell lines Tumorigenesis Tumors |
| title | Clinical and in vitro Study of Novel Long Non-Coding RNA lncUSMycN in Breast Cancer Cancer |
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