Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1
Our study aims to investigate the effect of arsenic trioxide (As 2 O 3 ) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells. Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As 2 O 3 were detected by MTT and q...
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| Veröffentlicht in: | Artificial cells, nanomedicine, and biotechnology nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.1932-1937 |
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| container_issue | 1 |
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| container_title | Artificial cells, nanomedicine, and biotechnology |
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| creator | Zhu, Yanfeng Chen, Weihui Guan, Weiqun Fang, Yihong Hao, Chunfeng |
| description | Our study aims to investigate the effect of arsenic trioxide (As
2
O
3
) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells.
Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As
2
O
3
were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3 μmol/L As
2
O
3
. The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively.
As
2
O
3
of different concentration could inhibit the proliferation of Tca8113 cells and IC
50
value was 3.004 ± 0.2379 μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3 μmol/L As
2
O
3
for 48 h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As
2
O
3
on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells.
As
2
O
3
could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1. |
| doi_str_mv | 10.1080/21691401.2019.1613419 |
| format | Article |
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2
O
3
) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells.
Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As
2
O
3
were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3 μmol/L As
2
O
3
. The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively.
As
2
O
3
of different concentration could inhibit the proliferation of Tca8113 cells and IC
50
value was 3.004 ± 0.2379 μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3 μmol/L As
2
O
3
for 48 h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As
2
O
3
on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells.
As
2
O
3
could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.</description><identifier>ISSN: 2169-1401</identifier><identifier>EISSN: 2169-141X</identifier><identifier>DOI: 10.1080/21691401.2019.1613419</identifier><language>eng</language><publisher>Abingdon: Taylor & Francis</publisher><subject>Apoptosis ; Arsenic ; Arsenic trioxide ; BAX protein ; Bcl-2 protein ; Caspase-3 ; Cell growth ; Cell proliferation ; Flow cytometry ; Gene expression ; Id-1 ; Id1 protein ; mRNA ; proliferation ; Protein expression ; Proteins ; siRNA ; tongue squamous cell carcinoma ; Transfection</subject><ispartof>Artificial cells, nanomedicine, and biotechnology, 2019-12, Vol.47 (1), p.1932-1937</ispartof><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group 2019</rights><rights>2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,2102,27924,27925</link.rule.ids></links><search><creatorcontrib>Zhu, Yanfeng</creatorcontrib><creatorcontrib>Chen, Weihui</creatorcontrib><creatorcontrib>Guan, Weiqun</creatorcontrib><creatorcontrib>Fang, Yihong</creatorcontrib><creatorcontrib>Hao, Chunfeng</creatorcontrib><title>Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1</title><title>Artificial cells, nanomedicine, and biotechnology</title><description>Our study aims to investigate the effect of arsenic trioxide (As
2
O
3
) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells.
Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As
2
O
3
were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3 μmol/L As
2
O
3
. The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively.
As
2
O
3
of different concentration could inhibit the proliferation of Tca8113 cells and IC
50
value was 3.004 ± 0.2379 μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3 μmol/L As
2
O
3
for 48 h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As
2
O
3
on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells.
As
2
O
3
could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.</description><subject>Apoptosis</subject><subject>Arsenic</subject><subject>Arsenic trioxide</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>Caspase-3</subject><subject>Cell growth</subject><subject>Cell proliferation</subject><subject>Flow cytometry</subject><subject>Gene expression</subject><subject>Id-1</subject><subject>Id1 protein</subject><subject>mRNA</subject><subject>proliferation</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>siRNA</subject><subject>tongue squamous cell carcinoma</subject><subject>Transfection</subject><issn>2169-1401</issn><issn>2169-141X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>0YH</sourceid><sourceid>DOA</sourceid><recordid>eNpdkc1q3DAUhU1poCHNIxQE3XTjqa7-bO8aQpsMBLJoAt2JK0uaaOqxXMmmmbevnEm7qDaSLt85cO6pqg9AN0Bb-pmB6kBQ2DAK3QYUcAHdm-p8ndcg4Mfbf28K76rLnPe0nBZUI8V59fP7vNgjiZ5cZXbPSXK7ZcA5jDsypTgE71L5xZHgaAlOcZpjDnnlH3psATjp3TBkYo4kjE_BhBfp_OSIe56Sy3nVFnpra3hfnXkcsrt8vS-qx29fH65v67v7m-311V1tmerm2qMxzlJnQFkvpeVIfYkqRM_bxjPHm0aio8Lblnthm9Y5L5WVICWnSnJ-UW1PvjbiXk8pHDAddcSgXwYx7TSmOfSD04BF33ecSdsKI5QxjeDGsEZ5yil2xevTyats49fi8qwPIa-RcXRxyZoxDp0qu1QF_fgfuo9LGktSzbhkvO0aWA2_nKgw-pgO-DumweoZj0NMPuHYh6w5UL2Wq_-Wq9dy9Wu5_A_6dpXW</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Zhu, Yanfeng</creator><creator>Chen, Weihui</creator><creator>Guan, Weiqun</creator><creator>Fang, Yihong</creator><creator>Hao, Chunfeng</creator><general>Taylor & Francis</general><general>Taylor & Francis Ltd</general><general>Taylor & Francis Group</general><scope>0YH</scope><scope>7X8</scope><scope>DOA</scope></search><sort><creationdate>20191201</creationdate><title>Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1</title><author>Zhu, Yanfeng ; Chen, Weihui ; Guan, Weiqun ; Fang, Yihong ; Hao, Chunfeng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-d269t-fabbed0eb16df55d3a0f10844c387f2e3775ae04fd83f4d78eef56d5155306533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Arsenic</topic><topic>Arsenic trioxide</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>Caspase-3</topic><topic>Cell growth</topic><topic>Cell proliferation</topic><topic>Flow cytometry</topic><topic>Gene expression</topic><topic>Id-1</topic><topic>Id1 protein</topic><topic>mRNA</topic><topic>proliferation</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>siRNA</topic><topic>tongue squamous cell carcinoma</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Yanfeng</creatorcontrib><creatorcontrib>Chen, Weihui</creatorcontrib><creatorcontrib>Guan, Weiqun</creatorcontrib><creatorcontrib>Fang, Yihong</creatorcontrib><creatorcontrib>Hao, Chunfeng</creatorcontrib><collection>Taylor & Francis Open Access</collection><collection>MEDLINE - Academic</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Yanfeng</au><au>Chen, Weihui</au><au>Guan, Weiqun</au><au>Fang, Yihong</au><au>Hao, Chunfeng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1</atitle><jtitle>Artificial cells, nanomedicine, and biotechnology</jtitle><date>2019-12-01</date><risdate>2019</risdate><volume>47</volume><issue>1</issue><spage>1932</spage><epage>1937</epage><pages>1932-1937</pages><issn>2169-1401</issn><eissn>2169-141X</eissn><abstract>Our study aims to investigate the effect of arsenic trioxide (As
2
O
3
) on proliferation and apoptosis of Tca8113 tongue squamous carcinoma cells.
Cell proliferation and the expression of Id-1 mRNA in Tca8113 cells after treatment with different concentrations of As
2
O
3
were detected by MTT and qRT-PCR, respectively. The expression of Id-1, cell proliferation and apoptosis in Id-1 silencing Tca8113 cells were detected by qRT-PCR, Western blot, MTT and flow cytometry, respectively. The pcDNA 3.1-Id-1 overexpression vector was transfected into Tca8113 cells combination with 3 μmol/L As
2
O
3
. The detection of cell proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in transfected Tca8113 cells were performed by MTT, flow cytometry and Western blot assay, respectively.
As
2
O
3
of different concentration could inhibit the proliferation of Tca8113 cells and IC
50
value was 3.004 ± 0.2379 μmol/L. The expression of Id-1 mRNA was down-regulated in Tca8113 cells treated with 3 μmol/L As
2
O
3
for 48 h. The results of qRT-PCR, Western blot, MTT and flow cytometry indicated that the expression level of Id-1 and cell proliferation ability were decreased while the apoptosis rate was increased in Tca8113 cells after transfection of Id-1 siRNA. Overexpression of Id-1 could attenuate the inhibition or promotion of As
2
O
3
on proliferation, apoptosis and Caspase-3, Bax and Bcl-2 protein expression in Tca8113 cells.
As
2
O
3
could regulate the proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1.</abstract><cop>Abingdon</cop><pub>Taylor & Francis</pub><doi>10.1080/21691401.2019.1613419</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Alma/SFX Local Collection |
| subjects | Apoptosis Arsenic Arsenic trioxide BAX protein Bcl-2 protein Caspase-3 Cell growth Cell proliferation Flow cytometry Gene expression Id-1 Id1 protein mRNA proliferation Protein expression Proteins siRNA tongue squamous cell carcinoma Transfection |
| title | Study of As2O3 regulating proliferation and apoptosis of Tca8113 cells by inhibiting the expression of Id-1 |
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