Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress
S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma ce...
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| Veröffentlicht in: | Journal of experimental botany 2006-01, Vol.57 (8), p.1785-1793 |
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| creator | Barroso, Juan B Corpas, Francisco J Carreras, Alfonso Rodríguez-Serrano, María Esteban, Francisco J Fernández-Ocaña, Ana Chaki, Mounira Romero-Puertas, María C Valderrama, Raquel Sandalio, Luisa M Río, Luis A. del |
| description | S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 μM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress. |
| doi_str_mv | 10.1093/jxb/erj175 |
| format | Article |
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In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 μM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/erj175</identifier><identifier>PMID: 16595575</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Abiotic stress ; Cadmium ; Cadmium - metabolism ; Collenchyma ; Dehydrogenases ; Down-Regulation ; field crops ; formaldehyde dehydrogenase ; Gels ; immunohistochemistry ; Leaves ; metabolism ; metabolites ; Molecular Sequence Data ; nitric oxide ; oligopeptides ; Oxidation-Reduction ; Oxidative stress ; Oxides ; oxidoreductases ; Peas ; phytotoxicity ; Pisum sativum ; Pisum sativum - enzymology ; Pisum sativum - metabolism ; Plant cells ; Plant Leaves - enzymology ; Plant Leaves - metabolism ; plant physiology ; Plant Proteins - metabolism ; plant stress ; Plants ; reactive nitrogen species ; RNS ; S-nitrosoglutathione ; S-Nitrosoglutathione - metabolism ; S-nitrosoglutathione reductase ; signalling</subject><ispartof>Journal of experimental botany, 2006-01, Vol.57 (8), p.1785-1793</ispartof><rights>Society for Experimental Biology 2006</rights><rights>Copyright Oxford University Press(England) May 2006</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c432t-9923979aa794b6b8588d295598e802ce6ff61556e5d6dba88981c70d6d7fcd903</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24036137$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24036137$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16595575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barroso, Juan B</creatorcontrib><creatorcontrib>Corpas, Francisco J</creatorcontrib><creatorcontrib>Carreras, Alfonso</creatorcontrib><creatorcontrib>Rodríguez-Serrano, María</creatorcontrib><creatorcontrib>Esteban, Francisco J</creatorcontrib><creatorcontrib>Fernández-Ocaña, Ana</creatorcontrib><creatorcontrib>Chaki, Mounira</creatorcontrib><creatorcontrib>Romero-Puertas, María C</creatorcontrib><creatorcontrib>Valderrama, Raquel</creatorcontrib><creatorcontrib>Sandalio, Luisa M</creatorcontrib><creatorcontrib>Río, Luis A. del</creatorcontrib><title>Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 μM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress.</description><subject>Abiotic stress</subject><subject>Cadmium</subject><subject>Cadmium - metabolism</subject><subject>Collenchyma</subject><subject>Dehydrogenases</subject><subject>Down-Regulation</subject><subject>field crops</subject><subject>formaldehyde dehydrogenase</subject><subject>Gels</subject><subject>immunohistochemistry</subject><subject>Leaves</subject><subject>metabolism</subject><subject>metabolites</subject><subject>Molecular Sequence Data</subject><subject>nitric oxide</subject><subject>oligopeptides</subject><subject>Oxidation-Reduction</subject><subject>Oxidative stress</subject><subject>Oxides</subject><subject>oxidoreductases</subject><subject>Peas</subject><subject>phytotoxicity</subject><subject>Pisum sativum</subject><subject>Pisum sativum - enzymology</subject><subject>Pisum sativum - metabolism</subject><subject>Plant cells</subject><subject>Plant Leaves - enzymology</subject><subject>Plant Leaves - metabolism</subject><subject>plant physiology</subject><subject>Plant Proteins - metabolism</subject><subject>plant stress</subject><subject>Plants</subject><subject>reactive nitrogen species</subject><subject>RNS</subject><subject>S-nitrosoglutathione</subject><subject>S-Nitrosoglutathione - metabolism</subject><subject>S-nitrosoglutathione reductase</subject><subject>signalling</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFP3DAQhS3Uqiy0l95LLY6VAmM7tuMjArZUWpUDIBAXy4kdSLobB9uRtv31NcqK3noazbxPb2YeQp8JnBBQ7LTf1qcu9ETyPbQgpYCCloy8QwsASgtQXO6jgxh7AODA-Qe0TwRXnEu-QNuVb8y6-2NS5wfsW3xTDF0KPvqn9ZRMes5jh81gsduOwcX4Pyw4OzXJRIe7AY_O4HFthhTxNFgXcGPspps2OKZXn4_ofWvW0X3a1UN0t7y8Pb8qVtfff5yfrYqmZDQVSlGmpDJGqrIWdcWrytJ8u6pcBbRxom0F4Vw4boWtTVWpijQSciPbxipgh-h49h2Df5lcTLr3UxjySk0ZB0JLVWbo2ww1-aUYXKvH0G1M-K0J6NeMdc5Yzxln-GjnONUbZ_-hu1Az8GUG-ph8eNNpCUwQJrNezHoXk9u-6Sb80kIyyfXVw6O-Xy7lz-UD1xeZ_zrzrfHaPIUu6rsbCoQBAak4UewvrQGcqQ</recordid><startdate>20060101</startdate><enddate>20060101</enddate><creator>Barroso, Juan B</creator><creator>Corpas, Francisco J</creator><creator>Carreras, Alfonso</creator><creator>Rodríguez-Serrano, María</creator><creator>Esteban, Francisco J</creator><creator>Fernández-Ocaña, Ana</creator><creator>Chaki, Mounira</creator><creator>Romero-Puertas, María C</creator><creator>Valderrama, Raquel</creator><creator>Sandalio, Luisa M</creator><creator>Río, Luis A. del</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20060101</creationdate><title>Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress</title><author>Barroso, Juan B ; Corpas, Francisco J ; Carreras, Alfonso ; Rodríguez-Serrano, María ; Esteban, Francisco J ; Fernández-Ocaña, Ana ; Chaki, Mounira ; Romero-Puertas, María C ; Valderrama, Raquel ; Sandalio, Luisa M ; Río, Luis A. del</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-9923979aa794b6b8588d295598e802ce6ff61556e5d6dba88981c70d6d7fcd903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Abiotic stress</topic><topic>Cadmium</topic><topic>Cadmium - metabolism</topic><topic>Collenchyma</topic><topic>Dehydrogenases</topic><topic>Down-Regulation</topic><topic>field crops</topic><topic>formaldehyde dehydrogenase</topic><topic>Gels</topic><topic>immunohistochemistry</topic><topic>Leaves</topic><topic>metabolism</topic><topic>metabolites</topic><topic>Molecular Sequence Data</topic><topic>nitric oxide</topic><topic>oligopeptides</topic><topic>Oxidation-Reduction</topic><topic>Oxidative stress</topic><topic>Oxides</topic><topic>oxidoreductases</topic><topic>Peas</topic><topic>phytotoxicity</topic><topic>Pisum sativum</topic><topic>Pisum sativum - enzymology</topic><topic>Pisum sativum - metabolism</topic><topic>Plant cells</topic><topic>Plant Leaves - enzymology</topic><topic>Plant Leaves - metabolism</topic><topic>plant physiology</topic><topic>Plant Proteins - metabolism</topic><topic>plant stress</topic><topic>Plants</topic><topic>reactive nitrogen species</topic><topic>RNS</topic><topic>S-nitrosoglutathione</topic><topic>S-Nitrosoglutathione - metabolism</topic><topic>S-nitrosoglutathione reductase</topic><topic>signalling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barroso, Juan B</creatorcontrib><creatorcontrib>Corpas, Francisco J</creatorcontrib><creatorcontrib>Carreras, Alfonso</creatorcontrib><creatorcontrib>Rodríguez-Serrano, María</creatorcontrib><creatorcontrib>Esteban, Francisco J</creatorcontrib><creatorcontrib>Fernández-Ocaña, Ana</creatorcontrib><creatorcontrib>Chaki, Mounira</creatorcontrib><creatorcontrib>Romero-Puertas, María C</creatorcontrib><creatorcontrib>Valderrama, Raquel</creatorcontrib><creatorcontrib>Sandalio, Luisa M</creatorcontrib><creatorcontrib>Río, Luis A. del</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barroso, Juan B</au><au>Corpas, Francisco J</au><au>Carreras, Alfonso</au><au>Rodríguez-Serrano, María</au><au>Esteban, Francisco J</au><au>Fernández-Ocaña, Ana</au><au>Chaki, Mounira</au><au>Romero-Puertas, María C</au><au>Valderrama, Raquel</au><au>Sandalio, Luisa M</au><au>Río, Luis A. del</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2006-01-01</date><risdate>2006</risdate><volume>57</volume><issue>8</issue><spage>1785</spage><epage>1793</epage><pages>1785-1793</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>S-nitrosoglutathione (GSNO) is considered a natural nitric oxide (NO·) reservoir and a reactive nitrogen intermediate in animal cells, but little is known about this molecule and its metabolism in plant systems. In this work, using pea plants as a model system, the presence of GSNO in collenchyma cells was demonstrated by an immunohistochemical method. When pea plants were grown with a toxic Cd concentration (50 μM) the content of GSNO in collenchyma cells was drastically reduced. Determination of the nitric oxide (NO·) and gluthathione contents in leaves by confocal laser scanning microscopy and HPLC, respectively, showed a marked decrease of both compounds in plants treated with cadmium. The analysis of the S-nitrosoglutathione reductase (GSNOR) activity and its transcript expression in leaves showed a reduction of 31% by cadmium. These results indicate that GSNO is associated with a specific plant cell type, and this metabolite and its related catabolic activity, GSNOR, are both down-regulated under Cd stress.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>16595575</pmid><doi>10.1093/jxb/erj175</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; Oxford University Press Journals All Titles (1996-Current); MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Abiotic stress Cadmium Cadmium - metabolism Collenchyma Dehydrogenases Down-Regulation field crops formaldehyde dehydrogenase Gels immunohistochemistry Leaves metabolism metabolites Molecular Sequence Data nitric oxide oligopeptides Oxidation-Reduction Oxidative stress Oxides oxidoreductases Peas phytotoxicity Pisum sativum Pisum sativum - enzymology Pisum sativum - metabolism Plant cells Plant Leaves - enzymology Plant Leaves - metabolism plant physiology Plant Proteins - metabolism plant stress Plants reactive nitrogen species RNS S-nitrosoglutathione S-Nitrosoglutathione - metabolism S-nitrosoglutathione reductase signalling |
| title | Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress |
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