The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions
The design and scalable synthesis of robust 2D biological ultrathin films with a tunable structure and function and the ability to be easily transferred to a range of substrates remain key challenges in chemistry and materials science. Herein, we report the use of the thiol–disulfide exchange reacti...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2020-02, Vol.59 (7), p.2850-2859 |
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| creator | Xu, Yan Liu, Yongchun Hu, Xinyi Qin, Rongrong Su, Hao Li, Juling Yang, Peng |
| description | The design and scalable synthesis of robust 2D biological ultrathin films with a tunable structure and function and the ability to be easily transferred to a range of substrates remain key challenges in chemistry and materials science. Herein, we report the use of the thiol–disulfide exchange reaction in the synthesis of a macroscopic 2D ultrathin proteinaceous film with the potential for large‐scale fabrication and on‐demand encapsulation/release of functional molecules. The reaction between the Cys6–Cys127 disulfide bond of lysozyme and cysteine is chemo‐ and site‐selective. The partially unfolded lysozyme–cysteine monomers aggregate at the air/water or solid/liquid interface to form an ultra‐large 2D nanofilm (900 cm2) with about 100 % optical transparency. This material adheres to a wide range of substrates and encapsulates and releases a range of molecules without significantly affecting activity.
The thiol–disulfide exchange reaction between cysteine and lysozyme is used to produce a 2D, thin proteinaceous film with approximately 100 % optical transparency. This film adheres to a range of surfaces and can be used to encapsulate and release functional molecules, such as proteins. |
| doi_str_mv | 10.1002/anie.201912848 |
| format | Article |
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The thiol–disulfide exchange reaction between cysteine and lysozyme is used to produce a 2D, thin proteinaceous film with approximately 100 % optical transparency. This film adheres to a range of surfaces and can be used to encapsulate and release functional molecules, such as proteins.</description><subject>aggregation</subject><subject>Chemical synthesis</subject><subject>Cysteine</subject><subject>Disulfides - chemistry</subject><subject>Encapsulation</subject><subject>Exchanging</subject><subject>Fabrication</subject><subject>Lysozyme</subject><subject>Macromolecular Substances - chemical synthesis</subject><subject>Macromolecular Substances - chemistry</subject><subject>Materials science</subject><subject>Models, Molecular</subject><subject>Molecular Structure</subject><subject>Monomers</subject><subject>Nanoparticles - chemistry</subject><subject>Organic chemistry</subject><subject>Particle Size</subject><subject>proteins</subject><subject>Proteins - chemical synthesis</subject><subject>Proteins - chemistry</subject><subject>redox chemistry</subject><subject>Structure-function relationships</subject><subject>Substrates</subject><subject>Sulfhydryl Compounds - chemistry</subject><subject>Surface Properties</subject><subject>Thin films</subject><issn>1433-7851</issn><issn>1521-3773</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9P2zAUgC20aTDYdcfJ0s7p_COJnSMqBSpVMIn2HDnOC3GV2J2dsPXGnQvS_sP-JXNVYEdOftL73mfpQ-grJRNKCPuhrIEJI7SgTKbyCJ3QjNGEC8E_xDnlPBEyo8focwjryEtJ8k_omFNJWE74CXpatoDvtnZoIZiAXYMVZhd41Q1e7R6fF8rfA_7p3QDG4rtx41XvOtBjpzy-UdY1putxtcXTFnoXIK4G8wB42RrX7R7_Xpgwdo2pAc_-6FbZKFO2xmYIeNZDdNsBX442HjkbztDHRnUBvry8p2h1OVtOr5PF7dV8er5IdEqJTHRRN5kiXFSSiUJlRIMgIk1zruoc4k4AS5WAVKpMNzyvgVa6LuqKaAL7Mqfo-8G78e7XCGEo1270Nn5ZMp4RkjGZy0hNDpT2LgQPTbnxpld-W1JS7tuX-_blW_t48O1FO1Y91G_4a-wIFAfgt-lg-46uPL-Zz_7L_wEa75R6</recordid><startdate>20200210</startdate><enddate>20200210</enddate><creator>Xu, Yan</creator><creator>Liu, Yongchun</creator><creator>Hu, Xinyi</creator><creator>Qin, Rongrong</creator><creator>Su, Hao</creator><creator>Li, Juling</creator><creator>Yang, Peng</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>K9.</scope><orcidid>https://orcid.org/0000-0002-0463-1024</orcidid></search><sort><creationdate>20200210</creationdate><title>The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions</title><author>Xu, Yan ; Liu, Yongchun ; Hu, Xinyi ; Qin, Rongrong ; Su, Hao ; Li, Juling ; Yang, Peng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4108-c9df5a037b8279a50ce7074463ad6edf57e24a7e48a5cf36de1bcd9db0c0e3773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>aggregation</topic><topic>Chemical synthesis</topic><topic>Cysteine</topic><topic>Disulfides - chemistry</topic><topic>Encapsulation</topic><topic>Exchanging</topic><topic>Fabrication</topic><topic>Lysozyme</topic><topic>Macromolecular Substances - chemical synthesis</topic><topic>Macromolecular Substances - chemistry</topic><topic>Materials science</topic><topic>Models, Molecular</topic><topic>Molecular Structure</topic><topic>Monomers</topic><topic>Nanoparticles - chemistry</topic><topic>Organic chemistry</topic><topic>Particle Size</topic><topic>proteins</topic><topic>Proteins - chemical synthesis</topic><topic>Proteins - chemistry</topic><topic>redox chemistry</topic><topic>Structure-function relationships</topic><topic>Substrates</topic><topic>Sulfhydryl Compounds - chemistry</topic><topic>Surface Properties</topic><topic>Thin films</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Yan</creatorcontrib><creatorcontrib>Liu, Yongchun</creatorcontrib><creatorcontrib>Hu, Xinyi</creatorcontrib><creatorcontrib>Qin, Rongrong</creatorcontrib><creatorcontrib>Su, Hao</creatorcontrib><creatorcontrib>Li, Juling</creatorcontrib><creatorcontrib>Yang, Peng</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><jtitle>Angewandte Chemie International Edition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Yan</au><au>Liu, Yongchun</au><au>Hu, Xinyi</au><au>Qin, Rongrong</au><au>Su, Hao</au><au>Li, Juling</au><au>Yang, Peng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions</atitle><jtitle>Angewandte Chemie International Edition</jtitle><addtitle>Angew Chem Int Ed Engl</addtitle><date>2020-02-10</date><risdate>2020</risdate><volume>59</volume><issue>7</issue><spage>2850</spage><epage>2859</epage><pages>2850-2859</pages><issn>1433-7851</issn><eissn>1521-3773</eissn><abstract>The design and scalable synthesis of robust 2D biological ultrathin films with a tunable structure and function and the ability to be easily transferred to a range of substrates remain key challenges in chemistry and materials science. Herein, we report the use of the thiol–disulfide exchange reaction in the synthesis of a macroscopic 2D ultrathin proteinaceous film with the potential for large‐scale fabrication and on‐demand encapsulation/release of functional molecules. The reaction between the Cys6–Cys127 disulfide bond of lysozyme and cysteine is chemo‐ and site‐selective. The partially unfolded lysozyme–cysteine monomers aggregate at the air/water or solid/liquid interface to form an ultra‐large 2D nanofilm (900 cm2) with about 100 % optical transparency. This material adheres to a wide range of substrates and encapsulates and releases a range of molecules without significantly affecting activity.
The thiol–disulfide exchange reaction between cysteine and lysozyme is used to produce a 2D, thin proteinaceous film with approximately 100 % optical transparency. This film adheres to a range of surfaces and can be used to encapsulate and release functional molecules, such as proteins.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>31802603</pmid><doi>10.1002/anie.201912848</doi><tpages>10</tpages><edition>International ed. in English</edition><orcidid>https://orcid.org/0000-0002-0463-1024</orcidid></addata></record> |
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| subjects | aggregation Chemical synthesis Cysteine Disulfides - chemistry Encapsulation Exchanging Fabrication Lysozyme Macromolecular Substances - chemical synthesis Macromolecular Substances - chemistry Materials science Models, Molecular Molecular Structure Monomers Nanoparticles - chemistry Organic chemistry Particle Size proteins Proteins - chemical synthesis Proteins - chemistry redox chemistry Structure-function relationships Substrates Sulfhydryl Compounds - chemistry Surface Properties Thin films |
| title | The Synthesis of a 2D Ultra‐Large Protein Supramolecular Nanofilm by Chemoselective Thiol–Disulfide Exchange and its Emergent Functions |
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