Cell and Molecular Biology, Biochemistry and Molecular Physiology. Intracellular pH regulation in maize root tips exposed to ammonium at high external pH

Cell and Molecular Biology, Biochemistry and Molecular Physiology. Intracellular pH regulation in maize root tips exposed to ammonium at high external pH

Ammonium-induced changes in the cytoplasmic and vacuolar pH values of excised maize (Zea mays L.) root tips, measured by in vivo

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Veröffentlicht in:Journal of experimental botany 2000-02, Vol.51 (343), p.207
Hauptverfasser: Gerendas, J, Ratcliffe, R G
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Biochemistry
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container_title Journal of experimental botany
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creator Gerendas, J
Ratcliffe, R G
description Ammonium-induced changes in the cytoplasmic and vacuolar pH values of excised maize (Zea mays L.) root tips, measured by in vivo
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Intracellular pH regulation in maize root tips exposed to ammonium at high external pH</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>JSTOR Archive Collection A-Z Listing</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Gerendas, J ; Ratcliffe, R G</creator><creatorcontrib>Gerendas, J ; Ratcliffe, R G</creatorcontrib><description><![CDATA[Ammonium-induced changes in the cytoplasmic and vacuolar pH values of excised maize (Zea mays L.) root tips, measured by in vivo <31<P nuclear magnetic resonance (NMR) spectroscopy, were correlated with the ammonium content of the tissue, determined by <14<N NMR. Calculations based on these measurements indicated that the pH changes observed during exposure to 10 mM ammonium for 1 h at pH 9.0, and in the recovery following the removal of the external ammonium supply, were largely determined by the influx and efflux of the weak base NH<3<. Carboxylate synthesis, detected by both in vivo <13<C NMR and the incorporation of [<14<C]bicarbonate, was stimulated by the ammonium-induced alkalinization of the root tips, but the contribution that this proton-generating process made to pH regulation during and after the ammonium treatment was quantitatively insignificant. Similarly, ammonium assimilation, which was shown to occur via the proton-generating glutamine synthetase/glutamate synthase pathway using in vivo <15<N NMR, was also quantitatively insignificant in comparison with the large changes in ammonium content that occurred during the ammonium treatment and subsequent recovery. The results are discussed in relation to several recent studies in which ammonium was used to perturb intracellular pH values, and it is argued (i) that a new method for probing the subcellular compartmentation of amino acids, based on an ammonium-induced alkalinization of the cytoplasm may be difficult to implement in dense heterogeneous tissues; and (ii) that observations on the apparently proton-consuming effect of ammonium assimilation in rice root hairs may actually reflect unusually rapid assimilation.]]></description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford Publishing Limited (England)</publisher><subject>Biochemistry</subject><ispartof>Journal of experimental botany, 2000-02, Vol.51 (343), p.207</ispartof><rights>Copyright Oxford University Press(England) Feb 2000</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Gerendas, J</creatorcontrib><creatorcontrib>Ratcliffe, R G</creatorcontrib><title>Cell and Molecular Biology, Biochemistry and Molecular Physiology. Intracellular pH regulation in maize root tips exposed to ammonium at high external pH</title><title>Journal of experimental botany</title><description><![CDATA[Ammonium-induced changes in the cytoplasmic and vacuolar pH values of excised maize (Zea mays L.) root tips, measured by in vivo <31<P nuclear magnetic resonance (NMR) spectroscopy, were correlated with the ammonium content of the tissue, determined by <14<N NMR. Calculations based on these measurements indicated that the pH changes observed during exposure to 10 mM ammonium for 1 h at pH 9.0, and in the recovery following the removal of the external ammonium supply, were largely determined by the influx and efflux of the weak base NH<3<. Carboxylate synthesis, detected by both in vivo <13<C NMR and the incorporation of [<14<C]bicarbonate, was stimulated by the ammonium-induced alkalinization of the root tips, but the contribution that this proton-generating process made to pH regulation during and after the ammonium treatment was quantitatively insignificant. Similarly, ammonium assimilation, which was shown to occur via the proton-generating glutamine synthetase/glutamate synthase pathway using in vivo <15<N NMR, was also quantitatively insignificant in comparison with the large changes in ammonium content that occurred during the ammonium treatment and subsequent recovery. The results are discussed in relation to several recent studies in which ammonium was used to perturb intracellular pH values, and it is argued (i) that a new method for probing the subcellular compartmentation of amino acids, based on an ammonium-induced alkalinization of the cytoplasm may be difficult to implement in dense heterogeneous tissues; and (ii) that observations on the apparently proton-consuming effect of ammonium assimilation in rice root hairs may actually reflect unusually rapid assimilation.]]></description><subject>Biochemistry</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqNjMtKxEAQRRtRMD7-oXBtpJJM-9g6KONiwIX7ocmUSQ_dXbGrAsY_8W_NqCtXru6Bc7gHpqgW11jWi6Y6NAViXZd4Z2-OzYnIDhEtWluYzyWFAC5tYc2B2jG4DPeeA3fT5R7anqIXzdOf5rmf5Ce7gqek2bXzz7cZVpCpm1E9J_AJovMfBJlZQf0gQO8DC21BGVyMnPwYwSn0vutnp5STC_PLmTl6dUHo_HdPzcXjw8tyVQ6Z30YS3ex43LeyqRuL2OBt1fwr-gLAQlq-</recordid><startdate>20000201</startdate><enddate>20000201</enddate><creator>Gerendas, J</creator><creator>Ratcliffe, R G</creator><general>Oxford Publishing Limited (England)</general><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20000201</creationdate><title>Cell and Molecular Biology, Biochemistry and Molecular Physiology. 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Carboxylate synthesis, detected by both in vivo <13<C NMR and the incorporation of [<14<C]bicarbonate, was stimulated by the ammonium-induced alkalinization of the root tips, but the contribution that this proton-generating process made to pH regulation during and after the ammonium treatment was quantitatively insignificant. Similarly, ammonium assimilation, which was shown to occur via the proton-generating glutamine synthetase/glutamate synthase pathway using in vivo <15<N NMR, was also quantitatively insignificant in comparison with the large changes in ammonium content that occurred during the ammonium treatment and subsequent recovery. The results are discussed in relation to several recent studies in which ammonium was used to perturb intracellular pH values, and it is argued (i) that a new method for probing the subcellular compartmentation of amino acids, based on an ammonium-induced alkalinization of the cytoplasm may be difficult to implement in dense heterogeneous tissues; and (ii) that observations on the apparently proton-consuming effect of ammonium assimilation in rice root hairs may actually reflect unusually rapid assimilation.]]></abstract><cop>Oxford</cop><pub>Oxford Publishing Limited (England)</pub></addata></record>
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source Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection
subjects Biochemistry
title Cell and Molecular Biology, Biochemistry and Molecular Physiology. Intracellular pH regulation in maize root tips exposed to ammonium at high external pH
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