Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail
A specific macroalgal biomass decomposing fungus SL1 newly isolated and identified as Aspergillus fumigatus was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific acti...
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| Veröffentlicht in: | Waste and biomass valorization 2020, Vol.11 (1), p.211-222 |
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| creator | Ben Yahmed, Nesrine Berrejeb, Nadia Jmel, Mohamed Amine Jazzar, Souhir Marzouki, M. Nejib Smaali, Issam |
| description | A specific macroalgal biomass decomposing fungus SL1 newly isolated and identified as
Aspergillus fumigatus
was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific activities of 30 and 33 U/mg of proteins for endoglucanase and β-glucosidase, respectively) different from commercial ones as shown by zymography. The application of the produced cellulases-based enzymatic cocktail for the saccharification of alkali pretreated
Ulva
sp. biomass yielded of 58%. This saccharification rate was optimized using response surface methodology (RSM). An increase of 36% in saccharification yield was obtained under optimized conditions (13 U CMCase, 4% substrate and 135 rpm agitation) which agreed with model predictions. The biocatalytic conversion using specific fungal cellulases may be a promising approach for the biodegradation of stranded macroalgae and its valorization mostly for bioethanol production. |
| doi_str_mv | 10.1007/s12649-018-0397-4 |
| format | Article |
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Aspergillus fumigatus
was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific activities of 30 and 33 U/mg of proteins for endoglucanase and β-glucosidase, respectively) different from commercial ones as shown by zymography. The application of the produced cellulases-based enzymatic cocktail for the saccharification of alkali pretreated
Ulva
sp. biomass yielded of 58%. This saccharification rate was optimized using response surface methodology (RSM). An increase of 36% in saccharification yield was obtained under optimized conditions (13 U CMCase, 4% substrate and 135 rpm agitation) which agreed with model predictions. The biocatalytic conversion using specific fungal cellulases may be a promising approach for the biodegradation of stranded macroalgae and its valorization mostly for bioethanol production.</description><identifier>ISSN: 1877-2641</identifier><identifier>EISSN: 1877-265X</identifier><identifier>DOI: 10.1007/s12649-018-0397-4</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Algae ; Biodegradation ; Biofuels ; Biomass ; Carbon sources ; Cellobiase ; Cellulase ; Conversion ; Endoglucanase ; Engineering ; Environment ; Environmental Engineering/Biotechnology ; Ethanol ; Fungi ; Glucosidase ; Industrial Pollution Prevention ; Original Paper ; Renewable and Green Energy ; Response surface methodology ; Saccharification ; Substrates ; Waste Management/Waste Technology ; β-Glucosidase</subject><ispartof>Waste and biomass valorization, 2020, Vol.11 (1), p.211-222</ispartof><rights>Springer Nature B.V. 2018</rights><rights>2018© Springer Nature B.V. 2018</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-5b62152844f08bed5672d8f4bc237a11f89db4102b38a35bdde0a9ac2b76780a3</citedby><cites>FETCH-LOGICAL-c353t-5b62152844f08bed5672d8f4bc237a11f89db4102b38a35bdde0a9ac2b76780a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12649-018-0397-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12649-018-0397-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Ben Yahmed, Nesrine</creatorcontrib><creatorcontrib>Berrejeb, Nadia</creatorcontrib><creatorcontrib>Jmel, Mohamed Amine</creatorcontrib><creatorcontrib>Jazzar, Souhir</creatorcontrib><creatorcontrib>Marzouki, M. Nejib</creatorcontrib><creatorcontrib>Smaali, Issam</creatorcontrib><title>Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail</title><title>Waste and biomass valorization</title><addtitle>Waste Biomass Valor</addtitle><description>A specific macroalgal biomass decomposing fungus SL1 newly isolated and identified as
Aspergillus fumigatus
was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific activities of 30 and 33 U/mg of proteins for endoglucanase and β-glucosidase, respectively) different from commercial ones as shown by zymography. The application of the produced cellulases-based enzymatic cocktail for the saccharification of alkali pretreated
Ulva
sp. biomass yielded of 58%. This saccharification rate was optimized using response surface methodology (RSM). An increase of 36% in saccharification yield was obtained under optimized conditions (13 U CMCase, 4% substrate and 135 rpm agitation) which agreed with model predictions. The biocatalytic conversion using specific fungal cellulases may be a promising approach for the biodegradation of stranded macroalgae and its valorization mostly for bioethanol production.</description><subject>Algae</subject><subject>Biodegradation</subject><subject>Biofuels</subject><subject>Biomass</subject><subject>Carbon sources</subject><subject>Cellobiase</subject><subject>Cellulase</subject><subject>Conversion</subject><subject>Endoglucanase</subject><subject>Engineering</subject><subject>Environment</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Ethanol</subject><subject>Fungi</subject><subject>Glucosidase</subject><subject>Industrial Pollution Prevention</subject><subject>Original Paper</subject><subject>Renewable and Green Energy</subject><subject>Response surface methodology</subject><subject>Saccharification</subject><subject>Substrates</subject><subject>Waste Management/Waste Technology</subject><subject>β-Glucosidase</subject><issn>1877-2641</issn><issn>1877-265X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kE1Lw0AQhhdRsNT-AG8LnqP7lWRztKVWoeKhFrwtk_0oW7dJu5sK_fcmVPTkZWYO7_vMzIvQLSX3lJDyIVFWiCojVGaEV2UmLtCIyrLMWJF_XP7Ogl6jSUpbQgijVDJejtBh7pzX3jYdnvpWQwfh1HmNZ23zZWPybYNbh1ddhMZYgxfR2ga_go4thA2EwbSDlPA6-WaDAa_2Vns3AGwIxwDJpmzaV9MT9WcHPtygKwch2clPH6P10_x99pwt3xYvs8dlpnnOuyyvC0ZzJoVwRNbW5EXJjHSi1v3dQKmTlakFJazmEnheG2MJVKBZXRalJMDH6O7M3cf2cLSpU9v2GJt-pWJccCZJXoleRc-q_qOUonVqH_0O4klRooZw1Tlc1YerhnDV4GFnT-q1zcbGP_L_pm-oIXzl</recordid><startdate>2020</startdate><enddate>2020</enddate><creator>Ben Yahmed, Nesrine</creator><creator>Berrejeb, Nadia</creator><creator>Jmel, Mohamed Amine</creator><creator>Jazzar, Souhir</creator><creator>Marzouki, M. Nejib</creator><creator>Smaali, Issam</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>2020</creationdate><title>Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail</title><author>Ben Yahmed, Nesrine ; Berrejeb, Nadia ; Jmel, Mohamed Amine ; Jazzar, Souhir ; Marzouki, M. Nejib ; Smaali, Issam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-5b62152844f08bed5672d8f4bc237a11f89db4102b38a35bdde0a9ac2b76780a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Algae</topic><topic>Biodegradation</topic><topic>Biofuels</topic><topic>Biomass</topic><topic>Carbon sources</topic><topic>Cellobiase</topic><topic>Cellulase</topic><topic>Conversion</topic><topic>Endoglucanase</topic><topic>Engineering</topic><topic>Environment</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Ethanol</topic><topic>Fungi</topic><topic>Glucosidase</topic><topic>Industrial Pollution Prevention</topic><topic>Original Paper</topic><topic>Renewable and Green Energy</topic><topic>Response surface methodology</topic><topic>Saccharification</topic><topic>Substrates</topic><topic>Waste Management/Waste Technology</topic><topic>β-Glucosidase</topic><toplevel>online_resources</toplevel><creatorcontrib>Ben Yahmed, Nesrine</creatorcontrib><creatorcontrib>Berrejeb, Nadia</creatorcontrib><creatorcontrib>Jmel, Mohamed Amine</creatorcontrib><creatorcontrib>Jazzar, Souhir</creatorcontrib><creatorcontrib>Marzouki, M. Nejib</creatorcontrib><creatorcontrib>Smaali, Issam</creatorcontrib><collection>CrossRef</collection><jtitle>Waste and biomass valorization</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ben Yahmed, Nesrine</au><au>Berrejeb, Nadia</au><au>Jmel, Mohamed Amine</au><au>Jazzar, Souhir</au><au>Marzouki, M. Nejib</au><au>Smaali, Issam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail</atitle><jtitle>Waste and biomass valorization</jtitle><stitle>Waste Biomass Valor</stitle><date>2020</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>211</spage><epage>222</epage><pages>211-222</pages><issn>1877-2641</issn><eissn>1877-265X</eissn><abstract>A specific macroalgal biomass decomposing fungus SL1 newly isolated and identified as
Aspergillus fumigatus
was found to be an efficient cellulases producer. This strain when cultivated on the whole green macroalgae as sole carbon source permitted the production of specific cellulases (specific activities of 30 and 33 U/mg of proteins for endoglucanase and β-glucosidase, respectively) different from commercial ones as shown by zymography. The application of the produced cellulases-based enzymatic cocktail for the saccharification of alkali pretreated
Ulva
sp. biomass yielded of 58%. This saccharification rate was optimized using response surface methodology (RSM). An increase of 36% in saccharification yield was obtained under optimized conditions (13 U CMCase, 4% substrate and 135 rpm agitation) which agreed with model predictions. The biocatalytic conversion using specific fungal cellulases may be a promising approach for the biodegradation of stranded macroalgae and its valorization mostly for bioethanol production.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s12649-018-0397-4</doi><tpages>12</tpages></addata></record> |
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| subjects | Algae Biodegradation Biofuels Biomass Carbon sources Cellobiase Cellulase Conversion Endoglucanase Engineering Environment Environmental Engineering/Biotechnology Ethanol Fungi Glucosidase Industrial Pollution Prevention Original Paper Renewable and Green Energy Response surface methodology Saccharification Substrates Waste Management/Waste Technology β-Glucosidase |
| title | Efficient Biocatalytic Conversion of Stranded Green Macroalgal Biomass Using a Specific Cellulases-Based Cocktail |
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