Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus
— The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The resu...
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| Veröffentlicht in: | Russian journal of bioorganic chemistry 2019-11, Vol.45 (6), p.774-782 |
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| container_title | Russian journal of bioorganic chemistry |
| container_volume | 45 |
| creator | Markov, A. V. Kupryushkin, M. S. Goncharova, E. P. Amirkhanov, R. N. Vasilyeva, S. V. Pyshnyi, D. V. Zenkova, M. A. Logashenko, E. B. |
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The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The results of screening have shown that PB1-2AUG-R aON targeted towards the AUG codon region of the segment 2 of virus genome possessed the highest antiviral activity. The synthesized morpholino analog (PMO) and the new phosphoryl guanidine oligodeoxyribonucleotide (PGO) with the sequence of oligonucleotide PB1-2AUG-R provided a comparable biological effects: the influenza virus titer in MDCK cell culture was reduced by 15 times compared to the control when PGO was used in the concentration of 10 µM and by 40 times when PMO was used in the concentration of 20 µM. For the delivery of electrically neutral analogs of oligonucleotides (PGO and PMO), the perforation method proposed for PMO was used. |
| doi_str_mv | 10.1134/S1068162019060268 |
| format | Article |
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The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The results of screening have shown that PB1-2AUG-R aON targeted towards the AUG codon region of the segment 2 of virus genome possessed the highest antiviral activity. The synthesized morpholino analog (PMO) and the new phosphoryl guanidine oligodeoxyribonucleotide (PGO) with the sequence of oligonucleotide PB1-2AUG-R provided a comparable biological effects: the influenza virus titer in MDCK cell culture was reduced by 15 times compared to the control when PGO was used in the concentration of 10 µM and by 40 times when PMO was used in the concentration of 20 µM. For the delivery of electrically neutral analogs of oligonucleotides (PGO and PMO), the perforation method proposed for PMO was used.</description><identifier>ISSN: 1068-1620</identifier><identifier>EISSN: 1608-330X</identifier><identifier>DOI: 10.1134/S1068162019060268</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Biochemistry ; Biological effects ; Biomedical and Life Sciences ; Biomedicine ; Bioorganic Chemistry ; Genomes ; Influenza ; Life Sciences ; Oligonucleotides ; Organic Chemistry ; Screening ; Viruses</subject><ispartof>Russian journal of bioorganic chemistry, 2019-11, Vol.45 (6), p.774-782</ispartof><rights>Pleiades Publishing, Ltd. 2019</rights><rights>Pleiades Publishing, Ltd. 2019.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3228-7e4c95a3495671d089ef114207cb027bf8816db6a3bdcdeb2ee7e525de8ae33</citedby><cites>FETCH-LOGICAL-c3228-7e4c95a3495671d089ef114207cb027bf8816db6a3bdcdeb2ee7e525de8ae33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S1068162019060268$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S1068162019060268$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids></links><search><creatorcontrib>Markov, A. V.</creatorcontrib><creatorcontrib>Kupryushkin, M. S.</creatorcontrib><creatorcontrib>Goncharova, E. P.</creatorcontrib><creatorcontrib>Amirkhanov, R. N.</creatorcontrib><creatorcontrib>Vasilyeva, S. V.</creatorcontrib><creatorcontrib>Pyshnyi, D. V.</creatorcontrib><creatorcontrib>Zenkova, M. A.</creatorcontrib><creatorcontrib>Logashenko, E. B.</creatorcontrib><title>Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus</title><title>Russian journal of bioorganic chemistry</title><addtitle>Russ J Bioorg Chem</addtitle><description>—
The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The results of screening have shown that PB1-2AUG-R aON targeted towards the AUG codon region of the segment 2 of virus genome possessed the highest antiviral activity. The synthesized morpholino analog (PMO) and the new phosphoryl guanidine oligodeoxyribonucleotide (PGO) with the sequence of oligonucleotide PB1-2AUG-R provided a comparable biological effects: the influenza virus titer in MDCK cell culture was reduced by 15 times compared to the control when PGO was used in the concentration of 10 µM and by 40 times when PMO was used in the concentration of 20 µM. For the delivery of electrically neutral analogs of oligonucleotides (PGO and PMO), the perforation method proposed for PMO was used.</description><subject>Biochemistry</subject><subject>Biological effects</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bioorganic Chemistry</subject><subject>Genomes</subject><subject>Influenza</subject><subject>Life Sciences</subject><subject>Oligonucleotides</subject><subject>Organic Chemistry</subject><subject>Screening</subject><subject>Viruses</subject><issn>1068-1620</issn><issn>1608-330X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1UEtOwzAQtRBIlMIB2FliHRjb-TjLquJTqdBFEWIXOfGkuHKdYiegcgqOxpFIVCQWiNW80fuM5hFyzuCSMRFfLRmkkqUcWA4p8FQekBFLQUZCwPNhj3s6GvhjchLCGoABJHJE3MS15s14ZemkGlC7o01NFX3Adzq1KoRhnb7gxlTK2h29b7SpDWo6GAO6gHRhzapxXWWxaY3GQNVKGRdaOnO17dB9KPr1SZ-M78IpOaqVDXj2M8dkeXP9OL2L5ovb2XQyjyrBuYwyjKs8USLOkzRjGmSONWMxh6wqgWdlLftfdZkqUepKY8kRM0x4olEqFGJMLvapW9-8dhjaYt103vUHCy5iwTPgIHsV26sq34TgsS623myU3xUMiqHU4k-pvYfvPaHXuhX63-T_Td9WsXo6</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Markov, A. V.</creator><creator>Kupryushkin, M. S.</creator><creator>Goncharova, E. P.</creator><creator>Amirkhanov, R. N.</creator><creator>Vasilyeva, S. V.</creator><creator>Pyshnyi, D. V.</creator><creator>Zenkova, M. A.</creator><creator>Logashenko, E. B.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20191101</creationdate><title>Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus</title><author>Markov, A. V. ; Kupryushkin, M. S. ; Goncharova, E. P. ; Amirkhanov, R. N. ; Vasilyeva, S. V. ; Pyshnyi, D. V. ; Zenkova, M. A. ; Logashenko, E. B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3228-7e4c95a3495671d089ef114207cb027bf8816db6a3bdcdeb2ee7e525de8ae33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biochemistry</topic><topic>Biological effects</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bioorganic Chemistry</topic><topic>Genomes</topic><topic>Influenza</topic><topic>Life Sciences</topic><topic>Oligonucleotides</topic><topic>Organic Chemistry</topic><topic>Screening</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Markov, A. V.</creatorcontrib><creatorcontrib>Kupryushkin, M. S.</creatorcontrib><creatorcontrib>Goncharova, E. P.</creatorcontrib><creatorcontrib>Amirkhanov, R. N.</creatorcontrib><creatorcontrib>Vasilyeva, S. V.</creatorcontrib><creatorcontrib>Pyshnyi, D. V.</creatorcontrib><creatorcontrib>Zenkova, M. A.</creatorcontrib><creatorcontrib>Logashenko, E. B.</creatorcontrib><collection>CrossRef</collection><jtitle>Russian journal of bioorganic chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Markov, A. V.</au><au>Kupryushkin, M. S.</au><au>Goncharova, E. P.</au><au>Amirkhanov, R. N.</au><au>Vasilyeva, S. V.</au><au>Pyshnyi, D. V.</au><au>Zenkova, M. A.</au><au>Logashenko, E. B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus</atitle><jtitle>Russian journal of bioorganic chemistry</jtitle><stitle>Russ J Bioorg Chem</stitle><date>2019-11-01</date><risdate>2019</risdate><volume>45</volume><issue>6</issue><spage>774</spage><epage>782</epage><pages>774-782</pages><issn>1068-1620</issn><eissn>1608-330X</eissn><abstract>—
The paper reports the synthesis of a series of antisense oligonucleotides (aONs) directed against different segments of the influenza A virus genome (H1N1) and screening of their antiviral activity in the influenza A virus (H1N1)/MDCK cells and influenza A virus (H1N1)/A549 cells systems. The results of screening have shown that PB1-2AUG-R aON targeted towards the AUG codon region of the segment 2 of virus genome possessed the highest antiviral activity. The synthesized morpholino analog (PMO) and the new phosphoryl guanidine oligodeoxyribonucleotide (PGO) with the sequence of oligonucleotide PB1-2AUG-R provided a comparable biological effects: the influenza virus titer in MDCK cell culture was reduced by 15 times compared to the control when PGO was used in the concentration of 10 µM and by 40 times when PMO was used in the concentration of 20 µM. For the delivery of electrically neutral analogs of oligonucleotides (PGO and PMO), the perforation method proposed for PMO was used.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S1068162019060268</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biochemistry Biological effects Biomedical and Life Sciences Biomedicine Bioorganic Chemistry Genomes Influenza Life Sciences Oligonucleotides Organic Chemistry Screening Viruses |
| title | Antiviral Activity of a New Class of Chemically Modified Antisense Oligonucleotides against Influenza А Virus |
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