Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro
In this study, we describe a new in vitro tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used Dermacentor andersoni and Anaplasma marginale as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions...
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| Veröffentlicht in: | Scientific reports 2020-01, Vol.10 (1), p.470-470, Article 470 |
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| creator | Vimonish, Rubikah Johnson, Wendell C. Mousel, Michelle R. Brayton, Kelly A. Scoles, Glen A. Noh, Susan M. Ueti, Massaro W. |
| description | In this study, we describe a new
in vitro
tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used
Dermacentor andersoni
and
Anaplasma marginale
as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable
A. marginale
. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with
A. marginale
. However, salivary gland infection rates decreased as the percentage of parasitized erythrocytes decreased during tick acquisition feeding. Bacteria recovered from the
in vitro
system were able to infect a naïve bovine host. Using the highly transmissible
A. marginale
St. Maries strain, we demonstrated that the artificial tick feeding system is a suitable tool to study tick-pathogen interactions and that
A. marginale
tick salivary gland infection is dose dependent. This work demonstrates the utility of an artificial tick feeding system to directly study the association between the number of acquired pathogens and transmissibility by ticks. |
| doi_str_mv | 10.1038/s41598-019-57390-y |
| format | Article |
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in vitro
tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used
Dermacentor andersoni
and
Anaplasma marginale
as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable
A. marginale
. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with
A. marginale
. However, salivary gland infection rates decreased as the percentage of parasitized erythrocytes decreased during tick acquisition feeding. Bacteria recovered from the
in vitro
system were able to infect a naïve bovine host. Using the highly transmissible
A. marginale
St. Maries strain, we demonstrated that the artificial tick feeding system is a suitable tool to study tick-pathogen interactions and that
A. marginale
tick salivary gland infection is dose dependent. This work demonstrates the utility of an artificial tick feeding system to directly study the association between the number of acquired pathogens and transmissibility by ticks.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/s41598-019-57390-y</identifier><identifier>PMID: 31949241</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>13 ; 13/56 ; 631/326/41 ; 631/326/417 ; Anaplasma marginale ; Anaplasma marginale - physiology ; Anaplasmataceae Infections - microbiology ; Anaplasmataceae Infections - transmission ; Animals ; Arachnid Vectors - physiology ; Arachnids ; Blood ; Cattle ; Cattle Diseases - microbiology ; Cattle Diseases - parasitology ; Cattle Diseases - transmission ; Dermacentor - microbiology ; Dermacentor - physiology ; Dermacentor andersoni ; Digestive System - microbiology ; Digestive System - parasitology ; Erythrocytes ; Feeding ; Feeding Behavior - physiology ; Humanities and Social Sciences ; Infections ; Midgut ; multidisciplinary ; Multidisciplinary Sciences ; Pathogens ; Quantitative analysis ; Salivary gland ; Salivary Glands - microbiology ; Salivary Glands - parasitology ; Science ; Science & Technology ; Science & Technology - Other Topics ; Science (multidisciplinary) ; Tick Infestations - microbiology ; Tick Infestations - parasitology ; Tick Infestations - veterinary</subject><ispartof>Scientific reports, 2020-01, Vol.10 (1), p.470-470, Article 470</ispartof><rights>The Author(s) 2020</rights><rights>This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>true</woscitedreferencessubscribed><woscitedreferencescount>12</woscitedreferencescount><woscitedreferencesoriginalsourcerecordid>wos000551461800021</woscitedreferencesoriginalsourcerecordid><citedby>FETCH-LOGICAL-c540t-229f31fd57deed43e4af3ef680dbe22840b77e553f04ffa9fae9ecd461f47dbf3</citedby><cites>FETCH-LOGICAL-c540t-229f31fd57deed43e4af3ef680dbe22840b77e553f04ffa9fae9ecd461f47dbf3</cites><orcidid>0000-0001-7077-5424</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965182/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC6965182/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,315,728,781,785,865,886,2115,27929,27930,28253,41125,42194,51581,53796,53798</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31949241$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vimonish, Rubikah</creatorcontrib><creatorcontrib>Johnson, Wendell C.</creatorcontrib><creatorcontrib>Mousel, Michelle R.</creatorcontrib><creatorcontrib>Brayton, Kelly A.</creatorcontrib><creatorcontrib>Scoles, Glen A.</creatorcontrib><creatorcontrib>Noh, Susan M.</creatorcontrib><creatorcontrib>Ueti, Massaro W.</creatorcontrib><title>Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>SCI REP-UK</addtitle><addtitle>Sci Rep</addtitle><description>In this study, we describe a new
in vitro
tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used
Dermacentor andersoni
and
Anaplasma marginale
as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable
A. marginale
. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with
A. marginale
. However, salivary gland infection rates decreased as the percentage of parasitized erythrocytes decreased during tick acquisition feeding. Bacteria recovered from the
in vitro
system were able to infect a naïve bovine host. Using the highly transmissible
A. marginale
St. Maries strain, we demonstrated that the artificial tick feeding system is a suitable tool to study tick-pathogen interactions and that
A. marginale
tick salivary gland infection is dose dependent. This work demonstrates the utility of an artificial tick feeding system to directly study the association between the number of acquired pathogens and transmissibility by ticks.</description><subject>13</subject><subject>13/56</subject><subject>631/326/41</subject><subject>631/326/417</subject><subject>Anaplasma marginale</subject><subject>Anaplasma marginale - physiology</subject><subject>Anaplasmataceae Infections - microbiology</subject><subject>Anaplasmataceae Infections - transmission</subject><subject>Animals</subject><subject>Arachnid Vectors - physiology</subject><subject>Arachnids</subject><subject>Blood</subject><subject>Cattle</subject><subject>Cattle Diseases - microbiology</subject><subject>Cattle Diseases - parasitology</subject><subject>Cattle Diseases - transmission</subject><subject>Dermacentor - microbiology</subject><subject>Dermacentor - physiology</subject><subject>Dermacentor andersoni</subject><subject>Digestive System - microbiology</subject><subject>Digestive System - parasitology</subject><subject>Erythrocytes</subject><subject>Feeding</subject><subject>Feeding Behavior - physiology</subject><subject>Humanities and Social Sciences</subject><subject>Infections</subject><subject>Midgut</subject><subject>multidisciplinary</subject><subject>Multidisciplinary Sciences</subject><subject>Pathogens</subject><subject>Quantitative analysis</subject><subject>Salivary gland</subject><subject>Salivary Glands - microbiology</subject><subject>Salivary Glands - parasitology</subject><subject>Science</subject><subject>Science & Technology</subject><subject>Science & Technology - Other Topics</subject><subject>Science (multidisciplinary)</subject><subject>Tick Infestations - microbiology</subject><subject>Tick Infestations - parasitology</subject><subject>Tick Infestations - veterinary</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>C6C</sourceid><sourceid>AOWDO</sourceid><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkUtr3DAUhU1paUKaP9BFMXRTKG70tKVNIUyfEAiBdi1k-2qqYEsTSZ7if195nE7TLEq1kdD9zuWee4riJUbvMKLiIjLMpagQlhVvqETV_KQ4JYjxilBCnj54nxTnMd6ifDiRDMvnxQnFkknC8Gnhbybtkk062T2U2ulhjjaW3pSXTu8GHUddjjpsba7kenc32WiT9S6zfZmCdnG0MS4f7Vx-gDDqDlzyYalDiN7Z0kBfWlfubQr-RfHM6CHC-f19Vnz_9PHb5kt1df356-byquo4Q6kiRBqKTc-bHqBnFJg2FEwtUN8CIYKhtmmAc2oQM0ZLo0FC17MaG9b0raFnxfu1725qR-iXmYIe1C7Y7GZWXlv1d8XZH2rr96qWNceC5AZv7hsEfzdBTCr77GAYtAM_RUUowzXBSDQZff0IvfVTyAs7UIQ2jWAiU2SluuBjDGCOw2CklkjVGqnKkapDpGrOolcPbRwlvwPMgFiBn9B6EzsLroMjtmTOcd6KyC-CN4ecvdv4yaUsffv_0kzTlY6ZcFsIf0z-Y_5fQabRlw</recordid><startdate>20200116</startdate><enddate>20200116</enddate><creator>Vimonish, Rubikah</creator><creator>Johnson, Wendell C.</creator><creator>Mousel, Michelle R.</creator><creator>Brayton, Kelly A.</creator><creator>Scoles, Glen A.</creator><creator>Noh, Susan M.</creator><creator>Ueti, Massaro W.</creator><general>Nature Publishing Group UK</general><general>NATURE PORTFOLIO</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>AOWDO</scope><scope>BLEPL</scope><scope>DTL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><orcidid>https://orcid.org/0000-0001-7077-5424</orcidid></search><sort><creationdate>20200116</creationdate><title>Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro</title><author>Vimonish, Rubikah ; Johnson, Wendell C. ; Mousel, Michelle R. ; Brayton, Kelly A. ; Scoles, Glen A. ; Noh, Susan M. ; Ueti, Massaro W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c540t-229f31fd57deed43e4af3ef680dbe22840b77e553f04ffa9fae9ecd461f47dbf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>13</topic><topic>13/56</topic><topic>631/326/41</topic><topic>631/326/417</topic><topic>Anaplasma marginale</topic><topic>Anaplasma marginale - physiology</topic><topic>Anaplasmataceae Infections - microbiology</topic><topic>Anaplasmataceae Infections - transmission</topic><topic>Animals</topic><topic>Arachnid Vectors - physiology</topic><topic>Arachnids</topic><topic>Blood</topic><topic>Cattle</topic><topic>Cattle Diseases - microbiology</topic><topic>Cattle Diseases - parasitology</topic><topic>Cattle Diseases - transmission</topic><topic>Dermacentor - microbiology</topic><topic>Dermacentor - physiology</topic><topic>Dermacentor andersoni</topic><topic>Digestive System - microbiology</topic><topic>Digestive System - parasitology</topic><topic>Erythrocytes</topic><topic>Feeding</topic><topic>Feeding Behavior - physiology</topic><topic>Humanities and Social Sciences</topic><topic>Infections</topic><topic>Midgut</topic><topic>multidisciplinary</topic><topic>Multidisciplinary Sciences</topic><topic>Pathogens</topic><topic>Quantitative analysis</topic><topic>Salivary gland</topic><topic>Salivary Glands - microbiology</topic><topic>Salivary Glands - parasitology</topic><topic>Science</topic><topic>Science & Technology</topic><topic>Science & Technology - Other Topics</topic><topic>Science (multidisciplinary)</topic><topic>Tick Infestations - microbiology</topic><topic>Tick Infestations - parasitology</topic><topic>Tick Infestations - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vimonish, Rubikah</creatorcontrib><creatorcontrib>Johnson, Wendell C.</creatorcontrib><creatorcontrib>Mousel, Michelle R.</creatorcontrib><creatorcontrib>Brayton, Kelly A.</creatorcontrib><creatorcontrib>Scoles, Glen A.</creatorcontrib><creatorcontrib>Noh, Susan M.</creatorcontrib><creatorcontrib>Ueti, Massaro W.</creatorcontrib><collection>Springer Nature OA/Free Journals</collection><collection>Web of Science - Science Citation Index Expanded - 2020</collection><collection>Web of Science Core Collection</collection><collection>Science Citation Index Expanded</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vimonish, Rubikah</au><au>Johnson, Wendell C.</au><au>Mousel, Michelle R.</au><au>Brayton, Kelly A.</au><au>Scoles, Glen A.</au><au>Noh, Susan M.</au><au>Ueti, Massaro W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><stitle>SCI REP-UK</stitle><addtitle>Sci Rep</addtitle><date>2020-01-16</date><risdate>2020</risdate><volume>10</volume><issue>1</issue><spage>470</spage><epage>470</epage><pages>470-470</pages><artnum>470</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>In this study, we describe a new
in vitro
tick feeding system that facilitates the study of ticks and tick-borne pathogens. To optimize the system, we used
Dermacentor andersoni
and
Anaplasma marginale
as a tick-pathogen interaction model. Ticks were fed on bovine blood containing 10-fold dilutions of the pathogen to determine the effect of dose on tick infection rate. After feeding on infected blood, ticks were transferred to uninfected blood to stimulate bacterial replication within the tick vector. During stimulation feeding, blood samples were collected daily to determine if infected ticks secreted viable
A. marginale
. The results demonstrated similar attachment rates between the first and second tick feeding. Tick midgut and salivary glands were infected with
A. marginale
. However, salivary gland infection rates decreased as the percentage of parasitized erythrocytes decreased during tick acquisition feeding. Bacteria recovered from the
in vitro
system were able to infect a naïve bovine host. Using the highly transmissible
A. marginale
St. Maries strain, we demonstrated that the artificial tick feeding system is a suitable tool to study tick-pathogen interactions and that
A. marginale
tick salivary gland infection is dose dependent. This work demonstrates the utility of an artificial tick feeding system to directly study the association between the number of acquired pathogens and transmissibility by ticks.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>31949241</pmid><doi>10.1038/s41598-019-57390-y</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-7077-5424</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | 13 13/56 631/326/41 631/326/417 Anaplasma marginale Anaplasma marginale - physiology Anaplasmataceae Infections - microbiology Anaplasmataceae Infections - transmission Animals Arachnid Vectors - physiology Arachnids Blood Cattle Cattle Diseases - microbiology Cattle Diseases - parasitology Cattle Diseases - transmission Dermacentor - microbiology Dermacentor - physiology Dermacentor andersoni Digestive System - microbiology Digestive System - parasitology Erythrocytes Feeding Feeding Behavior - physiology Humanities and Social Sciences Infections Midgut multidisciplinary Multidisciplinary Sciences Pathogens Quantitative analysis Salivary gland Salivary Glands - microbiology Salivary Glands - parasitology Science Science & Technology Science & Technology - Other Topics Science (multidisciplinary) Tick Infestations - microbiology Tick Infestations - parasitology Tick Infestations - veterinary |
| title | Quantitative analysis of Anaplasma marginale acquisition and transmission by Dermacentor andersoni fed in vitro |
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