Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy
Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metaboli...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 2020-01, Vol.152 (2), p.252-262 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 262 |
|---|---|
| container_issue | 2 |
| container_start_page | 252 |
| container_title | Journal of neurochemistry |
| container_volume | 152 |
| creator | Lepore, Mario G. Buscemi, Lara Hirt, Lorenz Lei, Hongxia |
| description | Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. We modeled global ischemia with one‐stage 4‐vessel‐occlusion (4VO) in rats. Vascular structures were assessed immediately by magnetic resonance angiography. The neurochemical responses in the bilateral cortex were measured 1 h after stroke onset by 1H‐MRS. Then we used Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles to seek metabolic signatures for ischemic severity subgroups. This approach was further tested on neurochemical profiles of mouse striatum 1 h after permanent middle cerebral artery occlusion, where vascular blood flow was monitored by laser Doppler. Magnetic resonance angiography identified successful 4VO from controls and incomplete global ischemia (e.g., 3VO). 1H‐MR spectra of rat cortex after 4VO showed a specific metabolic pattern, distinct from that of respective controls and rats with 3VO. Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles revealed metabolic signatures of acute ischemia that may be extended to mice after permanent middle cerebral artery occlusion. Fingerprinting severity of acute ischemia using neurochemical information may improve MR diagnosis in stroke patients.
We propose the high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. 1H‐MR spectra of rat cortex after 4‐vessel‐ollcusion (4VO) show a specific metabolic pattern, distinct from that of respective controls and rats with the incomplete occlusion, that is, 3VO. Partial‐Least‐Squares discriminant analysis (PLS‐DA) on the overall neurochemical profiles reveal the metabolic signature of acute ischemia. |
| doi_str_mv | 10.1111/jnc.14922 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_wiley</sourceid><recordid>TN_cdi_proquest_journals_2341925081</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2341925081</sourcerecordid><originalsourceid>FETCH-LOGICAL-p1032-fe0cc2b71f1b07f55bb82c834a280dc4a283f649350ce6726b0cd320837daadb3</originalsourceid><addsrcrecordid>eNotkE1OwzAQhS0EEqWw4AaWWKf1X_6WqAIKKrCBteU4dusqsYOdFGWDOAJn5CS4LbN5o5mn96QPgGuMZjjOfGvlDLOSkBMwwSzHCcNpeQomCBGSUMTIObgIYYsQzliGJ-DrWfWico2RUBu7Vr7zxvYB1iZIb1pjRR_PMKid8qYfodNQyKFXMP43qjUCDmFvMBbuzM7BjVlvfr9_tFFNDfEStmJtVR_TvQrOCisVDJ2SvXdBum68BGdaNEFd_esUvN_fvS2Wyer14XFxu0o6jChJtEJSkirHGlco12laVQWRBWWCFKiWe6E6YyVNkVRZTrIKyZoSVNC8FqKu6BTcHHM77z4GFXq-dYO3sZITynBJUlTg6JofXZ-mUSOPKFrhR44R37PlkS0_sOVPL4vDQv8AZMlx4A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2341925081</pqid></control><display><type>article</type><title>Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>Wiley Online Library (Open Access Collection)</source><source>Free Full-Text Journals in Chemistry</source><creator>Lepore, Mario G. ; Buscemi, Lara ; Hirt, Lorenz ; Lei, Hongxia</creator><creatorcontrib>Lepore, Mario G. ; Buscemi, Lara ; Hirt, Lorenz ; Lei, Hongxia</creatorcontrib><description>Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. We modeled global ischemia with one‐stage 4‐vessel‐occlusion (4VO) in rats. Vascular structures were assessed immediately by magnetic resonance angiography. The neurochemical responses in the bilateral cortex were measured 1 h after stroke onset by 1H‐MRS. Then we used Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles to seek metabolic signatures for ischemic severity subgroups. This approach was further tested on neurochemical profiles of mouse striatum 1 h after permanent middle cerebral artery occlusion, where vascular blood flow was monitored by laser Doppler. Magnetic resonance angiography identified successful 4VO from controls and incomplete global ischemia (e.g., 3VO). 1H‐MR spectra of rat cortex after 4VO showed a specific metabolic pattern, distinct from that of respective controls and rats with 3VO. Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles revealed metabolic signatures of acute ischemia that may be extended to mice after permanent middle cerebral artery occlusion. Fingerprinting severity of acute ischemia using neurochemical information may improve MR diagnosis in stroke patients.
We propose the high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. 1H‐MR spectra of rat cortex after 4‐vessel‐ollcusion (4VO) show a specific metabolic pattern, distinct from that of respective controls and rats with the incomplete occlusion, that is, 3VO. Partial‐Least‐Squares discriminant analysis (PLS‐DA) on the overall neurochemical profiles reveal the metabolic signature of acute ischemia.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/jnc.14922</identifier><language>eng</language><publisher>New York: Blackwell Publishing Ltd</publisher><subject>1H magnetic resonance spectroscopy biomarker ; acute ischemia ; Angiography ; Blood flow ; Blood vessels ; Cerebral blood flow ; cortex ; Discriminant analysis ; Fingerprinting ; Fingerprints ; Imaging techniques ; In vivo methods and tests ; Ischemia ; Magnetic resonance imaging ; Magnetic resonance spectroscopy ; Medical imaging ; Metabolism ; Mice ; Neostriatum ; Neuroimaging ; Occlusion ; Resonance ; rodent ; Spectroscopy ; Spectrum analysis ; striatum ; Stroke ; Subgroups</subject><ispartof>Journal of neurochemistry, 2020-01, Vol.152 (2), p.252-262</ispartof><rights>2019 International Society for Neurochemistry</rights><rights>Copyright © 2020 International Society for Neurochemistry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><orcidid>0000-0002-4065-9331</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjnc.14922$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjnc.14922$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,1435,27931,27932,45581,45582,46416,46840</link.rule.ids></links><search><creatorcontrib>Lepore, Mario G.</creatorcontrib><creatorcontrib>Buscemi, Lara</creatorcontrib><creatorcontrib>Hirt, Lorenz</creatorcontrib><creatorcontrib>Lei, Hongxia</creatorcontrib><title>Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy</title><title>Journal of neurochemistry</title><description>Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. We modeled global ischemia with one‐stage 4‐vessel‐occlusion (4VO) in rats. Vascular structures were assessed immediately by magnetic resonance angiography. The neurochemical responses in the bilateral cortex were measured 1 h after stroke onset by 1H‐MRS. Then we used Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles to seek metabolic signatures for ischemic severity subgroups. This approach was further tested on neurochemical profiles of mouse striatum 1 h after permanent middle cerebral artery occlusion, where vascular blood flow was monitored by laser Doppler. Magnetic resonance angiography identified successful 4VO from controls and incomplete global ischemia (e.g., 3VO). 1H‐MR spectra of rat cortex after 4VO showed a specific metabolic pattern, distinct from that of respective controls and rats with 3VO. Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles revealed metabolic signatures of acute ischemia that may be extended to mice after permanent middle cerebral artery occlusion. Fingerprinting severity of acute ischemia using neurochemical information may improve MR diagnosis in stroke patients.
We propose the high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. 1H‐MR spectra of rat cortex after 4‐vessel‐ollcusion (4VO) show a specific metabolic pattern, distinct from that of respective controls and rats with the incomplete occlusion, that is, 3VO. Partial‐Least‐Squares discriminant analysis (PLS‐DA) on the overall neurochemical profiles reveal the metabolic signature of acute ischemia.</description><subject>1H magnetic resonance spectroscopy biomarker</subject><subject>acute ischemia</subject><subject>Angiography</subject><subject>Blood flow</subject><subject>Blood vessels</subject><subject>Cerebral blood flow</subject><subject>cortex</subject><subject>Discriminant analysis</subject><subject>Fingerprinting</subject><subject>Fingerprints</subject><subject>Imaging techniques</subject><subject>In vivo methods and tests</subject><subject>Ischemia</subject><subject>Magnetic resonance imaging</subject><subject>Magnetic resonance spectroscopy</subject><subject>Medical imaging</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Neostriatum</subject><subject>Neuroimaging</subject><subject>Occlusion</subject><subject>Resonance</subject><subject>rodent</subject><subject>Spectroscopy</subject><subject>Spectrum analysis</subject><subject>striatum</subject><subject>Stroke</subject><subject>Subgroups</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNotkE1OwzAQhS0EEqWw4AaWWKf1X_6WqAIKKrCBteU4dusqsYOdFGWDOAJn5CS4LbN5o5mn96QPgGuMZjjOfGvlDLOSkBMwwSzHCcNpeQomCBGSUMTIObgIYYsQzliGJ-DrWfWico2RUBu7Vr7zxvYB1iZIb1pjRR_PMKid8qYfodNQyKFXMP43qjUCDmFvMBbuzM7BjVlvfr9_tFFNDfEStmJtVR_TvQrOCisVDJ2SvXdBum68BGdaNEFd_esUvN_fvS2Wyer14XFxu0o6jChJtEJSkirHGlco12laVQWRBWWCFKiWe6E6YyVNkVRZTrIKyZoSVNC8FqKu6BTcHHM77z4GFXq-dYO3sZITynBJUlTg6JofXZ-mUSOPKFrhR44R37PlkS0_sOVPL4vDQv8AZMlx4A</recordid><startdate>202001</startdate><enddate>202001</enddate><creator>Lepore, Mario G.</creator><creator>Buscemi, Lara</creator><creator>Hirt, Lorenz</creator><creator>Lei, Hongxia</creator><general>Blackwell Publishing Ltd</general><scope>7QR</scope><scope>7TK</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0002-4065-9331</orcidid></search><sort><creationdate>202001</creationdate><title>Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy</title><author>Lepore, Mario G. ; Buscemi, Lara ; Hirt, Lorenz ; Lei, Hongxia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p1032-fe0cc2b71f1b07f55bb82c834a280dc4a283f649350ce6726b0cd320837daadb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>1H magnetic resonance spectroscopy biomarker</topic><topic>acute ischemia</topic><topic>Angiography</topic><topic>Blood flow</topic><topic>Blood vessels</topic><topic>Cerebral blood flow</topic><topic>cortex</topic><topic>Discriminant analysis</topic><topic>Fingerprinting</topic><topic>Fingerprints</topic><topic>Imaging techniques</topic><topic>In vivo methods and tests</topic><topic>Ischemia</topic><topic>Magnetic resonance imaging</topic><topic>Magnetic resonance spectroscopy</topic><topic>Medical imaging</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Neostriatum</topic><topic>Neuroimaging</topic><topic>Occlusion</topic><topic>Resonance</topic><topic>rodent</topic><topic>Spectroscopy</topic><topic>Spectrum analysis</topic><topic>striatum</topic><topic>Stroke</topic><topic>Subgroups</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lepore, Mario G.</creatorcontrib><creatorcontrib>Buscemi, Lara</creatorcontrib><creatorcontrib>Hirt, Lorenz</creatorcontrib><creatorcontrib>Lei, Hongxia</creatorcontrib><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lepore, Mario G.</au><au>Buscemi, Lara</au><au>Hirt, Lorenz</au><au>Lei, Hongxia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy</atitle><jtitle>Journal of neurochemistry</jtitle><date>2020-01</date><risdate>2020</risdate><volume>152</volume><issue>2</issue><spage>252</spage><epage>262</epage><pages>252-262</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><abstract>Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. We modeled global ischemia with one‐stage 4‐vessel‐occlusion (4VO) in rats. Vascular structures were assessed immediately by magnetic resonance angiography. The neurochemical responses in the bilateral cortex were measured 1 h after stroke onset by 1H‐MRS. Then we used Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles to seek metabolic signatures for ischemic severity subgroups. This approach was further tested on neurochemical profiles of mouse striatum 1 h after permanent middle cerebral artery occlusion, where vascular blood flow was monitored by laser Doppler. Magnetic resonance angiography identified successful 4VO from controls and incomplete global ischemia (e.g., 3VO). 1H‐MR spectra of rat cortex after 4VO showed a specific metabolic pattern, distinct from that of respective controls and rats with 3VO. Partial‐Least‐Squares discriminant analysis on the overall neurochemical profiles revealed metabolic signatures of acute ischemia that may be extended to mice after permanent middle cerebral artery occlusion. Fingerprinting severity of acute ischemia using neurochemical information may improve MR diagnosis in stroke patients.
We propose the high‐field 1H MR Spectroscopy (1H‐MRS) neurochemical profile as a metabolic signature for acute ischemia severity in rodent brains. 1H‐MR spectra of rat cortex after 4‐vessel‐ollcusion (4VO) show a specific metabolic pattern, distinct from that of respective controls and rats with the incomplete occlusion, that is, 3VO. Partial‐Least‐Squares discriminant analysis (PLS‐DA) on the overall neurochemical profiles reveal the metabolic signature of acute ischemia.</abstract><cop>New York</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/jnc.14922</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4065-9331</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 2020-01, Vol.152 (2), p.252-262 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_proquest_journals_2341925081 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; Wiley Online Library (Open Access Collection); Free Full-Text Journals in Chemistry |
| subjects | 1H magnetic resonance spectroscopy biomarker acute ischemia Angiography Blood flow Blood vessels Cerebral blood flow cortex Discriminant analysis Fingerprinting Fingerprints Imaging techniques In vivo methods and tests Ischemia Magnetic resonance imaging Magnetic resonance spectroscopy Medical imaging Metabolism Mice Neostriatum Neuroimaging Occlusion Resonance rodent Spectroscopy Spectrum analysis striatum Stroke Subgroups |
| title | Metabolic fingerprints discriminating severity of acute ischemia using in vivo high‐field 1H magnetic resonance spectroscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-04T09%3A15%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wiley&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Metabolic%20fingerprints%20discriminating%20severity%20of%20acute%20ischemia%20using%20in%20vivo%20high%E2%80%90field%201H%20magnetic%20resonance%20spectroscopy&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Lepore,%20Mario%20G.&rft.date=2020-01&rft.volume=152&rft.issue=2&rft.spage=252&rft.epage=262&rft.pages=252-262&rft.issn=0022-3042&rft.eissn=1471-4159&rft_id=info:doi/10.1111/jnc.14922&rft_dat=%3Cproquest_wiley%3E2341925081%3C/proquest_wiley%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2341925081&rft_id=info:pmid/&rfr_iscdi=true |