Study on sonodynamic effects of N2O-microbubbles

Study on sonodynamic effects of N2O-microbubbles

Objective 　To investigate the sonosensitivity of N2O-microbubbles (N2O-mbs) and its sonodynamic effect on tumor cells. Methods 　The N2O-mbs were prepared by improved mechanical oscillation method. The basic characteristics of N2O-mbs were determined. The cytotoxicity of these microbubbles was evalua...

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Veröffentlicht in:Jie fang jun yi xue za zhi 2019-01, Vol.44 (12), p.1000
Hauptverfasser: Xiao-wen, ZHONG, HUANG, Yan, Lin, DAN, WANG, Zhi-gang, Ze-dan TIAN
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Flow cytometry
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container_issue 12
container_start_page 1000
container_title Jie fang jun yi xue za zhi
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creator Xiao-wen, ZHONG
HUANG, Yan
Lin, DAN
WANG, Zhi-gang
Ze-dan TIAN
description Objective 　To investigate the sonosensitivity of N2O-microbubbles (N2O-mbs) and its sonodynamic effect on tumor cells. Methods 　The N2O-mbs were prepared by improved mechanical oscillation method. The basic characteristics of N2O-mbs were determined. The cytotoxicity of these microbubbles was evaluated by CCK-8 assay. The delivery efficiency of N2O-mbs to MDA-MB-231 cells was observed under a laser scanning confocal microscope. The sonosensitivity of N2O-mbs was evaluated by singlet oxygen sensor green (SOSG) assay and reactive oxygen species assay kit, which detected its extracellular and intracellular reactive oxygen species generation. In vitro sonodynamic properties of N2O-mbs on tumor cells were evaluated by CCK-8 assay, flow cytometry and CAM/PI double staining. Results 　These N2O-mbs presented with homogenized size distribution and milky white suspensions after water reconstitution. The average diameter of N2O-mbs was (458.10±17.26) nm and its zeta potential was (–16.20±0.35) mV. N2O-mbs exhibited a go
doi_str_mv 10.11855/j.issn.0577-7402.2019.12.02
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