CGRP protects the intestinal barrier by reducing intestinal apoptosis in sepsis rats
Objective To investigate the mechanism of calcitonin gene-related peptide (CGRP) in protecting intestinal barrier integrity by reducing intestinal apoptosis in sepsis rats. Methods Thirty SD male rats were randomized into 5 groups (n=6): Normal group, sham group, sepsis group (CLP group), CGRP tre...
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| Veröffentlicht in: | Jie fang jun yi xue za zhi 2019-01, Vol.44 (12), p.1024 |
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| creator | GUO, Jiu-bing Jun-sheng, WANG Jin-zhou, LIN Jia-wei, SUN Dao-jian, ZHANG Wen-jun, XU Ya-yang, CHEN Zhi-peng, XU FENG, Wei SU, Lei Hui-heng, LIU |
| description | Objective To investigate the mechanism of calcitonin gene-related peptide (CGRP) in protecting intestinal barrier integrity by reducing intestinal apoptosis in sepsis rats. Methods Thirty SD male rats were randomized into 5 groups (n=6): Normal group, sham group, sepsis group (CLP group), CGRP treatment group (CGRP group) and anti-CGRP group. Rats were injected with 0.5 ml CGRP (30 μg/kg) in the CGRP group and injected with 0.5 ml CGRP (30 μg/kg) and 0.5 ml CGRP8-37 (30 μg/kg) in the anti- CGRP group. Serum diamine oxidase (DAO) and D-lactic acid levels were measured 30 minutes before the operation and 48 hours after the modeling. Histopathological changes of the small intestine were observed by HE staining; apoptosis of small intestine was detected by TUNEL assay; the expression of phosphorylated Bcl-xl/Bcl-2 related death promoter (p-BAD), phosphorylated extracellular signal-regulated kinase (p-ERK), caspase-3 were observed by immunohistochemistry. Results Compared with the normal group, the small intest |
| doi_str_mv | 10.11855/j.issn.0577-7402.2019.12.06 |
| format | Article |
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Methods Thirty SD male rats were randomized into 5 groups (n=6): Normal group, sham group, sepsis group (CLP group), CGRP treatment group (CGRP group) and anti-CGRP group. Rats were injected with 0.5 ml CGRP (30 μg/kg) in the CGRP group and injected with 0.5 ml CGRP (30 μg/kg) and 0.5 ml CGRP8-37 (30 μg/kg) in the anti- CGRP group. Serum diamine oxidase (DAO) and D-lactic acid levels were measured 30 minutes before the operation and 48 hours after the modeling. Histopathological changes of the small intestine were observed by HE staining; apoptosis of small intestine was detected by TUNEL assay; the expression of phosphorylated Bcl-xl/Bcl-2 related death promoter (p-BAD), phosphorylated extracellular signal-regulated kinase (p-ERK), caspase-3 were observed by immunohistochemistry. Results Compared with the normal group, the small intest</description><identifier>ISSN: 0577-7402</identifier><identifier>DOI: 10.11855/j.issn.0577-7402.2019.12.06</identifier><language>chi</language><publisher>Beijing: People's Military Medical Press</publisher><subject>Acids ; Apoptosis ; Sepsis ; Small intestine</subject><ispartof>Jie fang jun yi xue za zhi, 2019-01, Vol.44 (12), p.1024</ispartof><rights>2019] This work is licensed under [CC BY 3.0 Unported - https://creativecommons.org/licenses/by/3.0/ (“the License”). Notwithstanding the ProQuest Terms and conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>GUO, Jiu-bing</creatorcontrib><creatorcontrib>Jun-sheng, WANG</creatorcontrib><creatorcontrib>Jin-zhou, LIN</creatorcontrib><creatorcontrib>Jia-wei, SUN</creatorcontrib><creatorcontrib>Dao-jian, ZHANG</creatorcontrib><creatorcontrib>Wen-jun, XU</creatorcontrib><creatorcontrib>Ya-yang, CHEN</creatorcontrib><creatorcontrib>Zhi-peng, XU</creatorcontrib><creatorcontrib>FENG, Wei</creatorcontrib><creatorcontrib>SU, Lei</creatorcontrib><creatorcontrib>Hui-heng, LIU</creatorcontrib><title>CGRP protects the intestinal barrier by reducing intestinal apoptosis in sepsis rats</title><title>Jie fang jun yi xue za zhi</title><description>Objective To investigate the mechanism of calcitonin gene-related peptide (CGRP) in protecting intestinal barrier integrity by reducing intestinal apoptosis in sepsis rats. Methods Thirty SD male rats were randomized into 5 groups (n=6): Normal group, sham group, sepsis group (CLP group), CGRP treatment group (CGRP group) and anti-CGRP group. Rats were injected with 0.5 ml CGRP (30 μg/kg) in the CGRP group and injected with 0.5 ml CGRP (30 μg/kg) and 0.5 ml CGRP8-37 (30 μg/kg) in the anti- CGRP group. Serum diamine oxidase (DAO) and D-lactic acid levels were measured 30 minutes before the operation and 48 hours after the modeling. Histopathological changes of the small intestine were observed by HE staining; apoptosis of small intestine was detected by TUNEL assay; the expression of phosphorylated Bcl-xl/Bcl-2 related death promoter (p-BAD), phosphorylated extracellular signal-regulated kinase (p-ERK), caspase-3 were observed by immunohistochemistry. Results Compared with the normal group, the small intest</description><subject>Acids</subject><subject>Apoptosis</subject><subject>Sepsis</subject><subject>Small intestine</subject><issn>0577-7402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNTbsOgjAU7aCJRPmHJrpS20J5zMTHaAw7KVi1hLTYWwb_XkyMcXQ6J-eJ0IZRwlguxLYjGsAQKrIsyhLKCaesIIwTms5Q8JUXKATQDaWM0SRhaYCq8nA-4cFZr1oP2N8V1sYr8NrIHjfSOa0cbp7YqcvYanP7teVgB29BwyRiUMObOelhheZX2YMKP7hE6_2uKo_R9PMYp3bd2dFNC1DzOE65yHNRxP-lXjb_SZU</recordid><startdate>20190101</startdate><enddate>20190101</enddate><creator>GUO, Jiu-bing</creator><creator>Jun-sheng, WANG</creator><creator>Jin-zhou, LIN</creator><creator>Jia-wei, SUN</creator><creator>Dao-jian, ZHANG</creator><creator>Wen-jun, XU</creator><creator>Ya-yang, CHEN</creator><creator>Zhi-peng, XU</creator><creator>FENG, Wei</creator><creator>SU, Lei</creator><creator>Hui-heng, LIU</creator><general>People's Military Medical Press</general><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BVBZV</scope><scope>CCPQU</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope></search><sort><creationdate>20190101</creationdate><title>CGRP protects the intestinal barrier by reducing intestinal apoptosis in sepsis rats</title><author>GUO, Jiu-bing ; Jun-sheng, WANG ; Jin-zhou, LIN ; Jia-wei, SUN ; Dao-jian, ZHANG ; Wen-jun, XU ; Ya-yang, CHEN ; Zhi-peng, XU ; FENG, Wei ; SU, Lei ; Hui-heng, LIU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-proquest_journals_23362588593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2019</creationdate><topic>Acids</topic><topic>Apoptosis</topic><topic>Sepsis</topic><topic>Small intestine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>GUO, Jiu-bing</creatorcontrib><creatorcontrib>Jun-sheng, WANG</creatorcontrib><creatorcontrib>Jin-zhou, LIN</creatorcontrib><creatorcontrib>Jia-wei, SUN</creatorcontrib><creatorcontrib>Dao-jian, ZHANG</creatorcontrib><creatorcontrib>Wen-jun, XU</creatorcontrib><creatorcontrib>Ya-yang, CHEN</creatorcontrib><creatorcontrib>Zhi-peng, XU</creatorcontrib><creatorcontrib>FENG, Wei</creatorcontrib><creatorcontrib>SU, Lei</creatorcontrib><creatorcontrib>Hui-heng, LIU</creatorcontrib><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>East & South Asia Database</collection><collection>ProQuest One Community College</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><jtitle>Jie fang jun yi xue za zhi</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>GUO, Jiu-bing</au><au>Jun-sheng, WANG</au><au>Jin-zhou, LIN</au><au>Jia-wei, SUN</au><au>Dao-jian, ZHANG</au><au>Wen-jun, XU</au><au>Ya-yang, CHEN</au><au>Zhi-peng, XU</au><au>FENG, Wei</au><au>SU, Lei</au><au>Hui-heng, LIU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CGRP protects the intestinal barrier by reducing intestinal apoptosis in sepsis rats</atitle><jtitle>Jie fang jun yi xue za zhi</jtitle><date>2019-01-01</date><risdate>2019</risdate><volume>44</volume><issue>12</issue><spage>1024</spage><pages>1024-</pages><issn>0577-7402</issn><abstract>Objective To investigate the mechanism of calcitonin gene-related peptide (CGRP) in protecting intestinal barrier integrity by reducing intestinal apoptosis in sepsis rats. Methods Thirty SD male rats were randomized into 5 groups (n=6): Normal group, sham group, sepsis group (CLP group), CGRP treatment group (CGRP group) and anti-CGRP group. Rats were injected with 0.5 ml CGRP (30 μg/kg) in the CGRP group and injected with 0.5 ml CGRP (30 μg/kg) and 0.5 ml CGRP8-37 (30 μg/kg) in the anti- CGRP group. Serum diamine oxidase (DAO) and D-lactic acid levels were measured 30 minutes before the operation and 48 hours after the modeling. Histopathological changes of the small intestine were observed by HE staining; apoptosis of small intestine was detected by TUNEL assay; the expression of phosphorylated Bcl-xl/Bcl-2 related death promoter (p-BAD), phosphorylated extracellular signal-regulated kinase (p-ERK), caspase-3 were observed by immunohistochemistry. Results Compared with the normal group, the small intest</abstract><cop>Beijing</cop><pub>People's Military Medical Press</pub><doi>10.11855/j.issn.0577-7402.2019.12.06</doi></addata></record> |
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| subjects | Acids Apoptosis Sepsis Small intestine |
| title | CGRP protects the intestinal barrier by reducing intestinal apoptosis in sepsis rats |
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