One hour enzymatic synthesis of structure lipids enriched unsaturated fatty acids from silkworm pupae oil under microwave irradiation
BACKGROUND The silkworm pupae of Bombyx mori L. are considered to be a valuable unsaturated fatty acids (UFAs) resource. Enzymatic transesterification, which can produce structured lipids (SLs), is regarded as a desirable approach to utilize this resource, but requires long reaction time. A method t...
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| Veröffentlicht in: | Journal of chemical technology and biotechnology (1986) 2020-02, Vol.95 (2), p.363-372 |
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| container_title | Journal of chemical technology and biotechnology (1986) |
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| creator | Wang, Jin‐Zheng Liu, Xi Li, Wen‐Jing Song, Wen‐Miao Herman, Richard Ansah Sheng, Sheng Wu, Fu‐An Wang, Jun |
| description | BACKGROUND
The silkworm pupae of Bombyx mori L. are considered to be a valuable unsaturated fatty acids (UFAs) resource. Enzymatic transesterification, which can produce structured lipids (SLs), is regarded as a desirable approach to utilize this resource, but requires long reaction time. A method to reduce the reaction time and obtain novel SLs is thus desirable. Hence, microwave irradiation was employed to reduce the reaction time and obtain novel SLs. In addition, high‐performance liquid chromatography with an evaporative light scattering detector was employed to investigate the contribution of the fatty acids (FAs) after microwave irradiation.
RESULTS
One hour was required for a 1:5 substrate ratio of silkworm pupae oil (SPO):oleic acid (OA) using a solvent‐free system catalysed by Lipozyme RM IM (7%) at 60 °C with microwave irradiation at 50 W to obtain the novel SLs. The relative content of polyunsaturated FAs glyceride increased from 51.1% to 81.0%, and the relative content of glycerol trioleate (OOO) in SPO obtained in the microwave reactor was twice as high as that in the shaker reactor. In addition, the relative content of OA was increased from 3.9% to 35.8% in total compared with the products from the shaker reactor. Furthermore, the changes of α‐helix relative content of Lipozyme RM IM were observed after the reaction under the microwave irradiation.
CONCLUSION
The results indicated that variations in the FA distribution of enzymatic transesterification reactions caused by microwave irradiation could aid the addition of OA and enrichment of UFAs. © 2019 Society of Chemical Industry |
| doi_str_mv | 10.1002/jctb.5924 |
| format | Article |
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The silkworm pupae of Bombyx mori L. are considered to be a valuable unsaturated fatty acids (UFAs) resource. Enzymatic transesterification, which can produce structured lipids (SLs), is regarded as a desirable approach to utilize this resource, but requires long reaction time. A method to reduce the reaction time and obtain novel SLs is thus desirable. Hence, microwave irradiation was employed to reduce the reaction time and obtain novel SLs. In addition, high‐performance liquid chromatography with an evaporative light scattering detector was employed to investigate the contribution of the fatty acids (FAs) after microwave irradiation.
RESULTS
One hour was required for a 1:5 substrate ratio of silkworm pupae oil (SPO):oleic acid (OA) using a solvent‐free system catalysed by Lipozyme RM IM (7%) at 60 °C with microwave irradiation at 50 W to obtain the novel SLs. The relative content of polyunsaturated FAs glyceride increased from 51.1% to 81.0%, and the relative content of glycerol trioleate (OOO) in SPO obtained in the microwave reactor was twice as high as that in the shaker reactor. In addition, the relative content of OA was increased from 3.9% to 35.8% in total compared with the products from the shaker reactor. Furthermore, the changes of α‐helix relative content of Lipozyme RM IM were observed after the reaction under the microwave irradiation.
CONCLUSION
The results indicated that variations in the FA distribution of enzymatic transesterification reactions caused by microwave irradiation could aid the addition of OA and enrichment of UFAs. © 2019 Society of Chemical Industry</description><identifier>ISSN: 0268-2575</identifier><identifier>EISSN: 1097-4660</identifier><identifier>DOI: 10.1002/jctb.5924</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>biocatalysis ; Bombyx mori ; Enzymatic synthesis ; Fatty acids ; Glycerol ; HPLC‐ELSD ; Irradiation ; Light scattering ; Lipids ; Liquid chromatography ; microwave irradiation ; Oleic acid ; Organic chemistry ; Pupae ; Reaction time ; Reactors ; silkworm pupae oil ; Silkworms ; structure lipids ; Substrates ; Transesterification ; unsaturated fatty acids</subject><ispartof>Journal of chemical technology and biotechnology (1986), 2020-02, Vol.95 (2), p.363-372</ispartof><rights>2019 Society of Chemical Industry</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3344-6c2daade244ecd3a56449c460b434ea80d920066999f1f437ac222d3323ee0ed3</citedby><cites>FETCH-LOGICAL-c3344-6c2daade244ecd3a56449c460b434ea80d920066999f1f437ac222d3323ee0ed3</cites><orcidid>0000-0003-2569-1461</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjctb.5924$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjctb.5924$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids></links><search><creatorcontrib>Wang, Jin‐Zheng</creatorcontrib><creatorcontrib>Liu, Xi</creatorcontrib><creatorcontrib>Li, Wen‐Jing</creatorcontrib><creatorcontrib>Song, Wen‐Miao</creatorcontrib><creatorcontrib>Herman, Richard Ansah</creatorcontrib><creatorcontrib>Sheng, Sheng</creatorcontrib><creatorcontrib>Wu, Fu‐An</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><title>One hour enzymatic synthesis of structure lipids enriched unsaturated fatty acids from silkworm pupae oil under microwave irradiation</title><title>Journal of chemical technology and biotechnology (1986)</title><description>BACKGROUND
The silkworm pupae of Bombyx mori L. are considered to be a valuable unsaturated fatty acids (UFAs) resource. Enzymatic transesterification, which can produce structured lipids (SLs), is regarded as a desirable approach to utilize this resource, but requires long reaction time. A method to reduce the reaction time and obtain novel SLs is thus desirable. Hence, microwave irradiation was employed to reduce the reaction time and obtain novel SLs. In addition, high‐performance liquid chromatography with an evaporative light scattering detector was employed to investigate the contribution of the fatty acids (FAs) after microwave irradiation.
RESULTS
One hour was required for a 1:5 substrate ratio of silkworm pupae oil (SPO):oleic acid (OA) using a solvent‐free system catalysed by Lipozyme RM IM (7%) at 60 °C with microwave irradiation at 50 W to obtain the novel SLs. The relative content of polyunsaturated FAs glyceride increased from 51.1% to 81.0%, and the relative content of glycerol trioleate (OOO) in SPO obtained in the microwave reactor was twice as high as that in the shaker reactor. In addition, the relative content of OA was increased from 3.9% to 35.8% in total compared with the products from the shaker reactor. Furthermore, the changes of α‐helix relative content of Lipozyme RM IM were observed after the reaction under the microwave irradiation.
CONCLUSION
The results indicated that variations in the FA distribution of enzymatic transesterification reactions caused by microwave irradiation could aid the addition of OA and enrichment of UFAs. © 2019 Society of Chemical Industry</description><subject>biocatalysis</subject><subject>Bombyx mori</subject><subject>Enzymatic synthesis</subject><subject>Fatty acids</subject><subject>Glycerol</subject><subject>HPLC‐ELSD</subject><subject>Irradiation</subject><subject>Light scattering</subject><subject>Lipids</subject><subject>Liquid chromatography</subject><subject>microwave irradiation</subject><subject>Oleic acid</subject><subject>Organic chemistry</subject><subject>Pupae</subject><subject>Reaction time</subject><subject>Reactors</subject><subject>silkworm pupae oil</subject><subject>Silkworms</subject><subject>structure lipids</subject><subject>Substrates</subject><subject>Transesterification</subject><subject>unsaturated fatty acids</subject><issn>0268-2575</issn><issn>1097-4660</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp1kD1PwzAQhi0EEqUw8A8sMTGkdWzHaUao-FSlLmW2XPuiuuQL26EKO_8bh7Iy3Un33L33vghdp2SWEkLnex22s6yg_ARNUlLkCReCnKIJoWKR0CzPztGF93tCiFhQMUHf6wbwru0dhuZrqFWwGvuhCTvw1uO2xD64XofeAa5sZ42PnLN6Bwb3jVdxoELsSxXCgJUegdK1Nfa2ej-0rsZd3ynAra0ib8Dh2mrXHtQnYOucMjYqts0lOitV5eHqr07R2-PDZvmcrNZPL8u7VaIZ4zwRmhqlDFDOQRumMsF5obkgW844qAUxBY3GRFEUZVpylitNKTWMUQZAwLApujne7Vz70YMPch-tN1FSUsZYvsgKwSJ1e6Tip947KGXnbK3cIFMix5TlmLIcU47s_MgebAXD_6B8XW7ufzd-AG7igmo</recordid><startdate>202002</startdate><enddate>202002</enddate><creator>Wang, Jin‐Zheng</creator><creator>Liu, Xi</creator><creator>Li, Wen‐Jing</creator><creator>Song, Wen‐Miao</creator><creator>Herman, Richard Ansah</creator><creator>Sheng, Sheng</creator><creator>Wu, Fu‐An</creator><creator>Wang, Jun</creator><general>John Wiley & Sons, Ltd</general><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><orcidid>https://orcid.org/0000-0003-2569-1461</orcidid></search><sort><creationdate>202002</creationdate><title>One hour enzymatic synthesis of structure lipids enriched unsaturated fatty acids from silkworm pupae oil under microwave irradiation</title><author>Wang, Jin‐Zheng ; Liu, Xi ; Li, Wen‐Jing ; Song, Wen‐Miao ; Herman, Richard Ansah ; Sheng, Sheng ; Wu, Fu‐An ; Wang, Jun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3344-6c2daade244ecd3a56449c460b434ea80d920066999f1f437ac222d3323ee0ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>biocatalysis</topic><topic>Bombyx mori</topic><topic>Enzymatic synthesis</topic><topic>Fatty acids</topic><topic>Glycerol</topic><topic>HPLC‐ELSD</topic><topic>Irradiation</topic><topic>Light scattering</topic><topic>Lipids</topic><topic>Liquid chromatography</topic><topic>microwave irradiation</topic><topic>Oleic acid</topic><topic>Organic chemistry</topic><topic>Pupae</topic><topic>Reaction time</topic><topic>Reactors</topic><topic>silkworm pupae oil</topic><topic>Silkworms</topic><topic>structure lipids</topic><topic>Substrates</topic><topic>Transesterification</topic><topic>unsaturated fatty acids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Jin‐Zheng</creatorcontrib><creatorcontrib>Liu, Xi</creatorcontrib><creatorcontrib>Li, Wen‐Jing</creatorcontrib><creatorcontrib>Song, Wen‐Miao</creatorcontrib><creatorcontrib>Herman, Richard Ansah</creatorcontrib><creatorcontrib>Sheng, Sheng</creatorcontrib><creatorcontrib>Wu, Fu‐An</creatorcontrib><creatorcontrib>Wang, Jun</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Jin‐Zheng</au><au>Liu, Xi</au><au>Li, Wen‐Jing</au><au>Song, Wen‐Miao</au><au>Herman, Richard Ansah</au><au>Sheng, Sheng</au><au>Wu, Fu‐An</au><au>Wang, Jun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>One hour enzymatic synthesis of structure lipids enriched unsaturated fatty acids from silkworm pupae oil under microwave irradiation</atitle><jtitle>Journal of chemical technology and biotechnology (1986)</jtitle><date>2020-02</date><risdate>2020</risdate><volume>95</volume><issue>2</issue><spage>363</spage><epage>372</epage><pages>363-372</pages><issn>0268-2575</issn><eissn>1097-4660</eissn><abstract>BACKGROUND
The silkworm pupae of Bombyx mori L. are considered to be a valuable unsaturated fatty acids (UFAs) resource. Enzymatic transesterification, which can produce structured lipids (SLs), is regarded as a desirable approach to utilize this resource, but requires long reaction time. A method to reduce the reaction time and obtain novel SLs is thus desirable. Hence, microwave irradiation was employed to reduce the reaction time and obtain novel SLs. In addition, high‐performance liquid chromatography with an evaporative light scattering detector was employed to investigate the contribution of the fatty acids (FAs) after microwave irradiation.
RESULTS
One hour was required for a 1:5 substrate ratio of silkworm pupae oil (SPO):oleic acid (OA) using a solvent‐free system catalysed by Lipozyme RM IM (7%) at 60 °C with microwave irradiation at 50 W to obtain the novel SLs. The relative content of polyunsaturated FAs glyceride increased from 51.1% to 81.0%, and the relative content of glycerol trioleate (OOO) in SPO obtained in the microwave reactor was twice as high as that in the shaker reactor. In addition, the relative content of OA was increased from 3.9% to 35.8% in total compared with the products from the shaker reactor. Furthermore, the changes of α‐helix relative content of Lipozyme RM IM were observed after the reaction under the microwave irradiation.
CONCLUSION
The results indicated that variations in the FA distribution of enzymatic transesterification reactions caused by microwave irradiation could aid the addition of OA and enrichment of UFAs. © 2019 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><doi>10.1002/jctb.5924</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2569-1461</orcidid></addata></record> |
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| ispartof | Journal of chemical technology and biotechnology (1986), 2020-02, Vol.95 (2), p.363-372 |
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| subjects | biocatalysis Bombyx mori Enzymatic synthesis Fatty acids Glycerol HPLC‐ELSD Irradiation Light scattering Lipids Liquid chromatography microwave irradiation Oleic acid Organic chemistry Pupae Reaction time Reactors silkworm pupae oil Silkworms structure lipids Substrates Transesterification unsaturated fatty acids |
| title | One hour enzymatic synthesis of structure lipids enriched unsaturated fatty acids from silkworm pupae oil under microwave irradiation |
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