Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina
Abstract H2O2 production by certain Lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. This paper describes the kinetics of H2O2 production by two different strains of Lactobacillus paracasei subsp. paracasei under different culture conditions and the effect...
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| Veröffentlicht in: | FEMS immunology and medical microbiology 1999-02, Vol.23 (2), p.87-92 |
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| container_title | FEMS immunology and medical microbiology |
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| creator | Ocaña, Virginia S. Pesce de Ruiz Holgado, Aída A. Nader-Macías, María Elena |
| description | Abstract
H2O2 production by certain Lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. This paper describes the kinetics of H2O2 production by two different strains of Lactobacillus paracasei subsp. paracasei under different culture conditions and the effect of this metabolite on the growth of Staphylococcus aureus. L. paracasei F2 produced 2.72 mmol l−1 H2O2 while L. paracasei F28 produced 1.84 mmol l−1, both in agitated cultures. Although L. paracasei F2 produced a higher H2O2 concentration than L. paracasei F28, H2O2 production per number of live bacterial cells was 10-fold higher for F28. The latter also showed a faster decrease in viability during the stationary phase. There were no detectable levels of H2O2 in cultures without agitation. H2O2-producing lactobacilli inhibited growth of S. aureus in a plaque assay and in mixed cultures, depending on the initial inoculum of the pathogen. |
| doi_str_mv | 10.1111/j.1574-695X.1999.tb01227.x |
| format | Article |
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H2O2 production by certain Lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. This paper describes the kinetics of H2O2 production by two different strains of Lactobacillus paracasei subsp. paracasei under different culture conditions and the effect of this metabolite on the growth of Staphylococcus aureus. L. paracasei F2 produced 2.72 mmol l−1 H2O2 while L. paracasei F28 produced 1.84 mmol l−1, both in agitated cultures. Although L. paracasei F2 produced a higher H2O2 concentration than L. paracasei F28, H2O2 production per number of live bacterial cells was 10-fold higher for F28. The latter also showed a faster decrease in viability during the stationary phase. There were no detectable levels of H2O2 in cultures without agitation. H2O2-producing lactobacilli inhibited growth of S. aureus in a plaque assay and in mixed cultures, depending on the initial inoculum of the pathogen.</description><identifier>ISSN: 0928-8244</identifier><identifier>EISSN: 1574-695X</identifier><identifier>EISSN: 2049-632X</identifier><identifier>DOI: 10.1111/j.1574-695X.1999.tb01227.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Cell culture ; Hydrogen peroxide ; Inoculum ; Lactobacilli ; Lactobacillus paracasei ; Metabolites ; Plaque assay ; Staphylococcus aureus ; Stationary phase ; Strains (organisms) ; Vagina ; Vaginal lactobacilli ; Viability</subject><ispartof>FEMS immunology and medical microbiology, 1999-02, Vol.23 (2), p.87-92</ispartof><rights>1999 Federation of European Microbiological Societies. 1999</rights><rights>1999 Federation of European Microbiological Societies.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-695X.1999.tb01227.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-695X.1999.tb01227.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Ocaña, Virginia S.</creatorcontrib><creatorcontrib>Pesce de Ruiz Holgado, Aída A.</creatorcontrib><creatorcontrib>Nader-Macías, María Elena</creatorcontrib><title>Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina</title><title>FEMS immunology and medical microbiology</title><description>Abstract
H2O2 production by certain Lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. This paper describes the kinetics of H2O2 production by two different strains of Lactobacillus paracasei subsp. paracasei under different culture conditions and the effect of this metabolite on the growth of Staphylococcus aureus. L. paracasei F2 produced 2.72 mmol l−1 H2O2 while L. paracasei F28 produced 1.84 mmol l−1, both in agitated cultures. Although L. paracasei F2 produced a higher H2O2 concentration than L. paracasei F28, H2O2 production per number of live bacterial cells was 10-fold higher for F28. The latter also showed a faster decrease in viability during the stationary phase. There were no detectable levels of H2O2 in cultures without agitation. H2O2-producing lactobacilli inhibited growth of S. aureus in a plaque assay and in mixed cultures, depending on the initial inoculum of the pathogen.</description><subject>Cell culture</subject><subject>Hydrogen peroxide</subject><subject>Inoculum</subject><subject>Lactobacilli</subject><subject>Lactobacillus paracasei</subject><subject>Metabolites</subject><subject>Plaque assay</subject><subject>Staphylococcus aureus</subject><subject>Stationary phase</subject><subject>Strains (organisms)</subject><subject>Vagina</subject><subject>Vaginal lactobacilli</subject><subject>Viability</subject><issn>0928-8244</issn><issn>1574-695X</issn><issn>2049-632X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1999</creationdate><recordtype>article</recordtype><recordid>eNp1kEFv2zAMhYWhBZa1-w_CdrZHybYsH3YYgiUNkKGHtkBvAiVLtQLH8ix7Te774XOQYuih5YUA33sk-BHyhUHK5vq2S1lR5omoiseUVVWVjhoY52V6-EAW_6ULsoCKy0TyPP9IPsW4A4C8AliQv-shPI8N9V3jtR996Ghw9G7Evjm2wQRjpkhxGuzc9JHe8Fue9EOoJ-O7J7pFMwaNxrftrPc4oMFoPY2Tjn36auBjaHG0NXVD2NOxsbSZ9tjRP_jkO7wmlw7baD-_9CvysPp5v7xJtrfrzfLHNgmMyzLJEXWZ5dIVAAa0rVnhMgFCS8ZNDtqYvBSFFmXtEFxhnZActGPGSaaxttkV-XreO3_we7JxVLswDd18UvEs4yCElGJ2fT-7nn1rj6of_B6Ho2KgTsjVTp24qhNXdUKuXpCrg1ptfslyzhfnfJj6d9LJG-nsH66Bi0Y</recordid><startdate>19990201</startdate><enddate>19990201</enddate><creator>Ocaña, Virginia S.</creator><creator>Pesce de Ruiz Holgado, Aída A.</creator><creator>Nader-Macías, María Elena</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope></search><sort><creationdate>19990201</creationdate><title>Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina</title><author>Ocaña, Virginia S. ; Pesce de Ruiz Holgado, Aída A. ; Nader-Macías, María Elena</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o1287-4aab7348f500c0bed15f3606b812c40bcc4765b67dfa0f5ef6820bf1cf81bade3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1999</creationdate><topic>Cell culture</topic><topic>Hydrogen peroxide</topic><topic>Inoculum</topic><topic>Lactobacilli</topic><topic>Lactobacillus paracasei</topic><topic>Metabolites</topic><topic>Plaque assay</topic><topic>Staphylococcus aureus</topic><topic>Stationary phase</topic><topic>Strains (organisms)</topic><topic>Vagina</topic><topic>Vaginal lactobacilli</topic><topic>Viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ocaña, Virginia S.</creatorcontrib><creatorcontrib>Pesce de Ruiz Holgado, Aída A.</creatorcontrib><creatorcontrib>Nader-Macías, María Elena</creatorcontrib><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>FEMS immunology and medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ocaña, Virginia S.</au><au>Pesce de Ruiz Holgado, Aída A.</au><au>Nader-Macías, María Elena</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina</atitle><jtitle>FEMS immunology and medical microbiology</jtitle><date>1999-02-01</date><risdate>1999</risdate><volume>23</volume><issue>2</issue><spage>87</spage><epage>92</epage><pages>87-92</pages><issn>0928-8244</issn><eissn>1574-695X</eissn><eissn>2049-632X</eissn><abstract>Abstract
H2O2 production by certain Lactobacillus strains is one of the mechanisms that helps to regulate the vaginal ecosystem. This paper describes the kinetics of H2O2 production by two different strains of Lactobacillus paracasei subsp. paracasei under different culture conditions and the effect of this metabolite on the growth of Staphylococcus aureus. L. paracasei F2 produced 2.72 mmol l−1 H2O2 while L. paracasei F28 produced 1.84 mmol l−1, both in agitated cultures. Although L. paracasei F2 produced a higher H2O2 concentration than L. paracasei F28, H2O2 production per number of live bacterial cells was 10-fold higher for F28. The latter also showed a faster decrease in viability during the stationary phase. There were no detectable levels of H2O2 in cultures without agitation. H2O2-producing lactobacilli inhibited growth of S. aureus in a plaque assay and in mixed cultures, depending on the initial inoculum of the pathogen.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-695X.1999.tb01227.x</doi><tpages>6</tpages></addata></record> |
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| ispartof | FEMS immunology and medical microbiology, 1999-02, Vol.23 (2), p.87-92 |
| issn | 0928-8244 1574-695X 2049-632X |
| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Cell culture Hydrogen peroxide Inoculum Lactobacilli Lactobacillus paracasei Metabolites Plaque assay Staphylococcus aureus Stationary phase Strains (organisms) Vagina Vaginal lactobacilli Viability |
| title | Growth inhibition of Staphylococcus aureus by H2O2-producing Lactobacillus paracasei subsp. paracasei isolated from the human vagina |
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