Survival and conjugation of Bacillus thuringiensis in a soil microcosm
Abstract The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology ecology 2000-03, Vol.31 (3), p.255-259 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 259 |
|---|---|
| container_issue | 3 |
| container_start_page | 255 |
| container_title | FEMS microbiology ecology |
| container_volume | 31 |
| creator | Vilas-Bôas, Laurival A. Vilas-Bôas, Gislayne F.L.T. Saridakis, Halha O. Lemos, Manoel Victor F. Lereclu, Didier Arantes, Olivia M.N. |
| description | Abstract
The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment. |
| doi_str_mv | 10.1111/j.1574-6941.2000.tb00691.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2331811031</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6941.2000.tb00691.x</oup_id><sourcerecordid>2331811031</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3795-4b8d78ba440a435a77b541e08e0ea4f5dbf1f4825e71a96e100bb2e0a92813dc3</originalsourceid><addsrcrecordid>eNqVkD1PwzAQhi0EEqXwHyyYE-5iOx8MSFC1gFTEAMyWkzjFURqXOCntvydRKiYYuOWGez9ODyGXCD72c136KCLuhQlHPwAAv00BwgT93RGZ_JyOyQQwjL2QJ-EpOXOuBEDBOEzI4rVrtmarKqrqnGa2LruVao2tqS3ovcpMVXWOth9dY-qV0bUzjpqaKuqsqejaZI3NrFufk5NCVU5fHPaUvC_mb7NHb_ny8DS7W3oZixLh8TTOozhVnIPiTKgoSgVHDbEGrXgh8rTAgseB0BGqJNQIkKaBBpUEMbI8Y1NyNeZuGvvZadfK0nZN3VfKgDGMEYFhr7oZVf13zjW6kJvGrFWzlwhy4CZLOcCRAxw5cJMHbnLXm29H85ep9P4fTrmYPwdC9AFiDLDd5g-791vxN2ZMhM8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2331811031</pqid></control><display><type>article</type><title>Survival and conjugation of Bacillus thuringiensis in a soil microcosm</title><source>Access via Oxford University Press (Open Access Collection)</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Access via Wiley Online Library</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><creator>Vilas-Bôas, Laurival A. ; Vilas-Bôas, Gislayne F.L.T. ; Saridakis, Halha O. ; Lemos, Manoel Victor F. ; Lereclu, Didier ; Arantes, Olivia M.N.</creator><creatorcontrib>Vilas-Bôas, Laurival A. ; Vilas-Bôas, Gislayne F.L.T. ; Saridakis, Halha O. ; Lemos, Manoel Victor F. ; Lereclu, Didier ; Arantes, Olivia M.N.</creatorcontrib><description>Abstract
The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment.</description><identifier>ISSN: 0168-6496</identifier><identifier>EISSN: 1574-6941</identifier><identifier>DOI: 10.1111/j.1574-6941.2000.tb00691.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacillus thuringiensis ; Clay soils ; Conjugation ; Contamination ; Cry1Ac gene ; Cry1Ac toxin ; Ecology ; Environmental impact ; Erythromycin ; Gene transfer ; Microbiology ; Monoculture ; Pesticides ; Plasmid ; Soil contamination ; Soil investigations ; Soil microcosm ; Soil pollution ; Spores ; Sporulation mutant ; Survival</subject><ispartof>FEMS microbiology ecology, 2000-03, Vol.31 (3), p.255-259</ispartof><rights>2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. 2000</rights><rights>2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3795-4b8d78ba440a435a77b541e08e0ea4f5dbf1f4825e71a96e100bb2e0a92813dc3</citedby><cites>FETCH-LOGICAL-c3795-4b8d78ba440a435a77b541e08e0ea4f5dbf1f4825e71a96e100bb2e0a92813dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6941.2000.tb00691.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6941.2000.tb00691.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27933,27934,45583,45584</link.rule.ids></links><search><creatorcontrib>Vilas-Bôas, Laurival A.</creatorcontrib><creatorcontrib>Vilas-Bôas, Gislayne F.L.T.</creatorcontrib><creatorcontrib>Saridakis, Halha O.</creatorcontrib><creatorcontrib>Lemos, Manoel Victor F.</creatorcontrib><creatorcontrib>Lereclu, Didier</creatorcontrib><creatorcontrib>Arantes, Olivia M.N.</creatorcontrib><title>Survival and conjugation of Bacillus thuringiensis in a soil microcosm</title><title>FEMS microbiology ecology</title><description>Abstract
The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment.</description><subject>Bacillus thuringiensis</subject><subject>Clay soils</subject><subject>Conjugation</subject><subject>Contamination</subject><subject>Cry1Ac gene</subject><subject>Cry1Ac toxin</subject><subject>Ecology</subject><subject>Environmental impact</subject><subject>Erythromycin</subject><subject>Gene transfer</subject><subject>Microbiology</subject><subject>Monoculture</subject><subject>Pesticides</subject><subject>Plasmid</subject><subject>Soil contamination</subject><subject>Soil investigations</subject><subject>Soil microcosm</subject><subject>Soil pollution</subject><subject>Spores</subject><subject>Sporulation mutant</subject><subject>Survival</subject><issn>0168-6496</issn><issn>1574-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqVkD1PwzAQhi0EEqXwHyyYE-5iOx8MSFC1gFTEAMyWkzjFURqXOCntvydRKiYYuOWGez9ODyGXCD72c136KCLuhQlHPwAAv00BwgT93RGZ_JyOyQQwjL2QJ-EpOXOuBEDBOEzI4rVrtmarKqrqnGa2LruVao2tqS3ovcpMVXWOth9dY-qV0bUzjpqaKuqsqejaZI3NrFufk5NCVU5fHPaUvC_mb7NHb_ny8DS7W3oZixLh8TTOozhVnIPiTKgoSgVHDbEGrXgh8rTAgseB0BGqJNQIkKaBBpUEMbI8Y1NyNeZuGvvZadfK0nZN3VfKgDGMEYFhr7oZVf13zjW6kJvGrFWzlwhy4CZLOcCRAxw5cJMHbnLXm29H85ep9P4fTrmYPwdC9AFiDLDd5g-791vxN2ZMhM8</recordid><startdate>20000301</startdate><enddate>20000301</enddate><creator>Vilas-Bôas, Laurival A.</creator><creator>Vilas-Bôas, Gislayne F.L.T.</creator><creator>Saridakis, Halha O.</creator><creator>Lemos, Manoel Victor F.</creator><creator>Lereclu, Didier</creator><creator>Arantes, Olivia M.N.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope></search><sort><creationdate>20000301</creationdate><title>Survival and conjugation of Bacillus thuringiensis in a soil microcosm</title><author>Vilas-Bôas, Laurival A. ; Vilas-Bôas, Gislayne F.L.T. ; Saridakis, Halha O. ; Lemos, Manoel Victor F. ; Lereclu, Didier ; Arantes, Olivia M.N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3795-4b8d78ba440a435a77b541e08e0ea4f5dbf1f4825e71a96e100bb2e0a92813dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Bacillus thuringiensis</topic><topic>Clay soils</topic><topic>Conjugation</topic><topic>Contamination</topic><topic>Cry1Ac gene</topic><topic>Cry1Ac toxin</topic><topic>Ecology</topic><topic>Environmental impact</topic><topic>Erythromycin</topic><topic>Gene transfer</topic><topic>Microbiology</topic><topic>Monoculture</topic><topic>Pesticides</topic><topic>Plasmid</topic><topic>Soil contamination</topic><topic>Soil investigations</topic><topic>Soil microcosm</topic><topic>Soil pollution</topic><topic>Spores</topic><topic>Sporulation mutant</topic><topic>Survival</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilas-Bôas, Laurival A.</creatorcontrib><creatorcontrib>Vilas-Bôas, Gislayne F.L.T.</creatorcontrib><creatorcontrib>Saridakis, Halha O.</creatorcontrib><creatorcontrib>Lemos, Manoel Victor F.</creatorcontrib><creatorcontrib>Lereclu, Didier</creatorcontrib><creatorcontrib>Arantes, Olivia M.N.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology ecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilas-Bôas, Laurival A.</au><au>Vilas-Bôas, Gislayne F.L.T.</au><au>Saridakis, Halha O.</au><au>Lemos, Manoel Victor F.</au><au>Lereclu, Didier</au><au>Arantes, Olivia M.N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Survival and conjugation of Bacillus thuringiensis in a soil microcosm</atitle><jtitle>FEMS microbiology ecology</jtitle><date>2000-03-01</date><risdate>2000</risdate><volume>31</volume><issue>3</issue><spage>255</spage><epage>259</epage><pages>255-259</pages><issn>0168-6496</issn><eissn>1574-6941</eissn><abstract>Abstract
The survival and conjugation ability of sporogenic and asporogenic Bacillus thuringiensis strains were investigated in broth, in non-amended sterile clay soil monoculture and in mixed soil culture. The 75 kb pHT73 plasmid carrying an erythromycin resistance determinant and a cry1Ac gene was transferred in mating broth and soil microcosm. Survival of strains was assessed in soil monoculture and in mixed soil culture for up to 20 days. Sporogenic strains rapidly formed viable spores which were maintained until the end of the experiment. The asporogenic strains were no longer recovered after 8 days of incubation. This study shows that the environmental impact of asporogenic B. thuringiensis strains is lower than that of sporogenic B. thuringiensis strains. Thus, the use of asporogenic strains may significantly reduce any potential risk (gene transfer, soil and plant contamination) due to the dissemination of B. thuringiensis-based biopesticides in the environment.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6941.2000.tb00691.x</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0168-6496 |
| ispartof | FEMS microbiology ecology, 2000-03, Vol.31 (3), p.255-259 |
| issn | 0168-6496 1574-6941 |
| language | eng |
| recordid | cdi_proquest_journals_2331811031 |
| source | Access via Oxford University Press (Open Access Collection); Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Bacillus thuringiensis Clay soils Conjugation Contamination Cry1Ac gene Cry1Ac toxin Ecology Environmental impact Erythromycin Gene transfer Microbiology Monoculture Pesticides Plasmid Soil contamination Soil investigations Soil microcosm Soil pollution Spores Sporulation mutant Survival |
| title | Survival and conjugation of Bacillus thuringiensis in a soil microcosm |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-02T21%3A58%3A20IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Survival%20and%20conjugation%20of%20Bacillus%20thuringiensis%20in%20a%20soil%20microcosm&rft.jtitle=FEMS%20microbiology%20ecology&rft.au=Vilas-B%C3%B4as,%20Laurival%20A.&rft.date=2000-03-01&rft.volume=31&rft.issue=3&rft.spage=255&rft.epage=259&rft.pages=255-259&rft.issn=0168-6496&rft.eissn=1574-6941&rft_id=info:doi/10.1111/j.1574-6941.2000.tb00691.x&rft_dat=%3Cproquest_cross%3E2331811031%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2331811031&rft_id=info:pmid/&rft_oup_id=10.1111/j.1574-6941.2000.tb00691.x&rfr_iscdi=true |