RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas
Abstract The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxy...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 1997-11, Vol.156 (2), p.199-204 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 204 |
|---|---|
| container_issue | 2 |
| container_start_page | 199 |
| container_title | FEMS microbiology letters |
| container_volume | 156 |
| creator | Cascón Soriano, Alberto Anguita Castillo, Juan Hernanz Moral, Carmen Sánchez Salazar, Marıća Yugueros Marcos, Javier Naharro Carrasco, Germán |
| description | Abstract
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. A 1236-bp DNA fragment, representing most of the aroA gene, according to the nucleotide sequence of A. salmonicida, was amplified from all Aeromonas species tested, which represented most of the 14 hybridization groups. HaeII digestion of the 1236-bp fragment generated a restriction fragment length polymorphisms which could be used as a powerful tool for identification of aeromonads to the genus level. |
| doi_str_mv | 10.1111/j.1574-6968.1997.tb12727.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2331801082</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.1997.tb12727.x</oup_id><sourcerecordid>2331801082</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2899-f9a9d59c88307fd84922e0747826366aea981b7c4228d1d1695205f18c49498b3</originalsourceid><addsrcrecordid>eNqVkF9LwzAUxYMoOKffIehza5Km-eODMIZToeoQfQ5Zm2pL18ykxe3bm9rhkz54X-4l95xzyQ-Ac4xiHOqyjnHKacQkEzGWksfdChNOeLw9AJOf1SGYoISLCCPJj8GJ9zVCiBLEJuDxeZEto-X8GepWNztfeWhL2L0bqJ2dwTfThslDDTu9ta1dVznsrG1gad23Kgh6D2fG2bVttT8FR6VuvDnb9yl4Xdy8zO-i7On2fj7LopwIKaNSalmkMhciQbwsBJWEGMQpF4QljGmjpcArnlNCRIELzGRKUFpikVNJpVglU3Ax5m6c_eiN71Rtexd-4BVJEiwQRoIE1dWoyp313plSbVy11m6nMFIDP1WrAZIaIKmBn9rzU9tgvh7Nn1Vjdv9wqsVDFl5CQDoG2H7zhz367fAXzxuFPw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2331801082</pqid></control><display><type>article</type><title>RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas</title><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><creator>Cascón Soriano, Alberto ; Anguita Castillo, Juan ; Hernanz Moral, Carmen ; Sánchez Salazar, Marıća ; Yugueros Marcos, Javier ; Naharro Carrasco, Germán</creator><creatorcontrib>Cascón Soriano, Alberto ; Anguita Castillo, Juan ; Hernanz Moral, Carmen ; Sánchez Salazar, Marıća ; Yugueros Marcos, Javier ; Naharro Carrasco, Germán</creatorcontrib><description>Abstract
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. A 1236-bp DNA fragment, representing most of the aroA gene, according to the nucleotide sequence of A. salmonicida, was amplified from all Aeromonas species tested, which represented most of the 14 hybridization groups. HaeII digestion of the 1236-bp fragment generated a restriction fragment length polymorphisms which could be used as a powerful tool for identification of aeromonads to the genus level.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1997.tb12727.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Aeromonads ; Aeromonas ; Amino acids ; Amplification ; Deoxyribonucleic acid ; DNA ; Folic acid ; Hybridization ; Identification ; Microbiology ; Nucleotide sequence ; Nucleotides ; Oligonucleotides ; PCR‐RFLP ; Polymerase chain reaction ; Target recognition</subject><ispartof>FEMS microbiology letters, 1997-11, Vol.156 (2), p.199-204</ispartof><rights>1997 Federation of European Microbiological Societies. Published by Elsevier Science B.V. 1997</rights><rights>1997 Federation of European Microbiological Societies. Published by Elsevier Science B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2899-f9a9d59c88307fd84922e0747826366aea981b7c4228d1d1695205f18c49498b3</citedby><cites>FETCH-LOGICAL-c2899-f9a9d59c88307fd84922e0747826366aea981b7c4228d1d1695205f18c49498b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1997.tb12727.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1997.tb12727.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids></links><search><creatorcontrib>Cascón Soriano, Alberto</creatorcontrib><creatorcontrib>Anguita Castillo, Juan</creatorcontrib><creatorcontrib>Hernanz Moral, Carmen</creatorcontrib><creatorcontrib>Sánchez Salazar, Marıća</creatorcontrib><creatorcontrib>Yugueros Marcos, Javier</creatorcontrib><creatorcontrib>Naharro Carrasco, Germán</creatorcontrib><title>RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas</title><title>FEMS microbiology letters</title><description>Abstract
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. A 1236-bp DNA fragment, representing most of the aroA gene, according to the nucleotide sequence of A. salmonicida, was amplified from all Aeromonas species tested, which represented most of the 14 hybridization groups. HaeII digestion of the 1236-bp fragment generated a restriction fragment length polymorphisms which could be used as a powerful tool for identification of aeromonads to the genus level.</description><subject>Aeromonads</subject><subject>Aeromonas</subject><subject>Amino acids</subject><subject>Amplification</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Folic acid</subject><subject>Hybridization</subject><subject>Identification</subject><subject>Microbiology</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>Oligonucleotides</subject><subject>PCR‐RFLP</subject><subject>Polymerase chain reaction</subject><subject>Target recognition</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNqVkF9LwzAUxYMoOKffIehza5Km-eODMIZToeoQfQ5Zm2pL18ykxe3bm9rhkz54X-4l95xzyQ-Ac4xiHOqyjnHKacQkEzGWksfdChNOeLw9AJOf1SGYoISLCCPJj8GJ9zVCiBLEJuDxeZEto-X8GepWNztfeWhL2L0bqJ2dwTfThslDDTu9ta1dVznsrG1gad23Kgh6D2fG2bVttT8FR6VuvDnb9yl4Xdy8zO-i7On2fj7LopwIKaNSalmkMhciQbwsBJWEGMQpF4QljGmjpcArnlNCRIELzGRKUFpikVNJpVglU3Ax5m6c_eiN71Rtexd-4BVJEiwQRoIE1dWoyp313plSbVy11m6nMFIDP1WrAZIaIKmBn9rzU9tgvh7Nn1Vjdv9wqsVDFl5CQDoG2H7zhz367fAXzxuFPw</recordid><startdate>19971101</startdate><enddate>19971101</enddate><creator>Cascón Soriano, Alberto</creator><creator>Anguita Castillo, Juan</creator><creator>Hernanz Moral, Carmen</creator><creator>Sánchez Salazar, Marıća</creator><creator>Yugueros Marcos, Javier</creator><creator>Naharro Carrasco, Germán</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>19971101</creationdate><title>RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas</title><author>Cascón Soriano, Alberto ; Anguita Castillo, Juan ; Hernanz Moral, Carmen ; Sánchez Salazar, Marıća ; Yugueros Marcos, Javier ; Naharro Carrasco, Germán</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2899-f9a9d59c88307fd84922e0747826366aea981b7c4228d1d1695205f18c49498b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Aeromonads</topic><topic>Aeromonas</topic><topic>Amino acids</topic><topic>Amplification</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Folic acid</topic><topic>Hybridization</topic><topic>Identification</topic><topic>Microbiology</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>Oligonucleotides</topic><topic>PCR‐RFLP</topic><topic>Polymerase chain reaction</topic><topic>Target recognition</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cascón Soriano, Alberto</creatorcontrib><creatorcontrib>Anguita Castillo, Juan</creatorcontrib><creatorcontrib>Hernanz Moral, Carmen</creatorcontrib><creatorcontrib>Sánchez Salazar, Marıća</creatorcontrib><creatorcontrib>Yugueros Marcos, Javier</creatorcontrib><creatorcontrib>Naharro Carrasco, Germán</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cascón Soriano, Alberto</au><au>Anguita Castillo, Juan</au><au>Hernanz Moral, Carmen</au><au>Sánchez Salazar, Marıća</au><au>Yugueros Marcos, Javier</au><au>Naharro Carrasco, Germán</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas</atitle><jtitle>FEMS microbiology letters</jtitle><date>1997-11-01</date><risdate>1997</risdate><volume>156</volume><issue>2</issue><spage>199</spage><epage>204</epage><pages>199-204</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The aroA gene has been identified as a target in screening for the presence of most Aeromonas species so far described by PCR. Synthetic oligonucleotide primers of 24 and 25 nucleotides were used by PCR assay to amplify a sequence of the aroA gene, which encodes 3-phosphoshikimate-1-carboxyvinyltransferase, a key enzyme of aromatic amino acids and folate biosynthetic pathway. A 1236-bp DNA fragment, representing most of the aroA gene, according to the nucleotide sequence of A. salmonicida, was amplified from all Aeromonas species tested, which represented most of the 14 hybridization groups. HaeII digestion of the 1236-bp fragment generated a restriction fragment length polymorphisms which could be used as a powerful tool for identification of aeromonads to the genus level.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1997.tb12727.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1997-11, Vol.156 (2), p.199-204 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_journals_2331801082 |
| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
| subjects | Aeromonads Aeromonas Amino acids Amplification Deoxyribonucleic acid DNA Folic acid Hybridization Identification Microbiology Nucleotide sequence Nucleotides Oligonucleotides PCR‐RFLP Polymerase chain reaction Target recognition |
| title | RFLP-PCR analysis of the aroA gene as a taxonomic tool for the genus Aeromonas |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-31T22%3A26%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=RFLP-PCR%20analysis%20of%20the%20aroA%20gene%20as%20a%20taxonomic%20tool%20for%20the%20genus%20Aeromonas&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Casc%C3%B3n%20Soriano,%20Alberto&rft.date=1997-11-01&rft.volume=156&rft.issue=2&rft.spage=199&rft.epage=204&rft.pages=199-204&rft.issn=0378-1097&rft.eissn=1574-6968&rft_id=info:doi/10.1111/j.1574-6968.1997.tb12727.x&rft_dat=%3Cproquest_cross%3E2331801082%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2331801082&rft_id=info:pmid/&rft_oup_id=10.1111/j.1574-6968.1997.tb12727.x&rfr_iscdi=true |