Antibacterial activity of biogenic silver nanoparticles synthesized with gum ghatti and gum olibanum: a comparative study
Presently, silver nanoparticles produced by biological methods have received considerable significance owing to the natural abundance of renewable, cost-effective and biodegradable materials, thus implementing the green chemistry principles. Compared with the nanoparticles synthesized using chemical...
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| Veröffentlicht in: | Journal of antibiotics 2015-02, Vol.68 (2), p.88-97 |
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| creator | Kora, Aruna Jyothi Sashidhar, Rao Beedu |
| description | Presently, silver nanoparticles produced by biological methods have received considerable significance owing to the natural abundance of renewable, cost-effective and biodegradable materials, thus implementing the green chemistry principles. Compared with the nanoparticles synthesized using chemical methods, most biogenic silver nanoparticles are protein capped, which imparts stability and biocompatibility, and enhanced antibacterial activity. In this study, we compared the antibacterial effect of two biogenic silver nanoparticles produced with natural plant gums: gum ghatti and gum olibanum against Gram-negative and Gram-positive bacteria. Bacterial interaction with nanoparticles was probed both in planktonic and biofilm modes of growth; employing solid agar and liquid broth assays for inhibition zone, antibiofilm activity, inhibition of growth kinetics, leakage of intracellular contents, membrane permeabilization and reactive oxygen species production. In addition, cytotoxicity of the biogenic nanoparticles was evaluated in HeLa cells, a human carcinoma cell line. Antibacterial activity and cytotoxicity of the silver nanoparticles synthesized with gum ghatti (Ag NP-GT) was greater than that produced with gum olibanum (Ag NP-OB). This could be attributed to the smaller size (5.7 nm), monodispersity and zeta potential of the Ag NP-GT. The study suggests that Ag NP-GT can be employed as a cytotoxic bactericidal agent, whereas Ag NP-OB (7.5 nm) as a biocompatible bactericidal agent. |
| doi_str_mv | 10.1038/ja.2014.114 |
| format | Article |
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Compared with the nanoparticles synthesized using chemical methods, most biogenic silver nanoparticles are protein capped, which imparts stability and biocompatibility, and enhanced antibacterial activity. In this study, we compared the antibacterial effect of two biogenic silver nanoparticles produced with natural plant gums: gum ghatti and gum olibanum against Gram-negative and Gram-positive bacteria. Bacterial interaction with nanoparticles was probed both in planktonic and biofilm modes of growth; employing solid agar and liquid broth assays for inhibition zone, antibiofilm activity, inhibition of growth kinetics, leakage of intracellular contents, membrane permeabilization and reactive oxygen species production. In addition, cytotoxicity of the biogenic nanoparticles was evaluated in HeLa cells, a human carcinoma cell line. Antibacterial activity and cytotoxicity of the silver nanoparticles synthesized with gum ghatti (Ag NP-GT) was greater than that produced with gum olibanum (Ag NP-OB). This could be attributed to the smaller size (5.7 nm), monodispersity and zeta potential of the Ag NP-GT. The study suggests that Ag NP-GT can be employed as a cytotoxic bactericidal agent, whereas Ag NP-OB (7.5 nm) as a biocompatible bactericidal agent.</description><identifier>ISSN: 0021-8820</identifier><identifier>EISSN: 1881-1469</identifier><identifier>DOI: 10.1038/ja.2014.114</identifier><identifier>PMID: 25138141</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/154 ; 631/326/22/1290 ; 631/61/350/354 ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Anti-Bacterial Agents - toxicity ; Bacteria ; Bacteriology ; Biodegradable materials ; Biodegradation ; Biofilms ; Biofilms - drug effects ; Biomedical and Life Sciences ; Bioorganic Chemistry ; Comparative studies ; Cytotoxicity ; Frankincense - chemistry ; Gram-Negative Bacteria - drug effects ; Gram-positive bacteria ; Gram-Positive Bacteria - drug effects ; Green chemistry ; Growth kinetics ; HeLa Cells ; Humans ; Life Sciences ; Medicinal Chemistry ; Metal Nanoparticles ; Microbiology ; Nanoparticles ; Organic Chemistry ; original-article ; Plankton - microbiology ; Plant Gums - chemistry ; Reactive oxygen species ; Silver ; Silver - chemistry ; Zeta potential</subject><ispartof>Journal of antibiotics, 2015-02, Vol.68 (2), p.88-97</ispartof><rights>Japan Antibiotics Research Association 2015</rights><rights>Copyright Nature Publishing Group Feb 2015</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-bce8f0b52104ef83011cb3f116daedaa6f612f6d596bcba7e0e51696a69c680d3</citedby><cites>FETCH-LOGICAL-c443t-bce8f0b52104ef83011cb3f116daedaa6f612f6d596bcba7e0e51696a69c680d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25138141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kora, Aruna Jyothi</creatorcontrib><creatorcontrib>Sashidhar, Rao Beedu</creatorcontrib><title>Antibacterial activity of biogenic silver nanoparticles synthesized with gum ghatti and gum olibanum: a comparative study</title><title>Journal of antibiotics</title><addtitle>J Antibiot</addtitle><addtitle>J Antibiot (Tokyo)</addtitle><description>Presently, silver nanoparticles produced by biological methods have received considerable significance owing to the natural abundance of renewable, cost-effective and biodegradable materials, thus implementing the green chemistry principles. Compared with the nanoparticles synthesized using chemical methods, most biogenic silver nanoparticles are protein capped, which imparts stability and biocompatibility, and enhanced antibacterial activity. In this study, we compared the antibacterial effect of two biogenic silver nanoparticles produced with natural plant gums: gum ghatti and gum olibanum against Gram-negative and Gram-positive bacteria. Bacterial interaction with nanoparticles was probed both in planktonic and biofilm modes of growth; employing solid agar and liquid broth assays for inhibition zone, antibiofilm activity, inhibition of growth kinetics, leakage of intracellular contents, membrane permeabilization and reactive oxygen species production. In addition, cytotoxicity of the biogenic nanoparticles was evaluated in HeLa cells, a human carcinoma cell line. Antibacterial activity and cytotoxicity of the silver nanoparticles synthesized with gum ghatti (Ag NP-GT) was greater than that produced with gum olibanum (Ag NP-OB). This could be attributed to the smaller size (5.7 nm), monodispersity and zeta potential of the Ag NP-GT. 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Compared with the nanoparticles synthesized using chemical methods, most biogenic silver nanoparticles are protein capped, which imparts stability and biocompatibility, and enhanced antibacterial activity. In this study, we compared the antibacterial effect of two biogenic silver nanoparticles produced with natural plant gums: gum ghatti and gum olibanum against Gram-negative and Gram-positive bacteria. Bacterial interaction with nanoparticles was probed both in planktonic and biofilm modes of growth; employing solid agar and liquid broth assays for inhibition zone, antibiofilm activity, inhibition of growth kinetics, leakage of intracellular contents, membrane permeabilization and reactive oxygen species production. In addition, cytotoxicity of the biogenic nanoparticles was evaluated in HeLa cells, a human carcinoma cell line. Antibacterial activity and cytotoxicity of the silver nanoparticles synthesized with gum ghatti (Ag NP-GT) was greater than that produced with gum olibanum (Ag NP-OB). This could be attributed to the smaller size (5.7 nm), monodispersity and zeta potential of the Ag NP-GT. The study suggests that Ag NP-GT can be employed as a cytotoxic bactericidal agent, whereas Ag NP-OB (7.5 nm) as a biocompatible bactericidal agent.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>25138141</pmid><doi>10.1038/ja.2014.114</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 631/154 631/326/22/1290 631/61/350/354 Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Anti-Bacterial Agents - toxicity Bacteria Bacteriology Biodegradable materials Biodegradation Biofilms Biofilms - drug effects Biomedical and Life Sciences Bioorganic Chemistry Comparative studies Cytotoxicity Frankincense - chemistry Gram-Negative Bacteria - drug effects Gram-positive bacteria Gram-Positive Bacteria - drug effects Green chemistry Growth kinetics HeLa Cells Humans Life Sciences Medicinal Chemistry Metal Nanoparticles Microbiology Nanoparticles Organic Chemistry original-article Plankton - microbiology Plant Gums - chemistry Reactive oxygen species Silver Silver - chemistry Zeta potential |
| title | Antibacterial activity of biogenic silver nanoparticles synthesized with gum ghatti and gum olibanum: a comparative study |
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