Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp
Abstract The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp....
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 1998-08, Vol.165 (2), p.357-362 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 362 |
|---|---|
| container_issue | 2 |
| container_start_page | 357 |
| container_title | FEMS microbiology letters |
| container_volume | 165 |
| creator | Jawad, A. Snelling, A.M. Heritage, J. Hawkey, P.M. |
| description | Abstract
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined. |
| doi_str_mv | 10.1111/j.1574-6968.1998.tb13170.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_wiley</sourceid><recordid>TN_cdi_proquest_journals_2331464258</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1111/j.1574-6968.1998.tb13170.x</oup_id><sourcerecordid>2331464258</sourcerecordid><originalsourceid>FETCH-LOGICAL-o1297-b8bc5682f2b773a052198ab6461b521b0953ae53e86971cb00296e72978cbedf3</originalsourceid><addsrcrecordid>eNp1kF1LwzAUhoMoOKf_oeh1az7afNwIZToVJsrQ65BkqaRsTU063P69KRte6bk553De9-XwAHCNYIFS3bYFqliZU0F5gYTgxaARQQwWuxMw-T2dggkkjOcICnYOLmJsIYQlhnQC9MxvehVc9F3mm6xe3i_rTHWrLFhT58v54i1tar2PLmaND9mn7fzGmSz21jgbM7ey3eAaZ9TgjhHGdXbwWpnBhqTrL8FZo9bRXh37FHzMH95nT_ni9fF5Vi9yj7BguebaVJTjBmvGiIIVRoIrTUuKdJo1FBVRtiKWU8GQ0RBiQS1LVm60XTVkCm4OuX3wX1sbB9n6bUjPR4kJQSUtccWT6u6g-nZru5d9cBsV9hJBOQKVrRypyZGaHIHKI1C5k_OXBalYCqgOAX7b_2PP_7CTHy0We54</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2331464258</pqid></control><display><type>article</type><title>Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp</title><source>Wiley Online Library Journals Frontfile Complete</source><source>Alma/SFX Local Collection</source><source>Oxford Journals</source><creator>Jawad, A. ; Snelling, A.M. ; Heritage, J. ; Hawkey, P.M.</creator><creatorcontrib>Jawad, A. ; Snelling, A.M. ; Heritage, J. ; Hawkey, P.M.</creatorcontrib><description>Abstract
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1998.tb13170.x</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acinetobacter ; Amplified ribosomal DNA restriction analysis ; Deoxyribonucleic acid ; DNA ; Genospecies ; Heterogeneity ; Microbiology ; Polymerase chain reaction ; Polymorphism ; recA ; RecA protein ; Restriction fragment length polymorphism ; Ribosomal DNA</subject><ispartof>FEMS microbiology letters, 1998-08, Vol.165 (2), p.357-362</ispartof><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved 1998</rights><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1998.tb13170.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1998.tb13170.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids></links><search><creatorcontrib>Jawad, A.</creatorcontrib><creatorcontrib>Snelling, A.M.</creatorcontrib><creatorcontrib>Heritage, J.</creatorcontrib><creatorcontrib>Hawkey, P.M.</creatorcontrib><title>Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp</title><title>FEMS microbiology letters</title><description>Abstract
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined.</description><subject>Acinetobacter</subject><subject>Amplified ribosomal DNA restriction analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Genospecies</subject><subject>Heterogeneity</subject><subject>Microbiology</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>recA</subject><subject>RecA protein</subject><subject>Restriction fragment length polymorphism</subject><subject>Ribosomal DNA</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kF1LwzAUhoMoOKf_oeh1az7afNwIZToVJsrQ65BkqaRsTU063P69KRte6bk553De9-XwAHCNYIFS3bYFqliZU0F5gYTgxaARQQwWuxMw-T2dggkkjOcICnYOLmJsIYQlhnQC9MxvehVc9F3mm6xe3i_rTHWrLFhT58v54i1tar2PLmaND9mn7fzGmSz21jgbM7ey3eAaZ9TgjhHGdXbwWpnBhqTrL8FZo9bRXh37FHzMH95nT_ni9fF5Vi9yj7BguebaVJTjBmvGiIIVRoIrTUuKdJo1FBVRtiKWU8GQ0RBiQS1LVm60XTVkCm4OuX3wX1sbB9n6bUjPR4kJQSUtccWT6u6g-nZru5d9cBsV9hJBOQKVrRypyZGaHIHKI1C5k_OXBalYCqgOAX7b_2PP_7CTHy0We54</recordid><startdate>19980801</startdate><enddate>19980801</enddate><creator>Jawad, A.</creator><creator>Snelling, A.M.</creator><creator>Heritage, J.</creator><creator>Hawkey, P.M.</creator><general>Blackwell Publishing Ltd</general><general>Oxford University Press</general><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>19980801</creationdate><title>Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp</title><author>Jawad, A. ; Snelling, A.M. ; Heritage, J. ; Hawkey, P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-o1297-b8bc5682f2b773a052198ab6461b521b0953ae53e86971cb00296e72978cbedf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Acinetobacter</topic><topic>Amplified ribosomal DNA restriction analysis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Genospecies</topic><topic>Heterogeneity</topic><topic>Microbiology</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>recA</topic><topic>RecA protein</topic><topic>Restriction fragment length polymorphism</topic><topic>Ribosomal DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jawad, A.</creatorcontrib><creatorcontrib>Snelling, A.M.</creatorcontrib><creatorcontrib>Heritage, J.</creatorcontrib><creatorcontrib>Hawkey, P.M.</creatorcontrib><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest - Health & Medical Complete保健、医学与药学数据库</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jawad, A.</au><au>Snelling, A.M.</au><au>Heritage, J.</au><au>Hawkey, P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp</atitle><jtitle>FEMS microbiology letters</jtitle><date>1998-08-01</date><risdate>1998</risdate><volume>165</volume><issue>2</issue><spage>357</spage><epage>362</epage><pages>357-362</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1998.tb13170.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1998-08, Vol.165 (2), p.357-362 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_journals_2331464258 |
| source | Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford Journals |
| subjects | Acinetobacter Amplified ribosomal DNA restriction analysis Deoxyribonucleic acid DNA Genospecies Heterogeneity Microbiology Polymerase chain reaction Polymorphism recA RecA protein Restriction fragment length polymorphism Ribosomal DNA |
| title | Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T15%3A44%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_wiley&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20ARDRA%20and%20recA-RFLP%20analysis%20for%20genomic%20species%20identification%20of%20Acinetobacter%20spp&rft.jtitle=FEMS%20microbiology%20letters&rft.au=Jawad,%20A.&rft.date=1998-08-01&rft.volume=165&rft.issue=2&rft.spage=357&rft.epage=362&rft.pages=357-362&rft.issn=0378-1097&rft.eissn=1574-6968&rft_id=info:doi/10.1111/j.1574-6968.1998.tb13170.x&rft_dat=%3Cproquest_wiley%3E2331464258%3C/proquest_wiley%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2331464258&rft_id=info:pmid/&rft_oup_id=10.1111/j.1574-6968.1998.tb13170.x&rfr_iscdi=true |