Chlorella vulgaris modulates the expression of senescence-associated genes in replicative senescence of human diploid fibroblasts

Human diploid fibroblasts (HDFs) cultured in vitro have limited capacity to proliferate after population doubling is repeated several times, and they enter into a state known as replicative senescence or cellular senescence. This study aimed to investigate the effect of Chlorella vulgaris on the rep...

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Veröffentlicht in:	Molecular biology reports 2020, Vol.47 (1), p.369-379
Hauptverfasser:	Jaafar, Faizul, Durani, Lina Wati, Makpol, Suzana
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Anatomy Animal Biochemistry Antioxidants Antioxidants - metabolism Biomedical and Life Sciences Catalase - genetics Catalase - metabolism CDKN2A gene Cell differentiation Cell Differentiation - drug effects Cell proliferation Cell Proliferation - drug effects Cells, Cultured Cellular Senescence - drug effects Cellular Senescence - genetics Cellular Senescence - physiology Chlorella vulgaris Chlorella vulgaris - metabolism Chlorella vulgaris - pathogenicity Diploidy DNA damage DNA Damage - drug effects Fibroblasts Fibroblasts - metabolism Gene expression Gene Expression - drug effects Genes, p53 - genetics Histology Humans Insulin Insulin-like growth factor I Life Sciences Male Mitogen-Activated Protein Kinase 14 - genetics Molecular Chaperones - genetics Molecular Chaperones - metabolism Morphology Original Article p53 Protein Primary Cell Culture Senescence Signal transduction Signal Transduction - drug effects Superoxide dismutase Superoxide Dismutase - metabolism Superoxide Dismutase-1 - genetics Superoxide Dismutase-1 - metabolism Transcription
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Zusammenfassung:	Human diploid fibroblasts (HDFs) cultured in vitro have limited capacity to proliferate after population doubling is repeated several times, and they enter into a state known as replicative senescence or cellular senescence. This study aimed to investigate the effect of Chlorella vulgaris on the replicative senescence of HDFs by determining the expression of senescence-associated genes. Young and senescent HDFs were divided into untreated control and C. vulgaris -treated groups. A senescence-associated gene transcription analysis was carried out with qRT-PCR. Treatment of young HDFs with C. vulgaris reduced the expression of SOD1 , CAT and CCS (p
ISSN:	0301-4851 1573-4978
DOI:	10.1007/s11033-019-05140-8