Evaluation of rapid methods for the determination of okadaic acid in mussels
L.CROCI, A.STACCHINI, L.COZZI, G.CICCAGLIONI, F.MAZZEI, F.BOTRÈ AND L.TOTI. 2001. Aims: Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the official mouse bioassay in monitoring for okadaic acid (OA) levels in mussels. M...
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| container_title | Journal of applied microbiology |
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| creator | Croci, L. Stacchini, A. Cozzi, L. Ciccaglioni, G. Mazzei, F. Botrè, F. Toti, L. |
| description | L.CROCI, A.STACCHINI, L.COZZI, G.CICCAGLIONI, F.MAZZEI, F.BOTRÈ AND L.TOTI. 2001.
Aims: Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the official mouse bioassay in monitoring for okadaic acid (OA) levels in mussels.
Methods and Results: Diarrhoetic shellfish poison‐contaminated mussels from the Adriatic Sea were assayed in parallel by means of the mouse bioassay and both alternative methods. Both the cytotoxicity test and the biosensor test showed high sensitivity (OA 0·01 mg g–1 hepatopancreas and 0·002 mg g–1 hepatopancreas, respectively) and a high correlation with the mouse bioassay (r=0·932, P |
| doi_str_mv | 10.1046/j.1365-2672.2001.01218.x |
| format | Article |
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Aims: Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the official mouse bioassay in monitoring for okadaic acid (OA) levels in mussels.
Methods and Results: Diarrhoetic shellfish poison‐contaminated mussels from the Adriatic Sea were assayed in parallel by means of the mouse bioassay and both alternative methods. Both the cytotoxicity test and the biosensor test showed high sensitivity (OA 0·01 mg g–1 hepatopancreas and 0·002 mg g–1 hepatopancreas, respectively) and a high correlation with the mouse bioassay (r=0·932, P < 0·001 and r=− 0·850, P < 0·001, respectively).
Conclusions: Both methods are efficacious, quick, inexpensive and provide data on the amount of toxin present in mussels.
Significance and Impact of the Study: Both methods, besides allowing the simultaneous assay of a great number of samples, comply with the ethical need to reduce the use of animals in the laboratory.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1046/j.1365-2672.2001.01218.x</identifier><identifier>PMID: 11155125</identifier><identifier>CODEN: JAMIFK</identifier><language>eng</language><publisher>Oxford UK: Blackwell Science Ltd</publisher><subject>Acid Phosphatase - metabolism ; Animal Testing Alternatives ; Animals ; Biological and medical sciences ; Biological Assay - economics ; Biological Assay - methods ; Biosensing Techniques - economics ; Biosensing Techniques - methods ; Bivalvia - chemistry ; Calibration ; Fish and seafood industries ; Food industries ; Food toxicology ; Fundamental and applied biological sciences. Psychology ; Glucose Oxidase - metabolism ; Haplorhini ; Mice ; Oceans and Seas ; Okadaic Acid - analysis ; Okadaic Acid - toxicity ; Sensitivity and Specificity ; Time Factors ; Toxicity Tests - economics ; Toxicity Tests - methods</subject><ispartof>Journal of applied microbiology, 2001-01, Vol.90 (1), p.73-77</ispartof><rights>2001 INIST-CNRS</rights><rights>Copyright Blackwell Science Ltd. Jan 2001</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4208-73e33719ee3fac2b7ad7eb773b45a9b20907d61dc9b8c6a3b4a6e4f6f1c5890d3</citedby><cites>FETCH-LOGICAL-c4208-73e33719ee3fac2b7ad7eb773b45a9b20907d61dc9b8c6a3b4a6e4f6f1c5890d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1365-2672.2001.01218.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1365-2672.2001.01218.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=868442$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11155125$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Croci, L.</creatorcontrib><creatorcontrib>Stacchini, A.</creatorcontrib><creatorcontrib>Cozzi, L.</creatorcontrib><creatorcontrib>Ciccaglioni, G.</creatorcontrib><creatorcontrib>Mazzei, F.</creatorcontrib><creatorcontrib>Botrè, F.</creatorcontrib><creatorcontrib>Toti, L.</creatorcontrib><title>Evaluation of rapid methods for the determination of okadaic acid in mussels</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>L.CROCI, A.STACCHINI, L.COZZI, G.CICCAGLIONI, F.MAZZEI, F.BOTRÈ AND L.TOTI. 2001.
Aims: Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the official mouse bioassay in monitoring for okadaic acid (OA) levels in mussels.
Methods and Results: Diarrhoetic shellfish poison‐contaminated mussels from the Adriatic Sea were assayed in parallel by means of the mouse bioassay and both alternative methods. Both the cytotoxicity test and the biosensor test showed high sensitivity (OA 0·01 mg g–1 hepatopancreas and 0·002 mg g–1 hepatopancreas, respectively) and a high correlation with the mouse bioassay (r=0·932, P < 0·001 and r=− 0·850, P < 0·001, respectively).
Conclusions: Both methods are efficacious, quick, inexpensive and provide data on the amount of toxin present in mussels.
Significance and Impact of the Study: Both methods, besides allowing the simultaneous assay of a great number of samples, comply with the ethical need to reduce the use of animals in the laboratory.</description><subject>Acid Phosphatase - metabolism</subject><subject>Animal Testing Alternatives</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Assay - economics</subject><subject>Biological Assay - methods</subject><subject>Biosensing Techniques - economics</subject><subject>Biosensing Techniques - methods</subject><subject>Bivalvia - chemistry</subject><subject>Calibration</subject><subject>Fish and seafood industries</subject><subject>Food industries</subject><subject>Food toxicology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose Oxidase - metabolism</subject><subject>Haplorhini</subject><subject>Mice</subject><subject>Oceans and Seas</subject><subject>Okadaic Acid - analysis</subject><subject>Okadaic Acid - toxicity</subject><subject>Sensitivity and Specificity</subject><subject>Time Factors</subject><subject>Toxicity Tests - economics</subject><subject>Toxicity Tests - methods</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMtOwzAQRS0EoqXwC8iCdYIfsZ0sWFRVeamIDawtx3bUhDyKnUD79yRtVLasZjRzZq50AIAYhRhF_K4IMeUsIFyQkCCEQ4QJjsPtCZgeF6f7PgoYEmQCLrwvepAixs_BBGPMGCZsClbLb1V2qs2bGjYZdGqTG1jZdt0YD7PGwXZtobGtdVVeH7HmUxmVa6h0T-c1rDrvbekvwVmmSm-vxjoDHw_L98VTsHp7fF7MV4GOCIoDQS2lAifW0kxpkgplhE2FoGnEVJISlCBhODY6SWPNVT9W3EYZz7BmcYIMnYGbw9-Na74661tZNJ2r-0hJKElowhLaQ_EB0q7x3tlMblxeKbeTGMnBoizkIEsOsuRgUe4tym1_ej3-79LKmr_DUVsP3I6A8lqVmVO1zv2Ri3kcRaSn7g_UT17a3b_j5cv8dejoLw0ZjIA</recordid><startdate>200101</startdate><enddate>200101</enddate><creator>Croci, L.</creator><creator>Stacchini, A.</creator><creator>Cozzi, L.</creator><creator>Ciccaglioni, G.</creator><creator>Mazzei, F.</creator><creator>Botrè, F.</creator><creator>Toti, L.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>200101</creationdate><title>Evaluation of rapid methods for the determination of okadaic acid in mussels</title><author>Croci, L. ; Stacchini, A. ; Cozzi, L. ; Ciccaglioni, G. ; Mazzei, F. ; Botrè, F. ; Toti, L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4208-73e33719ee3fac2b7ad7eb773b45a9b20907d61dc9b8c6a3b4a6e4f6f1c5890d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Acid Phosphatase - metabolism</topic><topic>Animal Testing Alternatives</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Assay - economics</topic><topic>Biological Assay - methods</topic><topic>Biosensing Techniques - economics</topic><topic>Biosensing Techniques - methods</topic><topic>Bivalvia - chemistry</topic><topic>Calibration</topic><topic>Fish and seafood industries</topic><topic>Food industries</topic><topic>Food toxicology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose Oxidase - metabolism</topic><topic>Haplorhini</topic><topic>Mice</topic><topic>Oceans and Seas</topic><topic>Okadaic Acid - analysis</topic><topic>Okadaic Acid - toxicity</topic><topic>Sensitivity and Specificity</topic><topic>Time Factors</topic><topic>Toxicity Tests - economics</topic><topic>Toxicity Tests - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Croci, L.</creatorcontrib><creatorcontrib>Stacchini, A.</creatorcontrib><creatorcontrib>Cozzi, L.</creatorcontrib><creatorcontrib>Ciccaglioni, G.</creatorcontrib><creatorcontrib>Mazzei, F.</creatorcontrib><creatorcontrib>Botrè, F.</creatorcontrib><creatorcontrib>Toti, L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Croci, L.</au><au>Stacchini, A.</au><au>Cozzi, L.</au><au>Ciccaglioni, G.</au><au>Mazzei, F.</au><au>Botrè, F.</au><au>Toti, L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of rapid methods for the determination of okadaic acid in mussels</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2001-01</date><risdate>2001</risdate><volume>90</volume><issue>1</issue><spage>73</spage><epage>77</epage><pages>73-77</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><coden>JAMIFK</coden><abstract>L.CROCI, A.STACCHINI, L.COZZI, G.CICCAGLIONI, F.MAZZEI, F.BOTRÈ AND L.TOTI. 2001.
Aims: Two different screening methods, a Buffalo Green Monkey cytotoxicity test and a biosensor test, have been considered to replace the official mouse bioassay in monitoring for okadaic acid (OA) levels in mussels.
Methods and Results: Diarrhoetic shellfish poison‐contaminated mussels from the Adriatic Sea were assayed in parallel by means of the mouse bioassay and both alternative methods. Both the cytotoxicity test and the biosensor test showed high sensitivity (OA 0·01 mg g–1 hepatopancreas and 0·002 mg g–1 hepatopancreas, respectively) and a high correlation with the mouse bioassay (r=0·932, P < 0·001 and r=− 0·850, P < 0·001, respectively).
Conclusions: Both methods are efficacious, quick, inexpensive and provide data on the amount of toxin present in mussels.
Significance and Impact of the Study: Both methods, besides allowing the simultaneous assay of a great number of samples, comply with the ethical need to reduce the use of animals in the laboratory.</abstract><cop>Oxford UK</cop><pub>Blackwell Science Ltd</pub><pmid>11155125</pmid><doi>10.1046/j.1365-2672.2001.01218.x</doi><tpages>5</tpages></addata></record> |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Acid Phosphatase - metabolism Animal Testing Alternatives Animals Biological and medical sciences Biological Assay - economics Biological Assay - methods Biosensing Techniques - economics Biosensing Techniques - methods Bivalvia - chemistry Calibration Fish and seafood industries Food industries Food toxicology Fundamental and applied biological sciences. Psychology Glucose Oxidase - metabolism Haplorhini Mice Oceans and Seas Okadaic Acid - analysis Okadaic Acid - toxicity Sensitivity and Specificity Time Factors Toxicity Tests - economics Toxicity Tests - methods |
| title | Evaluation of rapid methods for the determination of okadaic acid in mussels |
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