In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation
BACKGROUND Despite the growing importance of probiotics apparent health benefits, an impediment to the use of new probiotic cultures is their safety. Hence there is a need to strictly examine the biosafety as well as health benefits of probiotics in in vivo model systems. RESULTS In this study, two...
Gespeichert in:
| Veröffentlicht in: | Journal of the science of food and agriculture 2020-01, Vol.100 (2), p.705-713 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 713 |
|---|---|
| container_issue | 2 |
| container_start_page | 705 |
| container_title | Journal of the science of food and agriculture |
| container_volume | 100 |
| creator | Thumu, Surya Chandra Rao Halami, Prakash M |
| description | BACKGROUND
Despite the growing importance of probiotics apparent health benefits, an impediment to the use of new probiotic cultures is their safety. Hence there is a need to strictly examine the biosafety as well as health benefits of probiotics in in vivo model systems.
RESULTS
In this study, two lactic acid bacterial (LAB) cultures Lactobacillus fermentum NCMR 2826 and FIX proven for their in vitro probiotic properties were investigated for their in vivo safety in Wistar rats. An acute toxicity study (14 days) with a high dose of biomass (1016 colony‐forming units (CFU) mL−1) followed by a subchronic test for 13 weeks with oral feeding of the probiotic cultures in three different doses (107, 108 and 1010 CFU mL−1) on a daily basis revealed the safety of the L. fermentum cultures. The probiotic feeding had no toxic effects on survival, body weight and food consumption with any of the dosages used throughout the treatment period. No statistically significant changes in relative organ weights and serum biochemical and hematological indices were found between the control and the probiotic fed animals. In addition to the safety attributes, the L. fermentum culture fed rats showed reduced serum cholesterol levels, macrovesicular steatosis and hepatocyte ballooning compared with control animals. Further, quantification of intestinal microbiota using real‐time polymerase chain reaction (PCR) analysis from animal feces indicated a significant increase and stability of Lactobacillus and Bifidobacterium counts but a decrease of Escherichia coli numbers.
CONCLUSION
This study of safety and beneficial features highlights the use of the two native L. fermentum isolates as potential probiotic food supplements. © 2019 Society of Chemical Industry |
| doi_str_mv | 10.1002/jsfa.10071 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_journals_2325673882</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2325673882</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3931-ba2d2383cb0467cf835c4995a4e868d3c1dd38a117565b8665d815d1befba2133</originalsourceid><addsrcrecordid>eNp9kbFOwzAURS0EoqWw8AHIEhsiYMd14oxVRaGoEgMwR47tUFdOXOykVTc-gYEv5Etw2sLI9K70ju-78gXgHKMbjFB8u_Al71SKD0AfoyyNEMLoEPTDMo4oHsY9cOL9AiGUZUlyDHoE0yzItA--pjVc6ZWFnpeq2UDuvfK-UnUDbQlnXDS24EIb03pYKtct2gr6xnFd-2uoVty0vNG27vBmrrSDYm6N8o1y1nx_fBq7Vk7Xb5AX2ujuQi2hrptA6JobWGnhbKE7ZWVrtl6n4Kjkxquz_RyA18ndy_ghmj3dT8ejWSRIRnBU8FjGhBFRoGGSipIRKoZZRvlQsYRJIrCUhHGMU5rQgiUJlQxTiQtVhqeYkAG43PkunX1vQ6J8YVsXUvk8JjFNUsJYHKirHRWCeu9UmS-drrjb5BjlXQF5V0C-LSDAF3vLtqiU_EN_fzwAeAestVGbf6zyx-fJaGf6A-iRlSI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2325673882</pqid></control><display><type>article</type><title>In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Thumu, Surya Chandra Rao ; Halami, Prakash M</creator><creatorcontrib>Thumu, Surya Chandra Rao ; Halami, Prakash M</creatorcontrib><description>BACKGROUND
Despite the growing importance of probiotics apparent health benefits, an impediment to the use of new probiotic cultures is their safety. Hence there is a need to strictly examine the biosafety as well as health benefits of probiotics in in vivo model systems.
RESULTS
In this study, two lactic acid bacterial (LAB) cultures Lactobacillus fermentum NCMR 2826 and FIX proven for their in vitro probiotic properties were investigated for their in vivo safety in Wistar rats. An acute toxicity study (14 days) with a high dose of biomass (1016 colony‐forming units (CFU) mL−1) followed by a subchronic test for 13 weeks with oral feeding of the probiotic cultures in three different doses (107, 108 and 1010 CFU mL−1) on a daily basis revealed the safety of the L. fermentum cultures. The probiotic feeding had no toxic effects on survival, body weight and food consumption with any of the dosages used throughout the treatment period. No statistically significant changes in relative organ weights and serum biochemical and hematological indices were found between the control and the probiotic fed animals. In addition to the safety attributes, the L. fermentum culture fed rats showed reduced serum cholesterol levels, macrovesicular steatosis and hepatocyte ballooning compared with control animals. Further, quantification of intestinal microbiota using real‐time polymerase chain reaction (PCR) analysis from animal feces indicated a significant increase and stability of Lactobacillus and Bifidobacterium counts but a decrease of Escherichia coli numbers.
CONCLUSION
This study of safety and beneficial features highlights the use of the two native L. fermentum isolates as potential probiotic food supplements. © 2019 Society of Chemical Industry</description><identifier>ISSN: 0022-5142</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.10071</identifier><identifier>PMID: 31599967</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Acute toxicity ; Animals ; Anticholesteremic Agents - administration & dosage ; Anticholesteremic Agents - metabolism ; Bacteria - classification ; Bacteria - drug effects ; Bacteria - genetics ; Bacteria - isolation & purification ; Biocompatibility ; Body weight ; Cholesterol ; Cholesterol - blood ; Cholesterol - metabolism ; cholesterol lowering ; Dietary supplements ; Dosage ; E coli ; Feeding ; Food ; Food consumption ; Gastrointestinal Microbiome - drug effects ; gut microbial modulation ; Hematology ; In vivo methods and tests ; in vivo safety ; Intestinal microflora ; Intestine ; Intestines - drug effects ; Intestines - microbiology ; Lactic acid ; Lactobacillus fermentum ; Lactobacillus fermentum - growth & development ; Lactobacillus fermentum - metabolism ; Male ; Microbiota ; Microorganisms ; Organic chemistry ; Polymerase chain reaction ; Probiotics ; Probiotics - administration & dosage ; Probiotics - metabolism ; Rats ; Rats, Wistar ; Safety ; Stability analysis ; Statistical analysis ; Steatosis ; Toxicity ; Yogurt</subject><ispartof>Journal of the science of food and agriculture, 2020-01, Vol.100 (2), p.705-713</ispartof><rights>2019 Society of Chemical Industry</rights><rights>2019 Society of Chemical Industry.</rights><rights>Copyright © 2020 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3931-ba2d2383cb0467cf835c4995a4e868d3c1dd38a117565b8665d815d1befba2133</citedby><cites>FETCH-LOGICAL-c3931-ba2d2383cb0467cf835c4995a4e868d3c1dd38a117565b8665d815d1befba2133</cites><orcidid>0000-0002-0293-1891</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.10071$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.10071$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31599967$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thumu, Surya Chandra Rao</creatorcontrib><creatorcontrib>Halami, Prakash M</creatorcontrib><title>In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Despite the growing importance of probiotics apparent health benefits, an impediment to the use of new probiotic cultures is their safety. Hence there is a need to strictly examine the biosafety as well as health benefits of probiotics in in vivo model systems.
RESULTS
In this study, two lactic acid bacterial (LAB) cultures Lactobacillus fermentum NCMR 2826 and FIX proven for their in vitro probiotic properties were investigated for their in vivo safety in Wistar rats. An acute toxicity study (14 days) with a high dose of biomass (1016 colony‐forming units (CFU) mL−1) followed by a subchronic test for 13 weeks with oral feeding of the probiotic cultures in three different doses (107, 108 and 1010 CFU mL−1) on a daily basis revealed the safety of the L. fermentum cultures. The probiotic feeding had no toxic effects on survival, body weight and food consumption with any of the dosages used throughout the treatment period. No statistically significant changes in relative organ weights and serum biochemical and hematological indices were found between the control and the probiotic fed animals. In addition to the safety attributes, the L. fermentum culture fed rats showed reduced serum cholesterol levels, macrovesicular steatosis and hepatocyte ballooning compared with control animals. Further, quantification of intestinal microbiota using real‐time polymerase chain reaction (PCR) analysis from animal feces indicated a significant increase and stability of Lactobacillus and Bifidobacterium counts but a decrease of Escherichia coli numbers.
CONCLUSION
This study of safety and beneficial features highlights the use of the two native L. fermentum isolates as potential probiotic food supplements. © 2019 Society of Chemical Industry</description><subject>Acute toxicity</subject><subject>Animals</subject><subject>Anticholesteremic Agents - administration & dosage</subject><subject>Anticholesteremic Agents - metabolism</subject><subject>Bacteria - classification</subject><subject>Bacteria - drug effects</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Biocompatibility</subject><subject>Body weight</subject><subject>Cholesterol</subject><subject>Cholesterol - blood</subject><subject>Cholesterol - metabolism</subject><subject>cholesterol lowering</subject><subject>Dietary supplements</subject><subject>Dosage</subject><subject>E coli</subject><subject>Feeding</subject><subject>Food</subject><subject>Food consumption</subject><subject>Gastrointestinal Microbiome - drug effects</subject><subject>gut microbial modulation</subject><subject>Hematology</subject><subject>In vivo methods and tests</subject><subject>in vivo safety</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Intestines - drug effects</subject><subject>Intestines - microbiology</subject><subject>Lactic acid</subject><subject>Lactobacillus fermentum</subject><subject>Lactobacillus fermentum - growth & development</subject><subject>Lactobacillus fermentum - metabolism</subject><subject>Male</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Organic chemistry</subject><subject>Polymerase chain reaction</subject><subject>Probiotics</subject><subject>Probiotics - administration & dosage</subject><subject>Probiotics - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Safety</subject><subject>Stability analysis</subject><subject>Statistical analysis</subject><subject>Steatosis</subject><subject>Toxicity</subject><subject>Yogurt</subject><issn>0022-5142</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbFOwzAURS0EoqWw8AHIEhsiYMd14oxVRaGoEgMwR47tUFdOXOykVTc-gYEv5Etw2sLI9K70ju-78gXgHKMbjFB8u_Al71SKD0AfoyyNEMLoEPTDMo4oHsY9cOL9AiGUZUlyDHoE0yzItA--pjVc6ZWFnpeq2UDuvfK-UnUDbQlnXDS24EIb03pYKtct2gr6xnFd-2uoVty0vNG27vBmrrSDYm6N8o1y1nx_fBq7Vk7Xb5AX2ujuQi2hrptA6JobWGnhbKE7ZWVrtl6n4Kjkxquz_RyA18ndy_ghmj3dT8ejWSRIRnBU8FjGhBFRoGGSipIRKoZZRvlQsYRJIrCUhHGMU5rQgiUJlQxTiQtVhqeYkAG43PkunX1vQ6J8YVsXUvk8JjFNUsJYHKirHRWCeu9UmS-drrjb5BjlXQF5V0C-LSDAF3vLtqiU_EN_fzwAeAestVGbf6zyx-fJaGf6A-iRlSI</recordid><startdate>20200130</startdate><enddate>20200130</enddate><creator>Thumu, Surya Chandra Rao</creator><creator>Halami, Prakash M</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><orcidid>https://orcid.org/0000-0002-0293-1891</orcidid></search><sort><creationdate>20200130</creationdate><title>In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation</title><author>Thumu, Surya Chandra Rao ; Halami, Prakash M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3931-ba2d2383cb0467cf835c4995a4e868d3c1dd38a117565b8665d815d1befba2133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Acute toxicity</topic><topic>Animals</topic><topic>Anticholesteremic Agents - administration & dosage</topic><topic>Anticholesteremic Agents - metabolism</topic><topic>Bacteria - classification</topic><topic>Bacteria - drug effects</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Biocompatibility</topic><topic>Body weight</topic><topic>Cholesterol</topic><topic>Cholesterol - blood</topic><topic>Cholesterol - metabolism</topic><topic>cholesterol lowering</topic><topic>Dietary supplements</topic><topic>Dosage</topic><topic>E coli</topic><topic>Feeding</topic><topic>Food</topic><topic>Food consumption</topic><topic>Gastrointestinal Microbiome - drug effects</topic><topic>gut microbial modulation</topic><topic>Hematology</topic><topic>In vivo methods and tests</topic><topic>in vivo safety</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Intestines - drug effects</topic><topic>Intestines - microbiology</topic><topic>Lactic acid</topic><topic>Lactobacillus fermentum</topic><topic>Lactobacillus fermentum - growth & development</topic><topic>Lactobacillus fermentum - metabolism</topic><topic>Male</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Organic chemistry</topic><topic>Polymerase chain reaction</topic><topic>Probiotics</topic><topic>Probiotics - administration & dosage</topic><topic>Probiotics - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Safety</topic><topic>Stability analysis</topic><topic>Statistical analysis</topic><topic>Steatosis</topic><topic>Toxicity</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thumu, Surya Chandra Rao</creatorcontrib><creatorcontrib>Halami, Prakash M</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thumu, Surya Chandra Rao</au><au>Halami, Prakash M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2020-01-30</date><risdate>2020</risdate><volume>100</volume><issue>2</issue><spage>705</spage><epage>713</epage><pages>705-713</pages><issn>0022-5142</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Despite the growing importance of probiotics apparent health benefits, an impediment to the use of new probiotic cultures is their safety. Hence there is a need to strictly examine the biosafety as well as health benefits of probiotics in in vivo model systems.
RESULTS
In this study, two lactic acid bacterial (LAB) cultures Lactobacillus fermentum NCMR 2826 and FIX proven for their in vitro probiotic properties were investigated for their in vivo safety in Wistar rats. An acute toxicity study (14 days) with a high dose of biomass (1016 colony‐forming units (CFU) mL−1) followed by a subchronic test for 13 weeks with oral feeding of the probiotic cultures in three different doses (107, 108 and 1010 CFU mL−1) on a daily basis revealed the safety of the L. fermentum cultures. The probiotic feeding had no toxic effects on survival, body weight and food consumption with any of the dosages used throughout the treatment period. No statistically significant changes in relative organ weights and serum biochemical and hematological indices were found between the control and the probiotic fed animals. In addition to the safety attributes, the L. fermentum culture fed rats showed reduced serum cholesterol levels, macrovesicular steatosis and hepatocyte ballooning compared with control animals. Further, quantification of intestinal microbiota using real‐time polymerase chain reaction (PCR) analysis from animal feces indicated a significant increase and stability of Lactobacillus and Bifidobacterium counts but a decrease of Escherichia coli numbers.
CONCLUSION
This study of safety and beneficial features highlights the use of the two native L. fermentum isolates as potential probiotic food supplements. © 2019 Society of Chemical Industry</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>31599967</pmid><doi>10.1002/jsfa.10071</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0293-1891</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-5142 |
| ispartof | Journal of the science of food and agriculture, 2020-01, Vol.100 (2), p.705-713 |
| issn | 0022-5142 1097-0010 |
| language | eng |
| recordid | cdi_proquest_journals_2325673882 |
| source | MEDLINE; Wiley Online Library All Journals |
| subjects | Acute toxicity Animals Anticholesteremic Agents - administration & dosage Anticholesteremic Agents - metabolism Bacteria - classification Bacteria - drug effects Bacteria - genetics Bacteria - isolation & purification Biocompatibility Body weight Cholesterol Cholesterol - blood Cholesterol - metabolism cholesterol lowering Dietary supplements Dosage E coli Feeding Food Food consumption Gastrointestinal Microbiome - drug effects gut microbial modulation Hematology In vivo methods and tests in vivo safety Intestinal microflora Intestine Intestines - drug effects Intestines - microbiology Lactic acid Lactobacillus fermentum Lactobacillus fermentum - growth & development Lactobacillus fermentum - metabolism Male Microbiota Microorganisms Organic chemistry Polymerase chain reaction Probiotics Probiotics - administration & dosage Probiotics - metabolism Rats Rats, Wistar Safety Stability analysis Statistical analysis Steatosis Toxicity Yogurt |
| title | In vivo safety assessment of Lactobacillus fermentum strains, evaluation of their cholesterol‐lowering ability and intestinal microbial modulation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T11%3A24%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=In%20vivo%20safety%20assessment%20of%20Lactobacillus%20fermentum%20strains,%20evaluation%20of%20their%20cholesterol%E2%80%90lowering%20ability%20and%20intestinal%20microbial%20modulation&rft.jtitle=Journal%20of%20the%20science%20of%20food%20and%20agriculture&rft.au=Thumu,%20Surya%20Chandra%20Rao&rft.date=2020-01-30&rft.volume=100&rft.issue=2&rft.spage=705&rft.epage=713&rft.pages=705-713&rft.issn=0022-5142&rft.eissn=1097-0010&rft_id=info:doi/10.1002/jsfa.10071&rft_dat=%3Cproquest_cross%3E2325673882%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2325673882&rft_id=info:pmid/31599967&rfr_iscdi=true |