Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis
Sugar nucleotides, the activated forms of monosaccharides, are used as donor substrates by glycosyltransferases in the in vivo biosynthesis of polysaccharides, proteoglycans and glycoglycerolipids. In mammals, uridine 5'-diphosphate-glucose (UDP-Glc) and uridine 5'-diphosphate-glucuronic a...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2019-11, Vol.55 (6), p.631-638 |
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| creator | Zang, L. X. Du, R. R. Zang, H. C. Wang, F. S. Sheng, J. Z. |
| description | Sugar nucleotides, the activated forms of monosaccharides, are used as donor substrates by glycosyltransferases in the in vivo biosynthesis of polysaccharides, proteoglycans and glycoglycerolipids. In mammals, uridine 5'-diphosphate-glucose (UDP-Glc) and uridine 5'-diphosphate-glucuronic acid (UDP-GlcA) are common building blocks of glycans and glycoconjugates. The commercial demand for these high-energy donors is increasing, while the understanding and application of enzymatic glycan synthesis has leapt forward in recent years. To produce valuable UDP-GlcA in a cost-effective way, here, UDP-sugar pyrophosphorylase from
Arabidopsis thaliana
was constitutively expressed in
Pichia pastoris
and secreted into the extracellular medium. Under these conditions, the synthesis of 4.2 g UDP-GlcA or 5.5 g UDP-Glc per liter of culture was revealed in the culture medium, without any need for purification. An anion exchange chromatography purification method for UDP-sugars was also developed
.
This route opens a door to large-scale production of the cheaper UDP-GlcA. |
| doi_str_mv | 10.1134/S0003683819060152 |
| format | Article |
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Arabidopsis thaliana
was constitutively expressed in
Pichia pastoris
and secreted into the extracellular medium. Under these conditions, the synthesis of 4.2 g UDP-GlcA or 5.5 g UDP-Glc per liter of culture was revealed in the culture medium, without any need for purification. An anion exchange chromatography purification method for UDP-sugars was also developed
.
This route opens a door to large-scale production of the cheaper UDP-GlcA.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683819060152</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Anion exchange ; Anion exchanging ; Arabidopsis thaliana ; Biochemistry ; Biomedical and Life Sciences ; Biosynthesis ; Glucose ; Glycan ; Glycoconjugates ; Life Sciences ; Medical Microbiology ; Microbiology ; Monosaccharides ; Nucleotides ; Pichia pastoris ; Polysaccharides ; Proteoglycans ; Purification ; Saccharides ; Substrates ; Sugar ; Uridine ; Yeast</subject><ispartof>Applied biochemistry and microbiology, 2019-11, Vol.55 (6), p.631-638</ispartof><rights>Pleiades Publishing, Inc. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-9fd01cd831681f0bf86e3fe9132b86bdd9ca0e4a95fda83e8e1cde1349c08d0c3</citedby><cites>FETCH-LOGICAL-c353t-9fd01cd831681f0bf86e3fe9132b86bdd9ca0e4a95fda83e8e1cde1349c08d0c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683819060152$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683819060152$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Zang, L. X.</creatorcontrib><creatorcontrib>Du, R. R.</creatorcontrib><creatorcontrib>Zang, H. C.</creatorcontrib><creatorcontrib>Wang, F. S.</creatorcontrib><creatorcontrib>Sheng, J. Z.</creatorcontrib><title>Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>Sugar nucleotides, the activated forms of monosaccharides, are used as donor substrates by glycosyltransferases in the in vivo biosynthesis of polysaccharides, proteoglycans and glycoglycerolipids. In mammals, uridine 5'-diphosphate-glucose (UDP-Glc) and uridine 5'-diphosphate-glucuronic acid (UDP-GlcA) are common building blocks of glycans and glycoconjugates. The commercial demand for these high-energy donors is increasing, while the understanding and application of enzymatic glycan synthesis has leapt forward in recent years. To produce valuable UDP-GlcA in a cost-effective way, here, UDP-sugar pyrophosphorylase from
Arabidopsis thaliana
was constitutively expressed in
Pichia pastoris
and secreted into the extracellular medium. Under these conditions, the synthesis of 4.2 g UDP-GlcA or 5.5 g UDP-Glc per liter of culture was revealed in the culture medium, without any need for purification. An anion exchange chromatography purification method for UDP-sugars was also developed
.
This route opens a door to large-scale production of the cheaper UDP-GlcA.</description><subject>Anion exchange</subject><subject>Anion exchanging</subject><subject>Arabidopsis thaliana</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biosynthesis</subject><subject>Glucose</subject><subject>Glycan</subject><subject>Glycoconjugates</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Monosaccharides</subject><subject>Nucleotides</subject><subject>Pichia pastoris</subject><subject>Polysaccharides</subject><subject>Proteoglycans</subject><subject>Purification</subject><subject>Saccharides</subject><subject>Substrates</subject><subject>Sugar</subject><subject>Uridine</subject><subject>Yeast</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kV1LwzAUhoMoOKc_wLuA19V8rCW9LHPqYGBh7rqk-VgzalKT9qL_xp9quileiBchHM7zvCfkAHCL0T3GdPGwRQjRjFGGc5QhnJIzMMMZYglFZHEOZlM7mfqX4CqEQyzzjOUz8Fl6JwfRG2eh07DwvDbSdcEE2De8NdxyuHssk-2w5x6Wo3dd40I8fmx5ULAeYWlEYzjseOidjx63Eq77AIuua43gx2hj4UprI4yy_THvuR2Ei_4E_9SDd9YIWAgj4Xa0faPiM67BheZtUDff9xzsnlZvy5dk8_q8XhabRNCU9kmuJcJCMoozhjWqNcsU1SrHlNQsq6XMBUdqwfNUS86oYirSKv5cLhCTSNA5uDvldt59DCr01cEN3saRFaGEUEbSjEQKnyjhXQhe6arz5p37scKomhZR_VlEdMjJCZG1e-V_k_-XvgAHOYzk</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Zang, L. X.</creator><creator>Du, R. R.</creator><creator>Zang, H. C.</creator><creator>Wang, F. S.</creator><creator>Sheng, J. Z.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20191101</creationdate><title>Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis</title><author>Zang, L. X. ; Du, R. R. ; Zang, H. C. ; Wang, F. S. ; Sheng, J. Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-9fd01cd831681f0bf86e3fe9132b86bdd9ca0e4a95fda83e8e1cde1349c08d0c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anion exchange</topic><topic>Anion exchanging</topic><topic>Arabidopsis thaliana</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biosynthesis</topic><topic>Glucose</topic><topic>Glycan</topic><topic>Glycoconjugates</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Monosaccharides</topic><topic>Nucleotides</topic><topic>Pichia pastoris</topic><topic>Polysaccharides</topic><topic>Proteoglycans</topic><topic>Purification</topic><topic>Saccharides</topic><topic>Substrates</topic><topic>Sugar</topic><topic>Uridine</topic><topic>Yeast</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zang, L. X.</creatorcontrib><creatorcontrib>Du, R. R.</creatorcontrib><creatorcontrib>Zang, H. C.</creatorcontrib><creatorcontrib>Wang, F. S.</creatorcontrib><creatorcontrib>Sheng, J. Z.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zang, L. X.</au><au>Du, R. R.</au><au>Zang, H. C.</au><au>Wang, F. S.</au><au>Sheng, J. Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2019-11-01</date><risdate>2019</risdate><volume>55</volume><issue>6</issue><spage>631</spage><epage>638</epage><pages>631-638</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>Sugar nucleotides, the activated forms of monosaccharides, are used as donor substrates by glycosyltransferases in the in vivo biosynthesis of polysaccharides, proteoglycans and glycoglycerolipids. In mammals, uridine 5'-diphosphate-glucose (UDP-Glc) and uridine 5'-diphosphate-glucuronic acid (UDP-GlcA) are common building blocks of glycans and glycoconjugates. The commercial demand for these high-energy donors is increasing, while the understanding and application of enzymatic glycan synthesis has leapt forward in recent years. To produce valuable UDP-GlcA in a cost-effective way, here, UDP-sugar pyrophosphorylase from
Arabidopsis thaliana
was constitutively expressed in
Pichia pastoris
and secreted into the extracellular medium. Under these conditions, the synthesis of 4.2 g UDP-GlcA or 5.5 g UDP-Glc per liter of culture was revealed in the culture medium, without any need for purification. An anion exchange chromatography purification method for UDP-sugars was also developed
.
This route opens a door to large-scale production of the cheaper UDP-GlcA.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683819060152</doi><tpages>8</tpages></addata></record> |
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| subjects | Anion exchange Anion exchanging Arabidopsis thaliana Biochemistry Biomedical and Life Sciences Biosynthesis Glucose Glycan Glycoconjugates Life Sciences Medical Microbiology Microbiology Monosaccharides Nucleotides Pichia pastoris Polysaccharides Proteoglycans Purification Saccharides Substrates Sugar Uridine Yeast |
| title | Production of Arabidopsis thaliana UDP-Sugar Pyrophosphorylase by Pichia pastoris and Its Application in Efficient UDP-Glucose and UDP-Glucuronic Acid Synthesis |
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