Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion
It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan-arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoa...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2019-11, Vol.55 (6), p.677-683 |
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| creator | Semenova, M. V. Rozhkova, A. M. Osipov, D. O. Satrutdinov, A. D. Sinitsyna, O. A. Rubtsova, E. A. Kondrateva, E. G. Sinitsyn, A. P. |
| description | It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan-arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were determined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61–68%, an arabinose yield of 94%, and a glucose yield of 63–79%. The optimal complex from dry multienzyme preparations (EP) of cellulases, hemicellulases, and pectinases, which are produced by the fungal strains
Penicillium canescens
,
P. verruculosum,
and
Aspergillus foetidus
, enabled SBP hydrolysis based on arabinose and glucose yields close to 100% at an initial SBP concentration of 100–250 g/L and an EP concentration of 5–10 mg protein/g SBP after 24–48 h of hydrolysis. |
| doi_str_mv | 10.1134/S0003683819050119 |
| format | Article |
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Penicillium canescens
,
P. verruculosum,
and
Aspergillus foetidus
, enabled SBP hydrolysis based on arabinose and glucose yields close to 100% at an initial SBP concentration of 100–250 g/L and an EP concentration of 5–10 mg protein/g SBP after 24–48 h of hydrolysis.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683819050119</identifier><language>eng</language><publisher>Moscow: Pleiades Publishing</publisher><subject>Arabinofuranosidase ; Arabinose ; Biochemistry ; Biomedical and Life Sciences ; Cellobiase ; Cellobiohydrolase ; Conversion ; Endoglucanase ; Fungi ; Glucose ; Glucosidase ; Hemicellulases ; Hydrolysis ; Life Sciences ; Medical Microbiology ; Microbiology ; Multienzyme complexes ; Pectin ; Pectin lyase ; Pectinase ; Polygalacturonase ; Pulp ; Sugar ; Xylosidase ; β-Glucosidase</subject><ispartof>Applied biochemistry and microbiology, 2019-11, Vol.55 (6), p.677-683</ispartof><rights>Pleiades Publishing, Inc. 2019</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-a71ed25439500810b9739d1d563a6e81b4d9187a8549e74db3b6d542052736cf3</citedby><cites>FETCH-LOGICAL-c353t-a71ed25439500810b9739d1d563a6e81b4d9187a8549e74db3b6d542052736cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683819050119$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683819050119$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Semenova, M. V.</creatorcontrib><creatorcontrib>Rozhkova, A. M.</creatorcontrib><creatorcontrib>Osipov, D. O.</creatorcontrib><creatorcontrib>Satrutdinov, A. D.</creatorcontrib><creatorcontrib>Sinitsyna, O. A.</creatorcontrib><creatorcontrib>Rubtsova, E. A.</creatorcontrib><creatorcontrib>Kondrateva, E. G.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><title>Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion</title><title>Applied biochemistry and microbiology</title><addtitle>Appl Biochem Microbiol</addtitle><description>It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan-arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were determined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61–68%, an arabinose yield of 94%, and a glucose yield of 63–79%. The optimal complex from dry multienzyme preparations (EP) of cellulases, hemicellulases, and pectinases, which are produced by the fungal strains
Penicillium canescens
,
P. verruculosum,
and
Aspergillus foetidus
, enabled SBP hydrolysis based on arabinose and glucose yields close to 100% at an initial SBP concentration of 100–250 g/L and an EP concentration of 5–10 mg protein/g SBP after 24–48 h of hydrolysis.</description><subject>Arabinofuranosidase</subject><subject>Arabinose</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cellobiase</subject><subject>Cellobiohydrolase</subject><subject>Conversion</subject><subject>Endoglucanase</subject><subject>Fungi</subject><subject>Glucose</subject><subject>Glucosidase</subject><subject>Hemicellulases</subject><subject>Hydrolysis</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbiology</subject><subject>Multienzyme complexes</subject><subject>Pectin</subject><subject>Pectin lyase</subject><subject>Pectinase</subject><subject>Polygalacturonase</subject><subject>Pulp</subject><subject>Sugar</subject><subject>Xylosidase</subject><subject>β-Glucosidase</subject><issn>0003-6838</issn><issn>1608-3024</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kE9LAzEQxYMoWKsfwFvA82omk2ySo5ZalUKF6nnZP9nast2sya5QP72pFTyIp2F4v_eGeYRcArsGQHGzZIxhqlGDYZIBmCMygpTpBBkXx2S0l5O9fkrOQtjE1aTajMjT0ja27Neupa6m_Zuli65fb_OGTtvP3dbSidt2Lqy_idp5uhxWuad31vb0eWi6qLcf1ocon5OTOm-CvfiZY_J6P32ZPCTzxexxcjtPSpTYJ7kCW3Ep0EjGNLDCKDQVVDLFPLUaClEZ0CrXUhirRFVgkVZScCa5wrSscUyuDrmdd--DDX22cYNv48mMI-eotJYqUnCgSu9C8LbOOh__8rsMWLavLPtTWfTwgydEtl1Z_5v8v-kLXXRrYA</recordid><startdate>20191101</startdate><enddate>20191101</enddate><creator>Semenova, M. V.</creator><creator>Rozhkova, A. M.</creator><creator>Osipov, D. O.</creator><creator>Satrutdinov, A. D.</creator><creator>Sinitsyna, O. A.</creator><creator>Rubtsova, E. A.</creator><creator>Kondrateva, E. G.</creator><creator>Sinitsyn, A. P.</creator><general>Pleiades Publishing</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20191101</creationdate><title>Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion</title><author>Semenova, M. V. ; Rozhkova, A. M. ; Osipov, D. O. ; Satrutdinov, A. D. ; Sinitsyna, O. A. ; Rubtsova, E. A. ; Kondrateva, E. G. ; Sinitsyn, A. P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-a71ed25439500810b9739d1d563a6e81b4d9187a8549e74db3b6d542052736cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Arabinofuranosidase</topic><topic>Arabinose</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cellobiase</topic><topic>Cellobiohydrolase</topic><topic>Conversion</topic><topic>Endoglucanase</topic><topic>Fungi</topic><topic>Glucose</topic><topic>Glucosidase</topic><topic>Hemicellulases</topic><topic>Hydrolysis</topic><topic>Life Sciences</topic><topic>Medical Microbiology</topic><topic>Microbiology</topic><topic>Multienzyme complexes</topic><topic>Pectin</topic><topic>Pectin lyase</topic><topic>Pectinase</topic><topic>Polygalacturonase</topic><topic>Pulp</topic><topic>Sugar</topic><topic>Xylosidase</topic><topic>β-Glucosidase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Semenova, M. V.</creatorcontrib><creatorcontrib>Rozhkova, A. M.</creatorcontrib><creatorcontrib>Osipov, D. O.</creatorcontrib><creatorcontrib>Satrutdinov, A. D.</creatorcontrib><creatorcontrib>Sinitsyna, O. A.</creatorcontrib><creatorcontrib>Rubtsova, E. A.</creatorcontrib><creatorcontrib>Kondrateva, E. G.</creatorcontrib><creatorcontrib>Sinitsyn, A. P.</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Applied biochemistry and microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Semenova, M. V.</au><au>Rozhkova, A. M.</au><au>Osipov, D. O.</au><au>Satrutdinov, A. D.</au><au>Sinitsyna, O. A.</au><au>Rubtsova, E. A.</au><au>Kondrateva, E. G.</au><au>Sinitsyn, A. P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion</atitle><jtitle>Applied biochemistry and microbiology</jtitle><stitle>Appl Biochem Microbiol</stitle><date>2019-11-01</date><risdate>2019</risdate><volume>55</volume><issue>6</issue><spage>677</spage><epage>683</epage><pages>677-683</pages><issn>0003-6838</issn><eissn>1608-3024</eissn><abstract>It was shown that the presence of cellobiohydrolase, β-glucosidase, endoglucanase, arabinoxylan-arabinofuranhydrolase, pectin lyase, and polygalacturonase is necessary for efficient hydrolysis of sugar beet pulp (SBP). Optimal multienzyme complexes consisting of the same enzymes and additional endoarabinase or exoarabinase, endogalactanase, β-xylosidase, endoxylanase, and/or α-arabinofuranosidase were determined. These components enabled the conversion of SBP based on a yield of total reducing sugars (RSs) of 61–68%, an arabinose yield of 94%, and a glucose yield of 63–79%. The optimal complex from dry multienzyme preparations (EP) of cellulases, hemicellulases, and pectinases, which are produced by the fungal strains
Penicillium canescens
,
P. verruculosum,
and
Aspergillus foetidus
, enabled SBP hydrolysis based on arabinose and glucose yields close to 100% at an initial SBP concentration of 100–250 g/L and an EP concentration of 5–10 mg protein/g SBP after 24–48 h of hydrolysis.</abstract><cop>Moscow</cop><pub>Pleiades Publishing</pub><doi>10.1134/S0003683819050119</doi><tpages>7</tpages></addata></record> |
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| subjects | Arabinofuranosidase Arabinose Biochemistry Biomedical and Life Sciences Cellobiase Cellobiohydrolase Conversion Endoglucanase Fungi Glucose Glucosidase Hemicellulases Hydrolysis Life Sciences Medical Microbiology Microbiology Multienzyme complexes Pectin Pectin lyase Pectinase Polygalacturonase Pulp Sugar Xylosidase β-Glucosidase |
| title | Selection of the Optimal Enzyme Composition for Sugar Beet Pulp Conversion |
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