CCL5 protein level: influence on breast cancer staging and lymph nodes commitment
Many tumor cells express chemokines and chemokine receptors, and these molecules can contribute to distinct modes of metastasis processes. It is known that they play a crucial role in breast cancer (BC) tumorigenesis and progression. Considering this, it was investigated a possible role for C–Chemok...
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| Veröffentlicht in: | Molecular biology reports 2019-12, Vol.46 (6), p.6165-6170 |
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| creator | Derossi, Daniela Rudgeri Amarante, Marla Karine Guembarovski, Roberta Losi de Oliveira, Carlos Eduardo Coral Suzuki, Karen Mayumi Watanabe, Maria Angelica Ehara de Syllos Cólus, Ilce Mara |
| description | Many tumor cells express chemokines and chemokine receptors, and these molecules can contribute to distinct modes of metastasis processes. It is known that they play a crucial role in breast cancer (BC) tumorigenesis and progression. Considering this, it was investigated a possible role for C–Chemokine receptor type 5(
CCR5
) polymorphism
(rs333/delta32)
by conventional polymerase chain reaction (PCR) and CCL5 (C–C motif chemokine ligand 5) protein level by immunosorbent assay (ELISA) in 47 BC patients (resulting in 47 tumoral tissue samples and 47 adjacent normal tissue samples). There was a significant difference between CCL5 level in tumoral and adjacent normal tissues for the same BC patients (p |
| doi_str_mv | 10.1007/s11033-019-05051-8 |
| format | Article |
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CCR5
) polymorphism
(rs333/delta32)
by conventional polymerase chain reaction (PCR) and CCL5 (C–C motif chemokine ligand 5) protein level by immunosorbent assay (ELISA) in 47 BC patients (resulting in 47 tumoral tissue samples and 47 adjacent normal tissue samples). There was a significant difference between CCL5 level in tumoral and adjacent normal tissues for the same BC patients (p < 0.0001). A significant association was also found for CCL5 level in relation to lymph nodes commitment (p = 0.03). Likewise, there was a significant difference in CCL5 level from tumor tissue of stage III in relation to stage I (p < 0.02). On the other hand, it was verified that
CCR5
-
delta32
polymorphism presented no significant association in relation to CCL5 protein level. Considering the present findings, we suggest that CCL5 may be involved in BC staging and metastasis process.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-019-05051-8</identifier><identifier>PMID: 31691056</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Biomarkers ; Biomedical and Life Sciences ; Breast cancer ; breast neoplasms ; Breast Neoplasms - metabolism ; Breast Neoplasms - mortality ; Breast Neoplasms - pathology ; carcinogenesis ; CCR5 protein ; Chemokine CCL5 - metabolism ; Chemokine receptors ; Chemokines ; Enzyme-linked immunosorbent assay ; Female ; Gene polymorphism ; Histology ; Humans ; Immunohistochemistry ; Life Sciences ; ligands ; lymph ; Lymph nodes ; Lymphatic Metastasis ; Lymphatic system ; Metastases ; Metastasis ; Middle Aged ; Morphology ; Neoplasm Staging ; Original Article ; Polymerase chain reaction ; Polymorphism ; Prognosis ; protein content ; Proteins ; Tumor cells ; Tumorigenesis</subject><ispartof>Molecular biology reports, 2019-12, Vol.46 (6), p.6165-6170</ispartof><rights>Springer Nature B.V. 2019</rights><rights>Molecular Biology Reports is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-129613063ab57b1248e5eefd5abeb2dc1a3b4fe7b1b7d0e8bf685b1f3250baf03</citedby><cites>FETCH-LOGICAL-c408t-129613063ab57b1248e5eefd5abeb2dc1a3b4fe7b1b7d0e8bf685b1f3250baf03</cites><orcidid>0000-0001-8218-8518</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-019-05051-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-019-05051-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31691056$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Derossi, Daniela Rudgeri</creatorcontrib><creatorcontrib>Amarante, Marla Karine</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><creatorcontrib>de Oliveira, Carlos Eduardo Coral</creatorcontrib><creatorcontrib>Suzuki, Karen Mayumi</creatorcontrib><creatorcontrib>Watanabe, Maria Angelica Ehara</creatorcontrib><creatorcontrib>de Syllos Cólus, Ilce Mara</creatorcontrib><title>CCL5 protein level: influence on breast cancer staging and lymph nodes commitment</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>Many tumor cells express chemokines and chemokine receptors, and these molecules can contribute to distinct modes of metastasis processes. It is known that they play a crucial role in breast cancer (BC) tumorigenesis and progression. Considering this, it was investigated a possible role for C–Chemokine receptor type 5(
CCR5
) polymorphism
(rs333/delta32)
by conventional polymerase chain reaction (PCR) and CCL5 (C–C motif chemokine ligand 5) protein level by immunosorbent assay (ELISA) in 47 BC patients (resulting in 47 tumoral tissue samples and 47 adjacent normal tissue samples). There was a significant difference between CCL5 level in tumoral and adjacent normal tissues for the same BC patients (p < 0.0001). A significant association was also found for CCL5 level in relation to lymph nodes commitment (p = 0.03). Likewise, there was a significant difference in CCL5 level from tumor tissue of stage III in relation to stage I (p < 0.02). On the other hand, it was verified that
CCR5
-
delta32
polymorphism presented no significant association in relation to CCL5 protein level. Considering the present findings, we suggest that CCL5 may be involved in BC staging and metastasis process.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Biomarkers</subject><subject>Biomedical and Life Sciences</subject><subject>Breast cancer</subject><subject>breast neoplasms</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - mortality</subject><subject>Breast Neoplasms - pathology</subject><subject>carcinogenesis</subject><subject>CCR5 protein</subject><subject>Chemokine CCL5 - metabolism</subject><subject>Chemokine receptors</subject><subject>Chemokines</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Gene polymorphism</subject><subject>Histology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Life Sciences</subject><subject>ligands</subject><subject>lymph</subject><subject>Lymph nodes</subject><subject>Lymphatic Metastasis</subject><subject>Lymphatic system</subject><subject>Metastases</subject><subject>Metastasis</subject><subject>Middle Aged</subject><subject>Morphology</subject><subject>Neoplasm Staging</subject><subject>Original Article</subject><subject>Polymerase chain reaction</subject><subject>Polymorphism</subject><subject>Prognosis</subject><subject>protein content</subject><subject>Proteins</subject><subject>Tumor cells</subject><subject>Tumorigenesis</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kMFq3DAQhkVoSLZpXqCHIuglFzczlmTLuRXTNIGFEEjPQrLHWwdb3kh2Yd8-SnabQA89DcN888_wMfYZ4RsClJcREYTIAKsMFCjM9BFboSpFJqtSf2ArEICZ1ApP2ccYHwFAYqlO2KnAokJQxYrd1_Va8W2YZuo9H-gPDVe8992wkG-IT567QDbOvLGpDzzOdtP7Dbe-5cNu3P7mfmop8mYax34eyc-f2HFnh0jnh3rGfl3_eKhvsvXdz9v6-zprJOg5w7wqUEAhrFOlw1xqUkRdq6wjl7cNWuFkR2nkyhZIu67QymEncgXOdiDO2MU-Nz3_tFCczdjHhobBepqWaHKlsJKyFDqhX_9BH6cl-PSdyQVqqYTQKlH5nmrCFGOgzmxDP9qwMwjmRbjZCzdJuHkVbl6ivxyiFzdS-7by13ACxB6IaeQ3FN5v_yf2GYHaisc</recordid><startdate>20191201</startdate><enddate>20191201</enddate><creator>Derossi, Daniela Rudgeri</creator><creator>Amarante, Marla Karine</creator><creator>Guembarovski, Roberta Losi</creator><creator>de Oliveira, Carlos Eduardo Coral</creator><creator>Suzuki, Karen Mayumi</creator><creator>Watanabe, Maria Angelica Ehara</creator><creator>de Syllos Cólus, Ilce Mara</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0001-8218-8518</orcidid></search><sort><creationdate>20191201</creationdate><title>CCL5 protein level: influence on breast cancer staging and lymph nodes commitment</title><author>Derossi, Daniela Rudgeri ; Amarante, Marla Karine ; Guembarovski, Roberta Losi ; de Oliveira, Carlos Eduardo Coral ; Suzuki, Karen Mayumi ; Watanabe, Maria Angelica Ehara ; de Syllos Cólus, Ilce Mara</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-129613063ab57b1248e5eefd5abeb2dc1a3b4fe7b1b7d0e8bf685b1f3250baf03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Biomarkers</topic><topic>Biomedical and Life Sciences</topic><topic>Breast cancer</topic><topic>breast neoplasms</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - mortality</topic><topic>Breast Neoplasms - pathology</topic><topic>carcinogenesis</topic><topic>CCR5 protein</topic><topic>Chemokine CCL5 - metabolism</topic><topic>Chemokine receptors</topic><topic>Chemokines</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Gene polymorphism</topic><topic>Histology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Life Sciences</topic><topic>ligands</topic><topic>lymph</topic><topic>Lymph nodes</topic><topic>Lymphatic Metastasis</topic><topic>Lymphatic system</topic><topic>Metastases</topic><topic>Metastasis</topic><topic>Middle Aged</topic><topic>Morphology</topic><topic>Neoplasm Staging</topic><topic>Original Article</topic><topic>Polymerase chain reaction</topic><topic>Polymorphism</topic><topic>Prognosis</topic><topic>protein content</topic><topic>Proteins</topic><topic>Tumor cells</topic><topic>Tumorigenesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Derossi, Daniela Rudgeri</creatorcontrib><creatorcontrib>Amarante, Marla Karine</creatorcontrib><creatorcontrib>Guembarovski, Roberta Losi</creatorcontrib><creatorcontrib>de Oliveira, Carlos Eduardo Coral</creatorcontrib><creatorcontrib>Suzuki, Karen Mayumi</creatorcontrib><creatorcontrib>Watanabe, Maria Angelica Ehara</creatorcontrib><creatorcontrib>de Syllos Cólus, Ilce Mara</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Derossi, Daniela Rudgeri</au><au>Amarante, Marla Karine</au><au>Guembarovski, Roberta Losi</au><au>de Oliveira, Carlos Eduardo Coral</au><au>Suzuki, Karen Mayumi</au><au>Watanabe, Maria Angelica Ehara</au><au>de Syllos Cólus, Ilce Mara</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CCL5 protein level: influence on breast cancer staging and lymph nodes commitment</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2019-12-01</date><risdate>2019</risdate><volume>46</volume><issue>6</issue><spage>6165</spage><epage>6170</epage><pages>6165-6170</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Many tumor cells express chemokines and chemokine receptors, and these molecules can contribute to distinct modes of metastasis processes. It is known that they play a crucial role in breast cancer (BC) tumorigenesis and progression. Considering this, it was investigated a possible role for C–Chemokine receptor type 5(
CCR5
) polymorphism
(rs333/delta32)
by conventional polymerase chain reaction (PCR) and CCL5 (C–C motif chemokine ligand 5) protein level by immunosorbent assay (ELISA) in 47 BC patients (resulting in 47 tumoral tissue samples and 47 adjacent normal tissue samples). There was a significant difference between CCL5 level in tumoral and adjacent normal tissues for the same BC patients (p < 0.0001). A significant association was also found for CCL5 level in relation to lymph nodes commitment (p = 0.03). Likewise, there was a significant difference in CCL5 level from tumor tissue of stage III in relation to stage I (p < 0.02). On the other hand, it was verified that
CCR5
-
delta32
polymorphism presented no significant association in relation to CCL5 protein level. Considering the present findings, we suggest that CCL5 may be involved in BC staging and metastasis process.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>31691056</pmid><doi>10.1007/s11033-019-05051-8</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0001-8218-8518</orcidid></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Biomarkers Biomedical and Life Sciences Breast cancer breast neoplasms Breast Neoplasms - metabolism Breast Neoplasms - mortality Breast Neoplasms - pathology carcinogenesis CCR5 protein Chemokine CCL5 - metabolism Chemokine receptors Chemokines Enzyme-linked immunosorbent assay Female Gene polymorphism Histology Humans Immunohistochemistry Life Sciences ligands lymph Lymph nodes Lymphatic Metastasis Lymphatic system Metastases Metastasis Middle Aged Morphology Neoplasm Staging Original Article Polymerase chain reaction Polymorphism Prognosis protein content Proteins Tumor cells Tumorigenesis |
| title | CCL5 protein level: influence on breast cancer staging and lymph nodes commitment |
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